Restriction map for
pF1KA1780
(Circular) (Six-base) MAPSORT of: pF1KA1780.seq Check: 9293 from: 1 to: 6533
pF1KA1780 6533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 721 2229 2542 721
Size: 1508 313 4712
Fragments arranged by size:
4712 1508 313
AccI GT'mk_AC
Cuts at: 3571 3571
Size: 6533
Acc65I G'GTAC_C
Cuts at: 3549 3549
Size: 6533
AclI AA'CG_TT
Cuts at: 6373 6373
Size: 6533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 3296 4358 4790 5143 5805 2462
Size: 834 1062 432 353 662 3190
Fragments arranged by size:
3190 1062 834 662 432 353
AfeI AGC'GCT
Cuts at: 1153 1729 1153
Size: 576 5957
AflII C'TTAA_G
Cuts at: 2631 2631
Size: 6533
AflIII A'CryG_T
Cuts at: 970 1303 1651 2249 3885 5685 5966 970
Size: 333 348 598 1636 1800 281 1537
Fragments arranged by size:
1800 1636 1537 598 348 333 281
AleI CACnn'nnGTG
Cuts at: 1167 1167
Size: 6533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1770 2397 1770
Size: 627 5906
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1446 1478 1446
Size: 32 6501
AlwNI CAG_nnn'CTG
Cuts at: 235 521 2201 3838 5276 235
Size: 286 1680 1637 1438 1492
Fragments arranged by size:
1680 1637 1492 1438 286
ApaI G_GGCC'C
Cuts at: 1843 2018 2236 1843
Size: 175 218 6140
Fragments arranged by size:
6140 218 175
ApaLI G'TGCA_C
Cuts at: 1389 1655 1913 2372 5371 1389
Size: 266 258 459 2999 2551
Fragments arranged by size:
2999 2551 459 266 258
ApoI r'AATT_y
Cuts at: 269 2120 3144 3537 5886 6142 269
Size: 1851 1024 393 2349 256 660
Fragments arranged by size:
2349 1851 1024 660 393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6533
AvaI C'yCGr_G
Cuts at: 913 3553 913
Size: 2640 3893
BamHI G'GATC_C
Cuts at: 3396 3558 3396
Size: 162 6371
BanI G'GyrC_C
Cuts at: 560 661 1025 1930 2065 3549 3963 4211 4246
Size: 101 364 905 135 1484 414 248 35
Cuts at: 4246 560
Size: 2847
Fragments arranged by size:
2847 1484 905 414 364 248 135 101
35
BanII G_rGCy'C
Cuts at: 582 1184 1843 2018 2236 2327 3086 3547 4577
Size: 602 659 175 218 91 759 461 1030
Cuts at: 4577 582
Size: 2538
Fragments arranged by size:
2538 1030 759 659 602 461 218 175
91
BbeI G_GCGC'C
Cuts at: 2069 3967 4215 2069
Size: 1898 248 4387
Fragments arranged by size:
4387 1898 248
BbsI GAAGACnn'nnnn_
Cuts at: 200 367 200
Size: 167 6366
BbvCI CC'TCA_GC
Cuts at: 1826 1826
Size: 6533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3070 3104 3070
Size: 34 6499
BciVI GTATCCnnnnn_n'
Cuts at: 745 1003 4431 5487 6533 745
Size: 258 3428 1056 1046 745
Fragments arranged by size:
3428 1056 1046 745 258
BclI T'GATC_A
Cuts at: 264 531 2592 4053 264
Size: 267 2061 1461 2744
Fragments arranged by size:
2744 2061 1461 267
BglI GCCn_nnn'nGGC
Cuts at: 878 1700 878
Size: 822 5711
BglII A'GATC_T
Cuts at: 1340 2164 4048 6085 1340
Size: 824 1884 2037 1788
Fragments arranged by size:
2037 1884 1788 824
BlpI GC'TnA_GC
Cuts at: 1724 3645 1724
Size: 1921 4612
Bme1580I G_kGCm'C
Cuts at: 664 892 1393 1401 1659 1843 1917 2018 2236
Size: 228 501 8 258 184 74 101 218
Cuts at: 2236 2376 2476 4158 4251 5375 664
Size: 140 100 1682 93 1124 1822
Fragments arranged by size:
1822 1682 1124 501 258 228 218 184
140 101 100 93 74 8
BmgBI CAC'GTC
Cuts at: 723 852 971 2931 723
Size: 129 119 1960 4325
Fragments arranged by size:
4325 1960 129 119
BmrI ACTGGGnnnn_n'
Cuts at: 225 546 1562 2084 2238 2558 2885 2904 3047
Size: 321 1016 522 154 320 327 19 143
Cuts at: 3047 3745 3826 4161 6305 225
Size: 698 81 335 2144 453
Fragments arranged by size:
2144 1016 698 522 453 335 327 321
320 154 143 81 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2363 4900 2363
Size: 2537 3996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 878 1274 498
Size: 380 396 5757
Fragments arranged by size:
5757 396 380
Bpu10I CC'TnA_GC
Cuts at: 133 1826 3175 3476 133
Size: 1693 1349 301 3190
Fragments arranged by size:
3190 1693 1349 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4588 5302 5600 3709
Size: 879 714 298 4642
Fragments arranged by size:
4642 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1066 6237 1066
Size: 5171 1362
BsaAI yAC'GTr
Cuts at: 1110 1652 1706 2252 3886 1110
Size: 542 54 546 1634 3757
Fragments arranged by size:
3757 1634 546 542 54
BsaBI GATnn'nnATC
Cuts at: 6 530 4047 6084 6
Size: 524 3517 2037 455
Fragments arranged by size:
3517 2037 524 455
BsaHI Gr'CG_yC
Cuts at: 2066 3384 3964 4212 4914 6410 2066
Size: 1318 580 248 702 1496 2189
Fragments arranged by size:
2189 1496 1318 702 580 248
BsaWI w'CCGG_w
Cuts at: 757 3722 4243 4921 5332 5479 5753 757
Size: 2965 521 678 411 147 274 1537
Fragments arranged by size:
2965 1537 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1293 1323 1293
Size: 30 6503
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 851 851
Size: 6533
BseYI C'CCAG_C
Cuts at: 494 1835 3958 4716 5381 5789 494
Size: 1341 2123 758 665 408 1238
Fragments arranged by size:
2123 1341 1238 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 534 1632 2115 2135 2316 3018 270
Size: 264 1098 483 20 181 702 3785
Fragments arranged by size:
3785 1098 702 483 264 181 20
BsiEI CG_ry'CG
Cuts at: 104 4075 4121 5351 104
Size: 3971 46 1230 1286
Fragments arranged by size:
3971 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1393 1659 1917 2376 3086 3547 4325 4515 5375
Size: 266 258 459 710 461 778 190 860
Cuts at: 5375 1393
Size: 2551
Fragments arranged by size:
2551 860 778 710 461 459 266 258
190
BsiWI C'GTAC_G
Cuts at: 1706 1706
Size: 6533
BsmI GAATG_Cn'
Cuts at: 739 868 952 739
Size: 129 84 6320
Fragments arranged by size:
6320 129 84
BsmBI CGTCTCn'nnnn_
Cuts at: 3391 3391
Size: 6533
Bsp1286I G_dGCh'C
Cuts at: 582 664 892 1184 1393 1401 1659 1843 1917
Size: 82 228 292 209 8 258 184 74
Cuts at: 1917 2018 2236 2327 2376 2476 3086 3547 4158
Size: 101 218 91 49 100 610 461 611
Cuts at: 4158 4251 4325 4515 4577 5375 582
Size: 93 74 190 62 798 1740
Fragments arranged by size:
1740 798 611 610 461 292 258 228
218 209 190 184 101 100 93 91
82 74 74 62 49 8
BspHI T'CATG_A
Cuts at: 4965 4965
Size: 6533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 721 1084 1654 2137 2229 2542 3452 3583 3797
Size: 363 570 483 92 313 910 131 214
Cuts at: 3797 4099 4480 721
Size: 302 381 2774
Fragments arranged by size:
2774 910 570 483 381 363 313 302
214 131 92
BsrBI CCG'CTC
Cuts at: 50 1115 4825 4879 6363 6528 50
Size: 1065 3710 54 1484 165 55
Fragments arranged by size:
3710 1484 1065 165 55 54
BsrDI GCAATG_nn'
Cuts at: 996 1773 2082 2681 2859 3043 4445 996
Size: 777 309 599 178 184 1402 3084
Fragments arranged by size:
3084 1402 777 599 309 184 178
BsrFI r'CCGG_y
Cuts at: 557 2032 3048 3267 4531 4712 557
Size: 1475 1016 219 1264 181 2378
Fragments arranged by size:
2378 1475 1264 1016 219 181
BssSI C'ACGA_G
Cuts at: 279 4804 5512 279
Size: 4525 708 1300
Fragments arranged by size:
4525 1300 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 2201 3910 1141
Size: 1060 1709 3764
Fragments arranged by size:
3764 1709 1060
BstBI TT'CG_AA
Cuts at: 4894 4894
Size: 6533
BstXI CCAn_nnnn'nTGG
Cuts at: 166 1750 2419 2548 166
Size: 1584 669 129 4151
Fragments arranged by size:
4151 1584 669 129
BstYI r'GATC_y
Cuts at: 1 774 1340 1946 2164 3396 3558 4025 4048
Size: 773 566 606 218 1232 162 467 23
Cuts at: 4048 4383 4629 4935 4947 5033 6085 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1232 1052 773 606 566 467 449 335
306 246 218 162 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1175 1175
Size: 6533
BtgI C'CryG_G
Cuts at: 107 450 1766 107
Size: 343 1316 4874
Fragments arranged by size:
4874 1316 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 539 1957 4483 4664 4827 539
Size: 1418 2526 181 163 2245
Fragments arranged by size:
2526 2245 1418 181 163
BtsI GCAGTG_nn'
Cuts at: 546 2202 2431 3528 3776 546
Size: 1656 229 1097 248 3303
Fragments arranged by size:
3303 1656 1097 248 229
DraI TTT'AAA
Cuts at: 309 3532 309
Size: 3223 3310
DraIII CAC_nnn'GTG
Cuts at: 808 896 1545 2912 808
Size: 88 649 1367 4429
Fragments arranged by size:
4429 1367 649 88
DrdI GACnn_nn'nnGTC
Cuts at: 4239 5583 4239
Size: 1344 5189
EaeI y'GGCC_r
Cuts at: 4118 4292 4683 4710 6055 4118
Size: 174 391 27 1345 4596
Fragments arranged by size:
4596 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4118 4118
Size: 6533
EarI CTCTTCn'nnn_
Cuts at: 2722 3007 3270 4556 4766 2722
Size: 285 263 1286 210 4489
Fragments arranged by size:
4489 1286 285 263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4741 5483 5629 6343 4741
Size: 742 146 714 4931
Fragments arranged by size:
4931 742 714 146
EcoICRI GAG'CTC
Cuts at: 3084 3545 3084
Size: 461 6072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 878 1274 2462 3296 4358 4790 5143 5805
Size: 380 396 1188 834 1062 432 353 662
Cuts at: 5805 498
Size: 1226
Fragments arranged by size:
1226 1188 1062 834 662 432 396 380
353
EcoO109I rG'GnC_Cy
Cuts at: 541 1049 1643 1839 1840 2015 2232 2552 3019
Size: 508 594 196 1 175 217 320 467
Cuts at: 3019 3672 541
Size: 653 3402
Fragments arranged by size:
3402 653 594 508 467 320 217 196
175 1
EcoRI G'AATT_C
Cuts at: 3144 3537 3144
Size: 393 6140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4552 4552
Size: 6533
FspI TGC'GCA
Cuts at: 4314 4314
Size: 6533
HaeII r_GCGC'y
Cuts at: 639 1155 1731 2069 2285 3967 4215 5445 6216
Size: 516 576 338 216 1682 248 1230 771
Cuts at: 6216 639
Size: 956
Fragments arranged by size:
1682 1230 956 771 576 516 338 248
216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 1293 1325 2577 2609 3247 3279 3451
Size: 32 931 32 1252 32 638 32 172
Cuts at: 3451 3483 4375 4405 4407 4437 330
Size: 32 892 30 2 30 2426
Fragments arranged by size:
2426 1252 931 892 638 172 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1089 2934 3572 1089
Size: 1845 638 4050
Fragments arranged by size:
4050 1845 638
HindIII A'AGCT_T
Cuts at: 1328 1328
Size: 6533
KasI G'GCGC_C
Cuts at: 2065 3963 4211 2065
Size: 1898 248 4387
Fragments arranged by size:
4387 1898 248
KpnI G_GTAC'C
Cuts at: 3553 3553
Size: 6533
MluI A'CGCG_T
Cuts at: 5966 5966
Size: 6533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2167 2595 5292 5476 2167
Size: 428 2697 184 3224
Fragments arranged by size:
3224 2697 428 184
MscI TGG'CCA
Cuts at: 4294 4294
Size: 6533
MslI CAynn'nnrTG
Cuts at: 1167 1605 1662 1748 2379 2852 4522 4649 1167
Size: 438 57 86 631 473 1670 127 3051
Fragments arranged by size:
3051 1670 631 473 438 127 86 57
MspA1I CmG'CkG
Cuts at: 650 740 1349 1781 2012 3644 3958 4318 5102
Size: 90 609 432 231 1632 314 360 784
Cuts at: 5102 5347 650
Size: 245 1836
Fragments arranged by size:
1836 1632 784 609 432 360 314 245
231 90
NaeI GCC'GGC
Cuts at: 3050 3269 4714 3050
Size: 219 1445 4869
Fragments arranged by size:
4869 1445 219
NarI GG'CG_CC
Cuts at: 2066 3964 4212 2066
Size: 1898 248 4387
Fragments arranged by size:
4387 1898 248
NcoI C'CATG_G
Cuts at: 107 450 107
Size: 343 6190
NdeI CA'TA_TG
Cuts at: 2487 3195 3288 3402 2487
Size: 708 93 114 5618
Fragments arranged by size:
5618 708 114 93
NgoMIV G'CCGG_C
Cuts at: 3048 3267 4712 3048
Size: 219 1445 4869
Fragments arranged by size:
4869 1445 219
NspI r_CATG'y
Cuts at: 1124 1307 1604 1991 2778 3586 4086 5689 1124
Size: 183 297 387 787 808 500 1603 1968
Fragments arranged by size:
1968 1603 808 787 500 387 297 183
PasI CC'CwG_GG
Cuts at: 3007 3007
Size: 6533
PciI A'CATG_T
Cuts at: 1303 5685 1303
Size: 4382 2151
PfoI T'CCnGG_A
Cuts at: 393 1005 1311 393
Size: 612 306 5615
Fragments arranged by size:
5615 612 306
PmeI GTTT'AAAC
Cuts at: 3532 3532
Size: 6533
PmlI CAC'GTG
Cuts at: 1110 1652 2252 1110
Size: 542 600 5391
Fragments arranged by size:
5391 600 542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2829 2861 4979 5011 2829
Size: 32 2118 32 4351
Fragments arranged by size:
4351 2118 32 32
PpuMI rG'GwC_Cy
Cuts at: 541 1049 1643 2552 541
Size: 508 594 909 4522
Fragments arranged by size:
4522 909 594 508
PspOMI G'GGCC_C
Cuts at: 1839 2014 2232 1839
Size: 175 218 6140
Fragments arranged by size:
6140 218 175
PstI C_TGCA'G
Cuts at: 1348 1741 1981 2281 2566 3580 4265 1348
Size: 393 240 300 285 1014 685 3616
Fragments arranged by size:
3616 1014 685 393 300 285 240
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6533
PvuII CAG'CTG
Cuts at: 1349 3958 4318 1349
Size: 2609 360 3564
Fragments arranged by size:
3564 2609 360
RsrII CG'GwC_CG
Cuts at: 4728 4728
Size: 6533
SacI G_AGCT'C
Cuts at: 3086 3547 3086
Size: 461 6072
SalI G'TCGA_C
Cuts at: 3570 3570
Size: 6533
SanDI GG'GwC_CC
Cuts at: 541 541
Size: 6533
SapI GCTCTTCn'nnn_
Cuts at: 4556 4766 4556
Size: 210 6323
SbfI CC_TGCA'GG
Cuts at: 3580 3580
Size: 6533
ScaI AGT'ACT
Cuts at: 2646 6050 2646
Size: 3404 3129
SfcI C'TryA_G
Cuts at: 33 1344 1737 1977 2277 2562 3344 3576 4261
Size: 1311 393 240 300 285 782 232 685
Cuts at: 4261 5229 5420 33
Size: 968 191 1146
Fragments arranged by size:
1311 1146 968 782 685 393 300 285
240 232 191
SfoI GGC'GCC
Cuts at: 2067 3965 4213 2067
Size: 1898 248 4387
Fragments arranged by size:
4387 1898 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6533
SmaI CCC'GGG
Cuts at: 3555 3555
Size: 6533
SmlI C'TyrA_G
Cuts at: 2631 3688 4603 5317 5579 2631
Size: 1057 915 714 262 3585
Fragments arranged by size:
3585 1057 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6533
SphI G_CATG'C
Cuts at: 1124 2778 3586 4086 1124
Size: 1654 808 500 3571
Fragments arranged by size:
3571 1654 808 500
StyI C'CwwG_G
Cuts at: 107 450 3360 3667 4021 107
Size: 343 2910 307 354 2619
Fragments arranged by size:
2910 2619 354 343 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2923 4116 4249 4708 4917 2923
Size: 1193 133 459 209 4539
Fragments arranged by size:
4539 1193 459 209 133
TatI w'GTAC_w
Cuts at: 1519 2428 2644 3219 6048 1519
Size: 909 216 575 2829 2004
Fragments arranged by size:
2829 2004 909 575 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1907 1939 2097 2129 2181 2213 6050 6082 1907
Size: 32 158 32 52 32 3837 32 2358
Fragments arranged by size:
3837 2358 158 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 721 4330 721
Size: 3609 2924
XbaI T'CTAG_A
Cuts at: 3564 3564
Size: 6533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 571 700 958 2225 4028 571
Size: 129 258 1267 1803 3076
Fragments arranged by size:
3076 1803 1267 258 129
XmaI C'CCGG_G
Cuts at: 3553 3553
Size: 6533
XmnI GAAnn'nnTTC
Cuts at: 3144 3279 3144
Size: 135 6398
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AscI AvrII BaeI BfrBI BmtI
BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI MfeI NheI NotI NruI
NsiI PacI PflMI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI