Restriction map for
pF1KA1776
(Circular) (Six-base) MAPSORT of: pF1KA1776.seq Check: 7574 from: 1 to: 11540
pF1KA1776 11540 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 178 502 1064 1972 2242 2479 2548 2713 4975
Size: 324 562 908 270 237 69 165 2262
Cuts at: 4975 5784 7522 7849 178
Size: 809 1738 327 3869
Fragments arranged by size:
3869 2262 1738 908 809 562 327 324
270 237 165 69
AatII G_ACGT'C
Cuts at: 2921 2921
Size: 11540
AccI GT'mk_AC
Cuts at: 1457 2408 8578 1457
Size: 951 6170 4419
Fragments arranged by size:
6170 4419 951
Acc65I G'GTAC_C
Cuts at: 2509 3106 4259 6796 8556 2509
Size: 597 1153 2537 1760 5493
Fragments arranged by size:
5493 2537 1760 1153 597
AclI AA'CG_TT
Cuts at: 11380 11380
Size: 11540
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2566 2569 8052 8138 8533 9365 9797 10150 10812
Size: 3 5483 86 395 832 432 353 662
Cuts at: 10812 2566
Size: 3294
Fragments arranged by size:
5483 3294 832 662 432 395 353 86
3
AfeI AGC'GCT
Cuts at: 6291 6291
Size: 11540
AflIII A'CryG_T
Cuts at: 237 3811 4850 5086 5591 8892 10692 10973 237
Size: 3574 1039 236 505 3301 1800 281 804
Fragments arranged by size:
3574 3301 1800 1039 804 505 281 236
AgeI A'CCGG_T
Cuts at: 3638 4097 3638
Size: 459 11081
AhdI GACnn_n'nnGTC
Cuts at: 3039 3039
Size: 11540
AleI CACnn'nnGTG
Cuts at: 1487 1951 2278 3977 4895 6512 7226 8372 1487
Size: 464 327 1699 918 1617 714 1146 4655
Fragments arranged by size:
4655 1699 1617 1146 918 714 464 327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3766 5940 3766
Size: 2174 9366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 11508
AlwNI CAG_nnn'CTG
Cuts at: 232 1079 1140 1155 1478 1902 1964 2112 5211
Size: 847 61 15 323 424 62 148 3099
Cuts at: 5211 5637 7436 8845 10283 232
Size: 426 1799 1409 1438 1489
Fragments arranged by size:
3099 1799 1489 1438 1409 847 426 424
323 148 62 61 15
ApaI G_GGCC'C
Cuts at: 142 599 1291 7004 7109 7712 7800 142
Size: 457 692 5713 105 603 88 3882
Fragments arranged by size:
5713 3882 692 603 457 105 88
ApaLI G'TGCA_C
Cuts at: 408 720 3610 5478 6675 10378 408
Size: 312 2890 1868 1197 3703 1570
Fragments arranged by size:
3703 2890 1868 1570 1197 312
ApoI r'AATT_y
Cuts at: 1129 8544 10893 11149 1129
Size: 7415 2349 256 1520
Fragments arranged by size:
7415 2349 1520 256
AscI GG'CGCG_CC
Cuts at: 369 369
Size: 11540
AseI AT'TA_AT
Cuts at: 20 20
Size: 11540
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11540
AvaI C'yCGr_G
Cuts at: 685 1115 1533 2620 2675 2736 2977 4568 4652
Size: 430 418 1087 55 61 241 1591 84
Cuts at: 4652 4746 5229 5483 5950 6350 6767 7403 7623
Size: 94 483 254 467 400 417 636 220
Cuts at: 7623 7921 8156 8560 685
Size: 298 235 404 3665
Fragments arranged by size:
3665 1591 1087 636 483 467 430 418
417 404 400 298 254 241 235 220
94 84 61 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4251 4284 4251
Size: 33 11507
BamHI G'GATC_C
Cuts at: 1207 1274 2172 2973 6421 8315 8565 1207
Size: 67 898 801 3448 1894 250 4182
Fragments arranged by size:
4182 3448 1894 898 801 250 67
BanI G'GyrC_C
Cuts at: 490 535 692 985 1510 1797 2254 2324 2509
Size: 45 157 293 525 287 457 70 185
Cuts at: 2509 2536 3106 3232 3508 4028 4259 4963 6055
Size: 27 570 126 276 520 231 704 1092
Cuts at: 6055 6125 6189 6796 7135 7504 7597 8556 8970
Size: 70 64 607 339 369 93 959 414
Cuts at: 8970 9218 9253 490
Size: 248 35 2777
Fragments arranged by size:
2777 1092 959 704 607 570 525 520
457 414 369 339 293 287 276 248
231 185 157 126 93 70 70 64
45 35 27
BanII G_rGCy'C
Cuts at: 142 599 913 1123 1291 1673 1885 1990 2069
Size: 457 314 210 168 382 212 105 79
Cuts at: 2069 2641 5867 5959 6038 6308 6667 7004 7109
Size: 572 3226 92 79 270 359 337 105
Cuts at: 7109 7121 7712 7800 8285 8554 9584 142
Size: 12 591 88 485 269 1030 2098
Fragments arranged by size:
3226 2098 1030 591 572 485 457 382
359 337 314 270 269 212 210 168
105 105 92 88 79 79 12
BbeI G_GCGC'C
Cuts at: 989 1801 7508 8974 9222 989
Size: 812 5707 1466 248 3307
Fragments arranged by size:
5707 3307 1466 812 248
BbsI GAAGACnn'nnnn_
Cuts at: 977 4196 4799 6362 977
Size: 3219 603 1563 6155
Fragments arranged by size:
6155 3219 1563 603
BbvCI CC'TCA_GC
Cuts at: 5908 7184 5908
Size: 1276 10264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2388 2422 3174 3208 4643 4677 5997 6031 2388
Size: 34 752 34 1435 34 1320 34 7897
Fragments arranged by size:
7897 1435 1320 752 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9438 10494 11540 9438
Size: 1056 1046 9438
Fragments arranged by size:
9438 1056 1046
BclI T'GATC_A
Cuts at: 7911 9060 7911
Size: 1149 10391
BfrBI ATG'CAT
Cuts at: 4082 8364 4082
Size: 4282 7258
BglI GCCn_nnn'nGGC
Cuts at: 169 220 680 809 887 1178 1199 1557 1682
Size: 51 460 129 78 291 21 358 125
Cuts at: 1682 2366 3359 3533 4037 4883 5357 5807 5855
Size: 684 993 174 504 846 474 450 48
Cuts at: 5855 5933 6053 6101 6317 7166 7652 7727 7898
Size: 78 120 48 216 849 486 75 171
Cuts at: 7898 8478 169
Size: 580 3231
Fragments arranged by size:
3231 993 849 846 684 580 504 486
474 460 450 358 291 216 174 171
129 125 120 78 78 75 51 48
48 21
BglII A'GATC_T
Cuts at: 2393 9055 11092 2393
Size: 6662 2037 2841
Fragments arranged by size:
6662 2841 2037
BlpI GC'TnA_GC
Cuts at: 1672 6881 7575 8652 1672
Size: 5209 694 1077 4560
Fragments arranged by size:
5209 4560 1077 694
Bme1580I G_kGCm'C
Cuts at: 142 412 493 599 724 1291 1998 2295 2539
Size: 270 81 106 125 567 707 297 244
Cuts at: 2539 2676 3007 3235 3433 3614 4711 4747 4879
Size: 137 331 228 198 181 1097 36 132
Cuts at: 4879 5353 5482 6128 6194 6679 7004 7109 7162
Size: 474 129 646 66 485 325 105 53
Cuts at: 7162 7602 7610 7712 7800 7827 8048 8181 9165
Size: 440 8 102 88 27 221 133 984
Cuts at: 9165 9258 10382 142
Size: 93 1124 1300
Fragments arranged by size:
1300 1124 1097 984 707 646 567 485
474 440 331 325 297 270 244 228
221 198 181 137 133 132 129 125
106 105 102 93 88 81 66 53
36 27 8
BmgBI CAC'GTC
Cuts at: 238 1434 2280 3321 3609 3696 6360 238
Size: 1196 846 1041 288 87 2664 5418
Fragments arranged by size:
5418 2664 1196 1041 846 288 87
BmrI ACTGGGnnnn_n'
Cuts at: 4456 7785 7794 8752 8833 9168 11312 4456
Size: 3329 9 958 81 335 2144 4684
Fragments arranged by size:
4684 3329 2144 958 335 81 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2004 8127 9907 2004
Size: 6123 1780 3637
Fragments arranged by size:
6123 3637 1780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 342 1043 3007 4609 7438 8299 8317 8452
Size: 206 701 1964 1602 2829 861 18 135
Cuts at: 8452 136
Size: 3224
Fragments arranged by size:
3224 2829 1964 1602 861 701 206 135
18
Bpu10I CC'TnA_GC
Cuts at: 1100 5908 7170 7184 7671 1100
Size: 4808 1262 14 487 4969
Fragments arranged by size:
4969 4808 1262 487 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 8003 8526 8716 9595 10309 10607 8003
Size: 523 190 879 714 298 8936
Fragments arranged by size:
8936 879 714 523 298 190
BsaI GGTCTCn'nnnn_
Cuts at: 1019 6251 7043 11244 1019
Size: 5232 792 4201 1315
Fragments arranged by size:
5232 4201 1315 792
BsaAI yAC'GTr
Cuts at: 1953 8893 1953
Size: 6940 4600
BsaBI GATnn'nnATC
Cuts at: 6 9054 11091 6
Size: 9048 2037 455
Fragments arranged by size:
9048 2037 455
BsaHI Gr'CG_yC
Cuts at: 505 986 1798 1840 2441 2816 2918 6386 6401
Size: 481 812 42 601 375 102 3468 15
Cuts at: 6401 7505 8378 8971 9219 9921 11417 505
Size: 1104 873 593 248 702 1496 628
Fragments arranged by size:
3468 1496 1104 873 812 702 628 601
593 481 375 248 102 42 15
BsaWI w'CCGG_w
Cuts at: 653 2046 2909 3638 4097 6932 7293 8729 9250
Size: 1393 863 729 459 2835 361 1436 521
Cuts at: 9250 9928 10339 10486 10760 653
Size: 678 411 147 274 1433
Fragments arranged by size:
2835 1436 1433 1393 863 729 678 521
459 411 361 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 109 139 333 363 417 447 2338 2368 4084
Size: 30 194 30 54 30 1891 30 1716
Cuts at: 4084 4114 4631 4661 4918 4948 6601 6631 8038
Size: 30 517 30 257 30 1653 30 1407
Cuts at: 8038 8068 109
Size: 30 3581
Fragments arranged by size:
3581 1891 1716 1653 1407 517 257 194
54 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1103 6046 7435 8116 1103
Size: 4943 1389 681 4527
Fragments arranged by size:
4943 4527 1389 681
BseYI C'CCAG_C
Cuts at: 203 395 436 1079 1148 1279 2773 3015 3906
Size: 192 41 643 69 131 1494 242 891
Cuts at: 3906 4040 4543 6175 6251 6961 7100 7538 7580
Size: 134 503 1632 76 710 139 438 42
Cuts at: 7580 7781 8035 8394 8965 9723 10388 10796 203
Size: 201 254 359 571 758 665 408 947
Fragments arranged by size:
1632 1494 947 891 758 710 665 643
571 503 438 408 359 254 242 201
192 139 134 131 76 69 42 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 770 2136 2256 3081 3168 3663 3710 3726
Size: 284 1366 120 825 87 495 47 16
Cuts at: 3726 4122 4131 4419 4475 6013 6375 6392 6761
Size: 396 9 288 56 1538 362 17 369
Cuts at: 6761 6840 7353 7413 8539 486
Size: 79 513 60 1126 3487
Fragments arranged by size:
3487 1538 1366 1126 825 513 495 396
369 362 288 284 120 87 79 60
56 47 17 16 9
BsiEI CG_ry'CG
Cuts at: 104 6906 8290 8410 9082 9128 10358 104
Size: 6802 1384 120 672 46 1230 1286
Fragments arranged by size:
6802 1384 1286 1230 672 120 46
BsiHKAI G_wGCw'C
Cuts at: 412 724 1673 2169 3460 3523 3547 3614 4097
Size: 312 949 496 1291 63 24 67 483
Cuts at: 4097 4904 5482 5867 6028 6187 6308 6499 6679
Size: 807 578 385 161 159 121 191 180
Cuts at: 6679 7121 7233 7576 8285 8554 9332 9522 10382
Size: 442 112 343 709 269 778 190 860
Cuts at: 10382 412
Size: 1570
Fragments arranged by size:
1570 1291 949 860 807 778 709 578
496 483 442 385 343 312 269 191
190 180 161 159 121 112 67 63
24
BsmI GAATG_Cn'
Cuts at: 1468 2419 3079 3586 3961 4084 4936 5442 6619
Size: 951 660 507 375 123 852 506 1177
Cuts at: 6619 6877 7210 7459 7648 8137 1468
Size: 258 333 249 189 489 4871
Fragments arranged by size:
4871 1177 951 852 660 507 506 489
375 333 258 249 189 123
BsmBI CGTCTCn'nnnn_
Cuts at: 4585 4585
Size: 11540
Bsp1286I G_dGCh'C
Cuts at: 142 412 493 599 724 913 1123 1291 1673
Size: 270 81 106 125 189 210 168 382
Cuts at: 1673 1885 1990 1998 2069 2169 2295 2539 2641
Size: 212 105 8 71 100 126 244 102
Cuts at: 2641 2676 3007 3235 3433 3460 3523 3547 3614
Size: 35 331 228 198 27 63 24 67
Cuts at: 3614 4097 4711 4747 4879 4904 5353 5482 5867
Size: 483 614 36 132 25 449 129 385
Cuts at: 5867 5959 6028 6038 6128 6187 6194 6308 6499
Size: 92 69 10 90 59 7 114 191
Cuts at: 6499 6667 6679 7004 7109 7121 7162 7233 7576
Size: 168 12 325 105 12 41 71 343
Cuts at: 7576 7602 7610 7712 7800 7827 8048 8181 8285
Size: 26 8 102 88 27 221 133 104
Cuts at: 8285 8554 9165 9258 9332 9522 9584 10382 142
Size: 269 611 93 74 190 62 798 1300
Fragments arranged by size:
1300 798 614 611 483 449 385 382
343 331 325 270 269 244 228 221
212 210 198 191 190 189 168 168
133 132 129 126 125 114 106 105
105 104 102 102 100 93 92 90
88 81 74 71 71 69 67 63
62 59 41 36 35 27 27 26
25 24 12 12 10 8 8 7
BspEI T'CCGG_A
Cuts at: 2046 2046
Size: 11540
BspHI T'CATG_A
Cuts at: 6587 9972 6587
Size: 3385 8155
BspMI ACCTGCnnnn'nnnn_
Cuts at: 178 217 502 1064 1972 2242 2479 2548 2713
Size: 39 285 562 908 270 237 69 165
Cuts at: 2713 3118 4379 4975 5695 5784 5932 6604 7321
Size: 405 1261 596 720 89 148 672 717
Cuts at: 7321 7421 7444 7522 7849 8590 8804 9106 9487
Size: 100 23 78 327 741 214 302 381
Cuts at: 9487 178
Size: 2231
Fragments arranged by size:
2231 1261 908 741 720 717 672 596
562 405 381 327 302 285 270 237
214 165 148 100 89 78 69 39
23
BsrBI CCG'CTC
Cuts at: 50 9832 9886 11370 11535 50
Size: 9782 54 1484 165 55
Fragments arranged by size:
9782 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 918 1662 4404 5811 9452 918
Size: 744 2742 1407 3641 3006
Fragments arranged by size:
3641 3006 2742 1407 744
BsrFI r'CCGG_y
Cuts at: 941 982 1033 1523 2458 2492 2825 3532 3638
Size: 41 51 490 935 34 333 707 106
Cuts at: 3638 4097 5663 6688 7034 8162 8289 8426 9538
Size: 459 1566 1025 346 1128 127 137 1112
Cuts at: 9538 9719 941
Size: 181 2762
Fragments arranged by size:
2762 1566 1128 1112 1025 935 707 490
459 346 333 181 137 127 106 51
41 34
BssHII G'CGCG_C
Cuts at: 369 369
Size: 11540
BssSI C'ACGA_G
Cuts at: 715 9811 10519 715
Size: 9096 708 1736
Fragments arranged by size:
9096 1736 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1055 1478 1727 1964 2270 4622 5039 6779 7436
Size: 423 249 237 306 2352 417 1740 657
Cuts at: 7436 8917 1055
Size: 1481 3678
Fragments arranged by size:
3678 2352 1740 1481 657 423 417 306
249 237
BstBI TT'CG_AA
Cuts at: 4058 6551 9901 4058
Size: 2493 3350 5697
Fragments arranged by size:
5697 3350 2493
BstEII G'GTnAC_C
Cuts at: 2889 3760 2889
Size: 871 10669
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1218 2059 3598 4551 5026 6514 8492 114
Size: 1104 841 1539 953 475 1488 1978 3162
Fragments arranged by size:
3162 1978 1539 1488 1104 953 841 475
BstYI r'GATC_y
Cuts at: 1 1207 1274 2172 2393 2552 2973 5207 5288
Size: 1206 67 898 221 159 421 2234 81
Cuts at: 5288 5540 6421 8315 8565 9032 9055 9390 9636
Size: 252 881 1894 250 467 23 335 246
Cuts at: 9636 9942 9954 10040 11092 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2234 1894 1206 1052 898 881 467 449
421 335 306 252 250 246 221 159
86 81 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 2177 2259 2820 2177
Size: 82 561 10897
Fragments arranged by size:
10897 561 82
BtgI C'CryG_G
Cuts at: 564 807 1254 1485 2052 2138 3007 3483 3858
Size: 243 447 231 567 86 869 476 375
Cuts at: 3858 5559 5607 6321 6920 7164 7602 7677 8334
Size: 1701 48 714 599 244 438 75 657
Cuts at: 8334 8373 8491 564
Size: 39 118 3613
Fragments arranged by size:
3613 1701 869 714 657 599 567 476
447 438 375 244 243 231 118 86
75 48 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2723 2880 9490 9671 9834 2723
Size: 157 6610 181 163 4429
Fragments arranged by size:
6610 4429 181 163 157
BtsI GCAGTG_nn'
Cuts at: 474 1060 1310 1518 2127 2768 3135 3431 3738
Size: 586 250 208 609 641 367 296 307
Cuts at: 3738 4243 4632 5838 6003 7059 7178 7608 8181
Size: 505 389 1206 165 1056 119 430 573
Cuts at: 8181 8783 474
Size: 602 3231
Fragments arranged by size:
3231 1206 1056 641 609 602 586 573
505 430 389 367 307 296 250 208
165 119
ClaI AT'CG_AT
Cuts at: 3449 3449
Size: 11540
DraI TTT'AAA
Cuts at: 8539 8539
Size: 11540
DraIII CAC_nnn'GTG
Cuts at: 542 1727 8196 542
Size: 1185 6469 3886
Fragments arranged by size:
6469 3886 1185
DrdI GACnn_nn'nnGTC
Cuts at: 6895 9246 10590 6895
Size: 2351 1344 7845
Fragments arranged by size:
7845 2351 1344
EaeI y'GGCC_r
Cuts at: 192 672 1035 1170 1371 1806 1860 1929 2456
Size: 480 363 135 201 435 54 69 527
Cuts at: 2456 3045 3225 3422 3486 3791 4715 5148 5808
Size: 589 180 197 64 305 924 433 660
Cuts at: 5808 6318 6504 6903 7092 8164 8204 8287 8407
Size: 510 186 399 189 1072 40 83 120
Cuts at: 8407 9125 9299 9690 9717 11062 192
Size: 718 174 391 27 1345 670
Fragments arranged by size:
1345 1072 924 718 670 660 589 527
510 480 435 433 399 391 363 305
201 197 189 186 180 174 135 120
83 69 64 54 40 27
EagI C'GGCC_G
Cuts at: 6903 8287 8407 9125 6903
Size: 1384 120 718 9318
Fragments arranged by size:
9318 1384 718 120
EarI CTCTTCn'nnn_
Cuts at: 1189 3704 5050 9563 9773 1189
Size: 2515 1346 4513 210 2956
Fragments arranged by size:
4513 2956 2515 1346 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8415 9748 10490 10636 11350 8415
Size: 1333 742 146 714 8605
Fragments arranged by size:
8605 1333 742 714 146
EcoICRI GAG'CTC
Cuts at: 1671 5865 6306 7119 8283 8552 1671
Size: 4194 441 813 1164 269 4659
Fragments arranged by size:
4659 4194 1164 813 441 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 342 1043 2566 2569 3007 4609 7438 8052
Size: 206 701 1523 3 438 1602 2829 614
Cuts at: 8052 8138 8299 8317 8452 8533 9365 9797 10150
Size: 86 161 18 135 81 832 432 353
Cuts at: 10150 10812 136
Size: 662 864
Fragments arranged by size:
2829 1602 1523 864 832 701 662 614
438 432 353 206 161 135 86 81
18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 880 5848 6703 7417 880
Size: 4968 855 714 5003
Fragments arranged by size:
5003 4968 855 714
EcoO109I rG'GnC_Cy
Cuts at: 138 139 212 229 631 661 682 762 836
Size: 1 73 17 402 30 21 80 74
Cuts at: 836 1222 1288 2802 3339 5226 5684 7000 7001
Size: 386 66 1514 537 1887 458 1316 1
Cuts at: 7001 7105 7106 7341 7433 7709 7917 8223 8413
Size: 104 1 235 92 276 208 306 190
Cuts at: 8413 8455 8679 138
Size: 42 224 2999
Fragments arranged by size:
2999 1887 1514 1316 537 458 402 386
306 276 235 224 208 190 104 92
80 74 73 66 42 30 21 17
1 1 1
EcoRI G'AATT_C
Cuts at: 1129 8544 1129
Size: 7415 4125
EcoRV GAT'ATC
Cuts at: 3192 6015 3192
Size: 2823 8717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3903 9559 3903
Size: 5656 5884
FseI GG_CCGG'CC
Cuts at: 8293 8293
Size: 11540
FspI TGC'GCA
Cuts at: 1957 4334 9321 1957
Size: 2377 4987 4176
Fragments arranged by size:
4987 4176 2377
HaeII r_GCGC'y
Cuts at: 962 989 1801 6293 7508 7853 8974 9222 10452
Size: 27 812 4492 1215 345 1121 248 1230
Cuts at: 10452 11223 962
Size: 771 1279
Fragments arranged by size:
4492 1279 1230 1215 1121 812 771 345
248 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 417 449 929 961 2713 2745 2965 2997 3025
Size: 32 480 32 1752 32 220 32 28
Cuts at: 3025 3057 3442 3474 4631 4663 4918 4950 6010
Size: 32 385 32 1157 32 255 32 1060
Cuts at: 6010 6042 6481 6513 6601 6633 7558 7590 9382
Size: 32 439 32 88 32 925 32 1792
Cuts at: 9382 9412 9414 9444 417
Size: 30 2 30 2513
Fragments arranged by size:
2513 1792 1752 1157 1060 925 480 439
385 255 220 88 32 32 32 32
32 32 32 32 32 32 32 32
30 30 28 2
HincII GTy'rAC
Cuts at: 900 1500 1626 1734 2409 2766 3243 4848 6411
Size: 600 126 108 675 357 477 1605 1563
Cuts at: 6411 6900 6915 7494 8579 900
Size: 489 15 579 1085 3861
Fragments arranged by size:
3861 1605 1563 1085 675 600 579 489
477 357 126 108 15
HindIII A'AGCT_T
Cuts at: 6197 6197
Size: 11540
KasI G'GCGC_C
Cuts at: 985 1797 7504 8970 9218 985
Size: 812 5707 1466 248 3307
Fragments arranged by size:
5707 3307 1466 812 248
KpnI G_GTAC'C
Cuts at: 2513 3110 4263 6800 8560 2513
Size: 597 1153 2537 1760 5493
Fragments arranged by size:
5493 2537 1760 1153 597
MluI A'CGCG_T
Cuts at: 10973 10973
Size: 11540
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 915 2428 3945 10299 10483 915
Size: 1513 1517 6354 184 1972
Fragments arranged by size:
6354 1972 1517 1513 184
MscI TGG'CCA
Cuts at: 194 674 3488 3793 4717 5150 5810 6320 8206
Size: 480 2814 305 924 433 660 510 1886
Cuts at: 8206 9301 194
Size: 1095 2433
Fragments arranged by size:
2814 2433 1886 1095 924 660 510 480
433 305
MslI CAynn'nnrTG
Cuts at: 1487 1852 1951 2278 3301 3319 3748 3977 4895
Size: 365 99 327 1023 18 429 229 918
Cuts at: 4895 5047 6512 7226 7682 8372 9529 9656 1487
Size: 152 1465 714 456 690 1157 127 3371
Fragments arranged by size:
3371 1465 1157 1023 918 714 690 456
429 365 327 229 152 127 99 18
MspA1I CmG'CkG
Cuts at: 203 440 566 605 705 809 893 926 1016
Size: 237 126 39 100 104 84 33 90
Cuts at: 1016 1087 1152 1161 1376 1403 1619 1892 2098
Size: 71 65 9 215 27 216 273 206
Cuts at: 2098 2225 2351 2567 2786 3009 3345 3647 3767
Size: 127 126 216 219 223 336 302 120
Cuts at: 3767 3906 4022 4145 4265 4388 4517 4547 5342
Size: 139 116 123 120 123 129 30 795
Cuts at: 5342 5471 5827 5872 5966 6221 6431 6630 6647
Size: 129 356 45 94 255 210 199 17
Cuts at: 6647 6908 6922 7112 7268 7397 7520 7637 7718
Size: 261 14 190 156 129 123 117 81
Cuts at: 7718 7757 7763 7883 7979 8336 8394 8523 8651
Size: 39 6 120 96 357 58 129 128
Cuts at: 8651 8965 9325 10109 10354 203
Size: 314 360 784 245 1389
Fragments arranged by size:
1389 795 784 360 357 356 336 314
302 273 261 255 245 237 223 219
216 216 215 210 206 199 190 156
139 129 129 129 129 128 127 126
126 123 123 123 120 120 120 117
116 104 100 96 94 90 84 81
71 65 58 45 39 39 33 30
27 17 14 9 6
NaeI GCC'GGC
Cuts at: 984 1035 2460 2494 2827 3534 6690 8291 9721
Size: 51 1425 34 333 707 3156 1601 1430
Cuts at: 9721 984
Size: 2803
Fragments arranged by size:
3156 2803 1601 1430 1425 707 333 51
34
NarI GG'CG_CC
Cuts at: 986 1798 7505 8971 9219 986
Size: 812 5707 1466 248 3307
Fragments arranged by size:
5707 3307 1466 812 248
NcoI C'CATG_G
Cuts at: 1254 3483 3858 5559 7164 7677 8491 1254
Size: 2229 375 1701 1605 513 814 4303
Fragments arranged by size:
4303 2229 1701 1605 814 513 375
NgoMIV G'CCGG_C
Cuts at: 982 1033 2458 2492 2825 3532 6688 8289 9719
Size: 51 1425 34 333 707 3156 1601 1430
Cuts at: 9719 982
Size: 2803
Fragments arranged by size:
3156 2803 1601 1430 1425 707 333 51
34
NsiI A_TGCA'T
Cuts at: 4084 8366 4084
Size: 4282 7258
NspI r_CATG'y
Cuts at: 968 1568 3689 3753 3815 5090 5595 6530 6828
Size: 600 2121 64 62 1275 505 935 298
Cuts at: 6828 8364 8593 9093 10696 968
Size: 1536 229 500 1603 1812
Fragments arranged by size:
2121 1812 1603 1536 1275 935 600 505
500 298 229 64 62
PasI CC'CwG_GG
Cuts at: 3406 4009 4674 4980 6073 7770 8459 3406
Size: 603 665 306 1093 1697 689 6487
Fragments arranged by size:
6487 1697 1093 689 665 603 306
PciI A'CATG_T
Cuts at: 3811 5086 5591 10692 3811
Size: 1275 505 5101 4659
Fragments arranged by size:
5101 4659 1275 505
PflMI CCAn_nnn'nTGG
Cuts at: 849 1902 4320 7806 7938 849
Size: 1053 2418 3486 132 4451
Fragments arranged by size:
4451 3486 2418 1053 132
PfoI T'CCnGG_A
Cuts at: 231 2555 4586 8276 231
Size: 2324 2031 3690 3495
Fragments arranged by size:
3690 3495 2324 2031
PmeI GTTT'AAAC
Cuts at: 8539 8539
Size: 11540
PmlI CAC'GTG
Cuts at: 1953 1953
Size: 11540
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9986 10018 9986
Size: 32 11508
PpuMI rG'GwC_Cy
Cuts at: 229 631 661 1222 3339 5684 7341 7433 8455
Size: 402 30 561 2117 2345 1657 92 1022
Cuts at: 8455 229
Size: 3314
Fragments arranged by size:
3314 2345 2117 1657 1022 561 402 92
30
PshAI GACnn'nnGTC
Cuts at: 2698 3200 2698
Size: 502 11038
PspOMI G'GGCC_C
Cuts at: 138 595 1287 7000 7105 7708 7796 138
Size: 457 692 5713 105 603 88 3882
Fragments arranged by size:
5713 3882 692 603 457 105 88
PspXI vC'TCGA_Gb
Cuts at: 5950 5950
Size: 11540
PstI C_TGCA'G
Cuts at: 228 3115 4516 4627 5010 5628 6232 7025 7762
Size: 2887 1401 111 383 618 604 793 737
Cuts at: 7762 8393 8528 8587 9272 228
Size: 631 135 59 685 2496
Fragments arranged by size:
2887 2496 1401 793 737 685 631 618
604 383 135 111 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11540
PvuII CAG'CTG
Cuts at: 440 705 893 1087 2351 3647 3906 4022 4517
Size: 265 188 194 1264 1296 259 116 495
Cuts at: 4517 5827 5872 7397 7763 7979 8394 8523 8965
Size: 1310 45 1525 366 216 415 129 442
Cuts at: 8965 9325 440
Size: 360 2655
Fragments arranged by size:
2655 1525 1310 1296 1264 495 442 415
366 360 265 259 216 194 188 129
116 45
RsrII CG'GwC_CG
Cuts at: 9735 9735
Size: 11540
SacI G_AGCT'C
Cuts at: 1673 5867 6308 7121 8285 8554 1673
Size: 4194 441 813 1164 269 4659
Fragments arranged by size:
4659 4194 1164 813 441 269
SacII CC_GC'GG
Cuts at: 567 810 3010 6923 8337 567
Size: 243 2200 3913 1414 3770
Fragments arranged by size:
3913 3770 2200 1414 243
SalI G'TCGA_C
Cuts at: 2407 8577 2407
Size: 6170 5370
SanDI GG'GwC_CC
Cuts at: 661 1222 3339 661
Size: 561 2117 8862
Fragments arranged by size:
8862 2117 561
SapI GCTCTTCn'nnn_
Cuts at: 9563 9773 9563
Size: 210 11330
SbfI CC_TGCA'GG
Cuts at: 8587 8587
Size: 11540
ScaI AGT'ACT
Cuts at: 11057 11057
Size: 11540
SexAI A'CCwGG_T
Cuts at: 8192 8192
Size: 11540
SfcI C'TryA_G
Cuts at: 33 224 363 2346 3111 4023 4512 4623 5006
Size: 191 139 1983 765 912 489 111 383
Cuts at: 5006 5553 5624 6228 6450 7021 7146 7758 7968
Size: 547 71 604 222 571 125 612 210
Cuts at: 7968 7974 8389 8524 8583 9268 10236 10427 33
Size: 6 415 135 59 685 968 191 1146
Fragments arranged by size:
1983 1146 968 912 765 685 612 604
571 547 489 415 383 222 210 191
191 139 135 125 111 71 59 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 169 680 1178 169
Size: 511 498 10531
Fragments arranged by size:
10531 511 498
SfoI GGC'GCC
Cuts at: 987 1799 7506 8972 9220 987
Size: 812 5707 1466 248 3307
Fragments arranged by size:
5707 3307 1466 812 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11540
SgrAI Cr'CCGG_yG
Cuts at: 4097 4097
Size: 11540
SmaI CCC'GGG
Cuts at: 1535 2677 2738 4748 5231 6769 7625 8562 1535
Size: 1142 61 2010 483 1538 856 937 4513
Fragments arranged by size:
4513 2010 1538 1142 937 856 483 61
SmlI C'TyrA_G
Cuts at: 5950 8018 8505 8695 9610 10324 10586 5950
Size: 2068 487 190 915 714 262 6904
Fragments arranged by size:
6904 2068 915 714 487 262 190
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11540
SphI G_CATG'C
Cuts at: 1568 6828 8364 8593 9093 1568
Size: 5260 1536 229 500 4015
Fragments arranged by size:
5260 4015 1536 500 229
SspI AAT'ATT
Cuts at: 1305 1305
Size: 11540
StuI AGG'CCT
Cuts at: 875 2965 5155 5176 5620 6240 7552 8503 875
Size: 2090 2190 21 444 620 1312 951 3912
Fragments arranged by size:
3912 2190 2090 1312 951 620 444 21
StyI C'CwwG_G
Cuts at: 195 215 725 1254 2531 3425 3483 3732 3858
Size: 20 510 529 1277 894 58 249 126
Cuts at: 3858 5559 7164 7254 7650 7677 8234 8491 8674
Size: 1701 1605 90 396 27 557 257 183
Cuts at: 8674 9028 195
Size: 354 2707
Fragments arranged by size:
2707 1701 1605 1277 894 557 529 510
396 354 257 249 183 126 90 58
27 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1441 1963 3590 4032 4433 4616 7552 7795 9123
Size: 522 1627 442 401 183 2936 243 1328
Cuts at: 9123 9256 9715 9924 1441
Size: 133 459 209 3057
Fragments arranged by size:
3057 2936 1627 1328 522 459 442 401
243 209 183 133
TatI w'GTAC_w
Cuts at: 4730 5776 11055 4730
Size: 1046 5279 5215
Fragments arranged by size:
5279 5215 1046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 333 365 2682 2714 4084 4116 4332 4364 5871
Size: 32 2317 32 1370 32 216 32 1507
Cuts at: 5871 5903 8038 8070 11057 11089 333
Size: 32 2135 32 2987 32 784
Fragments arranged by size:
2987 2317 2135 1507 1370 784 216 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1495 4843 9337 1495
Size: 3348 4494 3698
Fragments arranged by size:
4494 3698 3348
XbaI T'CTAG_A
Cuts at: 2520 8304 8571 2520
Size: 5784 267 5489
Fragments arranged by size:
5784 5489 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 3403 5026 5212 9035 2335
Size: 1068 1623 186 3823 4840
Fragments arranged by size:
4840 3823 1623 1068 186
XhoI C'TCGA_G
Cuts at: 5950 5950
Size: 11540
XmaI C'CCGG_G
Cuts at: 1533 2675 2736 4746 5229 6767 7623 8560 1533
Size: 1142 61 2010 483 1538 856 937 4513
Fragments arranged by size:
4513 2010 1538 1142 937 856 483 61
XmnI GAAnn'nnTTC
Cuts at: 4582 4582
Size: 11540
ZraI GAC'GTC
Cuts at: 2919 2919
Size: 11540
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AvrII BmtI BsiWI BsrGI BstZ17I CspCI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PsiI
PsrI SnaBI SrfI SwaI