Restriction map for
pF1KA1747
(Circular) (Six-base) MAPSORT of: pF1KA1747.seq Check: 6457 from: 1 to: 5462
pF1KA1747 5462 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1447 1447
Size: 5462
AccI GT'mk_AC
Cuts at: 2500 2500
Size: 5462
Acc65I G'GTAC_C
Cuts at: 338 2478 338
Size: 2140 3322
AclI AA'CG_TT
Cuts at: 5302 5302
Size: 5462
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 871 1195 1789 3287 3719 4466 4734 414
Size: 457 324 594 1498 432 747 268 1142
Fragments arranged by size:
1498 1142 747 594 457 432 324 268
AfeI AGC'GCT
Cuts at: 919 1303 919
Size: 384 5078
AflII C'TTAA_G
Cuts at: 2353 2353
Size: 5462
AflIII A'CryG_T
Cuts at: 2814 3918 4895 2814
Size: 1104 977 3381
Fragments arranged by size:
3381 1104 977
AgeI A'CCGG_T
Cuts at: 793 1157 3850 793
Size: 364 2693 2405
Fragments arranged by size:
2693 2405 364
AleI CACnn'nnGTG
Cuts at: 670 670
Size: 5462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1729 1729
Size: 5462
AlwNI CAG_nnn'CTG
Cuts at: 606 894 1134 1230 1575 1641 2767 4334 606
Size: 288 240 96 345 66 1126 1567 1734
Fragments arranged by size:
1734 1567 1126 345 288 240 96 66
ApaI G_GGCC'C
Cuts at: 298 1471 1499 298
Size: 1173 28 4261
Fragments arranged by size:
4261 1173 28
ApaLI G'TGCA_C
Cuts at: 1798 4232 1798
Size: 2434 3028
ApoI r'AATT_y
Cuts at: 2127 2466 4815 5071 2127
Size: 339 2349 256 2518
Fragments arranged by size:
2518 2349 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5462
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
AvaI C'yCGr_G
Cuts at: 235 1551 2482 235
Size: 1316 931 3215
Fragments arranged by size:
3215 1316 931
BamHI G'GATC_C
Cuts at: 1271 2381 2487 1271
Size: 1110 106 4246
Fragments arranged by size:
4246 1110 106
BanI G'GyrC_C
Cuts at: 338 634 2478 2892 3140 3175 338
Size: 296 1844 414 248 35 2625
Fragments arranged by size:
2625 1844 414 296 248 35
BanII G_rGCy'C
Cuts at: 298 971 1471 1499 2102 2276 2476 3506 298
Size: 673 500 28 603 174 200 1030 2254
Fragments arranged by size:
2254 1030 673 603 500 200 174 28
BbeI G_GCGC'C
Cuts at: 2896 3144 2896
Size: 248 5214
BbsI GAAGACnn'nnnn_
Cuts at: 2020 2020
Size: 5462
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 226 260 226
Size: 34 5428
BciVI GTATCCnnnnn_n'
Cuts at: 3360 4121 5462 3360
Size: 761 1341 3360
Fragments arranged by size:
3360 1341 761
BclI T'GATC_A
Cuts at: 2982 2982
Size: 5462
BglI GCCn_nnn'nGGC
Cuts at: 175 191 220 766 175
Size: 16 29 546 4871
Fragments arranged by size:
4871 546 29 16
BglII A'GATC_T
Cuts at: 1826 2977 5014 1826
Size: 1151 2037 2274
Fragments arranged by size:
2274 2037 1151
BlpI GC'TnA_GC
Cuts at: 2574 2574
Size: 5462
Bme1580I G_kGCm'C
Cuts at: 298 1471 1499 1509 1802 3087 3180 4236 298
Size: 1173 28 10 293 1285 93 1056 1524
Fragments arranged by size:
1524 1285 1173 1056 293 93 28 10
BmrI ACTGGGnnnn_n'
Cuts at: 190 616 1179 1953 1968 2674 2755 3090 5234
Size: 426 563 774 15 706 81 335 2144
Cuts at: 5234 190
Size: 418
Fragments arranged by size:
2144 774 706 563 426 418 335 81
15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1707 3829 1707
Size: 2122 3340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1502 1642 1663 1764 1873 2125 2197 700
Size: 802 140 21 101 109 252 72 3965
Fragments arranged by size:
3965 802 252 140 109 101 72 21
Bpu10I CC'TnA_GC
Cuts at: 311 2275 311
Size: 1964 3498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 2638 3517 4009 4307 2219
Size: 419 879 492 298 3374
Fragments arranged by size:
3374 879 492 419 298
BsaI GGTCTCn'nnnn_
Cuts at: 483 676 778 2431 5166 483
Size: 193 102 1653 2735 779
Fragments arranged by size:
2735 1653 779 193 102
BsaAI yAC'GTr
Cuts at: 1803 2815 1803
Size: 1012 4450
BsaBI GATnn'nnATC
Cuts at: 6 2976 5013 6
Size: 2970 2037 455
Fragments arranged by size:
2970 2037 455
BsaHI Gr'CG_yC
Cuts at: 2893 3141 3843 5339 2893
Size: 248 702 1496 3016
Fragments arranged by size:
3016 1496 702 248
BsaWI w'CCGG_w
Cuts at: 464 793 1157 2651 3172 3850 4124 4271 4682
Size: 329 364 1494 521 678 274 147 411
Cuts at: 4682 464
Size: 1244
Fragments arranged by size:
1494 1244 678 521 411 364 329 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1636 1666 2314 2344 1636
Size: 30 648 30 4754
Fragments arranged by size:
4754 648 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 581 1369 581
Size: 788 4674
BseYI C'CCAG_C
Cuts at: 194 1230 1719 2887 3645 4222 4718 194
Size: 1036 489 1168 758 577 496 938
Fragments arranged by size:
1168 1036 938 758 577 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 727 1023 1419 2237 673
Size: 54 296 396 818 3898
Fragments arranged by size:
3898 818 396 296 54
BsiEI CG_ry'CG
Cuts at: 104 148 3004 3050 4258 104
Size: 44 2856 46 1208 1308
Fragments arranged by size:
2856 1308 1208 46 44
BsiHKAI G_wGCw'C
Cuts at: 730 971 1518 1802 2276 2476 3254 3444 4236
Size: 241 547 284 474 200 778 190 792
Cuts at: 4236 730
Size: 1956
Fragments arranged by size:
1956 792 778 547 474 284 241 200
190
BsmI GAATG_Cn'
Cuts at: 1027 1027
Size: 5462
Bsp1286I G_dGCh'C
Cuts at: 298 730 971 1471 1499 1509 1518 1802 2102
Size: 432 241 500 28 10 9 284 300
Cuts at: 2102 2276 2476 3087 3180 3254 3444 3506 4236
Size: 174 200 611 93 74 190 62 730
Cuts at: 4236 298
Size: 1524
Fragments arranged by size:
1524 730 611 500 432 300 284 241
200 190 174 93 74 62 28 10
9
BspEI T'CCGG_A
Cuts at: 464 4682 464
Size: 4218 1244
BspHI T'CATG_A
Cuts at: 4638 4638
Size: 5462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1447 1664 2148 2512 2726 3028 3409 1447
Size: 217 484 364 214 302 381 3500
Fragments arranged by size:
3500 484 381 364 302 217 214
BsrBI CCG'CTC
Cuts at: 50 336 3754 3808 5292 5457 50
Size: 286 3418 54 1484 165 55
Fragments arranged by size:
3418 1484 286 165 55 54
BsrDI GCAATG_nn'
Cuts at: 439 1713 3374 439
Size: 1274 1661 2527
Fragments arranged by size:
2527 1661 1274
BsrFI r'CCGG_y
Cuts at: 147 793 1100 1157 1280 1580 2440 3460 3641
Size: 646 307 57 123 300 860 1020 181
Cuts at: 3641 3850 147
Size: 209 1759
Fragments arranged by size:
1759 1020 860 646 307 300 209 181
123 57
BssSI C'ACGA_G
Cuts at: 3733 4091 3733
Size: 358 5104
BstAPI GCAn_nnn'nTGC
Cuts at: 1304 2839 1304
Size: 1535 3927
BstBI TT'CG_AA
Cuts at: 3823 3823
Size: 5462
BstEII G'GTnAC_C
Cuts at: 777 777
Size: 5462
BstXI CCAn_nnnn'nTGG
Cuts at: 591 591
Size: 5462
BstYI r'GATC_y
Cuts at: 1 1073 1271 1826 2381 2487 2954 2977 3312
Size: 1072 198 555 555 106 467 23 335
Cuts at: 3312 3558 4570 4656 4668 5014 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1072 1012 555 555 467 449 346 335
246 198 106 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2395 2395
Size: 5462
BtgI C'CryG_G
Cuts at: 160 215 761 1052 1196 160
Size: 55 546 291 144 4426
Fragments arranged by size:
4426 546 291 144 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1294 3412 3593 3756 1294
Size: 2118 181 163 3000
Fragments arranged by size:
3000 2118 181 163
BtsI GCAGTG_nn'
Cuts at: 124 660 2705 124
Size: 536 2045 2881
Fragments arranged by size:
2881 2045 536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2408 2443 2408
Size: 35 5427
DraI TTT'AAA
Cuts at: 2461 2461
Size: 5462
DraIII CAC_nnn'GTG
Cuts at: 1537 1537
Size: 5462
DrdI GACnn_nn'nnGTC
Cuts at: 3168 4026 3168
Size: 858 4604
EaeI y'GGCC_r
Cuts at: 145 258 698 1582 1592 1865 2360 3047 3221
Size: 113 440 884 10 273 495 687 174
Cuts at: 3221 3612 3639 4984 145
Size: 391 27 1345 623
Fragments arranged by size:
1345 884 687 623 495 440 391 273
174 113 27 10
EagI C'GGCC_G
Cuts at: 145 3047 145
Size: 2902 2560
EarI CTCTTCn'nnn_
Cuts at: 566 1106 1115 1291 1765 3485 3695 566
Size: 540 9 176 474 1720 210 2333
Fragments arranged by size:
2333 1720 540 474 210 176 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1315 3670 3980 4126 5272 1315
Size: 2355 310 146 1146 1505
Fragments arranged by size:
2355 1505 1146 310 146
EcoICRI GAG'CTC
Cuts at: 969 2274 2474 969
Size: 1305 200 3957
Fragments arranged by size:
3957 1305 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 700 871 1195 1502 1642 1663 1764 1789
Size: 286 171 324 307 140 21 101 25
Cuts at: 1789 1873 2125 2197 3287 3719 4466 4734 414
Size: 84 252 72 1090 432 747 268 1142
Fragments arranged by size:
1142 1090 747 432 324 307 286 268
252 171 140 101 84 72 25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1333 2394 1333
Size: 1061 4401
EcoO109I rG'GnC_Cy
Cuts at: 294 295 1131 1343 1467 1468 1495 1638 2601
Size: 1 836 212 124 1 27 143 963
Cuts at: 2601 294
Size: 3155
Fragments arranged by size:
3155 963 836 212 143 124 27 1
1
EcoRI G'AATT_C
Cuts at: 2466 2466
Size: 5462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 444 2037 2335 3481 444
Size: 1593 298 1146 2425
Fragments arranged by size:
2425 1593 1146 298
FspI TGC'GCA
Cuts at: 3243 3243
Size: 5462
HaeII r_GCGC'y
Cuts at: 921 1305 2896 3144 4166 5145 921
Size: 384 1591 248 1022 979 1238
Fragments arranged by size:
1591 1238 1022 979 384 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 480 512 783 815 2059 2091 2100 2132 2314
Size: 32 271 32 1244 32 9 32 182
Cuts at: 2314 2346 3304 3334 3336 3366 480
Size: 32 958 30 2 30 2576
Fragments arranged by size:
2576 1244 958 271 182 32 32 32
32 32 30 30 9 2
HincII GTy'rAC
Cuts at: 1878 2501 1878
Size: 623 4839
KasI G'GCGC_C
Cuts at: 2892 3140 2892
Size: 248 5214
KpnI G_GTAC'C
Cuts at: 342 2482 342
Size: 2140 3322
MluI A'CGCG_T
Cuts at: 4895 4895
Size: 5462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 370 2198 2340 4133 4317 370
Size: 1828 142 1793 184 1515
Fragments arranged by size:
1828 1793 1515 184 142
MscI TGG'CCA
Cuts at: 260 1867 2362 3223 260
Size: 1607 495 861 2499
Fragments arranged by size:
2499 1607 861 495
MslI CAynn'nnrTG
Cuts at: 589 670 1918 3451 3578 589
Size: 81 1248 1533 127 2473
Fragments arranged by size:
2473 1533 1248 127 81
MspA1I CmG'CkG
Cuts at: 670 696 1230 1433 1727 1969 2573 2887 3247
Size: 26 534 203 294 242 604 314 360
Cuts at: 3247 4260 4505 670
Size: 1013 245 1627
Fragments arranged by size:
1627 1013 604 534 360 314 294 245
242 203 26
NaeI GCC'GGC
Cuts at: 149 1282 1582 3643 149
Size: 1133 300 2061 1968
Fragments arranged by size:
2061 1968 1133 300
NarI GG'CG_CC
Cuts at: 2893 3141 2893
Size: 248 5214
NcoI C'CATG_G
Cuts at: 160 1052 1196 160
Size: 892 144 4426
Fragments arranged by size:
4426 892 144
NgoMIV G'CCGG_C
Cuts at: 147 1280 1580 3641 147
Size: 1133 300 2061 1968
Fragments arranged by size:
2061 1968 1133 300
NspI r_CATG'y
Cuts at: 247 953 1716 1758 2515 3015 3922 247
Size: 706 763 42 757 500 907 1787
Fragments arranged by size:
1787 907 763 757 706 500 42
PasI CC'CwG_GG
Cuts at: 1569 1569
Size: 5462
PciI A'CATG_T
Cuts at: 3918 3918
Size: 5462
PflMI CCAn_nnn'nTGG
Cuts at: 113 1053 1230 113
Size: 940 177 4345
Fragments arranged by size:
4345 940 177
PfoI T'CCnGG_A
Cuts at: 1076 1415 1741 2198 1076
Size: 339 326 457 4340
Fragments arranged by size:
4340 457 339 326
PmeI GTTT'AAAC
Cuts at: 2461 2461
Size: 5462
PmlI CAC'GTG
Cuts at: 1803 1803
Size: 5462
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1894 1926 4601 4633 1894
Size: 32 2675 32 2723
Fragments arranged by size:
2723 2675 32 32
PpuMI rG'GwC_Cy
Cuts at: 1343 1638 1343
Size: 295 5167
PspOMI G'GGCC_C
Cuts at: 294 1467 1495 294
Size: 1173 28 4261
Fragments arranged by size:
4261 1173 28
PstI C_TGCA'G
Cuts at: 811 933 1073 1664 2509 3194 811
Size: 122 140 591 845 685 3079
Fragments arranged by size:
3079 845 685 591 140 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5462
PvuII CAG'CTG
Cuts at: 670 696 1230 1433 1727 1969 2887 3247 670
Size: 26 534 203 294 242 918 360 2885
Fragments arranged by size:
2885 918 534 360 294 242 203 26
RsrII CG'GwC_CG
Cuts at: 3657 3657
Size: 5462
SacI G_AGCT'C
Cuts at: 971 2276 2476 971
Size: 1305 200 3957
Fragments arranged by size:
3957 1305 200
SalI G'TCGA_C
Cuts at: 2499 2499
Size: 5462
SapI GCTCTTCn'nnn_
Cuts at: 1291 1765 3485 3695 1291
Size: 474 1720 210 3058
Fragments arranged by size:
3058 1720 474 210
SbfI CC_TGCA'GG
Cuts at: 2509 2509
Size: 5462
ScaI AGT'ACT
Cuts at: 4979 4979
Size: 5462
SexAI A'CCwGG_T
Cuts at: 358 358
Size: 5462
SfcI C'TryA_G
Cuts at: 33 807 929 1069 1660 2169 2451 2505 3190
Size: 774 122 140 591 509 282 54 685
Cuts at: 3190 4183 4374 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 774 685 591 509 282 191
140 122 54
SfoI GGC'GCC
Cuts at: 2894 3142 2894
Size: 248 5214
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
SmaI CCC'GGG
Cuts at: 1553 2484 1553
Size: 931 4531
SmlI C'TyrA_G
Cuts at: 2234 2353 2617 3532 4024 4286 2234
Size: 119 264 915 492 262 3410
Fragments arranged by size:
3410 915 492 264 262 119
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5462
SphI G_CATG'C
Cuts at: 247 1758 2515 3015 247
Size: 1511 757 500 2694
Fragments arranged by size:
2694 1511 757 500
StyI C'CwwG_G
Cuts at: 160 480 561 1052 1196 1334 1484 1997 2596
Size: 320 81 491 144 138 150 513 599
Cuts at: 2596 2950 160
Size: 354 2672
Fragments arranged by size:
2672 599 513 491 354 320 150 144
138 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 186 3045 3178 3637 3846 186
Size: 2859 133 459 209 1802
Fragments arranged by size:
2859 1802 459 209 133
TatI w'GTAC_w
Cuts at: 870 1781 4977 870
Size: 911 3196 1355
Fragments arranged by size:
3196 1355 911
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4979 5011 4979
Size: 32 5430
Tth111I GACn'n_nGTC
Cuts at: 832 3259 832
Size: 2427 3035
XbaI T'CTAG_A
Cuts at: 2493 2493
Size: 5462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2370 2957 2370
Size: 587 4875
XmaI C'CCGG_G
Cuts at: 1551 2482 1551
Size: 931 4531
XmnI GAAnn'nnTTC
Cuts at: 424 1610 2017 424
Size: 1186 407 3869
Fragments arranged by size:
3869 1186 407
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AhdI AloI AscI AvrII BaeI BbvCI BfrBI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI