Restriction map for
pF1KA1745
(Circular) (Six-base) MAPSORT of: pF1KA1745.seq Check: 6881 from: 1 to: 5624
pF1KA1745 5624 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2662 2662
Size: 5624
Acc65I G'GTAC_C
Cuts at: 2465 2640 2465
Size: 175 5449
AclI AA'CG_TT
Cuts at: 5464 5464
Size: 5624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 552 882 1200 2371 3449 3881 4628 4896
Size: 262 330 318 1171 1078 432 747 268
Cuts at: 4896 290
Size: 1018
Fragments arranged by size:
1171 1078 1018 747 432 330 318 268
262
AfeI AGC'GCT
Cuts at: 1003 1003
Size: 5624
AflIII A'CryG_T
Cuts at: 2976 4080 5057 2976
Size: 1104 977 3543
Fragments arranged by size:
3543 1104 977
AgeI A'CCGG_T
Cuts at: 4012 4012
Size: 5624
AleI CACnn'nnGTG
Cuts at: 940 1254 940
Size: 314 5310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1438 1438
Size: 5624
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 907 939 907
Size: 32 5592
AlwNI CAG_nnn'CTG
Cuts at: 429 1032 1608 1700 2103 2117 2475 2929 4496
Size: 603 576 92 403 14 358 454 1567
Cuts at: 4496 429
Size: 1557
Fragments arranged by size:
1567 1557 603 576 454 403 358 92
14
ApaI G_GGCC'C
Cuts at: 1549 1989 2438 1549
Size: 440 449 4735
Fragments arranged by size:
4735 449 440
ApaLI G'TGCA_C
Cuts at: 1555 2204 2271 2374 4394 1555
Size: 649 67 103 2020 2785
Fragments arranged by size:
2785 2020 649 103 67
ApoI r'AATT_y
Cuts at: 675 916 2628 4977 5233 675
Size: 241 1712 2349 256 1066
Fragments arranged by size:
2349 1712 1066 256 241
AseI AT'TA_AT
Cuts at: 20 20
Size: 5624
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
AvaI C'yCGr_G
Cuts at: 365 1319 1550 1653 1721 2490 2498 2579 2644
Size: 954 231 103 68 769 8 81 65
Cuts at: 2644 365
Size: 3345
Fragments arranged by size:
3345 954 769 231 103 81 68 65
8
AvrII C'CTAG_G
Cuts at: 2430 2430
Size: 5624
BamHI G'GATC_C
Cuts at: 1802 2649 1802
Size: 847 4777
BanI G'GyrC_C
Cuts at: 1278 1668 2047 2324 2465 2640 3054 3302 3337
Size: 390 379 277 141 175 414 248 35
Cuts at: 3337 1278
Size: 3565
Fragments arranged by size:
3565 414 390 379 277 248 175 141
35
BanII G_rGCy'C
Cuts at: 268 713 799 1096 1549 1989 2438 2638 3668
Size: 445 86 297 453 440 449 200 1030
Cuts at: 3668 268
Size: 2224
Fragments arranged by size:
2224 1030 453 449 445 440 297 200
86
BbeI G_GCGC'C
Cuts at: 3058 3306 3058
Size: 248 5376
BbsI GAAGACnn'nnnn_
Cuts at: 1074 1128 1179 1206 1841 2337 2496 1074
Size: 54 51 27 635 496 159 4202
Fragments arranged by size:
4202 635 496 159 54 51 27
BbvCI CC'TCA_GC
Cuts at: 397 834 1781 397
Size: 437 947 4240
Fragments arranged by size:
4240 947 437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2433 2467 90
Size: 34 2309 34 3247
Fragments arranged by size:
3247 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3522 4283 5624 2568
Size: 954 761 1341 2568
Fragments arranged by size:
2568 1341 954 761
BclI T'GATC_A
Cuts at: 3144 3144
Size: 5624
BglI GCCn_nnn'nGGC
Cuts at: 171 171
Size: 5624
BglII A'GATC_T
Cuts at: 884 1577 3139 5176 884
Size: 693 1562 2037 1332
Fragments arranged by size:
2037 1562 1332 693
BlpI GC'TnA_GC
Cuts at: 238 331 529 1090 2736 238
Size: 93 198 561 1646 3126
Fragments arranged by size:
3126 1646 561 198 93
Bme1580I G_kGCm'C
Cuts at: 1283 1320 1510 1549 1559 1673 1989 2050 2208
Size: 37 190 39 10 114 316 61 158
Cuts at: 2208 2275 2378 2438 2572 3249 3342 4398 1283
Size: 67 103 60 134 677 93 1056 2509
Fragments arranged by size:
2509 1056 677 316 190 158 134 114
103 93 67 61 60 39 37 10
BmgBI CAC'GTC
Cuts at: 1764 1764
Size: 5624
BmrI ACTGGGnnnn_n'
Cuts at: 1140 2036 2296 2557 2836 2917 3252 5396 1140
Size: 896 260 261 279 81 335 2144 1368
Fragments arranged by size:
2144 1368 896 335 279 261 260 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 391 3991 391
Size: 3600 2024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1734 1316
Size: 418 5206
Bpu10I CC'TnA_GC
Cuts at: 397 834 1781 397
Size: 437 947 4240
Fragments arranged by size:
4240 947 437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1360 2800 3679 4171 4469 1003
Size: 357 1440 879 492 298 2158
Fragments arranged by size:
2158 1440 879 492 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 2404 5328 2404
Size: 2924 2700
BsaAI yAC'GTr
Cuts at: 357 2016 2977 357
Size: 1659 961 3004
Fragments arranged by size:
3004 1659 961
BsaBI GATnn'nnATC
Cuts at: 6 3138 5175 6
Size: 3132 2037 455
Fragments arranged by size:
3132 2037 455
BsaHI Gr'CG_yC
Cuts at: 3055 3303 4005 5501 3055
Size: 248 702 1496 3178
Fragments arranged by size:
3178 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2327 2813 3334 4012 4286 4433 4844 2327
Size: 486 521 678 274 147 411 3107
Fragments arranged by size:
3107 678 521 486 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 974 1004 2056 2086 974
Size: 30 1052 30 4512
Fragments arranged by size:
4512 1052 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 1582 1705 1936 2101 147
Size: 1435 123 231 165 3670
Fragments arranged by size:
3670 1435 231 165 123
BseYI C'CCAG_C
Cuts at: 819 1025 1096 2058 2228 3049 3807 4384 4880
Size: 206 71 962 170 821 758 577 496
Cuts at: 4880 819
Size: 1563
Fragments arranged by size:
1563 962 821 758 577 496 206 170
71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 486 1225 1462 1621 2255 461
Size: 25 739 237 159 634 3830
Fragments arranged by size:
3830 739 634 237 159 25
BsiEI CG_ry'CG
Cuts at: 104 1521 3166 3212 4420 104
Size: 1417 1645 46 1208 1308
Fragments arranged by size:
1645 1417 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 366 713 799 1039 1559 2208 2275 2378 2638
Size: 347 86 240 520 649 67 103 260
Cuts at: 2638 3416 3606 4398 366
Size: 778 190 792 1592
Fragments arranged by size:
1592 792 778 649 520 347 260 240
190 103 86 67
Bsp1286I G_dGCh'C
Cuts at: 268 366 713 799 1039 1096 1283 1320 1510
Size: 98 347 86 240 57 187 37 190
Cuts at: 1510 1549 1559 1673 1989 2050 2208 2275 2378
Size: 39 10 114 316 61 158 67 103
Cuts at: 2378 2438 2572 2638 3249 3342 3416 3606 3668
Size: 60 134 66 611 93 74 190 62
Cuts at: 3668 4398 268
Size: 730 1494
Fragments arranged by size:
1494 730 611 347 316 240 190 190
187 158 134 114 103 98 93 86
74 67 66 62 61 60 57 39
37 10
BspEI T'CCGG_A
Cuts at: 4844 4844
Size: 5624
BspHI T'CATG_A
Cuts at: 4800 4800
Size: 5624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1392 2042 2395 2674 2888 3190 3571 1392
Size: 650 353 279 214 302 381 3445
Fragments arranged by size:
3445 650 381 353 302 279 214
BsrBI CCG'CTC
Cuts at: 50 276 528 987 1745 1944 2451 3916 3970
Size: 226 252 459 758 199 507 1465 54
Cuts at: 3970 5454 5619 50
Size: 1484 165 55
Fragments arranged by size:
1484 1465 758 507 459 252 226 199
165 55 54
BsrDI GCAATG_nn'
Cuts at: 950 3536 950
Size: 2586 3038
BsrFI r'CCGG_y
Cuts at: 1520 2321 3622 3803 4012 1520
Size: 801 1301 181 209 3132
Fragments arranged by size:
3132 1301 801 209 181
BsrGI T'GTAC_A
Cuts at: 1458 1691 1458
Size: 233 5391
BssHII G'CGCG_C
Cuts at: 158 158
Size: 5624
BssSI C'ACGA_G
Cuts at: 3895 4253 3895
Size: 358 5266
BstAPI GCAn_nnn'nTGC
Cuts at: 1608 3001 1608
Size: 1393 4231
BstBI TT'CG_AA
Cuts at: 293 3985 293
Size: 3692 1932
BstXI CCAn_nnnn'nTGG
Cuts at: 693 693
Size: 5624
BstYI r'GATC_y
Cuts at: 1 515 884 1577 1802 2600 2649 3116 3139
Size: 514 369 693 225 798 49 467 23
Cuts at: 3139 3474 3720 4732 4818 4830 5176 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 798 693 514 467 449 369 346
335 246 225 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 130 130
Size: 5624
BtgI C'CryG_G
Cuts at: 151 1673 1798 151
Size: 1522 125 3977
Fragments arranged by size:
3977 1522 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 136 887 986 2177 3574 3755 3918 136
Size: 751 99 1191 1397 181 163 1842
Fragments arranged by size:
1842 1397 1191 751 181 163 99
BtsI GCAGTG_nn'
Cuts at: 469 686 1261 1541 2175 2867 469
Size: 217 575 280 634 692 3226
Fragments arranged by size:
3226 692 634 575 280 217
DraI TTT'AAA
Cuts at: 2623 2623
Size: 5624
DraIII CAC_nnn'GTG
Cuts at: 1278 1278
Size: 5624
DrdI GACnn_nn'nnGTC
Cuts at: 1402 3330 4188 1402
Size: 1928 858 2838
Fragments arranged by size:
2838 1928 858
EaeI y'GGCC_r
Cuts at: 172 277 752 909 1518 1676 1787 2288 3209
Size: 105 475 157 609 158 111 501 921
Cuts at: 3209 3383 3774 3801 5146 172
Size: 174 391 27 1345 650
Fragments arranged by size:
1345 921 650 609 501 475 391 174
158 157 111 105 27
EagI C'GGCC_G
Cuts at: 1518 3209 1518
Size: 1691 3933
EarI CTCTTCn'nnn_
Cuts at: 266 1504 2515 3647 3857 266
Size: 1238 1011 1132 210 2033
Fragments arranged by size:
2033 1238 1132 1011 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 3832 4142 4288 5434 2575
Size: 1257 310 146 1146 2765
Fragments arranged by size:
2765 1257 1146 310 146
EcoICRI GAG'CTC
Cuts at: 711 797 2636 711
Size: 86 1839 3699
Fragments arranged by size:
3699 1839 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 552 882 1200 1316 1734 2371 3449 3881
Size: 262 330 318 116 418 637 1078 432
Cuts at: 3881 4628 4896 290
Size: 747 268 1018
Fragments arranged by size:
1078 1018 747 637 432 418 330 318
268 262 116
EcoNI CCTnn'n_nnAGG
Cuts at: 1228 2353 1228
Size: 1125 4499
EcoO109I rG'GnC_Cy
Cuts at: 347 372 483 1546 1725 1833 1985 1986 2031
Size: 25 111 1063 179 108 152 1 45
Cuts at: 2031 2243 2434 2435 2495 2763 347
Size: 212 191 1 60 268 3208
Fragments arranged by size:
3208 1063 268 212 191 179 152 111
108 60 45 25 1 1
EcoRI G'AATT_C
Cuts at: 2628 2628
Size: 5624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 906 1401 3643 906
Size: 495 2242 2887
Fragments arranged by size:
2887 2242 495
FspI TGC'GCA
Cuts at: 115 3405 115
Size: 3290 2334
HaeII r_GCGC'y
Cuts at: 238 1005 1829 3058 3306 4328 5307 238
Size: 767 824 1229 248 1022 979 555
Fragments arranged by size:
1229 1022 979 824 767 555 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1289 1321 3466 3496 3498 3528 1289
Size: 32 2145 30 2 30 3385
Fragments arranged by size:
3385 2145 32 30 30 2
HincII GTy'rAC
Cuts at: 2663 2663
Size: 5624
KasI G'GCGC_C
Cuts at: 3054 3302 3054
Size: 248 5376
KpnI G_GTAC'C
Cuts at: 2469 2644 2469
Size: 175 5449
MluI A'CGCG_T
Cuts at: 5057 5057
Size: 5624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 250 335 1976 4295 4479 250
Size: 85 1641 2319 184 1395
Fragments arranged by size:
2319 1641 1395 184 85
MscI TGG'CCA
Cuts at: 911 1678 1789 3385 911
Size: 767 111 1596 3150
Fragments arranged by size:
3150 1596 767 111
MslI CAynn'nnrTG
Cuts at: 121 592 940 1254 3613 3740 121
Size: 471 348 314 2359 127 2005
Fragments arranged by size:
2359 2005 471 348 314 127
MspA1I CmG'CkG
Cuts at: 180 533 1029 1220 1754 1785 2058 2103 2212
Size: 353 496 191 534 31 273 45 109
Cuts at: 2212 2228 2735 3049 3409 4422 4667 180
Size: 16 507 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 534 507 496 360 353 314
273 245 191 109 45 31 16
NaeI GCC'GGC
Cuts at: 1522 3805 1522
Size: 2283 3341
NarI GG'CG_CC
Cuts at: 3055 3303 3055
Size: 248 5376
NcoI C'CATG_G
Cuts at: 151 1673 151
Size: 1522 4102
NgoMIV G'CCGG_C
Cuts at: 1520 3803 1520
Size: 2283 3341
NspI r_CATG'y
Cuts at: 1419 2677 3177 4084 1419
Size: 1258 500 907 2959
Fragments arranged by size:
2959 1258 907 500
PciI A'CATG_T
Cuts at: 4080 4080
Size: 5624
PflMI CCAn_nnn'nTGG
Cuts at: 1452 1799 2058 2103 2215 1452
Size: 347 259 45 112 4861
Fragments arranged by size:
4861 347 259 112 45
PfoI T'CCnGG_A
Cuts at: 638 1613 1805 638
Size: 975 192 4457
Fragments arranged by size:
4457 975 192
PmeI GTTT'AAAC
Cuts at: 2623 2623
Size: 5624
PmlI CAC'GTG
Cuts at: 2016 2016
Size: 5624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 4763
Size: 32 5592
PpuMI rG'GwC_Cy
Cuts at: 347 483 1725 1833 2031 2243 2495 347
Size: 136 1242 108 198 212 252 3476
Fragments arranged by size:
3476 1242 252 212 198 136 108
PspOMI G'GGCC_C
Cuts at: 1545 1985 2434 1545
Size: 440 449 4735
Fragments arranged by size:
4735 449 440
PstI C_TGCA'G
Cuts at: 215 1076 1349 1430 1577 1687 2671 3356 215
Size: 861 273 81 147 110 984 685 2483
Fragments arranged by size:
2483 984 861 685 273 147 110 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5624
PvuII CAG'CTG
Cuts at: 1029 1754 1785 2058 2103 2212 2228 3049 3409
Size: 725 31 273 45 109 16 821 360
Cuts at: 3409 1029
Size: 3244
Fragments arranged by size:
3244 821 725 360 273 109 45 31
16
RsrII CG'GwC_CG
Cuts at: 3819 3819
Size: 5624
SacI G_AGCT'C
Cuts at: 713 799 2638 713
Size: 86 1839 3699
Fragments arranged by size:
3699 1839 86
SalI G'TCGA_C
Cuts at: 2661 2661
Size: 5624
SanDI GG'GwC_CC
Cuts at: 1725 2495 1725
Size: 770 4854
SapI GCTCTTCn'nnn_
Cuts at: 266 3647 3857 266
Size: 3381 210 2033
Fragments arranged by size:
3381 2033 210
SbfI CC_TGCA'GG
Cuts at: 2671 2671
Size: 5624
ScaI AGT'ACT
Cuts at: 5141 5141
Size: 5624
SfcI C'TryA_G
Cuts at: 33 211 994 1072 1163 1345 1426 1573 1683
Size: 178 783 78 91 182 81 147 110
Cuts at: 1683 2667 3352 4345 4536 33
Size: 984 685 993 191 1121
Fragments arranged by size:
1121 993 984 783 685 191 182 178
147 110 91 81 78
SfoI GGC'GCC
Cuts at: 3056 3304 3056
Size: 248 5376
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
SmaI CCC'GGG
Cuts at: 1321 1552 1723 2492 2581 2646 1321
Size: 231 171 769 89 65 4299
Fragments arranged by size:
4299 769 231 171 89 65
SmlI C'TyrA_G
Cuts at: 365 1018 1375 2779 3694 4186 4448 365
Size: 653 357 1404 915 492 262 1541
Fragments arranged by size:
1541 1404 915 653 492 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5624
SphI G_CATG'C
Cuts at: 1419 2677 3177 1419
Size: 1258 500 3866
Fragments arranged by size:
3866 1258 500
StyI C'CwwG_G
Cuts at: 151 738 1673 1829 2430 2589 2758 3112 151
Size: 587 935 156 601 159 169 354 2663
Fragments arranged by size:
2663 935 601 587 354 169 159 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 805 1636 2065 3207 3340 3799 4008 349
Size: 456 831 429 1142 133 459 209 1965
Fragments arranged by size:
1965 1142 831 459 456 429 209 133
TatI w'GTAC_w
Cuts at: 1458 1691 5139 1458
Size: 233 3448 1943
Fragments arranged by size:
3448 1943 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 974 1006 1583 1615 2056 2088 2519 2551 5141
Size: 32 577 32 441 32 431 32 2590
Cuts at: 5141 5173 974
Size: 32 1425
Fragments arranged by size:
2590 1425 577 441 431 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3421 3421
Size: 5624
XbaI T'CTAG_A
Cuts at: 2655 2655
Size: 5624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 3119 693
Size: 2426 3198
XhoI C'TCGA_G
Cuts at: 365 365
Size: 5624
XmaI C'CCGG_G
Cuts at: 1319 1550 1721 2490 2579 2644 1319
Size: 231 171 769 89 65 4299
Fragments arranged by size:
4299 769 231 171 89 65
XmnI GAAnn'nnTTC
Cuts at: 2257 2344 2257
Size: 87 5537
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI BaeI BfrBI BmtI
BsiWI BsmI BsmBI BstEII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI