Restriction map for
pF1KA1732
(Circular) (Six-base) MAPSORT of: pF1KA1732.seq Check: 6225 from: 1 to: 11039
pF1KA1732 11039 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 6504 702
Size: 5802 5237
AccI GT'mk_AC
Cuts at: 437 656 3202 3792 4041 8077 437
Size: 219 2546 590 249 4036 3399
Fragments arranged by size:
4036 3399 2546 590 249 219
Acc65I G'GTAC_C
Cuts at: 8055 8055
Size: 11039
AclI AA'CG_TT
Cuts at: 5329 10879 5329
Size: 5550 5489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 321 415 1070 1655 2534 2549 2740 4171
Size: 124 94 655 585 879 15 191 1431
Cuts at: 4171 4354 4452 4954 5297 5662 5713 6465 7309
Size: 183 98 502 343 365 51 752 844
Cuts at: 7309 7868 7924 8864 9296 9649 10311 197
Size: 559 56 940 432 353 662 925
Fragments arranged by size:
1431 940 925 879 844 752 662 655
585 559 502 432 365 353 343 191
183 124 98 94 56 51 15
AflIII A'CryG_T
Cuts at: 903 3072 3840 3994 7023 7243 8391 10191 10472
Size: 2169 768 154 3029 220 1148 1800 281
Cuts at: 10472 903
Size: 1470
Fragments arranged by size:
3029 2169 1800 1470 1148 768 281 220
154
AhdI GACnn_n'nnGTC
Cuts at: 4200 4200
Size: 11039
AleI CACnn'nnGTG
Cuts at: 5231 5231
Size: 11039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2181 2181
Size: 11039
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1706 1738 1706
Size: 32 11007
AlwNI CAG_nnn'CTG
Cuts at: 1075 2149 2695 5944 6208 6771 7036 7797 8344
Size: 1074 546 3249 264 563 265 761 547
Cuts at: 8344 9782 1075
Size: 1438 2332
Fragments arranged by size:
3249 2332 1438 1074 761 563 547 546
265 264
ApaI G_GGCC'C
Cuts at: 4448 4448
Size: 11039
ApaLI G'TGCA_C
Cuts at: 9877 9877
Size: 11039
ApoI r'AATT_y
Cuts at: 530 980 1126 1810 1818 2023 2625 2644 3006
Size: 450 146 684 8 205 602 19 362
Cuts at: 3006 3123 3330 3454 3533 3660 4171 6413 8043
Size: 117 207 124 79 127 511 2242 1630
Cuts at: 8043 10392 10648 530
Size: 2349 256 921
Fragments arranged by size:
2349 2242 1630 921 684 602 511 450
362 256 207 205 146 127 124 117
79 19 8
AseI AT'TA_AT
Cuts at: 20 1916 6263 20
Size: 1896 4347 4796
Fragments arranged by size:
4796 4347 1896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11039
AvaI C'yCGr_G
Cuts at: 4880 7535 8059 4880
Size: 2655 524 7860
Fragments arranged by size:
7860 2655 524
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2438 2471 3946 3979 2438
Size: 33 1475 33 9498
Fragments arranged by size:
9498 1475 33 33
BamHI G'GATC_C
Cuts at: 4026 4144 6873 7593 8064 4026
Size: 118 2729 720 471 7001
Fragments arranged by size:
7001 2729 720 471 118
BanI G'GyrC_C
Cuts at: 280 5069 7017 8055 8469 8717 8752 280
Size: 4789 1948 1038 414 248 35 2567
Fragments arranged by size:
4789 2567 1948 1038 414 248 35
BanII G_rGCy'C
Cuts at: 1171 1420 3872 4448 4638 6227 8053 9083 1171
Size: 249 2452 576 190 1589 1826 1030 3127
Fragments arranged by size:
3127 2452 1826 1589 1030 576 249 190
BbeI G_GCGC'C
Cuts at: 5073 8473 8721 5073
Size: 3400 248 7391
Fragments arranged by size:
7391 3400 248
BbsI GAAGACnn'nnnn_
Cuts at: 1513 2755 3039 3143 4669 6511 7315 7534 7856
Size: 1242 284 104 1526 1842 804 219 322
Cuts at: 7856 1513
Size: 4696
Fragments arranged by size:
4696 1842 1526 1242 804 322 284 219
104
BbvCI CC'TCA_GC
Cuts at: 5041 7424 5041
Size: 2383 8656
BciVI GTATCCnnnnn_n'
Cuts at: 8937 9993 11039 8937
Size: 1056 1046 8937
Fragments arranged by size:
8937 1056 1046
BclI T'GATC_A
Cuts at: 2088 6069 6951 7571 8559 2088
Size: 3981 882 620 988 4568
Fragments arranged by size:
4568 3981 988 882 620
BfrBI ATG'CAT
Cuts at: 2249 3160 2249
Size: 911 10128
BglII A'GATC_T
Cuts at: 814 1318 4989 8554 10591 814
Size: 504 3671 3565 2037 1262
Fragments arranged by size:
3671 3565 2037 1262 504
BlpI GC'TnA_GC
Cuts at: 1170 5548 5932 6959 8151 1170
Size: 4378 384 1027 1192 4058
Fragments arranged by size:
4378 4058 1192 1027 384
Bme1580I G_kGCm'C
Cuts at: 4424 4448 8664 8757 9881 4424
Size: 24 4216 93 1124 5582
Fragments arranged by size:
5582 4216 1124 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 1726 3805 4032 4064 6922 7050 7126 7773 8251
Size: 2079 227 32 2858 128 76 647 478
Cuts at: 8251 8332 8667 10811 1726
Size: 81 335 2144 1954
Fragments arranged by size:
2858 2144 2079 1954 647 478 335 227
128 81 76 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1337 1359 4873 4974 5544 9406 1337
Size: 22 3514 101 570 3862 2970
Fragments arranged by size:
3862 3514 2970 570 101 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1934 2807 3289 4994 7080 7713 7951 401
Size: 1533 873 482 1705 2086 633 238 3489
Fragments arranged by size:
3489 2086 1705 1533 873 633 482 238
Bpu10I CC'TnA_GC
Cuts at: 5041 7424 5041
Size: 2383 8656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1500 2978 3881 4061 4622 4818 7199 7654 8215
Size: 1478 903 180 561 196 2381 455 561
Cuts at: 8215 9094 9808 10106 1500
Size: 879 714 298 2433
Fragments arranged by size:
2433 2381 1478 903 879 714 561 561
455 298 196 180
BsaI GGTCTCn'nnnn_
Cuts at: 1544 1594 1952 5147 5530 5828 6524 10743 1544
Size: 50 358 3195 383 298 696 4219 1840
Fragments arranged by size:
4219 3195 1840 696 383 358 298 50
BsaAI yAC'GTr
Cuts at: 906 3917 3997 8392 906
Size: 3011 80 4395 3553
Fragments arranged by size:
4395 3553 3011 80
BsaBI GATnn'nnATC
Cuts at: 6 1290 2717 6068 8553 10590 6
Size: 1284 1427 3351 2485 2037 455
Fragments arranged by size:
3351 2485 2037 1427 1284 455
BsaHI Gr'CG_yC
Cuts at: 5070 8470 8718 9420 10916 5070
Size: 3400 248 702 1496 5193
Fragments arranged by size:
5193 3400 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3528 7808 8228 8749 9427 9838 9985 10259 3528
Size: 4280 420 521 678 411 147 274 4308
Fragments arranged by size:
4308 4280 678 521 420 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2724 2754 3059 3089 5940 5970 6110 6140 6286
Size: 30 305 30 2851 30 140 30 146
Cuts at: 6286 6316 7078 7108 7361 7391 7891 7921 2724
Size: 30 762 30 253 30 500 30 5842
Fragments arranged by size:
5842 2851 762 500 305 253 146 140
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3074 4135 5426 6307 7478 3074
Size: 1061 1291 881 1171 6635
Fragments arranged by size:
6635 1291 1171 1061 881
BseYI C'CCAG_C
Cuts at: 1942 4790 5057 6877 6916 7313 8464 9222 9887
Size: 2848 267 1820 39 397 1151 758 665
Cuts at: 9887 10295 1942
Size: 408 2686
Fragments arranged by size:
2848 2686 1820 1151 758 665 408 397
267 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 362 4999 7190 232
Size: 130 4637 2191 4081
Fragments arranged by size:
4637 4081 2191 130
BsiEI CG_ry'CG
Cuts at: 104 5709 8581 8627 9857 104
Size: 5605 2872 46 1230 1286
Fragments arranged by size:
5605 2872 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 167 1171 1420 1473 3872 4638 5549 5895 5938
Size: 1004 249 53 2399 766 911 346 43
Cuts at: 5938 6905 8053 8831 9021 9881 167
Size: 967 1148 778 190 860 1325
Fragments arranged by size:
2399 1325 1148 1004 967 911 860 778
766 346 249 190 53 43
BsmI GAATG_Cn'
Cuts at: 4601 6151 4601
Size: 1550 9489
BsmBI CGTCTCn'nnnn_
Cuts at: 3307 4939 5105 3307
Size: 1632 166 9241
Fragments arranged by size:
9241 1632 166
Bsp1286I G_dGCh'C
Cuts at: 167 1171 1420 1473 3872 4424 4448 4638 5549
Size: 1004 249 53 2399 552 24 190 911
Cuts at: 5549 5895 5938 6227 6905 8053 8664 8757 8831
Size: 346 43 289 678 1148 611 93 74
Cuts at: 8831 9021 9083 9881 167
Size: 190 62 798 1325
Fragments arranged by size:
2399 1325 1148 1004 911 798 678 611
552 346 289 249 190 190 93 74
62 53 43 24
BspEI T'CCGG_A
Cuts at: 3528 3528
Size: 11039
BspHI T'CATG_A
Cuts at: 3210 4682 5219 9471 3210
Size: 1472 537 4252 4778
Fragments arranged by size:
4778 4252 1472 537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 2320 6504 8089 8303 8605 8986 702
Size: 1618 4184 1585 214 302 381 2755
Fragments arranged by size:
4184 2755 1618 1585 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6609 9331 9385 10869 11034 50
Size: 6559 2722 54 1484 165 55
Fragments arranged by size:
6559 2722 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 393 4020 7944 8951 393
Size: 3627 3924 1007 2481
Fragments arranged by size:
3924 3627 2481 1007
BsrFI r'CCGG_y
Cuts at: 7119 9037 9218 7119
Size: 1918 181 8940
Fragments arranged by size:
8940 1918 181
BsrGI T'GTAC_A
Cuts at: 1831 4609 7185 7299 1831
Size: 2778 2576 114 5571
Fragments arranged by size:
5571 2778 2576 114
BssSI C'ACGA_G
Cuts at: 1472 9310 10018 1472
Size: 7838 708 2493
Fragments arranged by size:
7838 2493 708
BstAPI GCAn_nnn'nTGC
Cuts at: 5010 6762 8416 5010
Size: 1752 1654 7633
Fragments arranged by size:
7633 1752 1654
BstBI TT'CG_AA
Cuts at: 4822 9400 4822
Size: 4578 6461
BstEII G'GTnAC_C
Cuts at: 3652 5401 6838 3652
Size: 1749 1437 7853
Fragments arranged by size:
7853 1749 1437
BstXI CCAn_nnnn'nTGG
Cuts at: 4484 5304 5353 6378 7075 4484
Size: 820 49 1025 697 8448
Fragments arranged by size:
8448 1025 820 697 49
BstYI r'GATC_y
Cuts at: 1 814 1191 1318 2131 4026 4144 4989 5681
Size: 813 377 127 813 1895 118 845 692
Cuts at: 5681 6063 6873 7470 7539 7593 8064 8531 8554
Size: 382 810 597 69 54 471 467 23
Cuts at: 8554 8889 9135 9441 9453 9539 10591 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1895 1052 845 813 813 810 692 597
471 467 449 382 377 335 306 246
127 118 86 69 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 1555 7922 1555
Size: 6367 4672
BtgI C'CryG_G
Cuts at: 3131 3344 3131
Size: 213 10826
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4497 5870 8989 9170 9333 4497
Size: 1373 3119 181 163 6203
Fragments arranged by size:
6203 3119 1373 181 163
BtsI GCAGTG_nn'
Cuts at: 374 571 638 2843 4606 4987 4999 6787 7400
Size: 197 67 2205 1763 381 12 1788 613
Cuts at: 7400 8282 374
Size: 882 3131
Fragments arranged by size:
3131 2205 1788 1763 882 613 381 197
67 12
ClaI AT'CG_AT
Cuts at: 1965 1965
Size: 11039
DraI TTT'AAA
Cuts at: 1124 1161 1181 1815 1988 2870 4875 7857 8038
Size: 37 20 634 173 882 2005 2982 181
Cuts at: 8038 1124
Size: 4125
Fragments arranged by size:
4125 2982 2005 882 634 181 173 37
20
DrdI GACnn_nn'nnGTC
Cuts at: 7244 8745 10089 7244
Size: 1501 1344 8194
Fragments arranged by size:
8194 1501 1344
EaeI y'GGCC_r
Cuts at: 1450 5131 5706 6365 8624 8798 9189 9216 10561
Size: 3681 575 659 2259 174 391 27 1345
Cuts at: 10561 1450
Size: 1928
Fragments arranged by size:
3681 2259 1928 1345 659 575 391 174
27
EagI C'GGCC_G
Cuts at: 5706 8624 5706
Size: 2918 8121
EarI CTCTTCn'nnn_
Cuts at: 434 1490 1545 1620 2759 3021 3234 3701 3860
Size: 1056 55 75 1139 262 213 467 159
Cuts at: 3860 4112 5729 6092 6284 6600 9062 9272 434
Size: 252 1617 363 192 316 2462 210 2201
Fragments arranged by size:
2462 2201 1617 1139 1056 467 363 316
262 252 213 210 192 159 75 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 665 1326 9247 9989 10135 10849 665
Size: 661 7921 742 146 714 855
Fragments arranged by size:
7921 855 742 714 661 146
EcoICRI GAG'CTC
Cuts at: 1169 1418 3870 4636 8051 1169
Size: 249 2452 766 3415 4157
Fragments arranged by size:
4157 3415 2452 766 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 321 401 415 1070 1655 1934 2534 2549
Size: 124 80 14 655 585 279 600 15
Cuts at: 2549 2740 2807 3289 4171 4354 4452 4954 4994
Size: 191 67 482 882 183 98 502 40
Cuts at: 4994 5297 5662 5713 6465 7080 7309 7713 7868
Size: 303 365 51 752 615 229 404 155
Cuts at: 7868 7924 7951 8864 9296 9649 10311 197
Size: 56 27 913 432 353 662 925
Fragments arranged by size:
925 913 882 752 662 655 615 600
585 502 482 432 404 365 353 303
279 229 191 183 155 124 98 80
67 56 51 40 27 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1365 2921 6400 1365
Size: 1556 3479 6004
Fragments arranged by size:
6004 3479 1556
EcoO109I rG'GnC_Cy
Cuts at: 4312 4445 4473 7926 8178 4312
Size: 133 28 3453 252 7173
Fragments arranged by size:
7173 3453 252 133 28
EcoRI G'AATT_C
Cuts at: 3006 3330 6413 8043 3006
Size: 324 3083 1630 6002
Fragments arranged by size:
6002 3083 1630 324
EcoRV GAT'ATC
Cuts at: 4299 4299
Size: 11039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4684 6082 9058 4684
Size: 1398 2976 6665
Fragments arranged by size:
6665 2976 1398
FspI TGC'GCA
Cuts at: 8820 8820
Size: 11039
HaeII r_GCGC'y
Cuts at: 5073 8473 8721 9951 10722 5073
Size: 3400 248 1230 771 5390
Fragments arranged by size:
5390 3400 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 463 495 868 900 925 957 2056 2088 2803
Size: 32 373 32 25 32 1099 32 715
Cuts at: 2803 2835 3026 3058 3622 3654 4018 4050 4186
Size: 32 191 32 564 32 364 32 136
Cuts at: 4186 4218 4936 4938 4968 4970 4981 5013 5414
Size: 32 718 2 30 2 11 32 401
Cuts at: 5414 5446 5869 5901 6286 6318 7446 7478 8881
Size: 32 423 32 385 32 1128 32 1403
Cuts at: 8881 8911 8913 8943 463
Size: 30 2 30 2559
Fragments arranged by size:
2559 1403 1128 1099 718 715 564 423
401 385 373 364 191 136 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 30 25
11 2 2 2
HincII GTy'rAC
Cuts at: 5327 8078 5327
Size: 2751 8288
HindIII A'AGCT_T
Cuts at: 5725 5725
Size: 11039
HpaI GTT'AAC
Cuts at: 5327 5327
Size: 11039
KasI G'GCGC_C
Cuts at: 5069 8469 8717 5069
Size: 3400 248 7391
Fragments arranged by size:
7391 3400 248
KpnI G_GTAC'C
Cuts at: 8059 8059
Size: 11039
MfeI C'AATT_G
Cuts at: 5391 7295 5391
Size: 1904 9135
MluI A'CGCG_T
Cuts at: 10472 10472
Size: 11039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1749 5765 6367 7273 7690 9798 9982 1749
Size: 4016 602 906 417 2108 184 2806
Fragments arranged by size:
4016 2806 2108 906 602 417 184
MscI TGG'CCA
Cuts at: 5133 6367 8800 5133
Size: 1234 2433 7372
Fragments arranged by size:
7372 2433 1234
MslI CAynn'nnrTG
Cuts at: 1388 2392 2708 3165 3443 3629 3845 4482 4757
Size: 1004 316 457 278 186 216 637 275
Cuts at: 4757 5231 9028 9155 1388
Size: 474 3797 127 3272
Fragments arranged by size:
3797 3272 1004 637 474 457 316 278
275 216 186 127
MspA1I CmG'CkG
Cuts at: 117 421 5231 6138 8150 8464 8824 9608 9853
Size: 304 4810 907 2012 314 360 784 245
Cuts at: 9853 117
Size: 1303
Fragments arranged by size:
4810 2012 1303 907 784 360 314 304
245
NaeI GCC'GGC
Cuts at: 7121 9220 7121
Size: 2099 8940
NarI GG'CG_CC
Cuts at: 5070 8470 8718 5070
Size: 3400 248 7391
Fragments arranged by size:
7391 3400 248
NcoI C'CATG_G
Cuts at: 3131 3344 3131
Size: 213 10826
NdeI CA'TA_TG
Cuts at: 7671 7671
Size: 11039
NgoMIV G'CCGG_C
Cuts at: 7119 9218 7119
Size: 2099 8940
NsiI A_TGCA'T
Cuts at: 2251 3162 2251
Size: 911 10128
NspI r_CATG'y
Cuts at: 2249 2253 2319 2654 2878 3076 3844 4655 4756
Size: 4 66 335 224 198 768 811 101
Cuts at: 4756 7027 7247 7986 8092 8592 10195 2249
Size: 2271 220 739 106 500 1603 3093
Fragments arranged by size:
3093 2271 1603 811 768 739 500 335
224 220 198 106 101 66 4
PciI A'CATG_T
Cuts at: 3072 3840 7023 7243 10191 3072
Size: 768 3183 220 2948 3920
Fragments arranged by size:
3920 3183 2948 768 220
PflMI CCAn_nnn'nTGG
Cuts at: 7062 7062
Size: 11039
PfoI T'CCnGG_A
Cuts at: 4047 6377 4047
Size: 2330 8709
PmeI GTTT'AAAC
Cuts at: 8038 8038
Size: 11039
PmlI CAC'GTG
Cuts at: 3997 3997
Size: 11039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5611 5643 9485 9517 5611
Size: 32 3842 32 7133
Fragments arranged by size:
7133 3842 32 32
PpuMI rG'GwC_Cy
Cuts at: 4473 7926 4473
Size: 3453 7586
PshAI GACnn'nnGTC
Cuts at: 1731 5839 1731
Size: 4108 6931
PsiI TTA'TAA
Cuts at: 838 1772 2029 5743 5992 6812 838
Size: 934 257 3714 249 820 5065
Fragments arranged by size:
5065 3714 934 820 257 249
PspOMI G'GGCC_C
Cuts at: 4444 4444
Size: 11039
PspXI vC'TCGA_Gb
Cuts at: 4880 4880
Size: 11039
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2670 2702 2841 2873 2670
Size: 32 139 32 10836
Fragments arranged by size:
10836 139 32 32
PstI C_TGCA'G
Cuts at: 122 489 8086 8771 122
Size: 367 7597 685 2390
Fragments arranged by size:
7597 2390 685 367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11039
PvuII CAG'CTG
Cuts at: 117 421 5231 6138 8464 8824 117
Size: 304 4810 907 2326 360 2332
Fragments arranged by size:
4810 2332 2326 907 360 304
RsrII CG'GwC_CG
Cuts at: 9234 9234
Size: 11039
SacI G_AGCT'C
Cuts at: 1171 1420 3872 4638 8053 1171
Size: 249 2452 766 3415 4157
Fragments arranged by size:
4157 3415 2452 766 249
SalI G'TCGA_C
Cuts at: 8076 8076
Size: 11039
SanDI GG'GwC_CC
Cuts at: 4473 4473
Size: 11039
SapI GCTCTTCn'nnn_
Cuts at: 3234 3701 3860 9062 9272 3234
Size: 467 159 5202 210 5001
Fragments arranged by size:
5202 5001 467 210 159
SbfI CC_TGCA'GG
Cuts at: 8086 8086
Size: 11039
ScaI AGT'ACT
Cuts at: 5801 7198 7909 10556 5801
Size: 1397 711 2647 6284
Fragments arranged by size:
6284 2647 1397 711
SexAI A'CCwGG_T
Cuts at: 5540 6846 5540
Size: 1306 9733
SfcI C'TryA_G
Cuts at: 33 118 485 653 3203 6089 6692 6980 8082
Size: 85 367 168 2550 2886 603 288 1102
Cuts at: 8082 8767 9735 9926 33
Size: 685 968 191 1146
Fragments arranged by size:
2886 2550 1146 1102 968 685 603 367
288 191 168 85
SfoI GGC'GCC
Cuts at: 5071 8471 8719 5071
Size: 3400 248 7391
Fragments arranged by size:
7391 3400 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11039
SmaI CCC'GGG
Cuts at: 8061 8061
Size: 11039
SmlI C'TyrA_G
Cuts at: 1515 2993 3896 4076 4637 4797 4880 7214 7535
Size: 1478 903 180 561 160 83 2334 321
Cuts at: 7535 7633 8194 9109 9823 10085 1515
Size: 98 561 915 714 262 2469
Fragments arranged by size:
2469 2334 1478 915 903 714 561 561
321 262 180 160 98 83
SpeI A'CTAG_T
Cuts at: 67 2683 67
Size: 2616 8423
SphI G_CATG'C
Cuts at: 2249 2253 2319 2878 4756 8092 8592 2249
Size: 4 66 559 1878 3336 500 4696
Fragments arranged by size:
4696 3336 1878 559 500 66 4
SspI AAT'ATT
Cuts at: 917 1237 2277 2541 4843 917
Size: 320 1040 264 2302 7113
Fragments arranged by size:
7113 2302 1040 320 264
StuI AGG'CCT
Cuts at: 6865 7693 6865
Size: 828 10211
StyI C'CwwG_G
Cuts at: 3131 3344 6965 7961 8173 8527 3131
Size: 213 3621 996 212 354 5643
Fragments arranged by size:
5643 3621 996 354 213 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3671 6930 8622 8755 9214 9423 3671
Size: 3259 1692 133 459 209 5287
Fragments arranged by size:
5287 3259 1692 459 209 133
TatI w'GTAC_w
Cuts at: 1831 2491 3616 4609 5799 7083 7185 7196 7299
Size: 660 1125 993 1190 1284 102 11 103
Cuts at: 7299 7907 7967 7979 10554 1831
Size: 608 60 12 2575 2316
Fragments arranged by size:
2575 2316 1284 1190 1125 993 660 608
103 102 60 12 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 3059 3091 4479 4511 5617 5649 5901
Size: 32 262 32 1388 32 1106 32 252
Cuts at: 5901 5933 5940 5972 6916 6948 7078 7110 7344
Size: 32 7 32 944 32 130 32 234
Cuts at: 7344 7376 7918 7950 10556 10588 2765
Size: 32 542 32 2606 32 3216
Fragments arranged by size:
3216 2606 1388 1106 944 542 262 252
234 130 32 32 32 32 32 32
32 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 4950 8836 4950
Size: 3886 7153
XbaI T'CTAG_A
Cuts at: 1345 8070 1345
Size: 6725 4314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 573 3752 6375 6918 8534 573
Size: 3179 2623 543 1616 3078
Fragments arranged by size:
3179 3078 2623 1616 543
XhoI C'TCGA_G
Cuts at: 4880 7535 4880
Size: 2655 8384
XmaI C'CCGG_G
Cuts at: 8059 8059
Size: 11039
XmnI GAAnn'nnTTC
Cuts at: 980 5374 980
Size: 4394 6645
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AscI AvrII BcgI BglI
BmgBI BmtI BsiWI BssHII BstZ17I CspCI DraIII FseI
FspAI NheI NotI NruI PacI PasI SacII SfiI
SgrAI SnaBI SrfI SwaI ZraI