Restriction map for
pF1KA1717
(Circular) (Six-base) MAPSORT of: pF1KA1717.seq Check: 8654 from: 1 to: 4211
pF1KA1717 4211 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 368 694 1249 368
Size: 326 555 3330
Fragments arranged by size:
3330 555 326
Acc65I G'GTAC_C
Cuts at: 1163 1227 1163
Size: 64 4147
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1169 1198 2036 2468 3215 3483 653
Size: 516 29 838 432 747 268 1381
Fragments arranged by size:
1381 838 747 516 432 268 29
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 892 924 892
Size: 32 4179
AlwNI CAG_nnn'CTG
Cuts at: 884 1516 3083 884
Size: 632 1567 2012
Fragments arranged by size:
2012 1567 632
ApaI G_GGCC'C
Cuts at: 891 1052 891
Size: 161 4050
ApaLI G'TGCA_C
Cuts at: 2981 2981
Size: 4211
ApoI r'AATT_y
Cuts at: 580 669 1215 3564 3820 580
Size: 89 546 2349 256 971
Fragments arranged by size:
2349 971 546 256 89
AseI AT'TA_AT
Cuts at: 20 20
Size: 4211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 1132 1231 1132
Size: 99 4112
BamHI G'GATC_C
Cuts at: 1236 1236
Size: 4211
BanI G'GyrC_C
Cuts at: 149 1163 1227 1641 1889 1924 149
Size: 1014 64 414 248 35 2436
Fragments arranged by size:
2436 1014 414 248 64 35
BanII G_rGCy'C
Cuts at: 891 947 1052 1100 1225 2255 891
Size: 56 105 48 125 1030 2847
Fragments arranged by size:
2847 1030 125 105 56 48
BbeI G_GCGC'C
Cuts at: 1645 1893 1645
Size: 248 3963
BciVI GTATCCnnnnn_n'
Cuts at: 969 2109 2870 4211 969
Size: 1140 761 1341 969
Fragments arranged by size:
1341 1140 969 761
BclI T'GATC_A
Cuts at: 1731 1731
Size: 4211
BfrBI ATG'CAT
Cuts at: 1061 1061
Size: 4211
BglI GCCn_nnn'nGGC
Cuts at: 1189 1189
Size: 4211
BglII A'GATC_T
Cuts at: 1726 3763 1726
Size: 2037 2174
BlpI GC'TnA_GC
Cuts at: 1323 1323
Size: 4211
Bme1580I G_kGCm'C
Cuts at: 152 361 891 1052 1836 1929 2985 152
Size: 209 530 161 784 93 1056 1378
Fragments arranged by size:
1378 1056 784 530 209 161 93
BmrI ACTGGGnnnn_n'
Cuts at: 894 1423 1504 1839 3983 894
Size: 529 81 335 2144 1122
Fragments arranged by size:
2144 1122 529 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 337 548 806 304
Size: 33 211 258 3709
Fragments arranged by size:
3709 258 211 33
Bpu10I CC'TnA_GC
Cuts at: 941 941
Size: 4211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 2266 2758 3056 1387
Size: 879 492 298 2542
Fragments arranged by size:
2542 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3915 3915
Size: 4211
BsaAI yAC'GTr
Cuts at: 362 957 1564 362
Size: 595 607 3009
Fragments arranged by size:
3009 607 595
BsaBI GATnn'nnATC
Cuts at: 6 1725 3762 6
Size: 1719 2037 455
Fragments arranged by size:
2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 1642 1890 2592 4088 1642
Size: 248 702 1496 1765
Fragments arranged by size:
1765 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1400 1921 2599 2873 3020 3431 1400
Size: 521 678 274 147 411 2180
Fragments arranged by size:
2180 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 892 922 958 988 892
Size: 30 36 30 4115
Fragments arranged by size:
4115 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 189 148
Size: 41 4170
BseYI C'CCAG_C
Cuts at: 1196 1636 2394 2971 3467 1196
Size: 440 758 577 496 1940
Fragments arranged by size:
1940 758 577 496 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 168
Size: 4211
BsiEI CG_ry'CG
Cuts at: 104 1753 1799 3007 104
Size: 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1100 1225 2003 2193 2985 1100
Size: 125 778 190 792 2326
Fragments arranged by size:
2326 792 778 190 125
Bsp1286I G_dGCh'C
Cuts at: 152 361 891 947 1052 1100 1225 1836 1929
Size: 209 530 56 105 48 125 611 93
Cuts at: 1929 2003 2193 2255 2985 152
Size: 74 190 62 730 1378
Fragments arranged by size:
1378 730 611 530 209 190 125 105
93 74 62 56 48
BspEI T'CCGG_A
Cuts at: 3431 3431
Size: 4211
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1261 1475 1777 2158 1261
Size: 214 302 381 3314
Fragments arranged by size:
3314 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2503 2557 4041 4206 50
Size: 2453 54 1484 165 55
Fragments arranged by size:
2453 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2123 2123
Size: 4211
BsrFI r'CCGG_y
Cuts at: 2209 2390 2599 2209
Size: 181 209 3821
Fragments arranged by size:
3821 209 181
BssSI C'ACGA_G
Cuts at: 2482 2840 2482
Size: 358 3853
BstAPI GCAn_nnn'nTGC
Cuts at: 1588 1588
Size: 4211
BstBI TT'CG_AA
Cuts at: 852 2572 852
Size: 1720 2491
BstYI r'GATC_y
Cuts at: 1 732 1236 1703 1726 2061 2307 3319 3405
Size: 731 504 467 23 335 246 1012 86
Cuts at: 3405 3417 3763 1
Size: 12 346 449
Fragments arranged by size:
1012 731 504 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2161 2342 2505 2161
Size: 181 163 3867
Fragments arranged by size:
3867 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1454 1085
Size: 369 3842
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2775 1917
Size: 858 3353
EaeI y'GGCC_r
Cuts at: 1796 1970 2361 2388 3733 1796
Size: 174 391 27 1345 2274
Fragments arranged by size:
2274 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1796 1796
Size: 4211
EarI CTCTTCn'nnn_
Cuts at: 728 1088 1132 2234 2444 728
Size: 360 44 1102 210 2495
Fragments arranged by size:
2495 1102 360 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2419 2729 2875 4021 2419
Size: 310 146 1146 2609
Fragments arranged by size:
2609 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1098 1223 1098
Size: 125 4086
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 337 548 653 806 1169 1198 2036 2468
Size: 33 211 105 153 363 29 838 432
Cuts at: 2468 3215 3483 304
Size: 747 268 1032
Fragments arranged by size:
1032 838 747 432 363 268 211 153
105 33 29
EcoO109I rG'GnC_Cy
Cuts at: 817 888 1350 817
Size: 71 462 3678
Fragments arranged by size:
3678 462 71
EcoRI G'AATT_C
Cuts at: 1215 1215
Size: 4211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 279 2230 279
Size: 1951 2260
FspI TGC'GCA
Cuts at: 1992 1992
Size: 4211
HaeII r_GCGC'y
Cuts at: 1645 1893 2915 3894 1645
Size: 248 1022 979 1962
Fragments arranged by size:
1962 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2053 2083 2085 2115 2053
Size: 30 2 30 4149
Fragments arranged by size:
4149 30 30 2
HincII GTy'rAC
Cuts at: 695 873 1250 695
Size: 178 377 3656
Fragments arranged by size:
3656 377 178
KasI G'GCGC_C
Cuts at: 1641 1889 1641
Size: 248 3963
KpnI G_GTAC'C
Cuts at: 1167 1231 1167
Size: 64 4147
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2882 3066 2882
Size: 184 4027
MscI TGG'CCA
Cuts at: 1972 1972
Size: 4211
MslI CAynn'nnrTG
Cuts at: 934 2200 2327 934
Size: 1266 127 2818
Fragments arranged by size:
2818 1266 127
MspA1I CmG'CkG
Cuts at: 1322 1636 1996 3009 3254 1322
Size: 314 360 1013 245 2279
Fragments arranged by size:
2279 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2392 2392
Size: 4211
NarI GG'CG_CC
Cuts at: 1642 1890 1642
Size: 248 3963
NcoI C'CATG_G
Cuts at: 107 107
Size: 4211
NgoMIV G'CCGG_C
Cuts at: 2390 2390
Size: 4211
NsiI A_TGCA'T
Cuts at: 1063 1063
Size: 4211
NspI r_CATG'y
Cuts at: 1264 1764 2671 1264
Size: 500 907 2804
Fragments arranged by size:
2804 907 500
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 892 924 3350 3382 892
Size: 32 2426 32 1721
Fragments arranged by size:
2426 1721 32 32
PpuMI rG'GwC_Cy
Cuts at: 817 817
Size: 4211
PsiI TTA'TAA
Cuts at: 842 842
Size: 4211
PspOMI G'GGCC_C
Cuts at: 887 1048 887
Size: 161 4050
PstI C_TGCA'G
Cuts at: 1258 1943 1258
Size: 685 3526
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 1636 1996 1636
Size: 360 3851
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1100 1225 1100
Size: 125 4086
SalI G'TCGA_C
Cuts at: 693 1248 693
Size: 555 3656
SapI GCTCTTCn'nnn_
Cuts at: 728 1088 2234 2444 728
Size: 360 1146 210 2495
Fragments arranged by size:
2495 1146 360 210
SbfI CC_TGCA'GG
Cuts at: 1258 1258
Size: 4211
ScaI AGT'ACT
Cuts at: 967 3728 967
Size: 2761 1450
SexAI A'CCwGG_T
Cuts at: 1166 1166
Size: 4211
SfcI C'TryA_G
Cuts at: 33 1254 1939 2932 3123 33
Size: 1221 685 993 191 1121
Fragments arranged by size:
1221 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1189 1189
Size: 4211
SfoI GGC'GCC
Cuts at: 1643 1891 1643
Size: 248 3963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 1134 1233 1134
Size: 99 4112
SmlI C'TyrA_G
Cuts at: 1366 2281 2773 3035 1366
Size: 915 492 262 2542
Fragments arranged by size:
2542 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 1264 1764 1264
Size: 500 3711
StuI AGG'CCT
Cuts at: 645 1183 645
Size: 538 3673
StyI C'CwwG_G
Cuts at: 107 977 1345 1699 107
Size: 870 368 354 2619
Fragments arranged by size:
2619 870 368 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1210 1794 1927 2386 2595 1210
Size: 584 133 459 209 2826
Fragments arranged by size:
2826 584 459 209 133
TatI w'GTAC_w
Cuts at: 965 3726 965
Size: 2761 1450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 659 691 3728 3760 659
Size: 32 3037 32 1110
Fragments arranged by size:
3037 1110 32 32
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1042 1706 1042
Size: 664 3547
XmaI C'CCGG_G
Cuts at: 1132 1231 1132
Size: 99 4112
XmnI GAAnn'nnTTC
Cuts at: 259 1183 259
Size: 924 3287
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI