Restriction map for
pF1KA1707
(Circular) (Six-base) MAPSORT of: pF1KA1707.seq Check: 7288 from: 1 to: 6164
pF1KA1707 6164 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 3202 3202
Size: 6164
Acc65I G'GTAC_C
Cuts at: 3180 3180
Size: 6164
AclI AA'CG_TT
Cuts at: 6004 6004
Size: 6164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 1088 1772 2105 3989 4421 4774 5436 821
Size: 267 684 333 1884 432 353 662 1549
Fragments arranged by size:
1884 1549 684 662 432 353 333 267
AflII C'TTAA_G
Cuts at: 1381 2362 1381
Size: 981 5183
AflIII A'CryG_T
Cuts at: 442 1468 3516 5316 5597 442
Size: 1026 2048 1800 281 1009
Fragments arranged by size:
2048 1800 1026 1009 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1186 1218 1186
Size: 32 6132
AlwNI CAG_nnn'CTG
Cuts at: 3469 4907 3469
Size: 1438 4726
ApaLI G'TGCA_C
Cuts at: 5002 5002
Size: 6164
ApoI r'AATT_y
Cuts at: 157 343 1376 1858 2862 3168 5517 5773 157
Size: 186 1033 482 1004 306 2349 256 548
Fragments arranged by size:
2349 1033 1004 548 482 306 256 186
AseI AT'TA_AT
Cuts at: 20 20
Size: 6164
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
AvaI C'yCGr_G
Cuts at: 3184 3184
Size: 6164
BamHI G'GATC_C
Cuts at: 1053 3024 3189 1053
Size: 1971 165 4028
Fragments arranged by size:
4028 1971 165
BanI G'GyrC_C
Cuts at: 3180 3594 3842 3877 3180
Size: 414 248 35 5467
Fragments arranged by size:
5467 414 248 35
BanII G_rGCy'C
Cuts at: 691 3116 3178 4208 691
Size: 2425 62 1030 2647
Fragments arranged by size:
2647 2425 1030 62
BbeI G_GCGC'C
Cuts at: 3598 3846 3598
Size: 248 5916
BbsI GAAGACnn'nnnn_
Cuts at: 809 916 2508 809
Size: 107 1592 4465
Fragments arranged by size:
4465 1592 107
BciVI GTATCCnnnnn_n'
Cuts at: 485 1689 2667 4062 5118 6164 485
Size: 1204 978 1395 1056 1046 485
Fragments arranged by size:
1395 1204 1056 1046 978 485
BclI T'GATC_A
Cuts at: 831 3684 831
Size: 2853 3311
BglII A'GATC_T
Cuts at: 2304 3679 5716 2304
Size: 1375 2037 2752
Fragments arranged by size:
2752 2037 1375
BlpI GC'TnA_GC
Cuts at: 2675 3276 2675
Size: 601 5563
Bme1580I G_kGCm'C
Cuts at: 3789 3882 5006 3789
Size: 93 1124 4947
Fragments arranged by size:
4947 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3376 3457 3792 5936 3376
Size: 81 335 2144 3604
Fragments arranged by size:
3604 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4531 4531
Size: 6164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2329 2329
Size: 6164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3340 4219 4933 5231 3340
Size: 879 714 298 4273
Fragments arranged by size:
4273 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1788 5868 1788
Size: 4080 2084
BsaAI yAC'GTr
Cuts at: 2719 3517 2719
Size: 798 5366
BsaBI GATnn'nnATC
Cuts at: 6 1742 2711 3678 5715 6
Size: 1736 969 967 2037 455
Fragments arranged by size:
2037 1736 969 967 455
BsaHI Gr'CG_yC
Cuts at: 3595 3843 4545 6041 3595
Size: 248 702 1496 3718
Fragments arranged by size:
3718 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3353 3874 4552 4963 5110 5384 3353
Size: 521 678 411 147 274 4133
Fragments arranged by size:
4133 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3052 3082 3052
Size: 30 6134
BseYI C'CCAG_C
Cuts at: 2660 3589 4347 5012 5420 2660
Size: 929 758 665 408 3404
Fragments arranged by size:
3404 929 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 2765
Size: 6164
BsiEI CG_ry'CG
Cuts at: 104 3706 3752 4982 104
Size: 3602 46 1230 1286
Fragments arranged by size:
3602 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3116 3178 3956 4146 5006 3116
Size: 62 778 190 860 4274
Fragments arranged by size:
4274 860 778 190 62
BsmBI CGTCTCn'nnnn_
Cuts at: 2588 2588
Size: 6164
Bsp1286I G_dGCh'C
Cuts at: 691 3116 3178 3789 3882 3956 4146 4208 5006
Size: 2425 62 611 93 74 190 62 798
Cuts at: 5006 691
Size: 1849
Fragments arranged by size:
2425 1849 798 611 190 93 74 62
62
BspHI T'CATG_A
Cuts at: 4596 4596
Size: 6164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3214 3428 3730 4111 3214
Size: 214 302 381 5267
Fragments arranged by size:
5267 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4456 4510 5994 6159 50
Size: 4406 54 1484 165 55
Fragments arranged by size:
4406 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 409 1684 4076 409
Size: 1275 2392 2497
Fragments arranged by size:
2497 2392 1275
BsrFI r'CCGG_y
Cuts at: 1168 4162 4343 1168
Size: 2994 181 2989
Fragments arranged by size:
2994 2989 181
BsrGI T'GTAC_A
Cuts at: 1479 2844 1479
Size: 1365 4799
BssSI C'ACGA_G
Cuts at: 4435 5143 4435
Size: 708 5456
BstAPI GCAn_nnn'nTGC
Cuts at: 3541 3541
Size: 6164
BstBI TT'CG_AA
Cuts at: 4525 4525
Size: 6164
BstYI r'GATC_y
Cuts at: 1 1053 1557 1652 2304 2940 3024 3189 3656
Size: 1052 504 95 652 636 84 165 467
Cuts at: 3656 3679 4014 4260 4566 4578 4664 5716 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1052 652 636 504 467 449 335
306 246 165 95 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 208 208
Size: 6164
BtgI C'CryG_G
Cuts at: 2614 2614
Size: 6164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4114 4295 4458 4114
Size: 181 163 5820
Fragments arranged by size:
5820 181 163
BtsI GCAGTG_nn'
Cuts at: 199 2982 3407 199
Size: 2783 425 2956
Fragments arranged by size:
2956 2783 425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1005 1040 2918 2953 1005
Size: 35 1878 35 4216
Fragments arranged by size:
4216 1878 35 35
DraI TTT'AAA
Cuts at: 1709 3163 1709
Size: 1454 4710
DrdI GACnn_nn'nnGTC
Cuts at: 3870 5214 3870
Size: 1344 4820
EaeI y'GGCC_r
Cuts at: 2132 3749 3923 4314 4341 5686 2132
Size: 1617 174 391 27 1345 2610
Fragments arranged by size:
2610 1617 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3749 3749
Size: 6164
EarI CTCTTCn'nnn_
Cuts at: 467 1791 2016 4187 4397 467
Size: 1324 225 2171 210 2234
Fragments arranged by size:
2234 2171 1324 225 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4372 5114 5260 5974 4372
Size: 742 146 714 4562
Fragments arranged by size:
4562 742 714 146
EcoICRI GAG'CTC
Cuts at: 3114 3176 3114
Size: 62 6102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 1088 1772 2105 2329 3989 4421 4774 5436
Size: 267 684 333 224 1660 432 353 662
Cuts at: 5436 821
Size: 1549
Fragments arranged by size:
1660 1549 684 662 432 353 333 267
224
EcoO109I rG'GnC_Cy
Cuts at: 551 1351 3303 551
Size: 800 1952 3412
Fragments arranged by size:
3412 1952 800
EcoRI G'AATT_C
Cuts at: 1858 3168 1858
Size: 1310 4854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4183 4183
Size: 6164
FspI TGC'GCA
Cuts at: 737 3945 737
Size: 3208 2956
HaeII r_GCGC'y
Cuts at: 3598 3846 5076 5847 3598
Size: 248 1230 771 3915
Fragments arranged by size:
3915 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2244 2276 2371 2403 3101 3133 4006 4036 4038
Size: 32 95 32 698 32 873 30 2
Cuts at: 4038 4068 2244
Size: 30 4340
Fragments arranged by size:
4340 873 698 95 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 311 429 974 1136 3036 3203 311
Size: 118 545 162 1900 167 3272
Fragments arranged by size:
3272 1900 545 167 162 118
HindIII A'AGCT_T
Cuts at: 3012 3012
Size: 6164
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 6164
KasI G'GCGC_C
Cuts at: 3594 3842 3594
Size: 248 5916
KpnI G_GTAC'C
Cuts at: 3184 3184
Size: 6164
MfeI C'AATT_G
Cuts at: 1325 1325
Size: 6164
MluI A'CGCG_T
Cuts at: 5597 5597
Size: 6164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4923 5107 4923
Size: 184 5980
MscI TGG'CCA
Cuts at: 3925 3925
Size: 6164
MslI CAynn'nnrTG
Cuts at: 4153 4280 4153
Size: 127 6037
MspA1I CmG'CkG
Cuts at: 1318 3275 3589 3949 4733 4978 1318
Size: 1957 314 360 784 245 2504
Fragments arranged by size:
2504 1957 784 360 314 245
NaeI GCC'GGC
Cuts at: 4345 4345
Size: 6164
NarI GG'CG_CC
Cuts at: 3595 3843 3595
Size: 248 5916
NcoI C'CATG_G
Cuts at: 2614 2614
Size: 6164
NgoMIV G'CCGG_C
Cuts at: 4343 4343
Size: 6164
NspI r_CATG'y
Cuts at: 446 1472 3217 3717 5320 446
Size: 1026 1745 500 1603 1290
Fragments arranged by size:
1745 1603 1290 1026 500
PciI A'CATG_T
Cuts at: 442 1468 5316 442
Size: 1026 3848 1290
Fragments arranged by size:
3848 1290 1026
PfoI T'CCnGG_A
Cuts at: 904 904
Size: 6164
PmeI GTTT'AAAC
Cuts at: 3163 3163
Size: 6164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2511 2543 4610 4642 2511
Size: 32 2067 32 4033
Fragments arranged by size:
4033 2067 32 32
PpuMI rG'GwC_Cy
Cuts at: 551 1351 551
Size: 800 5364
PshAI GACnn'nnGTC
Cuts at: 316 514 316
Size: 198 5966
PstI C_TGCA'G
Cuts at: 2159 3211 3896 2159
Size: 1052 685 4427
Fragments arranged by size:
4427 1052 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6164
PvuII CAG'CTG
Cuts at: 1318 3589 3949 1318
Size: 2271 360 3533
Fragments arranged by size:
3533 2271 360
RsrII CG'GwC_CG
Cuts at: 4359 4359
Size: 6164
SacI G_AGCT'C
Cuts at: 3116 3178 3116
Size: 62 6102
SalI G'TCGA_C
Cuts at: 3201 3201
Size: 6164
SapI GCTCTTCn'nnn_
Cuts at: 4187 4397 4187
Size: 210 5954
SbfI CC_TGCA'GG
Cuts at: 3211 3211
Size: 6164
ScaI AGT'ACT
Cuts at: 1431 5681 1431
Size: 4250 1914
SfcI C'TryA_G
Cuts at: 33 2155 3207 3892 4860 5051 33
Size: 2122 1052 685 968 191 1146
Fragments arranged by size:
2122 1146 1052 968 685 191
SfoI GGC'GCC
Cuts at: 3596 3844 3596
Size: 248 5916
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6164
SmaI CCC'GGG
Cuts at: 3186 3186
Size: 6164
SmlI C'TyrA_G
Cuts at: 1381 2362 3319 4234 4948 5210 1381
Size: 981 957 915 714 262 2335
Fragments arranged by size:
2335 981 957 915 714 262
SnaBI TAC'GTA
Cuts at: 2719 2719
Size: 6164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6164
SphI G_CATG'C
Cuts at: 3217 3717 3217
Size: 500 5664
SspI AAT'ATT
Cuts at: 871 952 871
Size: 81 6083
StyI C'CwwG_G
Cuts at: 2614 3298 3652 2614
Size: 684 354 5126
Fragments arranged by size:
5126 684 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3747 3880 4339 4548 3747
Size: 133 459 209 5363
Fragments arranged by size:
5363 459 209 133
TatI w'GTAC_w
Cuts at: 955 1429 1479 2844 5679 955
Size: 474 50 1365 2835 1440
Fragments arranged by size:
2835 1440 1365 474 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5681 5713 5681
Size: 32 6132
Tth111I GACn'n_nGTC
Cuts at: 2733 3961 2733
Size: 1228 4936
XbaI T'CTAG_A
Cuts at: 228 3195 228
Size: 2967 3197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1212 2210 3659 1212
Size: 998 1449 3717
Fragments arranged by size:
3717 1449 998
XmaI C'CCGG_G
Cuts at: 3184 3184
Size: 6164
XmnI GAAnn'nnTTC
Cuts at: 461 2642 3104 461
Size: 2181 462 3521
Fragments arranged by size:
3521 2181 462
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BspEI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI