Restriction map for
pF1KA1689
(Circular) (Six-base) MAPSORT of: pF1KA1689.seq Check: 5564 from: 1 to: 10985
pF1KA1689 10985 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1478 4883 6853 8023 1478
Size: 3405 1970 1170 4440
Fragments arranged by size:
4440 3405 1970 1170
Acc65I G'GTAC_C
Cuts at: 2688 6037 8001 2688
Size: 3349 1964 5672
Fragments arranged by size:
5672 3349 1964
AclI AA'CG_TT
Cuts at: 7263 10825 7263
Size: 3562 7423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 815 1204 1256 1769 1784 2036 4013 4122
Size: 387 389 52 513 15 252 1977 109
Cuts at: 4122 4233 4819 5441 5981 6632 7106 8810 9242
Size: 111 586 622 540 651 474 1704 432
Cuts at: 9242 9595 10257 428
Size: 353 662 1156
Fragments arranged by size:
1977 1704 1156 662 651 622 586 540
513 474 432 389 387 353 252 111
109 52 15
AflIII A'CryG_T
Cuts at: 6707 8337 10137 10418 6707
Size: 1630 1800 281 7274
Fragments arranged by size:
7274 1800 1630 281
AleI CACnn'nnGTG
Cuts at: 262 5950 7306 262
Size: 5688 1356 3941
Fragments arranged by size:
5688 3941 1356
AlwNI CAG_nnn'CTG
Cuts at: 558 2140 7933 8290 9728 558
Size: 1582 5793 357 1438 1815
Fragments arranged by size:
5793 1815 1582 1438 357
ApaI G_GGCC'C
Cuts at: 6749 7908 6749
Size: 1159 9826
ApaLI G'TGCA_C
Cuts at: 7309 9823 7309
Size: 2514 8471
ApoI r'AATT_y
Cuts at: 1126 1379 2034 2276 2585 3054 3806 3972 4221
Size: 253 655 242 309 469 752 166 249
Cuts at: 4221 4686 4971 5149 5169 5982 6783 7006 7989
Size: 465 285 178 20 813 801 223 983
Cuts at: 7989 10338 10594 1126
Size: 2349 256 1517
Fragments arranged by size:
2349 1517 983 813 801 752 655 469
465 309 285 256 253 249 242 223
178 166 20
AseI AT'TA_AT
Cuts at: 20 470 2702 4346 20
Size: 450 2232 1644 6659
Fragments arranged by size:
6659 2232 1644 450
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10985
AvaI C'yCGr_G
Cuts at: 845 8005 845
Size: 7160 3825
AvrII C'CTAG_G
Cuts at: 310 310
Size: 10985
BamHI G'GATC_C
Cuts at: 8010 8010
Size: 10985
BanI G'GyrC_C
Cuts at: 160 2688 6037 8001 8415 8663 8698 160
Size: 2528 3349 1964 414 248 35 2447
Fragments arranged by size:
3349 2528 2447 1964 414 248 35
BanII G_rGCy'C
Cuts at: 172 257 296 2456 6694 6749 6800 7908 7999
Size: 85 39 2160 4238 55 51 1108 91
Cuts at: 7999 9029 172
Size: 1030 2128
Fragments arranged by size:
4238 2160 2128 1108 1030 91 85 55
51 39
BbeI G_GCGC'C
Cuts at: 164 8419 8667 164
Size: 8255 248 2482
Fragments arranged by size:
8255 2482 248
BbsI GAAGACnn'nnnn_
Cuts at: 1775 2854 3019 3184 3340 3502 3667 6439 7574
Size: 1079 165 165 156 162 165 2772 1135
Cuts at: 7574 7620 1775
Size: 46 5140
Fragments arranged by size:
5140 2772 1135 1079 165 165 165 162
156 46
BbvCI CC'TCA_GC
Cuts at: 1856 3686 1856
Size: 1830 9155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1189 1223 1189
Size: 34 10951
BciVI GTATCCnnnnn_n'
Cuts at: 3249 8883 9939 10985 3249
Size: 5634 1056 1046 3249
Fragments arranged by size:
5634 3249 1056 1046
BclI T'GATC_A
Cuts at: 1914 7860 8505 1914
Size: 5946 645 4394
Fragments arranged by size:
5946 4394 645
BfrBI ATG'CAT
Cuts at: 4197 5915 7468 7472 4197
Size: 1718 1553 4 7710
Fragments arranged by size:
7710 1718 1553 4
BglII A'GATC_T
Cuts at: 364 1396 3537 3998 5833 6087 7456 7738 8500
Size: 1032 2141 461 1835 254 1369 282 762
Cuts at: 8500 10537 364
Size: 2037 812
Fragments arranged by size:
2141 2037 1835 1369 1032 812 762 461
282 254
BlpI GC'TnA_GC
Cuts at: 5162 5309 6410 7839 8097 5162
Size: 147 1101 1429 258 8050
Fragments arranged by size:
8050 1429 1101 258 147
Bme1580I G_kGCm'C
Cuts at: 123 269 6749 7313 7896 7908 8610 8703 9827
Size: 146 6480 564 583 12 702 93 1124
Cuts at: 9827 123
Size: 1281
Fragments arranged by size:
6480 1281 1124 702 583 564 146 93
12
BmrI ACTGGGnnnn_n'
Cuts at: 1540 8197 8278 8613 10757 1540
Size: 6657 81 335 2144 1768
Fragments arranged by size:
6657 2144 1768 335 81
BmtI G_CTAG'C
Cuts at: 3832 3832
Size: 10985
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3666 7329 9352 3666
Size: 3663 2023 5299
Fragments arranged by size:
5299 3663 2023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 966 4301 5347 5792 6707 7388 734
Size: 232 3335 1046 445 915 681 4331
Fragments arranged by size:
4331 3335 1046 915 681 445 232
Bpu10I CC'TnA_GC
Cuts at: 127 1622 1856 3686 127
Size: 1495 234 1830 7426
Fragments arranged by size:
7426 1830 1495 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3636 4077 4352 6448 6887 6923 7895 8161 9040
Size: 441 275 2096 439 36 972 266 879
Cuts at: 9040 9754 10052 3636
Size: 714 298 4569
Fragments arranged by size:
4569 2096 972 879 714 441 439 298
275 266 36
BsaI GGTCTCn'nnnn_
Cuts at: 4075 4833 4839 5833 10689 4075
Size: 758 6 994 4856 4371
Fragments arranged by size:
4856 4371 994 758 6
BsaAI yAC'GTr
Cuts at: 2070 8338 2070
Size: 6268 4717
BsaBI GATnn'nnATC
Cuts at: 6 695 6461 7455 7629 8499 10536 6
Size: 689 5766 994 174 870 2037 455
Fragments arranged by size:
5766 2037 994 870 689 455 174
BsaHI Gr'CG_yC
Cuts at: 161 2851 8416 8664 9366 10862 161
Size: 2690 5565 248 702 1496 284
Fragments arranged by size:
5565 2690 1496 702 284 248
BsaWI w'CCGG_w
Cuts at: 3469 8174 8695 9373 9784 9931 10205 3469
Size: 4705 521 678 411 147 274 4249
Fragments arranged by size:
4705 4249 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4699 4729 6077 6107 6240 6270 6812 6842 4699
Size: 30 1348 30 133 30 542 30 8842
Fragments arranged by size:
8842 1348 542 133 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2342 2956 3121 3277 3439 3604 3766 4093 4425
Size: 614 165 156 162 165 162 327 332
Cuts at: 4425 4648 4720 2342
Size: 223 72 8607
Fragments arranged by size:
8607 614 332 327 223 165 165 162
162 156 72
BseYI C'CCAG_C
Cuts at: 188 1984 5285 6987 8410 9168 9833 10241 188
Size: 1796 3301 1702 1423 758 665 408 932
Fragments arranged by size:
3301 1796 1702 1423 932 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1650 2461 6735 7007 1650
Size: 811 4274 272 5628
Fragments arranged by size:
5628 4274 811 272
BsiEI CG_ry'CG
Cuts at: 104 8527 8573 9803 104
Size: 8423 46 1230 1286
Fragments arranged by size:
8423 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 5184 6694 7313 7999 8777 8967 9827 5184
Size: 1510 619 686 778 190 860 6342
Fragments arranged by size:
6342 1510 860 778 686 619 190
BsmI GAATG_Cn'
Cuts at: 1171 1645 6348 1171
Size: 474 4703 5808
Fragments arranged by size:
5808 4703 474
BsmBI CGTCTCn'nnnn_
Cuts at: 3283 3283
Size: 10985
Bsp1286I G_dGCh'C
Cuts at: 123 172 257 269 296 2456 5184 6694 6749
Size: 49 85 12 27 2160 2728 1510 55
Cuts at: 6749 6800 7313 7896 7908 7999 8610 8703 8777
Size: 51 513 583 12 91 611 93 74
Cuts at: 8777 8967 9029 9827 123
Size: 190 62 798 1281
Fragments arranged by size:
2728 2160 1510 1281 798 611 583 513
190 93 91 85 74 62 55 51
49 27 12 12
BspHI T'CATG_A
Cuts at: 4278 6216 9417 4278
Size: 1938 3201 5846
Fragments arranged by size:
5846 3201 1938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1452 2242 8035 8249 8551 8932 1452
Size: 790 5793 214 302 381 3505
Fragments arranged by size:
5793 3505 790 381 302 214
BsrBI CCG'CTC
Cuts at: 50 289 4124 9277 9331 10815 10980 50
Size: 239 3835 5153 54 1484 165 55
Fragments arranged by size:
5153 3835 1484 239 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2825 4137 8897 2825
Size: 1312 4760 4913
Fragments arranged by size:
4913 4760 1312
BsrFI r'CCGG_y
Cuts at: 8983 9164 8983
Size: 181 10804
BsrGI T'GTAC_A
Cuts at: 2211 4654 6618 2211
Size: 2443 1964 6578
Fragments arranged by size:
6578 2443 1964
BssSI C'ACGA_G
Cuts at: 3665 9256 9964 3665
Size: 5591 708 4686
Fragments arranged by size:
5591 4686 708
BstAPI GCAn_nnn'nTGC
Cuts at: 8362 8362
Size: 10985
BstBI TT'CG_AA
Cuts at: 1817 9346 1817
Size: 7529 3456
BstXI CCAn_nnnn'nTGG
Cuts at: 879 1056 2799 879
Size: 177 1743 9065
Fragments arranged by size:
9065 1743 177
BstYI r'GATC_y
Cuts at: 1 364 1396 3537 3988 3998 5833 6087 7194
Size: 363 1032 2141 451 10 1835 254 1107
Cuts at: 7194 7450 7456 7738 8010 8477 8500 8835 9081
Size: 256 6 282 272 467 23 335 246
Cuts at: 9081 9387 9399 9485 10537 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2141 1835 1107 1052 1032 467 451 449
363 335 306 282 272 256 254 246
86 23 12 10 6
BstZ17I GTA'TAC
Cuts at: 6854 6854
Size: 10985
Bsu36I CC'TnA_GG
Cuts at: 7383 7499 7383
Size: 116 10869
BtgI C'CryG_G
Cuts at: 227 248 1012 1303 7139 227
Size: 21 764 291 5836 4073
Fragments arranged by size:
5836 4073 764 291 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 8935 9116 9279 8935
Size: 181 163 10641
Fragments arranged by size:
10641 181 163
BtsI GCAGTG_nn'
Cuts at: 4182 7019 8228 4182
Size: 2837 1209 6939
Fragments arranged by size:
6939 2837 1209
DraI TTT'AAA
Cuts at: 1131 1280 2729 7984 1131
Size: 149 1449 5255 4132
Fragments arranged by size:
5255 4132 1449 149
DrdI GACnn_nn'nnGTC
Cuts at: 8691 10035 8691
Size: 1344 9641
EaeI y'GGCC_r
Cuts at: 8570 8744 9135 9162 10507 8570
Size: 174 391 27 1345 9048
Fragments arranged by size:
9048 1345 391 174 27
EagI C'GGCC_G
Cuts at: 8570 8570
Size: 10985
EarI CTCTTCn'nnn_
Cuts at: 809 1133 1235 1421 2384 2559 2594 2739 2820
Size: 324 102 186 963 175 35 145 81
Cuts at: 2820 2904 3150 3306 3552 3633 3989 4367 4453
Size: 84 246 156 246 81 356 378 86
Cuts at: 4453 5172 5462 5565 5714 5870 6680 6788 7155
Size: 719 290 103 149 156 810 108 367
Cuts at: 7155 7204 9008 9218 809
Size: 49 1804 210 2576
Fragments arranged by size:
2576 1804 963 810 719 378 367 356
324 290 246 246 210 186 175 156
156 149 145 108 103 102 86 84
81 81 49 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9193 9935 10081 10795 9193
Size: 742 146 714 9383
Fragments arranged by size:
9383 742 714 146
EcoICRI GAG'CTC
Cuts at: 6692 7997 6692
Size: 1305 9680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 734 815 966 1204 1256 1769 1784 2036
Size: 306 81 151 238 52 513 15 252
Cuts at: 2036 4013 4122 4233 4301 4819 5347 5441 5792
Size: 1977 109 111 68 518 528 94 351
Cuts at: 5792 5981 6632 6707 7106 7388 8810 9242 9595
Size: 189 651 75 399 282 1422 432 353
Cuts at: 9595 10257 428
Size: 662 1156
Fragments arranged by size:
1977 1422 1156 662 651 528 518 513
432 399 353 351 306 282 252 238
189 151 111 109 94 81 75 68
52 15
EcoNI CCTnn'n_nnAGG
Cuts at: 155 1443 1580 3424 155
Size: 1288 137 1844 7716
Fragments arranged by size:
7716 1844 1288 137
EcoO109I rG'GnC_Cy
Cuts at: 922 1573 6745 6746 7905 8124 922
Size: 651 5172 1 1159 219 3783
Fragments arranged by size:
5172 3783 1159 651 219 1
EcoRI G'AATT_C
Cuts at: 2034 7989 2034
Size: 5955 5030
EcoRV GAT'ATC
Cuts at: 4598 7953 4598
Size: 3355 7630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4469 4974 5329 9004 4469
Size: 505 355 3675 6450
Fragments arranged by size:
6450 3675 505 355
FspI TGC'GCA
Cuts at: 8766 8766
Size: 10985
HaeII r_GCGC'y
Cuts at: 164 1188 8419 8667 9897 10668 164
Size: 1024 7231 248 1230 771 481
Fragments arranged by size:
7231 1230 1024 771 481 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1276 1308 1750 1782 2115 2147 3895 3927 3973
Size: 32 442 32 333 32 1748 32 46
Cuts at: 3973 4005 4299 4331 4486 4518 4699 4731 5737
Size: 32 294 32 155 32 181 32 1006
Cuts at: 5737 5769 7186 7218 8827 8857 8859 8889 1276
Size: 32 1417 32 1609 30 2 30 3372
Fragments arranged by size:
3372 1748 1609 1417 1006 442 333 294
181 155 46 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1887 2191 5076 8024 1887
Size: 304 2885 2948 4848
Fragments arranged by size:
4848 2948 2885 304
HindIII A'AGCT_T
Cuts at: 4583 4583
Size: 10985
HpaI GTT'AAC
Cuts at: 5076 5076
Size: 10985
KasI G'GCGC_C
Cuts at: 160 8415 8663 160
Size: 8255 248 2482
Fragments arranged by size:
8255 2482 248
KpnI G_GTAC'C
Cuts at: 2692 6041 8005 2692
Size: 3349 1964 5672
Fragments arranged by size:
5672 3349 1964
MluI A'CGCG_T
Cuts at: 10418 10418
Size: 10985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1803 5303 5385 5403 6903 9744 9928 1803
Size: 3500 82 18 1500 2841 184 2860
Fragments arranged by size:
3500 2860 2841 1500 184 82 18
MscI TGG'CCA
Cuts at: 8746 8746
Size: 10985
MslI CAynn'nnrTG
Cuts at: 262 1054 2797 3895 4628 5701 5950 7306 7963
Size: 792 1743 1098 733 1073 249 1356 657
Cuts at: 7963 8974 9101 262
Size: 1011 127 2146
Fragments arranged by size:
2146 1743 1356 1098 1073 1011 792 733
657 249 127
MspA1I CmG'CkG
Cuts at: 250 8096 8410 8770 9554 9799 250
Size: 7846 314 360 784 245 1436
Fragments arranged by size:
7846 1436 784 360 314 245
NaeI GCC'GGC
Cuts at: 9166 9166
Size: 10985
NarI GG'CG_CC
Cuts at: 161 8416 8664 161
Size: 8255 248 2482
Fragments arranged by size:
8255 2482 248
NcoI C'CATG_G
Cuts at: 1012 7139 1012
Size: 6127 4858
NgoMIV G'CCGG_C
Cuts at: 9164 9164
Size: 10985
NheI G'CTAG_C
Cuts at: 3828 3828
Size: 10985
NsiI A_TGCA'T
Cuts at: 4199 5917 7470 7474 4199
Size: 1718 1553 4 7710
Fragments arranged by size:
7710 1718 1553 4
NspI r_CATG'y
Cuts at: 6617 6711 7472 8038 8538 10141 6617
Size: 94 761 566 500 1603 7461
Fragments arranged by size:
7461 1603 761 566 500 94
PasI CC'CwG_GG
Cuts at: 2759 7620 2759
Size: 4861 6124
PciI A'CATG_T
Cuts at: 6707 10137 6707
Size: 3430 7555
PfoI T'CCnGG_A
Cuts at: 1175 1175
Size: 10985
PmeI GTTT'AAAC
Cuts at: 7984 7984
Size: 10985
PmlI CAC'GTG
Cuts at: 2070 2070
Size: 10985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9431 9463 9431
Size: 32 10953
PpuMI rG'GwC_Cy
Cuts at: 1573 1573
Size: 10985
PshAI GACnn'nnGTC
Cuts at: 201 751 201
Size: 550 10435
PspOMI G'GGCC_C
Cuts at: 6745 7904 6745
Size: 1159 9826
PspXI vC'TCGA_Gb
Cuts at: 845 845
Size: 10985
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5921 5953 5921
Size: 32 10953
PstI C_TGCA'G
Cuts at: 2180 5267 8032 8717 2180
Size: 3087 2765 685 4448
Fragments arranged by size:
4448 3087 2765 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10985
PvuII CAG'CTG
Cuts at: 8410 8770 8410
Size: 360 10625
RsrII CG'GwC_CG
Cuts at: 9180 9180
Size: 10985
SacI G_AGCT'C
Cuts at: 6694 7999 6694
Size: 1305 9680
SacII CC_GC'GG
Cuts at: 251 251
Size: 10985
SalI G'TCGA_C
Cuts at: 8022 8022
Size: 10985
SapI GCTCTTCn'nnn_
Cuts at: 2739 5172 5462 5870 6788 9008 9218 2739
Size: 2433 290 408 918 2220 210 4506
Fragments arranged by size:
4506 2433 2220 918 408 290 210
SbfI CC_TGCA'GG
Cuts at: 8032 8032
Size: 10985
ScaI AGT'ACT
Cuts at: 321 408 783 4184 10502 321
Size: 87 375 3401 6318 804
Fragments arranged by size:
6318 3401 804 375 87
SexAI A'CCwGG_T
Cuts at: 6033 6033
Size: 10985
SfcI C'TryA_G
Cuts at: 33 899 2117 2176 3119 3602 3764 4205 4469
Size: 866 1218 59 943 483 162 441 264
Cuts at: 4469 5263 5435 8028 8713 9681 9872 33
Size: 794 172 2593 685 968 191 1146
Fragments arranged by size:
2593 1218 1146 968 943 866 794 685
483 441 264 191 172 162 59
SfoI GGC'GCC
Cuts at: 162 8417 8665 162
Size: 8255 248 2482
Fragments arranged by size:
8255 2482 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10985
SmaI CCC'GGG
Cuts at: 8007 8007
Size: 10985
SmlI C'TyrA_G
Cuts at: 845 3651 4092 4367 6427 6902 6938 7910 8140
Size: 2806 441 275 2060 475 36 972 230
Cuts at: 8140 9055 9769 10031 845
Size: 915 714 262 1799
Fragments arranged by size:
2806 2060 1799 972 915 714 475 441
275 262 230 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10985
SphI G_CATG'C
Cuts at: 7472 8038 8538 7472
Size: 566 500 9919
Fragments arranged by size:
9919 566 500
SspI AAT'ATT
Cuts at: 7830 7939 7830
Size: 109 10876
StuI AGG'CCT
Cuts at: 150 1620 150
Size: 1470 9515
StyI C'CwwG_G
Cuts at: 310 1012 2456 3004 3088 3169 3325 3487 4060
Size: 702 1444 548 84 81 156 162 573
Cuts at: 4060 5690 7139 8119 8473 310
Size: 1630 1449 980 354 2822
Fragments arranged by size:
2822 1630 1449 1444 980 702 573 548
354 162 156 84 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2149 4857 5299 8568 8701 9160 9369 2149
Size: 2708 442 3269 133 459 209 3765
Fragments arranged by size:
3765 3269 2708 459 442 209 133
TatI w'GTAC_w
Cuts at: 319 406 781 2211 4182 4654 6618 10500 319
Size: 87 375 1430 1971 472 1964 3882 804
Fragments arranged by size:
3882 1971 1964 1430 804 472 375 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6812 6844 10502 10534 6812
Size: 32 3658 32 7263
Fragments arranged by size:
7263 3658 32 32
Tth111I GACn'n_nGTC
Cuts at: 8782 8782
Size: 10985
XbaI T'CTAG_A
Cuts at: 1375 2772 7453 8016 1375
Size: 1397 4681 563 4344
Fragments arranged by size:
4681 4344 1397 563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5697 8480 5697
Size: 2783 8202
XhoI C'TCGA_G
Cuts at: 845 845
Size: 10985
XmaI C'CCGG_G
Cuts at: 8005 8005
Size: 10985
XmnI GAAnn'nnTTC
Cuts at: 5066 5066
Size: 10985
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI BaeI BglI BmgBI BsiWI BspEI BssHII BstEII
ClaI CspCI DraIII FseI FspAI MfeI NdeI NotI
NruI PacI PflMI PsiI SanDI SfiI SgrAI SnaBI
SrfI SwaI ZraI