Restriction map for
pF1KA1677
(Circular) (Six-base) MAPSORT of: pF1KA1677.seq Check: 4283 from: 1 to: 4925
pF1KA1677 4925 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1649 1963 1649
Size: 314 4611
Acc65I G'GTAC_C
Cuts at: 1941 1941
Size: 4925
AclI AA'CG_TT
Cuts at: 1919 4765 1919
Size: 2846 2079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 406 2750 3182 3535 4197 258
Size: 148 2344 432 353 662 986
Fragments arranged by size:
2344 986 662 432 353 148
AflIII A'CryG_T
Cuts at: 2277 4077 4358 2277
Size: 1800 281 2844
Fragments arranged by size:
2844 1800 281
AgeI A'CCGG_T
Cuts at: 346 1399 346
Size: 1053 3872
AhdI GACnn_n'nnGTC
Cuts at: 621 621
Size: 4925
AlwNI CAG_nnn'CTG
Cuts at: 1663 2230 3668 1663
Size: 567 1438 2920
Fragments arranged by size:
2920 1438 567
ApaI G_GGCC'C
Cuts at: 1123 1123
Size: 4925
ApaLI G'TGCA_C
Cuts at: 3763 3763
Size: 4925
ApoI r'AATT_y
Cuts at: 320 826 1132 1219 1327 1929 4278 4534 320
Size: 506 306 87 108 602 2349 256 711
Fragments arranged by size:
2349 711 602 506 306 256 108 87
AseI AT'TA_AT
Cuts at: 20 20
Size: 4925
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
AvaI C'yCGr_G
Cuts at: 1167 1945 1167
Size: 778 4147
BamHI G'GATC_C
Cuts at: 1335 1950 1335
Size: 615 4310
BanI G'GyrC_C
Cuts at: 1941 2355 2603 2638 1941
Size: 414 248 35 4228
Fragments arranged by size:
4228 414 248 35
BanII G_rGCy'C
Cuts at: 1123 1939 2969 1123
Size: 816 1030 3079
Fragments arranged by size:
3079 1030 816
BbeI G_GCGC'C
Cuts at: 2359 2607 2359
Size: 248 4677
BbsI GAAGACnn'nnnn_
Cuts at: 741 1575 741
Size: 834 4091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1049 1083 1049
Size: 34 4891
BciVI GTATCCnnnnn_n'
Cuts at: 2823 3879 4925 2823
Size: 1056 1046 2823
Fragments arranged by size:
2823 1056 1046
BclI T'GATC_A
Cuts at: 666 1665 2445 666
Size: 999 780 3146
Fragments arranged by size:
3146 999 780
BglII A'GATC_T
Cuts at: 488 2440 4477 488
Size: 1952 2037 936
Fragments arranged by size:
2037 1952 936
BlpI GC'TnA_GC
Cuts at: 2037 2037
Size: 4925
Bme1580I G_kGCm'C
Cuts at: 1123 2550 2643 3767 1123
Size: 1427 93 1124 2281
Fragments arranged by size:
2281 1427 1124 93
BmgBI CAC'GTC
Cuts at: 120 120
Size: 4925
BmrI ACTGGGnnnn_n'
Cuts at: 2137 2218 2553 4697 2137
Size: 81 335 2144 2365
Fragments arranged by size:
2365 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 4925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 762 762
Size: 4925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 1626 2101 2980 3694 3992 1475
Size: 151 475 879 714 298 2408
Fragments arranged by size:
2408 879 714 475 298 151
BsaI GGTCTCn'nnnn_
Cuts at: 4629 4629
Size: 4925
BsaAI yAC'GTr
Cuts at: 2278 2278
Size: 4925
BsaBI GATnn'nnATC
Cuts at: 6 665 1340 2439 4476 6
Size: 659 675 1099 2037 455
Fragments arranged by size:
2037 1099 675 659 455
BsaHI Gr'CG_yC
Cuts at: 2356 2604 3306 4802 2356
Size: 248 702 1496 2479
Fragments arranged by size:
2479 1496 702 248
BsaWI w'CCGG_w
Cuts at: 346 773 1399 1800 1884 2114 2635 3313 3724
Size: 427 626 401 84 230 521 678 411
Cuts at: 3724 3871 4145 346
Size: 147 274 1126
Fragments arranged by size:
1126 678 626 521 427 411 401 274
230 147 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 199
Size: 4925
BseYI C'CCAG_C
Cuts at: 163 1116 1450 2350 3108 3773 4181 163
Size: 953 334 900 758 665 408 907
Fragments arranged by size:
953 907 900 758 665 408 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 730 527
Size: 203 4722
BsiEI CG_ry'CG
Cuts at: 104 2467 2513 3743 104
Size: 2363 46 1230 1286
Fragments arranged by size:
2363 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 177 1379 1939 2717 2907 3767 177
Size: 1202 560 778 190 860 1335
Fragments arranged by size:
1335 1202 860 778 560 190
Bsp1286I G_dGCh'C
Cuts at: 177 1123 1379 1939 2550 2643 2717 2907 2969
Size: 946 256 560 611 93 74 190 62
Cuts at: 2969 3767 177
Size: 798 1335
Fragments arranged by size:
1335 946 798 611 560 256 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1800 1884 1800
Size: 84 4841
BspHI T'CATG_A
Cuts at: 411 3357 411
Size: 2946 1979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 668 1883 1975 2189 2491 2872 668
Size: 1215 92 214 302 381 2721
Fragments arranged by size:
2721 1215 381 302 214 92
BsrBI CCG'CTC
Cuts at: 50 3217 3271 4755 4920 50
Size: 3167 54 1484 165 55
Fragments arranged by size:
3167 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2837 2837
Size: 4925
BsrFI r'CCGG_y
Cuts at: 346 1399 2923 3104 346
Size: 1053 1524 181 2167
Fragments arranged by size:
2167 1524 1053 181
BssSI C'ACGA_G
Cuts at: 3196 3904 3196
Size: 708 4217
BstAPI GCAn_nnn'nTGC
Cuts at: 2302 2302
Size: 4925
BstBI TT'CG_AA
Cuts at: 1619 3286 1619
Size: 1667 3258
BstEII G'GTnAC_C
Cuts at: 1497 1497
Size: 4925
BstXI CCAn_nnnn'nTGG
Cuts at: 1025 1025
Size: 4925
BstYI r'GATC_y
Cuts at: 1 488 1335 1950 2417 2440 2775 3021 3327
Size: 487 847 615 467 23 335 246 306
Cuts at: 3327 3339 3425 4477 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 847 615 487 467 449 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1650 1650
Size: 4925
BtgI C'CryG_G
Cuts at: 107 1710 107
Size: 1603 3322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2875 3056 3219 2875
Size: 181 163 4581
Fragments arranged by size:
4581 181 163
BtsI GCAGTG_nn'
Cuts at: 2168 2168
Size: 4925
DraI TTT'AAA
Cuts at: 329 1924 329
Size: 1595 3330
DrdI GACnn_nn'nnGTC
Cuts at: 154 2631 3975 154
Size: 2477 1344 1104
Fragments arranged by size:
2477 1344 1104
EaeI y'GGCC_r
Cuts at: 254 1707 2510 2684 3075 3102 4447 254
Size: 1453 803 174 391 27 1345 732
Fragments arranged by size:
1453 1345 803 732 391 174 27
EagI C'GGCC_G
Cuts at: 2510 2510
Size: 4925
EarI CTCTTCn'nnn_
Cuts at: 1106 2948 3158 1106
Size: 1842 210 2873
Fragments arranged by size:
2873 1842 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3133 3875 4021 4735 3133
Size: 742 146 714 3323
Fragments arranged by size:
3323 742 714 146
EcoICRI GAG'CTC
Cuts at: 1937 1937
Size: 4925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 406 762 2750 3182 3535 4197 258
Size: 148 356 1988 432 353 662 986
Fragments arranged by size:
1988 986 662 432 356 353 148
EcoO109I rG'GnC_Cy
Cuts at: 1610 2064 1610
Size: 454 4471
EcoRI G'AATT_C
Cuts at: 1132 1929 1132
Size: 797 4128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4925
FspI TGC'GCA
Cuts at: 2706 2706
Size: 4925
HaeII r_GCGC'y
Cuts at: 2359 2607 3837 4608 2359
Size: 248 1230 771 2676
Fragments arranged by size:
2676 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 607 639 904 936 1292 1324 2767 2797 2799
Size: 32 265 32 356 32 1443 30 2
Cuts at: 2799 2829 607
Size: 30 2703
Fragments arranged by size:
2703 1443 356 265 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 864 990 1350 1964 864
Size: 126 360 614 3825
Fragments arranged by size:
3825 614 360 126
HindIII A'AGCT_T
Cuts at: 1573 1573
Size: 4925
HpaI GTT'AAC
Cuts at: 990 1350 990
Size: 360 4565
KasI G'GCGC_C
Cuts at: 2355 2603 2355
Size: 248 4677
KpnI G_GTAC'C
Cuts at: 1945 1945
Size: 4925
MluI A'CGCG_T
Cuts at: 4358 4358
Size: 4925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 890 3684 3868 890
Size: 2794 184 1947
Fragments arranged by size:
2794 1947 184
MscI TGG'CCA
Cuts at: 256 1709 2686 256
Size: 1453 977 2495
Fragments arranged by size:
2495 1453 977
MslI CAynn'nnrTG
Cuts at: 2914 3041 2914
Size: 127 4798
MspA1I CmG'CkG
Cuts at: 2036 2350 2710 3494 3739 2036
Size: 314 360 784 245 3222
Fragments arranged by size:
3222 784 360 314 245
NaeI GCC'GGC
Cuts at: 3106 3106
Size: 4925
NarI GG'CG_CC
Cuts at: 2356 2604 2356
Size: 248 4677
NcoI C'CATG_G
Cuts at: 107 1710 107
Size: 1603 3322
NgoMIV G'CCGG_C
Cuts at: 3104 3104
Size: 4925
NspI r_CATG'y
Cuts at: 1978 2478 4081 1978
Size: 500 1603 2822
Fragments arranged by size:
2822 1603 500
PciI A'CATG_T
Cuts at: 4077 4077
Size: 4925
PflMI CCAn_nnn'nTGG
Cuts at: 1508 1508
Size: 4925
PmeI GTTT'AAAC
Cuts at: 1924 1924
Size: 4925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4893
PsiI TTA'TAA
Cuts at: 1763 1763
Size: 4925
PspOMI G'GGCC_C
Cuts at: 1119 1119
Size: 4925
PstI C_TGCA'G
Cuts at: 294 1190 1972 2657 294
Size: 896 782 685 2562
Fragments arranged by size:
2562 896 782 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4925
PvuII CAG'CTG
Cuts at: 2350 2710 2350
Size: 360 4565
RsrII CG'GwC_CG
Cuts at: 3120 3120
Size: 4925
SacI G_AGCT'C
Cuts at: 1939 1939
Size: 4925
SalI G'TCGA_C
Cuts at: 1962 1962
Size: 4925
SapI GCTCTTCn'nnn_
Cuts at: 1106 2948 3158 1106
Size: 1842 210 2873
Fragments arranged by size:
2873 1842 210
SbfI CC_TGCA'GG
Cuts at: 1972 1972
Size: 4925
ScaI AGT'ACT
Cuts at: 4442 4442
Size: 4925
SfcI C'TryA_G
Cuts at: 33 290 386 953 1186 1226 1640 1968 2653
Size: 257 96 567 233 40 414 328 685
Cuts at: 2653 3621 3812 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 567 414 328 257 233
191 96 40
SfoI GGC'GCC
Cuts at: 2357 2605 2357
Size: 248 4677
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
SgrAI Cr'CCGG_yG
Cuts at: 1399 1399
Size: 4925
SmaI CCC'GGG
Cuts at: 1947 1947
Size: 4925
SmlI C'TyrA_G
Cuts at: 1490 1605 2080 2995 3709 3971 1490
Size: 115 475 915 714 262 2444
Fragments arranged by size:
2444 915 714 475 262 115
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4925
SphI G_CATG'C
Cuts at: 1978 2478 1978
Size: 500 4425
StuI AGG'CCT
Cuts at: 612 1754 612
Size: 1142 3783
StyI C'CwwG_G
Cuts at: 107 1710 2059 2413 107
Size: 1603 349 354 2619
Fragments arranged by size:
2619 1603 354 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 2508 2641 3100 3309 274
Size: 2234 133 459 209 1890
Fragments arranged by size:
2234 1890 459 209 133
TatI w'GTAC_w
Cuts at: 374 1893 4440 374
Size: 1519 2547 859
Fragments arranged by size:
2547 1519 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1782 1814 4442 4474 1782
Size: 32 2628 32 2233
Fragments arranged by size:
2628 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 2722 2722
Size: 4925
XbaI T'CTAG_A
Cuts at: 1956 1956
Size: 4925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1025 2420 1025
Size: 1395 3530
XmaI C'CCGG_G
Cuts at: 1945 1945
Size: 4925
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI