Restriction map for
pF1KA1664
(Circular) (Six-base) MAPSORT of: pF1KA1664.seq Check: 9131 from: 1 to: 5861
pF1KA1664 5861 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1802 1965 1802
Size: 163 5698
AatII G_ACGT'C
Cuts at: 370 370
Size: 5861
AccI GT'mk_AC
Cuts at: 2899 2899
Size: 5861
Acc65I G'GTAC_C
Cuts at: 2877 2877
Size: 5861
AclI AA'CG_TT
Cuts at: 5701 5701
Size: 5861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1045 1060 1177 1556 2272 3686 4118 4471
Size: 330 15 117 379 716 1414 432 353
Cuts at: 4471 5133 715
Size: 662 1443
Fragments arranged by size:
1443 1414 716 662 432 379 353 330
117 15
AfeI AGC'GCT
Cuts at: 228 1102 228
Size: 874 4987
AflIII A'CryG_T
Cuts at: 773 2009 2138 2498 3213 5013 5294 773
Size: 1236 129 360 715 1800 281 1340
Fragments arranged by size:
1800 1340 1236 715 360 281 129
AgeI A'CCGG_T
Cuts at: 2240 2240
Size: 5861
AleI CACnn'nnGTG
Cuts at: 1879 1879
Size: 5861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 751 1830 751
Size: 1079 4782
AlwNI CAG_nnn'CTG
Cuts at: 388 1767 2445 2589 2637 2655 2699 3166 4604
Size: 1379 678 144 48 18 44 467 1438
Cuts at: 4604 388
Size: 1645
Fragments arranged by size:
1645 1438 1379 678 467 144 48 44
18
ApaI G_GGCC'C
Cuts at: 461 461
Size: 5861
ApaLI G'TGCA_C
Cuts at: 1297 1810 1882 4699 1297
Size: 513 72 2817 2459
Fragments arranged by size:
2817 2459 513 72
ApoI r'AATT_y
Cuts at: 2113 2865 5214 5470 2113
Size: 752 2349 256 2504
Fragments arranged by size:
2504 2349 752 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5861
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5861
AvaI C'yCGr_G
Cuts at: 491 523 1345 2881 491
Size: 32 822 1536 3471
Fragments arranged by size:
3471 1536 822 32
BamHI G'GATC_C
Cuts at: 1718 2763 2886 1718
Size: 1045 123 4693
Fragments arranged by size:
4693 1045 123
BanI G'GyrC_C
Cuts at: 213 1087 1594 2098 2243 2663 2877 3291 3539
Size: 874 507 504 145 420 214 414 248
Cuts at: 3539 3574 213
Size: 35 2500
Fragments arranged by size:
2500 874 507 504 420 414 248 214
145 35
BanII G_rGCy'C
Cuts at: 167 257 461 1573 1712 1748 2875 3905 167
Size: 90 204 1112 139 36 1127 1030 2123
Fragments arranged by size:
2123 1127 1112 1030 204 139 90 36
BbeI G_GCGC'C
Cuts at: 217 1091 2667 3295 3543 217
Size: 874 1576 628 248 2535
Fragments arranged by size:
2535 1576 874 628 248
BbsI GAAGACnn'nnnn_
Cuts at: 589 1484 1526 2434 589
Size: 895 42 908 4016
Fragments arranged by size:
4016 908 895 42
BbvCI CC'TCA_GC
Cuts at: 1054 1995 1054
Size: 941 4920
BciVI GTATCCnnnnn_n'
Cuts at: 3759 4815 5861 3759
Size: 1056 1046 3759
Fragments arranged by size:
3759 1056 1046
BclI T'GATC_A
Cuts at: 1208 1502 3381 1208
Size: 294 1879 3688
Fragments arranged by size:
3688 1879 294
BfrBI ATG'CAT
Cuts at: 2388 2388
Size: 5861
BglI GCCn_nnn'nGGC
Cuts at: 137 637 137
Size: 500 5361
BglII A'GATC_T
Cuts at: 3376 5413 3376
Size: 2037 3824
BlpI GC'TnA_GC
Cuts at: 1572 2973 1572
Size: 1401 4460
Bme1580I G_kGCm'C
Cuts at: 281 461 633 1272 1301 1368 1610 1814 1886
Size: 180 172 639 29 67 242 204 72
Cuts at: 1886 2845 3486 3579 4703 281
Size: 959 641 93 1124 1439
Fragments arranged by size:
1439 1124 959 641 639 242 204 180
172 93 72 67 29
BmgBI CAC'GTC
Cuts at: 236 236
Size: 5861
BmrI ACTGGGnnnn_n'
Cuts at: 2817 3073 3154 3489 5633 2817
Size: 256 81 335 2144 3045
Fragments arranged by size:
3045 2144 335 256 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 456 504 4228 456
Size: 48 3724 2089
Fragments arranged by size:
3724 2089 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 934 1081 1587 1698 1744 1801 2040 2049
Size: 325 147 506 111 46 57 239 9
Cuts at: 2049 2659 2695 609
Size: 610 36 3775
Fragments arranged by size:
3775 610 506 325 239 147 111 57
46 36 9
Bpu10I CC'TnA_GC
Cuts at: 441 1054 1995 441
Size: 613 941 4307
Fragments arranged by size:
4307 941 613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 853 1111 3037 3916 4630 4928 661
Size: 192 258 1926 879 714 298 1594
Fragments arranged by size:
1926 1594 879 714 298 258 192
BsaI GGTCTCn'nnnn_
Cuts at: 1915 5565 1915
Size: 3650 2211
BsaAI yAC'GTr
Cuts at: 776 1061 2010 2139 2340 3214 776
Size: 285 949 129 201 874 3423
Fragments arranged by size:
3423 949 874 285 201 129
BsaBI GATnn'nnATC
Cuts at: 6 2050 3375 5412 6
Size: 2044 1325 2037 455
Fragments arranged by size:
2044 2037 1325 455
BsaHI Gr'CG_yC
Cuts at: 214 367 1088 2664 3292 3540 4242 5738 214
Size: 153 721 1576 628 248 702 1496 337
Fragments arranged by size:
1576 1496 721 702 628 337 248 153
BsaWI w'CCGG_w
Cuts at: 285 2240 3050 3571 4249 4660 4807 5081 285
Size: 1955 810 521 678 411 147 274 1065
Fragments arranged by size:
1955 1065 810 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1822 1852 1999 2029 2485 2515 1822
Size: 30 147 30 456 30 5168
Fragments arranged by size:
5168 456 147 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 578 2557 2656 578
Size: 1979 99 3783
Fragments arranged by size:
3783 1979 99
BseYI C'CCAG_C
Cuts at: 263 269 388 454 723 993 1914 2398 2407
Size: 6 119 66 269 270 921 484 9
Cuts at: 2407 3286 4044 4709 5117 263
Size: 879 758 665 408 1007
Fragments arranged by size:
1007 921 879 758 665 484 408 270
269 119 66 9 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1800 997
Size: 803 5058
BsiEI CG_ry'CG
Cuts at: 104 153 424 1378 1704 3403 3449 4679 104
Size: 49 271 954 326 1699 46 1230 1286
Fragments arranged by size:
1699 1286 1230 954 326 271 49 46
BsiHKAI G_wGCw'C
Cuts at: 1301 1573 1712 1814 1886 2155 2377 2540 2875
Size: 272 139 102 72 269 222 163 335
Cuts at: 2875 3653 3843 4703 1301
Size: 778 190 860 2459
Fragments arranged by size:
2459 860 778 335 272 269 222 190
163 139 102 72
BsmI GAATG_Cn'
Cuts at: 1643 1643
Size: 5861
BsmBI CGTCTCn'nnnn_
Cuts at: 355 355
Size: 5861
Bsp1286I G_dGCh'C
Cuts at: 167 257 281 461 633 1272 1301 1368 1573
Size: 90 24 180 172 639 29 67 205
Cuts at: 1573 1610 1712 1748 1814 1886 2155 2377 2540
Size: 37 102 36 66 72 269 222 163
Cuts at: 2540 2845 2875 3486 3579 3653 3843 3905 4703
Size: 305 30 611 93 74 190 62 798
Cuts at: 4703 167
Size: 1325
Fragments arranged by size:
1325 798 639 611 305 269 222 205
190 180 172 163 102 93 90 74
72 67 66 62 37 36 30 29
24
BspHI T'CATG_A
Cuts at: 1685 4293 1685
Size: 2608 3253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1802 1965 2911 3125 3427 3808 1802
Size: 163 946 214 302 381 3855
Fragments arranged by size:
3855 946 381 302 214 163
BsrBI CCG'CTC
Cuts at: 50 155 291 486 1173 1350 4153 4207 5691
Size: 105 136 195 687 177 2803 54 1484
Cuts at: 5691 5856 50
Size: 165 55
Fragments arranged by size:
2803 1484 687 195 177 165 136 105
55 54
BsrDI GCAATG_nn'
Cuts at: 735 1071 3773 735
Size: 336 2702 2823
Fragments arranged by size:
2823 2702 336
BsrFI r'CCGG_y
Cuts at: 890 1373 1377 1728 2240 2666 3859 4040 890
Size: 483 4 351 512 426 1193 181 2711
Fragments arranged by size:
2711 1193 512 483 426 351 181 4
BsrGI T'GTAC_A
Cuts at: 2792 2792
Size: 5861
BssSI C'ACGA_G
Cuts at: 1603 2352 2539 4132 4840 1603
Size: 749 187 1593 708 2624
Fragments arranged by size:
2624 1593 749 708 187
BstAPI GCAn_nnn'nTGC
Cuts at: 1296 2699 3238 1296
Size: 1403 539 3919
Fragments arranged by size:
3919 1403 539
BstBI TT'CG_AA
Cuts at: 4222 4222
Size: 5861
BstXI CCAn_nnnn'nTGG
Cuts at: 1827 2125 1827
Size: 298 5563
BstYI r'GATC_y
Cuts at: 1 1130 1718 2763 2886 3353 3376 3711 3957
Size: 1129 588 1045 123 467 23 335 246
Cuts at: 3957 4263 4275 4361 5413 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1129 1052 1045 588 467 449 335 306
246 123 86 23 12
BtgI C'CryG_G
Cuts at: 107 159 780 1472 1559 107
Size: 52 621 692 87 4409
Fragments arranged by size:
4409 692 621 87 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1059 1875 3811 3992 4155 1059
Size: 816 1936 181 163 2765
Fragments arranged by size:
2765 1936 816 181 163
BtsI GCAGTG_nn'
Cuts at: 341 904 1270 1812 1814 3104 341
Size: 563 366 542 2 1290 3098
Fragments arranged by size:
3098 1290 563 542 366 2
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1541 1576 1541
Size: 35 5826
DraI TTT'AAA
Cuts at: 2860 2860
Size: 5861
DraIII CAC_nnn'GTG
Cuts at: 2010 2139 2010
Size: 129 5732
DrdI GACnn_nn'nnGTC
Cuts at: 3567 4911 3567
Size: 1344 4517
EaeI y'GGCC_r
Cuts at: 150 421 920 1371 1375 1379 1701 1847 3446
Size: 271 499 451 4 4 322 146 1599
Cuts at: 3446 3620 4011 4038 5383 150
Size: 174 391 27 1345 628
Fragments arranged by size:
1599 1345 628 499 451 391 322 271
174 146 27 4 4
EagI C'GGCC_G
Cuts at: 150 421 1375 1701 3446 150
Size: 271 954 326 1745 2565
Fragments arranged by size:
2565 1745 954 326 271
EarI CTCTTCn'nnn_
Cuts at: 566 1189 2143 2708 2749 3884 4094 566
Size: 623 954 565 41 1135 210 2333
Fragments arranged by size:
2333 1135 954 623 565 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2740 4069 4811 4957 5671 2740
Size: 1329 742 146 714 2930
Fragments arranged by size:
2930 1329 742 714 146
EcoICRI GAG'CTC
Cuts at: 1571 1710 2873 1571
Size: 139 1163 4559
Fragments arranged by size:
4559 1163 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 715 934 1045 1060 1081 1177 1556 1587
Size: 106 219 111 15 21 96 379 31
Cuts at: 1587 1698 1744 1801 2040 2049 2272 2659 2695
Size: 111 46 57 239 9 223 387 36
Cuts at: 2695 3686 4118 4471 5133 609
Size: 991 432 353 662 1337
Fragments arranged by size:
1337 991 662 432 387 379 353 239
223 219 111 111 106 96 57 46
36 31 21 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2038 2038
Size: 5861
EcoO109I rG'GnC_Cy
Cuts at: 458 1776 3000 458
Size: 1318 1224 3319
Fragments arranged by size:
3319 1318 1224
EcoRI G'AATT_C
Cuts at: 2113 2865 2113
Size: 752 5109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3880 3880
Size: 5861
FseI GG_CCGG'CC
Cuts at: 1377 1381 1377
Size: 4 5857
FspI TGC'GCA
Cuts at: 2003 3642 2003
Size: 1639 4222
HaeII r_GCGC'y
Cuts at: 217 230 711 1091 1104 2557 2655 2667 3295
Size: 13 481 380 13 1453 98 12 628
Cuts at: 3295 3543 4773 5544 217
Size: 248 1230 771 534
Fragments arranged by size:
1453 1230 771 628 534 481 380 248
98 13 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 517 549 675 707 936 968 1236
Size: 32 133 32 126 32 229 32 268
Cuts at: 1236 1268 1471 1503 1555 1582 1587 1614 1735
Size: 32 203 32 52 27 5 27 121
Cuts at: 1735 1767 2748 2780 3703 3733 3735 3765 352
Size: 32 981 32 923 30 2 30 2448
Fragments arranged by size:
2448 981 923 268 229 203 133 126
121 52 32 32 32 32 32 32
32 32 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 2900 2900
Size: 5861
KasI G'GCGC_C
Cuts at: 213 1087 2663 3291 3539 213
Size: 874 1576 628 248 2535
Fragments arranged by size:
2535 1576 874 628 248
KpnI G_GTAC'C
Cuts at: 2881 2881
Size: 5861
MluI A'CGCG_T
Cuts at: 5294 5294
Size: 5861
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 1234 4620 4804 296
Size: 938 3386 184 1353
Fragments arranged by size:
3386 1353 938 184
MscI TGG'CCA
Cuts at: 922 3622 922
Size: 2700 3161
MslI CAynn'nnrTG
Cuts at: 959 1498 1729 1879 2176 3850 3977 959
Size: 539 231 150 297 1674 127 2843
Fragments arranged by size:
2843 1674 539 297 231 150 127
MspA1I CmG'CkG
Cuts at: 161 300 713 1266 1323 1764 1999 2250 2478
Size: 139 413 553 57 441 235 251 228
Cuts at: 2478 2586 2634 2699 2769 2972 3286 3646 4430
Size: 108 48 65 70 203 314 360 784
Cuts at: 4430 4675 161
Size: 245 1347
Fragments arranged by size:
1347 784 553 441 413 360 314 251
245 235 228 203 139 108 70 65
57 48
NaeI GCC'GGC
Cuts at: 1375 1379 2668 4042 1375
Size: 4 1289 1374 3194
Fragments arranged by size:
3194 1374 1289 4
NarI GG'CG_CC
Cuts at: 214 1088 2664 3292 3540 214
Size: 874 1576 628 248 2535
Fragments arranged by size:
2535 1576 874 628 248
NcoI C'CATG_G
Cuts at: 107 1472 107
Size: 1365 4496
NdeI CA'TA_TG
Cuts at: 2837 2837
Size: 5861
NgoMIV G'CCGG_C
Cuts at: 1373 1377 2666 4040 1373
Size: 4 1289 1374 3194
Fragments arranged by size:
3194 1374 1289 4
NotI GC'GGCC_GC
Cuts at: 150 150
Size: 5861
NsiI A_TGCA'T
Cuts at: 2390 2390
Size: 5861
NspI r_CATG'y
Cuts at: 2502 2914 3414 5017 2502
Size: 412 500 1603 3346
Fragments arranged by size:
3346 1603 500 412
PciI A'CATG_T
Cuts at: 2498 5013 2498
Size: 2515 3346
PflMI CCAn_nnn'nTGG
Cuts at: 388 1797 388
Size: 1409 4452
PfoI T'CCnGG_A
Cuts at: 1714 1714
Size: 5861
PmeI GTTT'AAAC
Cuts at: 2860 2860
Size: 5861
PmlI CAC'GTG
Cuts at: 776 1061 776
Size: 285 5576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 4307
Size: 32 5829
PpuMI rG'GwC_Cy
Cuts at: 1776 1776
Size: 5861
PshAI GACnn'nnGTC
Cuts at: 300 525 300
Size: 225 5636
PspOMI G'GGCC_C
Cuts at: 457 457
Size: 5861
PspXI vC'TCGA_Gb
Cuts at: 1345 1345
Size: 5861
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2488 2520 2488
Size: 32 5829
PstI C_TGCA'G
Cuts at: 413 1222 1277 2704 2908 3593 413
Size: 809 55 1427 204 685 2681
Fragments arranged by size:
2681 1427 809 685 204 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5861
PvuII CAG'CTG
Cuts at: 1323 1999 2250 2699 3286 3646 1323
Size: 676 251 449 587 360 3538
Fragments arranged by size:
3538 676 587 449 360 251
RsrII CG'GwC_CG
Cuts at: 4056 4056
Size: 5861
SacI G_AGCT'C
Cuts at: 1573 1712 2875 1573
Size: 139 1163 4559
Fragments arranged by size:
4559 1163 139
SacII CC_GC'GG
Cuts at: 162 162
Size: 5861
SalI G'TCGA_C
Cuts at: 2898 2898
Size: 5861
SapI GCTCTTCn'nnn_
Cuts at: 2143 3884 4094 2143
Size: 1741 210 3910
Fragments arranged by size:
3910 1741 210
SbfI CC_TGCA'GG
Cuts at: 1222 2908 1222
Size: 1686 4175
ScaI AGT'ACT
Cuts at: 1462 5378 1462
Size: 3916 1945
SfcI C'TryA_G
Cuts at: 33 409 747 1218 1273 1662 2265 2700 2904
Size: 376 338 471 55 389 603 435 204
Cuts at: 2904 3589 4557 4748 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 603 471 435 389 376
338 204 191 55
SfoI GGC'GCC
Cuts at: 215 1089 2665 3293 3541 215
Size: 874 1576 628 248 2535
Fragments arranged by size:
2535 1576 874 628 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5861
SgrAI Cr'CCGG_yG
Cuts at: 2666 2666
Size: 5861
SmaI CCC'GGG
Cuts at: 493 2883 493
Size: 2390 3471
SmlI C'TyrA_G
Cuts at: 676 868 1126 1345 3016 3931 4645 4907 676
Size: 192 258 219 1671 915 714 262 1630
Fragments arranged by size:
1671 1630 915 714 262 258 219 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5861
SphI G_CATG'C
Cuts at: 2914 3414 2914
Size: 500 5361
StuI AGG'CCT
Cuts at: 2680 2680
Size: 5861
StyI C'CwwG_G
Cuts at: 107 923 929 1472 1514 1610 2378 2816 2995
Size: 816 6 543 42 96 768 438 179
Cuts at: 2995 3349 107
Size: 354 2619
Fragments arranged by size:
2619 816 768 543 438 354 179 96
42 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3444 3577 4036 4245 3444
Size: 133 459 209 5060
Fragments arranged by size:
5060 459 209 133
TatI w'GTAC_w
Cuts at: 1460 1584 2501 2792 5376 1460
Size: 124 917 291 2584 1945
Fragments arranged by size:
2584 1945 917 291 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1822 1854 5378 5410 1822
Size: 32 3524 32 2273
Fragments arranged by size:
3524 2273 32 32
Tth111I GACn'n_nGTC
Cuts at: 366 2445 3658 366
Size: 2079 1213 2569
Fragments arranged by size:
2569 2079 1213
XbaI T'CTAG_A
Cuts at: 2892 2892
Size: 5861
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 633 778 882 1320 1366 3356 633
Size: 145 104 438 46 1990 3138
Fragments arranged by size:
3138 1990 438 145 104 46
XhoI C'TCGA_G
Cuts at: 1345 1345
Size: 5861
XmaI C'CCGG_G
Cuts at: 491 2881 491
Size: 2390 3471
ZraI GAC'GTC
Cuts at: 368 368
Size: 5861
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BcgI BmtI
BsiWI BspEI BssHII BstEII BstZ17I Bsu36I ClaI EcoRV
FspAI HindIII HpaI MfeI NheI NruI PacI PasI
PsiI SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XmnI