Restriction map for
pF1KA1649
(Circular) (Six-base) MAPSORT of: pF1KA1649.seq Check: 9557 from: 1 to: 4376
pF1KA1649 4376 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1414 1414
Size: 4376
Acc65I G'GTAC_C
Cuts at: 1392 1392
Size: 4376
AclI AA'CG_TT
Cuts at: 4216 4216
Size: 4376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1324 2201 2633 3380 3648 556
Size: 768 877 432 747 268 1284
Fragments arranged by size:
1284 877 768 747 432 268
AflIII A'CryG_T
Cuts at: 863 1728 2832 3809 863
Size: 865 1104 977 1430
Fragments arranged by size:
1430 1104 977 865
AgeI A'CCGG_T
Cuts at: 1115 2764 1115
Size: 1649 2727
AleI CACnn'nnGTG
Cuts at: 793 793
Size: 4376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 368 368
Size: 4376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1243 1275 1243
Size: 32 4344
AlwNI CAG_nnn'CTG
Cuts at: 823 916 1681 3248 823
Size: 93 765 1567 1951
Fragments arranged by size:
1951 1567 765 93
ApaI G_GGCC'C
Cuts at: 1023 1023
Size: 4376
ApaLI G'TGCA_C
Cuts at: 3146 3146
Size: 4376
ApoI r'AATT_y
Cuts at: 645 1354 1380 3729 3985 645
Size: 709 26 2349 256 1036
Fragments arranged by size:
2349 1036 709 256 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4376
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
AvaI C'yCGr_G
Cuts at: 203 329 458 974 1396 203
Size: 126 129 516 422 3183
Fragments arranged by size:
3183 516 422 129 126
BamHI G'GATC_C
Cuts at: 212 1401 212
Size: 1189 3187
BanI G'GyrC_C
Cuts at: 426 579 943 1392 1806 2054 2089 426
Size: 153 364 449 414 248 35 2713
Fragments arranged by size:
2713 449 414 364 248 153 35
BanII G_rGCy'C
Cuts at: 1023 1035 1390 2420 1023
Size: 12 355 1030 2979
Fragments arranged by size:
2979 1030 355 12
BbeI G_GCGC'C
Cuts at: 1810 2058 1810
Size: 248 4128
BciVI GTATCCnnnnn_n'
Cuts at: 2274 3035 4376 2274
Size: 761 1341 2274
Fragments arranged by size:
2274 1341 761
BclI T'GATC_A
Cuts at: 1896 1896
Size: 4376
BfrBI ATG'CAT
Cuts at: 811 811
Size: 4376
BglII A'GATC_T
Cuts at: 1891 3928 1891
Size: 2037 2339
BlpI GC'TnA_GC
Cuts at: 748 917 1488 748
Size: 169 571 3636
Fragments arranged by size:
3636 571 169
Bme1580I G_kGCm'C
Cuts at: 431 1023 2001 2094 3150 431
Size: 592 978 93 1056 1657
Fragments arranged by size:
1657 1056 978 592 93
BmrI ACTGGGnnnn_n'
Cuts at: 322 1588 1669 2004 4148 322
Size: 1266 81 335 2144 550
Fragments arranged by size:
2144 1266 550 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 4376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1552 2431 2923 3221 1009
Size: 543 879 492 298 2164
Fragments arranged by size:
2164 879 543 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4080 4080
Size: 4376
BsaAI yAC'GTr
Cuts at: 1729 1729
Size: 4376
BsaBI GATnn'nnATC
Cuts at: 6 1890 3927 6
Size: 1884 2037 455
Fragments arranged by size:
2037 1884 455
BsaHI Gr'CG_yC
Cuts at: 1807 2055 2757 4253 1807
Size: 248 702 1496 1930
Fragments arranged by size:
1930 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1115 1565 2086 2764 3038 3185 3596 1115
Size: 450 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 450 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1051 1081 1243 1273 1051
Size: 30 162 30 4154
Fragments arranged by size:
4154 162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 495 1000 1251 1254 495
Size: 505 251 3 3617
Fragments arranged by size:
3617 505 251 3
BseYI C'CCAG_C
Cuts at: 742 1801 2559 3136 3632 742
Size: 1059 758 577 496 1486
Fragments arranged by size:
1486 1059 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 952 400
Size: 552 3824
BsiEI CG_ry'CG
Cuts at: 104 1918 1964 3172 104
Size: 1814 46 1208 1308
Fragments arranged by size:
1814 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1035 1390 2168 2358 3150 1035
Size: 355 778 190 792 2261
Fragments arranged by size:
2261 792 778 355 190
Bsp1286I G_dGCh'C
Cuts at: 431 1023 1035 1390 2001 2094 2168 2358 2420
Size: 592 12 355 611 93 74 190 62
Cuts at: 2420 3150 431
Size: 730 1657
Fragments arranged by size:
1657 730 611 592 355 190 93 74
62 12
BspEI T'CCGG_A
Cuts at: 3596 3596
Size: 4376
BspHI T'CATG_A
Cuts at: 3552 3552
Size: 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1426 1640 1942 2323 1426
Size: 214 302 381 3479
Fragments arranged by size:
3479 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2668 2722 4206 4371 50
Size: 2618 54 1484 165 55
Fragments arranged by size:
2618 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2288 2288
Size: 4376
BsrFI r'CCGG_y
Cuts at: 1115 2374 2555 2764 1115
Size: 1259 181 209 2727
Fragments arranged by size:
2727 1259 209 181
BssSI C'ACGA_G
Cuts at: 2647 3005 2647
Size: 358 4018
BstAPI GCAn_nnn'nTGC
Cuts at: 1753 1753
Size: 4376
BstBI TT'CG_AA
Cuts at: 363 843 2737 363
Size: 480 1894 2002
Fragments arranged by size:
2002 1894 480
BstXI CCAn_nnnn'nTGG
Cuts at: 742 742
Size: 4376
BstYI r'GATC_y
Cuts at: 1 212 1401 1868 1891 2226 2472 3484 3570
Size: 211 1189 467 23 335 246 1012 86
Cuts at: 3570 3582 3928 1
Size: 12 346 449
Fragments arranged by size:
1189 1012 467 449 346 335 246 211
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 717 1323 717
Size: 606 3770
BtgI C'CryG_G
Cuts at: 107 422 575 107
Size: 315 153 3908
Fragments arranged by size:
3908 315 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2326 2507 2670 2326
Size: 181 163 4032
Fragments arranged by size:
4032 181 163
BtsI GCAGTG_nn'
Cuts at: 1619 1619
Size: 4376
DraI TTT'AAA
Cuts at: 1375 1375
Size: 4376
DrdI GACnn_nn'nnGTC
Cuts at: 864 2082 2940 864
Size: 1218 858 2300
Fragments arranged by size:
2300 1218 858
EaeI y'GGCC_r
Cuts at: 1961 2135 2526 2553 3898 1961
Size: 174 391 27 1345 2439
Fragments arranged by size:
2439 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1961 1961
Size: 4376
EarI CTCTTCn'nnn_
Cuts at: 472 1318 2399 2609 472
Size: 846 1081 210 2239
Fragments arranged by size:
2239 1081 846 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2584 2894 3040 4186 127
Size: 2457 310 146 1146 317
Fragments arranged by size:
2457 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1033 1388 1033
Size: 355 4021
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1324 2201 2633 3380 3648 556
Size: 768 877 432 747 268 1284
Fragments arranged by size:
1284 877 768 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 777 1019 1020 1328 1515 777
Size: 242 1 308 187 3638
Fragments arranged by size:
3638 308 242 187 1
EcoRI G'AATT_C
Cuts at: 1354 1380 1354
Size: 26 4350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 184 489 1329 2395 184
Size: 305 840 1066 2165
Fragments arranged by size:
2165 1066 840 305
FspI TGC'GCA
Cuts at: 2157 2157
Size: 4376
HaeII r_GCGC'y
Cuts at: 1810 2058 3080 4059 1810
Size: 248 1022 979 2127
Fragments arranged by size:
2127 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 197 229 350 382 516 548 1198 1230 2218
Size: 32 121 32 134 32 650 32 988
Cuts at: 2218 2248 2250 2280 197
Size: 30 2 30 2293
Fragments arranged by size:
2293 988 650 134 121 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 239 1415 239
Size: 1176 3200
HindIII A'AGCT_T
Cuts at: 337 760 1366 337
Size: 423 606 3347
Fragments arranged by size:
3347 606 423
KasI G'GCGC_C
Cuts at: 1806 2054 1806
Size: 248 4128
KpnI G_GTAC'C
Cuts at: 1396 1396
Size: 4376
MluI A'CGCG_T
Cuts at: 3809 3809
Size: 4376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 168 1292 3047 3231 168
Size: 1124 1755 184 1313
Fragments arranged by size:
1755 1313 1124 184
MscI TGG'CCA
Cuts at: 2137 2137
Size: 4376
MslI CAynn'nnrTG
Cuts at: 793 949 1157 2365 2492 793
Size: 156 208 1208 127 2677
Fragments arranged by size:
2677 1208 208 156 127
MspA1I CmG'CkG
Cuts at: 913 1487 1801 2161 3174 3419 913
Size: 574 314 360 1013 245 1870
Fragments arranged by size:
1870 1013 574 360 314 245
NaeI GCC'GGC
Cuts at: 2557 2557
Size: 4376
NarI GG'CG_CC
Cuts at: 1807 2055 1807
Size: 248 4128
NcoI C'CATG_G
Cuts at: 107 575 107
Size: 468 3908
NgoMIV G'CCGG_C
Cuts at: 2555 2555
Size: 4376
NsiI A_TGCA'T
Cuts at: 813 813
Size: 4376
NspI r_CATG'y
Cuts at: 867 1429 1929 2836 867
Size: 562 500 907 2407
Fragments arranged by size:
2407 907 562 500
PciI A'CATG_T
Cuts at: 863 2832 863
Size: 1969 2407
PflMI CCAn_nnn'nTGG
Cuts at: 514 738 514
Size: 224 4152
PfoI T'CCnGG_A
Cuts at: 868 868
Size: 4376
PmeI GTTT'AAAC
Cuts at: 1375 1375
Size: 4376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1243 1275 3515 3547 1243
Size: 32 2240 32 2072
Fragments arranged by size:
2240 2072 32 32
PspOMI G'GGCC_C
Cuts at: 1019 1019
Size: 4376
PspXI vC'TCGA_Gb
Cuts at: 974 974
Size: 4376
PstI C_TGCA'G
Cuts at: 1423 2108 1423
Size: 685 3691
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4376
PvuII CAG'CTG
Cuts at: 913 1801 2161 913
Size: 888 360 3128
Fragments arranged by size:
3128 888 360
RsrII CG'GwC_CG
Cuts at: 2571 2571
Size: 4376
SacI G_AGCT'C
Cuts at: 1035 1390 1035
Size: 355 4021
SalI G'TCGA_C
Cuts at: 1413 1413
Size: 4376
SapI GCTCTTCn'nnn_
Cuts at: 2399 2609 2399
Size: 210 4166
SbfI CC_TGCA'GG
Cuts at: 1423 1423
Size: 4376
ScaI AGT'ACT
Cuts at: 3893 3893
Size: 4376
SfcI C'TryA_G
Cuts at: 33 1419 2104 3097 3288 33
Size: 1386 685 993 191 1121
Fragments arranged by size:
1386 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1808 2056 1808
Size: 248 4128
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
SmaI CCC'GGG
Cuts at: 331 460 1398 331
Size: 129 938 3309
Fragments arranged by size:
3309 938 129
SmlI C'TyrA_G
Cuts at: 203 974 1024 1531 2446 2938 3200 203
Size: 771 50 507 915 492 262 1379
Fragments arranged by size:
1379 915 771 507 492 262 50
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4376
SphI G_CATG'C
Cuts at: 1429 1929 1429
Size: 500 3876
StuI AGG'CCT
Cuts at: 224 224
Size: 4376
StyI C'CwwG_G
Cuts at: 107 575 773 1510 1864 107
Size: 468 198 737 354 2619
Fragments arranged by size:
2619 737 468 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 525 1959 2092 2551 2760 525
Size: 1434 133 459 209 2141
Fragments arranged by size:
2141 1434 459 209 133
TatI w'GTAC_w
Cuts at: 1106 3891 1106
Size: 2785 1591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3893 3925 3893
Size: 32 4344
Tth111I GACn'n_nGTC
Cuts at: 2173 2173
Size: 4376
XbaI T'CTAG_A
Cuts at: 1407 1407
Size: 4376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 739 1871 739
Size: 1132 3244
XhoI C'TCGA_G
Cuts at: 203 974 203
Size: 771 3605
XmaI C'CCGG_G
Cuts at: 329 458 1396 329
Size: 129 938 3309
Fragments arranged by size:
3309 938 129
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BbsI BbvCI BcgI BglI BmgBI BmtI BpmI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PpuMI
PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI