Restriction map for
pF1KA1646
(Circular) (Six-base) MAPSORT of: pF1KA1646.seq Check: 2028 from: 1 to: 4724
pF1KA1646 4724 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 399 399
Size: 4724
AatII G_ACGT'C
Cuts at: 490 490
Size: 4724
AccI GT'mk_AC
Cuts at: 758 1762 758
Size: 1004 3720
Acc65I G'GTAC_C
Cuts at: 1740 1740
Size: 4724
AclI AA'CG_TT
Cuts at: 4564 4564
Size: 4724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 2981 3334 3996 2549
Size: 432 353 662 3277
Fragments arranged by size:
3277 662 432 353
AfeI AGC'GCT
Cuts at: 166 1543 166
Size: 1377 3347
AflIII A'CryG_T
Cuts at: 1292 2076 3876 4157 1292
Size: 784 1800 281 1859
Fragments arranged by size:
1859 1800 784 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1649 1649
Size: 4724
AlwNI CAG_nnn'CTG
Cuts at: 462 1188 1601 2029 3467 462
Size: 726 413 428 1438 1719
Fragments arranged by size:
1719 1438 726 428 413
ApaI G_GGCC'C
Cuts at: 227 227
Size: 4724
ApaLI G'TGCA_C
Cuts at: 3562 3562
Size: 4724
ApoI r'AATT_y
Cuts at: 1477 1728 4077 4333 1477
Size: 251 2349 256 1868
Fragments arranged by size:
2349 1868 256 251
AseI AT'TA_AT
Cuts at: 20 20
Size: 4724
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
AvaI C'yCGr_G
Cuts at: 219 773 1744 219
Size: 554 971 3199
Fragments arranged by size:
3199 971 554
BamHI G'GATC_C
Cuts at: 1749 1749
Size: 4724
BanI G'GyrC_C
Cuts at: 235 389 570 856 1740 2154 2402 2437 235
Size: 154 181 286 884 414 248 35 2522
Fragments arranged by size:
2522 884 414 286 248 181 154 35
BanII G_rGCy'C
Cuts at: 188 198 220 227 1318 1738 2768 188
Size: 10 22 7 1091 420 1030 2144
Fragments arranged by size:
2144 1091 1030 420 22 10 7
BbeI G_GCGC'C
Cuts at: 239 2158 2406 239
Size: 1919 248 2557
Fragments arranged by size:
2557 1919 248
BbsI GAAGACnn'nnnn_
Cuts at: 1349 1349
Size: 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 857 891 857
Size: 34 4690
BciVI GTATCCnnnnn_n'
Cuts at: 2622 3678 4724 2622
Size: 1056 1046 2622
Fragments arranged by size:
2622 1056 1046
BclI T'GATC_A
Cuts at: 2244 2244
Size: 4724
BglI GCCn_nnn'nGGC
Cuts at: 112 1322 112
Size: 1210 3514
BglII A'GATC_T
Cuts at: 2239 4276 2239
Size: 2037 2687
BlpI GC'TnA_GC
Cuts at: 1836 1836
Size: 4724
Bme1580I G_kGCm'C
Cuts at: 227 859 1553 2349 2442 3566 227
Size: 632 694 796 93 1124 1385
Fragments arranged by size:
1385 1124 796 694 632 93
BmgBI CAC'GTC
Cuts at: 1239 1239
Size: 4724
BmrI ACTGGGnnnn_n'
Cuts at: 785 1936 2017 2352 4496 785
Size: 1151 81 335 2144 1013
Fragments arranged by size:
2144 1151 1013 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 4724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 496 1210 1371 202
Size: 294 714 161 3555
Fragments arranged by size:
3555 714 294 161
Bpu10I CC'TnA_GC
Cuts at: 585 585
Size: 4724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 1900 2779 3493 3791 1507
Size: 393 879 714 298 2440
Fragments arranged by size:
2440 879 714 393 298
BsaI GGTCTCn'nnnn_
Cuts at: 4428 4428
Size: 4724
BsaAI yAC'GTr
Cuts at: 2077 2077
Size: 4724
BsaBI GATnn'nnATC
Cuts at: 6 808 2238 4275 6
Size: 802 1430 2037 455
Fragments arranged by size:
2037 1430 802 455
BsaHI Gr'CG_yC
Cuts at: 236 487 2155 2403 3105 4601 236
Size: 251 1668 248 702 1496 359
Fragments arranged by size:
1668 1496 702 359 251 248
BsaWI w'CCGG_w
Cuts at: 800 1376 1913 2434 3112 3523 3670 3944 800
Size: 576 537 521 678 411 147 274 1580
Fragments arranged by size:
1580 678 576 537 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 631 661 424
Size: 30 177 30 4487
Fragments arranged by size:
4487 177 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 759 1207 1210 1258 445
Size: 314 448 3 48 3911
Fragments arranged by size:
3911 448 314 48 3
BseYI C'CCAG_C
Cuts at: 969 2149 2907 3572 3980 969
Size: 1180 758 665 408 1713
Fragments arranged by size:
1713 1180 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1091 1566 1609 703
Size: 388 475 43 3818
Fragments arranged by size:
3818 475 388 43
BsiEI CG_ry'CG
Cuts at: 104 2266 2312 3542 104
Size: 2162 46 1230 1286
Fragments arranged by size:
2162 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 384 1738 2516 2706 3566 384
Size: 1354 778 190 860 1542
Fragments arranged by size:
1542 1354 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1343 1593 1343
Size: 250 4474
Bsp1286I G_dGCh'C
Cuts at: 188 198 220 227 384 859 1318 1553 1738
Size: 10 22 7 157 475 459 235 185
Cuts at: 1738 2349 2442 2516 2706 2768 3566 188
Size: 611 93 74 190 62 798 1346
Fragments arranged by size:
1346 798 611 475 459 235 190 185
157 93 74 62 22 10 7
BspEI T'CCGG_A
Cuts at: 800 1376 800
Size: 576 4148
BspHI T'CATG_A
Cuts at: 3156 3156
Size: 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 399 1774 1988 2290 2671 399
Size: 1375 214 302 381 2452
Fragments arranged by size:
2452 1375 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3016 3070 4554 4719 50
Size: 2966 54 1484 165 55
Fragments arranged by size:
2966 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2636 2636
Size: 4724
BsrFI r'CCGG_y
Cuts at: 232 2722 2903 232
Size: 2490 181 2053
Fragments arranged by size:
2490 2053 181
BssSI C'ACGA_G
Cuts at: 1679 2995 3703 1679
Size: 1316 708 2700
Fragments arranged by size:
2700 1316 708
BstAPI GCAn_nnn'nTGC
Cuts at: 144 462 2101 144
Size: 318 1639 2767
Fragments arranged by size:
2767 1639 318
BstBI TT'CG_AA
Cuts at: 3085 3085
Size: 4724
BstYI r'GATC_y
Cuts at: 1 1123 1749 2216 2239 2574 2820 3126 3138
Size: 1122 626 467 23 335 246 306 12
Cuts at: 3138 3224 4276 1
Size: 86 1052 449
Fragments arranged by size:
1122 1052 626 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 851 911 107
Size: 744 60 3920
Fragments arranged by size:
3920 744 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 2674 2855 3018 264
Size: 2410 181 163 1970
Fragments arranged by size:
2410 1970 181 163
BtsI GCAGTG_nn'
Cuts at: 327 1074 1653 1967 327
Size: 747 579 314 3084
Fragments arranged by size:
3084 747 579 314
DraI TTT'AAA
Cuts at: 1055 1723 1055
Size: 668 4056
DraIII CAC_nnn'GTG
Cuts at: 1119 1119
Size: 4724
DrdI GACnn_nn'nnGTC
Cuts at: 679 1618 2430 3774 679
Size: 939 812 1344 1629
Fragments arranged by size:
1629 1344 939 812
EaeI y'GGCC_r
Cuts at: 908 1274 2309 2483 2874 2901 4246 908
Size: 366 1035 174 391 27 1345 1386
Fragments arranged by size:
1386 1345 1035 391 366 174 27
EagI C'GGCC_G
Cuts at: 2309 2309
Size: 4724
EarI CTCTTCn'nnn_
Cuts at: 1685 2747 2957 1685
Size: 1062 210 3452
Fragments arranged by size:
3452 1062 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 228 707 2932 3674 3820 4534 142
Size: 86 479 2225 742 146 714 332
Fragments arranged by size:
2225 742 714 479 332 146 86
EcoICRI GAG'CTC
Cuts at: 1736 1736
Size: 4724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 496 1210 1371 2549 2981 3334 3996 202
Size: 294 714 161 1178 432 353 662 930
Fragments arranged by size:
1178 930 714 662 432 353 294 161
EcoNI CCTnn'n_nnAGG
Cuts at: 1525 1525
Size: 4724
EcoO109I rG'GnC_Cy
Cuts at: 223 1323 1620 1863 223
Size: 1100 297 243 3084
Fragments arranged by size:
3084 1100 297 243
EcoRI G'AATT_C
Cuts at: 1728 1728
Size: 4724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1560 2743 1560
Size: 1183 3541
FspI TGC'GCA
Cuts at: 2505 2505
Size: 4724
HaeII r_GCGC'y
Cuts at: 168 239 752 883 1545 2158 2406 3636 4407
Size: 71 513 131 662 613 248 1230 771
Cuts at: 4407 168
Size: 485
Fragments arranged by size:
1230 771 662 613 513 485 248 131
71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 907 939 1397 1429 2566 2596 2598
Size: 32 634 32 458 32 1137 30 2
Cuts at: 2598 2628 241
Size: 30 2337
Fragments arranged by size:
2337 1137 634 458 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 759 827 1763 759
Size: 68 936 3720
Fragments arranged by size:
3720 936 68
KasI G'GCGC_C
Cuts at: 235 2154 2402 235
Size: 1919 248 2557
Fragments arranged by size:
2557 1919 248
KpnI G_GTAC'C
Cuts at: 1744 1744
Size: 4724
MluI A'CGCG_T
Cuts at: 4157 4157
Size: 4724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1616 3483 3667 1616
Size: 1867 184 2673
Fragments arranged by size:
2673 1867 184
MscI TGG'CCA
Cuts at: 910 1276 2485 910
Size: 366 1209 3149
Fragments arranged by size:
3149 1209 366
MslI CAynn'nnrTG
Cuts at: 916 1079 1291 2713 2840 916
Size: 163 212 1422 127 2800
Fragments arranged by size:
2800 1422 212 163 127
MspA1I CmG'CkG
Cuts at: 135 395 438 1188 1662 1835 2149 2509 3293
Size: 260 43 750 474 173 314 360 784
Cuts at: 3293 3538 135
Size: 245 1321
Fragments arranged by size:
1321 784 750 474 360 314 260 245
173 43
NaeI GCC'GGC
Cuts at: 234 2905 234
Size: 2671 2053
NarI GG'CG_CC
Cuts at: 236 2155 2403 236
Size: 1919 248 2557
Fragments arranged by size:
2557 1919 248
NcoI C'CATG_G
Cuts at: 107 911 107
Size: 804 3920
NgoMIV G'CCGG_C
Cuts at: 232 2903 232
Size: 2671 2053
NspI r_CATG'y
Cuts at: 625 1296 1777 2277 3880 625
Size: 671 481 500 1603 1469
Fragments arranged by size:
1603 1469 671 500 481
PasI CC'CwG_GG
Cuts at: 1319 1319
Size: 4724
PciI A'CATG_T
Cuts at: 1292 3876 1292
Size: 2584 2140
PfoI T'CCnGG_A
Cuts at: 1600 1600
Size: 4724
PmeI GTTT'AAAC
Cuts at: 1723 1723
Size: 4724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 4692
PpuMI rG'GwC_Cy
Cuts at: 1620 1620
Size: 4724
PspOMI G'GGCC_C
Cuts at: 223 223
Size: 4724
PstI C_TGCA'G
Cuts at: 458 1771 2456 458
Size: 1313 685 2726
Fragments arranged by size:
2726 1313 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4724
PvuII CAG'CTG
Cuts at: 438 1188 1662 2149 2509 438
Size: 750 474 487 360 2653
Fragments arranged by size:
2653 750 487 474 360
RsrII CG'GwC_CG
Cuts at: 2919 2919
Size: 4724
SacI G_AGCT'C
Cuts at: 1738 1738
Size: 4724
SalI G'TCGA_C
Cuts at: 757 1761 757
Size: 1004 3720
SapI GCTCTTCn'nnn_
Cuts at: 2747 2957 2747
Size: 210 4514
SbfI CC_TGCA'GG
Cuts at: 1771 1771
Size: 4724
ScaI AGT'ACT
Cuts at: 4241 4241
Size: 4724
SfcI C'TryA_G
Cuts at: 33 454 1767 2452 3420 3611 33
Size: 421 1313 685 968 191 1146
Fragments arranged by size:
1313 1146 968 685 421 191
SfoI GGC'GCC
Cuts at: 237 2156 2404 237
Size: 1919 248 2557
Fragments arranged by size:
2557 1919 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
SmaI CCC'GGG
Cuts at: 221 775 1746 221
Size: 554 971 3199
Fragments arranged by size:
3199 971 554
SmlI C'TyrA_G
Cuts at: 1522 1879 2794 3508 3770 1522
Size: 357 915 714 262 2476
Fragments arranged by size:
2476 915 714 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4724
SphI G_CATG'C
Cuts at: 1777 2277 1777
Size: 500 4224
StyI C'CwwG_G
Cuts at: 107 635 911 1129 1858 2212 107
Size: 528 276 218 729 354 2619
Fragments arranged by size:
2619 729 528 354 276 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 2307 2440 2899 3108 141
Size: 2166 133 459 209 1757
Fragments arranged by size:
2166 1757 459 209 133
TatI w'GTAC_w
Cuts at: 4239 4239
Size: 4724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 823 855 4241 4273 424
Size: 32 367 32 3386 32 875
Fragments arranged by size:
3386 875 367 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1092 2521 1092
Size: 1429 3295
XbaI T'CTAG_A
Cuts at: 1755 1755
Size: 4724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2219 2219
Size: 4724
XmaI C'CCGG_G
Cuts at: 219 773 1744 219
Size: 554 971 3199
Fragments arranged by size:
3199 971 554
ZraI GAC'GTC
Cuts at: 488 488
Size: 4724
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI