Restriction map for
pF1KA1633
(Circular) (Six-base) MAPSORT of: pF1KA1633.seq Check: 9958 from: 1 to: 8555
pF1KA1633 8555 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 508 561 508
Size: 53 8502
AatII G_ACGT'C
Cuts at: 137 137
Size: 8555
AccI GT'mk_AC
Cuts at: 2110 2197 5593 2110
Size: 87 3396 5072
Fragments arranged by size:
5072 3396 87
Acc65I G'GTAC_C
Cuts at: 5571 5571
Size: 8555
AclI AA'CG_TT
Cuts at: 3121 8395 3121
Size: 5274 3281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 533 1226 1570 2149 2674 3052 3104 3320
Size: 67 693 344 579 525 378 52 216
Cuts at: 3320 3335 4151 4241 4573 4747 4810 5281 6380
Size: 15 816 90 332 174 63 471 1099
Cuts at: 6380 6812 7165 7827 466
Size: 432 353 662 1194
Fragments arranged by size:
1194 1099 816 693 662 579 525 471
432 378 353 344 332 216 174 90
67 63 52 15
AflII C'TTAA_G
Cuts at: 2065 2352 2065
Size: 287 8268
AflIII A'CryG_T
Cuts at: 1577 3651 5907 7707 7988 1577
Size: 2074 2256 1800 281 2144
Fragments arranged by size:
2256 2144 2074 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2421 2421
Size: 8555
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5163 5195 5514 5546 5163
Size: 32 319 32 8172
Fragments arranged by size:
8172 319 32 32
AlwNI CAG_nnn'CTG
Cuts at: 161 1329 2127 3723 4671 4704 4743 5860 7298
Size: 1168 798 1596 948 33 39 1117 1438
Cuts at: 7298 161
Size: 1418
Fragments arranged by size:
1596 1438 1418 1168 1117 948 798 39
33
ApaI G_GGCC'C
Cuts at: 2394 3567 2394
Size: 1173 7382
ApaLI G'TGCA_C
Cuts at: 7393 7393
Size: 8555
ApoI r'AATT_y
Cuts at: 379 1192 1591 1914 1949 3489 3759 4310 4393
Size: 813 399 323 35 1540 270 551 83
Cuts at: 4393 4460 5559 7908 8164 379
Size: 67 1099 2349 256 770
Fragments arranged by size:
2349 1540 1099 813 770 551 399 323
270 256 83 67 35
AseI AT'TA_AT
Cuts at: 20 2270 3416 20
Size: 2250 1146 5159
Fragments arranged by size:
5159 2250 1146
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8555
AvaI C'yCGr_G
Cuts at: 5287 5575 5287
Size: 288 8267
BamHI G'GATC_C
Cuts at: 980 4513 5580 980
Size: 3533 1067 3955
Fragments arranged by size:
3955 3533 1067
BanI G'GyrC_C
Cuts at: 2850 5571 5985 6233 6268 2850
Size: 2721 414 248 35 5137
Fragments arranged by size:
5137 2721 414 248 35
BanII G_rGCy'C
Cuts at: 425 884 1661 2394 3331 3567 3866 5514 5569
Size: 459 777 733 937 236 299 1648 55
Cuts at: 5569 6599 425
Size: 1030 2381
Fragments arranged by size:
2381 1648 1030 937 777 733 459 299
236 55
BbeI G_GCGC'C
Cuts at: 5989 6237 5989
Size: 248 8307
BbsI GAAGACnn'nnnn_
Cuts at: 1214 1489 1930 2587 4127 4258 4573 1214
Size: 275 441 657 1540 131 315 5196
Fragments arranged by size:
5196 1540 657 441 315 275 131
BciVI GTATCCnnnnn_n'
Cuts at: 2341 6453 7509 8555 2341
Size: 4112 1056 1046 2341
Fragments arranged by size:
4112 2341 1056 1046
BclI T'GATC_A
Cuts at: 1541 1989 3231 3399 5442 6075 1541
Size: 448 1242 168 2043 633 4021
Fragments arranged by size:
4021 2043 1242 633 448 168
BglI GCCn_nnn'nGGC
Cuts at: 631 1165 1246 2859 631
Size: 534 81 1613 6327
Fragments arranged by size:
6327 1613 534 81
BglII A'GATC_T
Cuts at: 579 3008 3813 6070 8107 579
Size: 2429 805 2257 2037 1027
Fragments arranged by size:
2429 2257 2037 1027 805
BlpI GC'TnA_GC
Cuts at: 154 507 3134 4025 4705 5413 5667 154
Size: 353 2627 891 680 708 254 3042
Fragments arranged by size:
3042 2627 891 708 680 353 254
Bme1580I G_kGCm'C
Cuts at: 2394 2699 3567 5305 6180 6273 7397 2394
Size: 305 868 1738 875 93 1124 3552
Fragments arranged by size:
3552 1738 1124 875 868 305 93
BmgBI CAC'GTC
Cuts at: 1578 1578
Size: 8555
BmrI ACTGGGnnnn_n'
Cuts at: 173 3513 5767 5848 6183 8327 173
Size: 3340 2254 81 335 2144 401
Fragments arranged by size:
3340 2254 2144 401 335 81
BmtI G_CTAG'C
Cuts at: 2225 2225
Size: 8555
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3273 4573 4575 5299 6922 3273
Size: 1300 2 724 1623 4906
Fragments arranged by size:
4906 1623 1300 724 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 896 1381 1393 1909 2573 3228 3724 3745
Size: 454 485 12 516 664 655 496 21
Cuts at: 3745 4054 4545 4600 5292 5512 442
Size: 309 491 55 692 220 3485
Fragments arranged by size:
3485 692 664 655 516 496 491 485
454 309 220 55 21 12
Bpu10I CC'TnA_GC
Cuts at: 1660 2588 4160 5497 1660
Size: 928 1572 1337 4718
Fragments arranged by size:
4718 1572 1337 928
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 409 1675 2039 3597 4265 4491 5731 6610
Size: 30 1266 364 1558 668 226 1240 879
Cuts at: 6610 7324 7622 379
Size: 714 298 1312
Fragments arranged by size:
1558 1312 1266 1240 879 714 668 364
298 226 30
BsaI GGTCTCn'nnnn_
Cuts at: 4217 8259 4217
Size: 4042 4513
BsaAI yAC'GTr
Cuts at: 3645 3654 5908 3645
Size: 9 2254 6292
Fragments arranged by size:
6292 2254 9
BsaBI GATnn'nnATC
Cuts at: 6 6069 8106 6
Size: 6063 2037 455
Fragments arranged by size:
6063 2037 455
BsaHI Gr'CG_yC
Cuts at: 134 5986 6234 6936 8432 134
Size: 5852 248 702 1496 257
Fragments arranged by size:
5852 1496 702 257 248
BsaWI w'CCGG_w
Cuts at: 5744 6265 6943 7354 7501 7775 5744
Size: 521 678 411 147 274 6524
Fragments arranged by size:
6524 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 249 279 4714 4744 4933 4939 4963 4969 4971
Size: 30 4435 30 189 6 24 6 2
Cuts at: 4971 5001 5093 5123 249
Size: 30 92 30 3681
Fragments arranged by size:
4435 3681 189 92 30 30 30 30
24 6 6 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 793 847 1948 3967 4735 4913 793
Size: 54 1101 2019 768 178 4435
Fragments arranged by size:
4435 2019 1101 768 178 54
BseYI C'CCAG_C
Cuts at: 217 2672 5146 5166 5187 5533 5980 6738 7403
Size: 2455 2474 20 21 346 447 758 665
Cuts at: 7403 7811 217
Size: 408 961
Fragments arranged by size:
2474 2455 961 758 665 447 408 346
21 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 920 1392 2746 2795 2827 3543 4735 586
Size: 334 472 1354 49 32 716 1192 4406
Fragments arranged by size:
4406 1354 1192 716 472 334 49 32
BsiEI CG_ry'CG
Cuts at: 104 6097 6143 7373 104
Size: 5993 46 1230 1286
Fragments arranged by size:
5993 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 279 425 884 1661 1693 3866 4586 4820 5224
Size: 146 459 777 32 2173 720 234 404
Cuts at: 5224 5569 6347 6537 7397 279
Size: 345 778 190 860 1437
Fragments arranged by size:
2173 1437 860 778 777 720 459 404
345 234 190 146 32
BsmI GAATG_Cn'
Cuts at: 374 2422 3135 374
Size: 2048 713 5794
Fragments arranged by size:
5794 2048 713
BsmBI CGTCTCn'nnnn_
Cuts at: 658 2971 658
Size: 2313 6242
Bsp1286I G_dGCh'C
Cuts at: 279 425 884 1661 1693 2394 2699 3331 3567
Size: 146 459 777 32 701 305 632 236
Cuts at: 3567 3866 4586 4820 5224 5305 5514 5569 6180
Size: 299 720 234 404 81 209 55 611
Cuts at: 6180 6273 6347 6537 6599 7397 279
Size: 93 74 190 62 798 1437
Fragments arranged by size:
1437 798 777 720 701 632 611 459
404 305 299 236 234 209 190 146
93 81 74 62 55 32
BspHI T'CATG_A
Cuts at: 6987 6987
Size: 8555
BspMI ACCTGCnnnn'nnnn_
Cuts at: 508 561 1853 2441 2759 2849 5605 5819 6121
Size: 53 1292 588 318 90 2756 214 302
Cuts at: 6121 6502 508
Size: 381 2561
Fragments arranged by size:
2756 2561 1292 588 381 318 302 214
90 53
BsrBI CCG'CTC
Cuts at: 50 6847 6901 8385 8550 50
Size: 6797 54 1484 165 55
Fragments arranged by size:
6797 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1455 1481 4505 6467 1455
Size: 26 3024 1962 3543
Fragments arranged by size:
3543 3024 1962 26
BsrFI r'CCGG_y
Cuts at: 6553 6734 6553
Size: 181 8374
BsrGI T'GTAC_A
Cuts at: 2607 2607
Size: 8555
BssSI C'ACGA_G
Cuts at: 724 4220 6826 7534 724
Size: 3496 2606 708 1745
Fragments arranged by size:
3496 2606 1745 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1899 2850 3636 4743 5932 1899
Size: 951 786 1107 1189 4522
Fragments arranged by size:
4522 1189 1107 951 786
BstBI TT'CG_AA
Cuts at: 4314 6916 4314
Size: 2602 5953
BstEII G'GTnAC_C
Cuts at: 5343 5343
Size: 8555
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2401 3975 2173
Size: 228 1574 6753
Fragments arranged by size:
6753 1574 228
BstYI r'GATC_y
Cuts at: 1 579 980 1650 3008 3785 3813 4387 4513
Size: 578 401 670 1358 777 28 574 126
Cuts at: 4513 5580 6047 6070 6405 6651 6957 6969 7055
Size: 1067 467 23 335 246 306 12 86
Cuts at: 7055 8107 1
Size: 1052 449
Fragments arranged by size:
1358 1067 1052 777 670 578 574 467
449 401 335 306 246 126 86 28
23 12
BstZ17I GTA'TAC
Cuts at: 2111 2111
Size: 8555
BtgI C'CryG_G
Cuts at: 1079 2738 3897 4850 1079
Size: 1659 1159 953 4784
Fragments arranged by size:
4784 1659 1159 953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 754 6505 6686 6849 754
Size: 5751 181 163 2460
Fragments arranged by size:
5751 2460 181 163
BtsI GCAGTG_nn'
Cuts at: 171 2807 3095 3531 4901 5220 5798 171
Size: 2636 288 436 1370 319 578 2928
Fragments arranged by size:
2928 2636 1370 578 436 319 288
DraI TTT'AAA
Cuts at: 3042 5554 3042
Size: 2512 6043
DraIII CAC_nnn'GTG
Cuts at: 2850 3636 4991 2850
Size: 786 1355 6414
Fragments arranged by size:
6414 1355 786
DrdI GACnn_nn'nnGTC
Cuts at: 139 3402 6261 7605 139
Size: 3263 2859 1344 1089
Fragments arranged by size:
3263 2859 1344 1089
EaeI y'GGCC_r
Cuts at: 2735 2830 3894 3989 4695 5013 5040 5225 5295
Size: 95 1064 95 706 318 27 185 70
Cuts at: 5295 6140 6314 6705 6732 8077 2735
Size: 845 174 391 27 1345 3213
Fragments arranged by size:
3213 1345 1064 845 706 391 318 185
174 95 95 70 27 27
EagI C'GGCC_G
Cuts at: 6140 6140
Size: 8555
EarI CTCTTCn'nnn_
Cuts at: 122 683 793 989 1387 1698 1754 2786 4808
Size: 561 110 196 398 311 56 1032 2022
Cuts at: 4808 5237 6578 6788 122
Size: 429 1341 210 1889
Fragments arranged by size:
2022 1889 1341 1032 561 429 398 311
210 196 110 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6763 7505 7651 8365 6763
Size: 742 146 714 6953
Fragments arranged by size:
6953 742 714 146
EcoICRI GAG'CTC
Cuts at: 423 882 1659 3864 5567 423
Size: 459 777 2205 1703 3411
Fragments arranged by size:
3411 2205 1703 777 459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 466 533 896 1226 1381 1393 1570 1909
Size: 24 67 363 330 155 12 177 339
Cuts at: 1909 2149 2573 2674 3052 3104 3228 3320 3335
Size: 240 424 101 378 52 124 92 15
Cuts at: 3335 3724 3745 4054 4151 4241 4545 4573 4600
Size: 389 21 309 97 90 304 28 27
Cuts at: 4600 4747 4810 5281 5292 5512 6380 6812 7165
Size: 147 63 471 11 220 868 432 353
Cuts at: 7165 7827 442
Size: 662 1170
Fragments arranged by size:
1170 868 662 471 432 424 389 378
363 353 339 330 309 304 240 220
177 155 147 124 101 97 92 90
67 63 52 28 27 24 21 15
12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 829 5062 5242 5473 829
Size: 4233 180 231 3911
Fragments arranged by size:
4233 3911 231 180
EcoO109I rG'GnC_Cy
Cuts at: 999 1167 1368 2390 3563 4488 5076 5694 999
Size: 168 201 1022 1173 925 588 618 3860
Fragments arranged by size:
3860 1173 1022 925 618 588 201 168
EcoRI G'AATT_C
Cuts at: 5559 5559
Size: 8555
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2620 2801 2934 4230 6574 2080
Size: 540 181 133 1296 2344 4061
Fragments arranged by size:
4061 2344 1296 540 181 133
FspI TGC'GCA
Cuts at: 6336 6336
Size: 8555
HaeII r_GCGC'y
Cuts at: 5424 5989 6237 7467 8238 5424
Size: 565 248 1230 771 5741
Fragments arranged by size:
5741 1230 771 565 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 823 837 853 855 869 885 1533 1565 1981
Size: 14 16 2 14 16 648 32 416
Cuts at: 1981 2013 3777 3809 4933 4939 4965 4971 5093
Size: 32 1764 32 1124 6 26 6 122
Cuts at: 5093 5125 6397 6427 6429 6459 823
Size: 32 1272 30 2 30 2919
Fragments arranged by size:
2919 1764 1272 1124 648 416 122 32
32 32 32 30 30 26 16 16
14 14 6 6 2 2
HincII GTy'rAC
Cuts at: 3922 5594 3922
Size: 1672 6883
HindIII A'AGCT_T
Cuts at: 700 809 5263 5401 700
Size: 109 4454 138 3854
Fragments arranged by size:
4454 3854 138 109
KasI G'GCGC_C
Cuts at: 5985 6233 5985
Size: 248 8307
KpnI G_GTAC'C
Cuts at: 5575 5575
Size: 8555
MfeI C'AATT_G
Cuts at: 5446 5446
Size: 8555
MluI A'CGCG_T
Cuts at: 7988 7988
Size: 8555
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 104 4053 4355 7314 7498 104
Size: 3949 302 2959 184 1161
Fragments arranged by size:
3949 2959 1161 302 184
MscI TGG'CCA
Cuts at: 2737 3896 5015 5297 6316 2737
Size: 1159 1119 282 1019 4976
Fragments arranged by size:
4976 1159 1119 1019 282
MslI CAynn'nnrTG
Cuts at: 2564 6544 6671 2564
Size: 3980 127 4448
Fragments arranged by size:
4448 3980 127
MspA1I CmG'CkG
Cuts at: 158 474 537 2997 3672 3723 4011 4593 4671
Size: 316 63 2460 675 51 288 582 78
Cuts at: 4671 4688 4694 4743 5412 5666 5980 6340 7124
Size: 17 6 49 669 254 314 360 784
Cuts at: 7124 7369 158
Size: 245 1344
Fragments arranged by size:
2460 1344 784 675 669 582 360 316
314 288 254 245 78 63 51 49
17 6
NaeI GCC'GGC
Cuts at: 6736 6736
Size: 8555
NarI GG'CG_CC
Cuts at: 5986 6234 5986
Size: 248 8307
NcoI C'CATG_G
Cuts at: 1079 3897 4850 1079
Size: 2818 953 4784
Fragments arranged by size:
4784 2818 953
NgoMIV G'CCGG_C
Cuts at: 6734 6734
Size: 8555
NheI G'CTAG_C
Cuts at: 2221 2221
Size: 8555
NspI r_CATG'y
Cuts at: 854 3771 5528 5608 6108 7711 854
Size: 2917 1757 80 500 1603 1698
Fragments arranged by size:
2917 1757 1698 1603 500 80
PasI CC'CwG_GG
Cuts at: 145 145
Size: 8555
PciI A'CATG_T
Cuts at: 7707 7707
Size: 8555
PflMI CCAn_nnn'nTGG
Cuts at: 2985 3012 3462 4933 5052 2985
Size: 27 450 1471 119 6488
Fragments arranged by size:
6488 1471 450 119 27
PfoI T'CCnGG_A
Cuts at: 458 752 4001 4561 4676 5517 458
Size: 294 3249 560 115 841 3496
Fragments arranged by size:
3496 3249 841 560 294 115
PmeI GTTT'AAAC
Cuts at: 5554 5554
Size: 8555
PmlI CAC'GTG
Cuts at: 3654 3654
Size: 8555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7001 7033 7001
Size: 32 8523
PpuMI rG'GwC_Cy
Cuts at: 999 1368 999
Size: 369 8186
PshAI GACnn'nnGTC
Cuts at: 2193 2193
Size: 8555
PspOMI G'GGCC_C
Cuts at: 2390 3563 2390
Size: 1173 7382
PstI C_TGCA'G
Cuts at: 166 1647 1823 1850 3002 3023 3749 3800 3965
Size: 1481 176 27 1152 21 726 51 165
Cuts at: 3965 4634 4693 5602 6287 166
Size: 669 59 909 685 2434
Fragments arranged by size:
2434 1481 1152 909 726 685 669 176
165 59 51 27 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8555
PvuII CAG'CTG
Cuts at: 474 2997 3723 4011 4671 4743 5412 5980 6340
Size: 2523 726 288 660 72 669 568 360
Cuts at: 6340 474
Size: 2689
Fragments arranged by size:
2689 2523 726 669 660 568 360 288
72
RsrII CG'GwC_CG
Cuts at: 3667 6750 3667
Size: 3083 5472
SacI G_AGCT'C
Cuts at: 425 884 1661 3866 5569 425
Size: 459 777 2205 1703 3411
Fragments arranged by size:
3411 2205 1703 777 459
SalI G'TCGA_C
Cuts at: 5592 5592
Size: 8555
SapI GCTCTTCn'nnn_
Cuts at: 683 793 1387 1698 1754 4808 6578 6788 683
Size: 110 594 311 56 3054 1770 210 2450
Fragments arranged by size:
3054 2450 1770 594 311 210 110 56
SbfI CC_TGCA'GG
Cuts at: 1850 5602 1850
Size: 3752 4803
ScaI AGT'ACT
Cuts at: 8072 8072
Size: 8555
SfcI C'TryA_G
Cuts at: 33 162 1028 1171 1643 1819 1846 2198 2998
Size: 129 866 143 472 176 27 352 800
Cuts at: 2998 3019 3745 3796 3961 4630 4689 5250 5598
Size: 21 726 51 165 669 59 561 348
Cuts at: 5598 6283 7251 7442 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 866 800 726 685 669 561
472 352 348 191 176 165 143 129
59 51 27 21
SfoI GGC'GCC
Cuts at: 5987 6235 5987
Size: 248 8307
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8555
SmaI CCC'GGG
Cuts at: 5577 5577
Size: 8555
SmlI C'TyrA_G
Cuts at: 358 424 1654 2054 2065 2352 3576 4280 4470
Size: 66 1230 400 11 287 1224 704 190
Cuts at: 4470 5710 6625 7339 7601 358
Size: 1240 915 714 262 1312
Fragments arranged by size:
1312 1240 1230 1224 915 714 704 400
287 262 190 66 11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8555
SphI G_CATG'C
Cuts at: 5608 6108 5608
Size: 500 8055
StuI AGG'CCT
Cuts at: 649 5248 5502 649
Size: 4599 254 3702
Fragments arranged by size:
4599 3702 254
StyI C'CwwG_G
Cuts at: 904 1079 1928 2699 3897 4850 5689 6043 904
Size: 175 849 771 1198 953 839 354 3416
Fragments arranged by size:
3416 1198 953 849 839 771 354 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1336 2846 2992 3655 4105 5201 6138 6271 6730
Size: 1510 146 663 450 1096 937 133 459
Cuts at: 6730 6939 1336
Size: 209 2952
Fragments arranged by size:
2952 1510 1096 937 663 459 450 209
146 133
TatI w'GTAC_w
Cuts at: 1279 2607 8070 1279
Size: 1328 5463 1764
Fragments arranged by size:
5463 1764 1328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5284 5316 8072 8104 5284
Size: 32 2756 32 5735
Fragments arranged by size:
5735 2756 32 32
Tth111I GACn'n_nGTC
Cuts at: 6352 6352
Size: 8555
XbaI T'CTAG_A
Cuts at: 5586 5586
Size: 8555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 219 2469 2982 5235 5487 6050 114
Size: 105 2250 513 2253 252 563 2619
Fragments arranged by size:
2619 2253 2250 563 513 252 105
XmaI C'CCGG_G
Cuts at: 5575 5575
Size: 8555
XmnI GAAnn'nnTTC
Cuts at: 2513 3320 3805 4397 2513
Size: 807 485 592 6671
Fragments arranged by size:
6671 807 592 485
ZraI GAC'GTC
Cuts at: 135 135
Size: 8555
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AhdI AleI AscI AvrII BaeI BbvCI
BcgI BfrBI BsiWI BspEI BssHII Bsu36I ClaI CspCI
EcoRV FseI FspAI HpaI NdeI NotI NruI NsiI
PacI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI