Restriction map for
pF1KA1624
(Circular) (Six-base) MAPSORT of: pF1KA1624.seq Check: 5263 from: 1 to: 4913
pF1KA1624 4913 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1328 1951 1328
Size: 623 4290
Acc65I G'GTAC_C
Cuts at: 523 1929 523
Size: 1406 3507
AclI AA'CG_TT
Cuts at: 4753 4753
Size: 4913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 531 587 1417 1739 2738 3170 3917 4185
Size: 166 56 830 322 999 432 747 268
Cuts at: 4185 365
Size: 1093
Fragments arranged by size:
1093 999 830 747 432 322 268 166
56
AflIII A'CryG_T
Cuts at: 2265 3369 4346 2265
Size: 1104 977 2832
Fragments arranged by size:
2832 1104 977
AgeI A'CCGG_T
Cuts at: 3301 3301
Size: 4913
AlwNI CAG_nnn'CTG
Cuts at: 204 520 1242 1695 2218 3785 204
Size: 316 722 453 523 1567 1332
Fragments arranged by size:
1567 1332 722 523 453 316
ApaLI G'TGCA_C
Cuts at: 3683 3683
Size: 4913
ApoI r'AATT_y
Cuts at: 884 1741 1917 4266 4522 884
Size: 857 176 2349 256 1275
Fragments arranged by size:
2349 1275 857 256 176
AseI AT'TA_AT
Cuts at: 20 20
Size: 4913
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4913
AvaI C'yCGr_G
Cuts at: 1487 1933 1487
Size: 446 4467
AvrII C'CTAG_G
Cuts at: 151 151
Size: 4913
BamHI G'GATC_C
Cuts at: 1938 1938
Size: 4913
BanI G'GyrC_C
Cuts at: 120 523 1345 1900 1929 2343 2591 2626 120
Size: 403 822 555 29 414 248 35 2407
Fragments arranged by size:
2407 822 555 414 403 248 35 29
BanII G_rGCy'C
Cuts at: 1883 1927 2957 1883
Size: 44 1030 3839
Fragments arranged by size:
3839 1030 44
BbeI G_GCGC'C
Cuts at: 124 1904 2347 2595 124
Size: 1780 443 248 2442
Fragments arranged by size:
2442 1780 443 248
BbsI GAAGACnn'nnnn_
Cuts at: 353 631 1710 1799 353
Size: 278 1079 89 3467
Fragments arranged by size:
3467 1079 278 89
BciVI GTATCCnnnnn_n'
Cuts at: 2811 3572 4913 2811
Size: 761 1341 2811
Fragments arranged by size:
2811 1341 761
BclI T'GATC_A
Cuts at: 2433 2433
Size: 4913
BglI GCCn_nnn'nGGC
Cuts at: 118 118
Size: 4913
BglII A'GATC_T
Cuts at: 1211 2428 4465 1211
Size: 1217 2037 1659
Fragments arranged by size:
2037 1659 1217
BlpI GC'TnA_GC
Cuts at: 2025 2025
Size: 4913
Bme1580I G_kGCm'C
Cuts at: 1288 2538 2631 3687 1288
Size: 1250 93 1056 2514
Fragments arranged by size:
2514 1250 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 522 1354 2125 2206 2541 4685 522
Size: 832 771 81 335 2144 750
Fragments arranged by size:
2144 832 771 750 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3280 3280
Size: 4913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 1219 468
Size: 751 4162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 2089 2968 3460 3758 229
Size: 1860 879 492 298 1384
Fragments arranged by size:
1860 1384 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4617 4617
Size: 4913
BsaAI yAC'GTr
Cuts at: 2266 2266
Size: 4913
BsaBI GATnn'nnATC
Cuts at: 6 952 2427 4464 6
Size: 946 1475 2037 455
Fragments arranged by size:
2037 1475 946 455
BsaHI Gr'CG_yC
Cuts at: 121 1901 2344 2592 3294 4790 121
Size: 1780 443 248 702 1496 244
Fragments arranged by size:
1780 1496 702 443 248 244
BsaWI w'CCGG_w
Cuts at: 2102 2623 3301 3575 3722 4133 2102
Size: 521 678 274 147 411 2882
Fragments arranged by size:
2882 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1048 1051 1078 1081 1048
Size: 3 27 3 4880
Fragments arranged by size:
4880 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1056 1294 1639 1056
Size: 238 345 4330
Fragments arranged by size:
4330 345 238
BseYI C'CCAG_C
Cuts at: 186 1091 1169 1546 2338 3096 3673 4169 186
Size: 905 78 377 792 758 577 496 930
Fragments arranged by size:
930 905 792 758 577 496 377 78
BsiEI CG_ry'CG
Cuts at: 104 2455 2501 3709 104
Size: 2351 46 1208 1308
Fragments arranged by size:
2351 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1927 2705 2895 3687 1927
Size: 778 190 792 3153
Fragments arranged by size:
3153 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1288 1883 1927 2538 2631 2705 2895 2957 3687
Size: 595 44 611 93 74 190 62 730
Cuts at: 3687 1288
Size: 2514
Fragments arranged by size:
2514 730 611 595 190 93 74 62
44
BspEI T'CCGG_A
Cuts at: 4133 4133
Size: 4913
BspHI T'CATG_A
Cuts at: 1217 4089 1217
Size: 2872 2041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1963 2177 2479 2860 1963
Size: 214 302 381 4016
Fragments arranged by size:
4016 381 302 214
BsrBI CCG'CTC
Cuts at: 50 115 3205 3259 4743 4908 50
Size: 65 3090 54 1484 165 55
Fragments arranged by size:
3090 1484 165 65 55 54
BsrDI GCAATG_nn'
Cuts at: 1148 1637 2825 1148
Size: 489 1188 3236
Fragments arranged by size:
3236 1188 489
BsrFI r'CCGG_y
Cuts at: 2911 3092 3301 2911
Size: 181 209 4523
Fragments arranged by size:
4523 209 181
BssSI C'ACGA_G
Cuts at: 3184 3542 3184
Size: 358 4555
BstAPI GCAn_nnn'nTGC
Cuts at: 2290 2290
Size: 4913
BstBI TT'CG_AA
Cuts at: 960 3274 960
Size: 2314 2599
BstEII G'GTnAC_C
Cuts at: 1860 1860
Size: 4913
BstXI CCAn_nnnn'nTGG
Cuts at: 1367 1546 1673 1367
Size: 179 127 4607
Fragments arranged by size:
4607 179 127
BstYI r'GATC_y
Cuts at: 1 1211 1938 2405 2428 2763 3009 4021 4107
Size: 1210 727 467 23 335 246 1012 86
Cuts at: 4107 4119 4465 1
Size: 12 346 449
Fragments arranged by size:
1210 1012 727 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 144 161 674 1874 1904 107
Size: 37 17 513 1200 30 3116
Fragments arranged by size:
3116 1200 513 37 30 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2863 3044 3207 2863
Size: 181 163 4569
Fragments arranged by size:
4569 181 163
BtsI GCAGTG_nn'
Cuts at: 722 2156 722
Size: 1434 3479
DraI TTT'AAA
Cuts at: 440 984 1912 440
Size: 544 928 3441
Fragments arranged by size:
3441 928 544
DrdI GACnn_nn'nnGTC
Cuts at: 2619 3477 2619
Size: 858 4055
EaeI y'GGCC_r
Cuts at: 110 158 206 1706 2498 2672 3063 3090 4435
Size: 48 48 1500 792 174 391 27 1345
Cuts at: 4435 110
Size: 588
Fragments arranged by size:
1500 1345 792 588 391 174 48 48
27
EagI C'GGCC_G
Cuts at: 2498 2498
Size: 4913
EarI CTCTTCn'nnn_
Cuts at: 1144 1360 1436 2936 3146 1144
Size: 216 76 1500 210 2911
Fragments arranged by size:
2911 1500 216 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3121 3431 3577 4723 3121
Size: 310 146 1146 3311
Fragments arranged by size:
3311 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1925 1925
Size: 4913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 468 531 587 1219 1417 1739 2738 3170
Size: 103 63 56 632 198 322 999 432
Cuts at: 3170 3917 4185 365
Size: 747 268 1093
Fragments arranged by size:
1093 999 747 632 432 322 268 198
103 63 56
EcoNI CCTnn'n_nnAGG
Cuts at: 1085 1085
Size: 4913
EcoO109I rG'GnC_Cy
Cuts at: 1239 1426 2052 1239
Size: 187 626 4100
Fragments arranged by size:
4100 626 187
EcoRI G'AATT_C
Cuts at: 1917 1917
Size: 4913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 561 781 2932 561
Size: 220 2151 2542
Fragments arranged by size:
2542 2151 220
FspI TGC'GCA
Cuts at: 2694 2694
Size: 4913
HaeII r_GCGC'y
Cuts at: 124 1135 1904 2347 2595 3617 4596 124
Size: 1011 769 443 248 1022 979 441
Fragments arranged by size:
1022 1011 979 769 443 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1431 1463 2755 2785 2787 2817 1431
Size: 32 1292 30 2 30 3527
Fragments arranged by size:
3527 1292 32 30 30 2
HincII GTy'rAC
Cuts at: 594 1329 1952 594
Size: 735 623 3555
Fragments arranged by size:
3555 735 623
HpaI GTT'AAC
Cuts at: 594 594
Size: 4913
KasI G'GCGC_C
Cuts at: 120 1900 2343 2591 120
Size: 1780 443 248 2442
Fragments arranged by size:
2442 1780 443 248
KpnI G_GTAC'C
Cuts at: 527 1933 527
Size: 1406 3507
MfeI C'AATT_G
Cuts at: 1046 1046
Size: 4913
MluI A'CGCG_T
Cuts at: 4346 4346
Size: 4913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3584 3768 3584
Size: 184 4729
MscI TGG'CCA
Cuts at: 2674 2674
Size: 4913
MslI CAynn'nnrTG
Cuts at: 310 704 910 1671 2902 3029 310
Size: 394 206 761 1231 127 2194
Fragments arranged by size:
2194 1231 761 394 206 127
MspA1I CmG'CkG
Cuts at: 146 163 1558 2024 2338 2698 3711 3956 146
Size: 17 1395 466 314 360 1013 245 1103
Fragments arranged by size:
1395 1103 1013 466 360 314 245 17
NaeI GCC'GGC
Cuts at: 3094 3094
Size: 4913
NarI GG'CG_CC
Cuts at: 121 1901 2344 2592 121
Size: 1780 443 248 2442
Fragments arranged by size:
2442 1780 443 248
NcoI C'CATG_G
Cuts at: 107 674 107
Size: 567 4346
NgoMIV G'CCGG_C
Cuts at: 3092 3092
Size: 4913
NspI r_CATG'y
Cuts at: 1966 2466 3373 1966
Size: 500 907 3506
Fragments arranged by size:
3506 907 500
PasI CC'CwG_GG
Cuts at: 1071 1884 1071
Size: 813 4100
PciI A'CATG_T
Cuts at: 3369 3369
Size: 4913
PflMI CCAn_nnn'nTGG
Cuts at: 237 520 1242 1695 237
Size: 283 722 453 3455
Fragments arranged by size:
3455 722 453 283
PfoI T'CCnGG_A
Cuts at: 1694 1694
Size: 4913
PmeI GTTT'AAAC
Cuts at: 1912 1912
Size: 4913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4881
PpuMI rG'GwC_Cy
Cuts at: 1239 1426 1239
Size: 187 4726
PstI C_TGCA'G
Cuts at: 200 1960 2645 200
Size: 1760 685 2468
Fragments arranged by size:
2468 1760 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4913
PvuII CAG'CTG
Cuts at: 1558 2338 2698 1558
Size: 780 360 3773
Fragments arranged by size:
3773 780 360
RsrII CG'GwC_CG
Cuts at: 3108 3108
Size: 4913
SacI G_AGCT'C
Cuts at: 1927 1927
Size: 4913
SacII CC_GC'GG
Cuts at: 147 164 147
Size: 17 4896
SalI G'TCGA_C
Cuts at: 1327 1950 1327
Size: 623 4290
SapI GCTCTTCn'nnn_
Cuts at: 2936 3146 2936
Size: 210 4703
SbfI CC_TGCA'GG
Cuts at: 1960 1960
Size: 4913
ScaI AGT'ACT
Cuts at: 4430 4430
Size: 4913
SfcI C'TryA_G
Cuts at: 33 196 1956 2641 3634 3825 33
Size: 163 1760 685 993 191 1121
Fragments arranged by size:
1760 1121 993 685 191 163
SfoI GGC'GCC
Cuts at: 122 1902 2345 2593 122
Size: 1780 443 248 2442
Fragments arranged by size:
2442 1780 443 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4913
SmaI CCC'GGG
Cuts at: 1489 1935 1489
Size: 446 4467
SmlI C'TyrA_G
Cuts at: 244 2068 2983 3475 3737 244
Size: 1824 915 492 262 1420
Fragments arranged by size:
1824 1420 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4913
SphI G_CATG'C
Cuts at: 1966 2466 1966
Size: 500 4413
SrfI GCCC'GGGC
Cuts at: 1489 1489
Size: 4913
StuI AGG'CCT
Cuts at: 764 764
Size: 4913
StyI C'CwwG_G
Cuts at: 107 151 567 674 792 1031 2047 2401 107
Size: 44 416 107 118 239 1016 354 2619
Fragments arranged by size:
2619 1016 416 354 239 118 107 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2496 2629 3088 3297 2496
Size: 133 459 209 4112
Fragments arranged by size:
4112 459 209 133
TatI w'GTAC_w
Cuts at: 655 1103 4428 655
Size: 448 3325 1140
Fragments arranged by size:
3325 1140 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1048 1080 1223 1255 4430 4462 1048
Size: 32 143 32 3175 32 1499
Fragments arranged by size:
3175 1499 143 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2710 2710
Size: 4913
XbaI T'CTAG_A
Cuts at: 1944 1944
Size: 4913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1872 2408 114
Size: 1758 536 2619
Fragments arranged by size:
2619 1758 536
XmaI C'CCGG_G
Cuts at: 1487 1933 1487
Size: 446 4467
XmnI GAAnn'nnTTC
Cuts at: 1585 1585
Size: 4913
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SspI SwaI XhoI ZraI