Restriction map for
pF1KA1622
(Circular) (Six-base) MAPSORT of: pF1KA1622.seq Check: 1904 from: 1 to: 5732
pF1KA1622 5732 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1501 2770 1501
Size: 1269 4463
Acc65I G'GTAC_C
Cuts at: 2748 2748
Size: 5732
AclI AA'CG_TT
Cuts at: 5572 5572
Size: 5732
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1612 2026 2035 3557 3989 4736 5004 1352
Size: 260 414 9 1522 432 747 268 2080
Fragments arranged by size:
2080 1522 747 432 414 268 260 9
AflIII A'CryG_T
Cuts at: 1650 3084 4188 5165 1650
Size: 1434 1104 977 2217
Fragments arranged by size:
2217 1434 1104 977
AgeI A'CCGG_T
Cuts at: 4120 4120
Size: 5732
AhdI GACnn_n'nnGTC
Cuts at: 1801 1801
Size: 5732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1783 1783
Size: 5732
AlwNI CAG_nnn'CTG
Cuts at: 1576 3037 4604 1576
Size: 1461 1567 2704
Fragments arranged by size:
2704 1567 1461
ApaLI G'TGCA_C
Cuts at: 4502 4502
Size: 5732
ApoI r'AATT_y
Cuts at: 692 715 1085 1120 1389 1879 2736 5085 5341
Size: 23 370 35 269 490 857 2349 256
Cuts at: 5341 692
Size: 1083
Fragments arranged by size:
2349 1083 857 490 370 269 256 35
23
AseI AT'TA_AT
Cuts at: 20 874 1816 20
Size: 854 942 3936
Fragments arranged by size:
3936 942 854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5732
AvaI C'yCGr_G
Cuts at: 2752 2752
Size: 5732
BamHI G'GATC_C
Cuts at: 2757 2757
Size: 5732
BanI G'GyrC_C
Cuts at: 2748 3162 3410 3445 2748
Size: 414 248 35 5035
Fragments arranged by size:
5035 414 248 35
BanII G_rGCy'C
Cuts at: 188 2746 3776 188
Size: 2558 1030 2144
Fragments arranged by size:
2558 2144 1030
BbeI G_GCGC'C
Cuts at: 3166 3414 3166
Size: 248 5484
BbsI GAAGACnn'nnnn_
Cuts at: 266 266
Size: 5732
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1739 1773 90
Size: 34 1615 34 4049
Fragments arranged by size:
4049 1615 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1863 3630 4391 5732 1863
Size: 1767 761 1341 1863
Fragments arranged by size:
1863 1767 1341 761
BclI T'GATC_A
Cuts at: 3252 3252
Size: 5732
BfrBI ATG'CAT
Cuts at: 111 493 562 1760 111
Size: 382 69 1198 4083
Fragments arranged by size:
4083 1198 382 69
BglII A'GATC_T
Cuts at: 3247 5284 3247
Size: 2037 3695
BlpI GC'TnA_GC
Cuts at: 2844 2844
Size: 5732
Bme1580I G_kGCm'C
Cuts at: 3357 3450 4506 3357
Size: 93 1056 4583
Fragments arranged by size:
4583 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2944 3025 3360 5504 2944
Size: 81 335 2144 3172
Fragments arranged by size:
3172 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4099 4099
Size: 5732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1079 1415 1468 553
Size: 526 336 53 4817
Fragments arranged by size:
4817 526 336 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1128 2908 3787 4279 4577 205
Size: 923 1780 879 492 298 1360
Fragments arranged by size:
1780 1360 923 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5436 5436
Size: 5732
BsaAI yAC'GTr
Cuts at: 3085 3085
Size: 5732
BsaBI GATnn'nnATC
Cuts at: 6 2033 3246 5283 6
Size: 2027 1213 2037 455
Fragments arranged by size:
2037 2027 1213 455
BsaHI Gr'CG_yC
Cuts at: 3163 3411 4113 5609 3163
Size: 248 702 1496 3286
Fragments arranged by size:
3286 1496 702 248
BsaWI w'CCGG_w
Cuts at: 228 2567 2921 3442 4120 4394 4541 4952 228
Size: 2339 354 521 678 274 147 411 1008
Fragments arranged by size:
2339 1008 678 521 411 354 274 147
BseYI C'CCAG_C
Cuts at: 3157 3915 4492 4988 3157
Size: 758 577 496 3901
Fragments arranged by size:
3901 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3274 3320 4528 104
Size: 3170 46 1208 1308
Fragments arranged by size:
3170 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 539 866 2746 3524 3714 4506 188
Size: 351 327 1880 778 190 792 1414
Fragments arranged by size:
1880 1414 792 778 351 327 190
BsmBI CGTCTCn'nnnn_
Cuts at: 364 364
Size: 5732
Bsp1286I G_dGCh'C
Cuts at: 188 539 866 2746 3357 3450 3524 3714 3776
Size: 351 327 1880 611 93 74 190 62
Cuts at: 3776 4506 188
Size: 730 1414
Fragments arranged by size:
1880 1414 730 611 351 327 190 93
74 62
BspEI T'CCGG_A
Cuts at: 2567 4952 2567
Size: 2385 3347
BspHI T'CATG_A
Cuts at: 1614 1706 4908 1614
Size: 92 3202 2438
Fragments arranged by size:
3202 2438 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 185 1941 2782 2996 3298 3679 185
Size: 1756 841 214 302 381 2238
Fragments arranged by size:
2238 1756 841 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4024 4078 5562 5727 50
Size: 3974 54 1484 165 55
Fragments arranged by size:
3974 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 564 3644 564
Size: 3080 2652
BsrFI r'CCGG_y
Cuts at: 204 3730 3911 4120 204
Size: 3526 181 209 1816
Fragments arranged by size:
3526 1816 209 181
BsrGI T'GTAC_A
Cuts at: 1138 1138
Size: 5732
BssSI C'ACGA_G
Cuts at: 4003 4361 4003
Size: 358 5374
BstAPI GCAn_nnn'nTGC
Cuts at: 2334 3109 2334
Size: 775 4957
BstBI TT'CG_AA
Cuts at: 2421 4093 2421
Size: 1672 4060
BstXI CCAn_nnnn'nTGG
Cuts at: 835 835
Size: 5732
BstYI r'GATC_y
Cuts at: 1 2757 3224 3247 3582 3828 4840 4926 4938
Size: 2756 467 23 335 246 1012 86 12
Cuts at: 4938 5284 1
Size: 346 449
Fragments arranged by size:
2756 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 2634 2689 2634
Size: 55 5677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 148 460 3682 3863 4026 148
Size: 312 3222 181 163 1854
Fragments arranged by size:
3222 1854 312 181 163
BtsI GCAGTG_nn'
Cuts at: 909 1524 2673 2975 909
Size: 615 1149 302 3666
Fragments arranged by size:
3666 1149 615 302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 839 874 2649 2684 839
Size: 35 1775 35 3887
Fragments arranged by size:
3887 1775 35 35
DraI TTT'AAA
Cuts at: 1601 2731 1601
Size: 1130 4602
DrdI GACnn_nn'nnGTC
Cuts at: 550 3438 4296 550
Size: 2888 858 1986
Fragments arranged by size:
2888 1986 858
EaeI y'GGCC_r
Cuts at: 1253 3317 3491 3882 3909 5254 1253
Size: 2064 174 391 27 1345 1731
Fragments arranged by size:
2064 1731 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3317 3317
Size: 5732
EarI CTCTTCn'nnn_
Cuts at: 581 2376 3755 3965 581
Size: 1795 1379 210 2348
Fragments arranged by size:
2348 1795 1379 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 104 199 3940 4250 4396 5542 104
Size: 95 3741 310 146 1146 294
Fragments arranged by size:
3741 1146 310 294 146 95
EcoICRI GAG'CTC
Cuts at: 186 2744 186
Size: 2558 3174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1079 1352 1415 1468 1612 2026 2035 3557
Size: 526 273 63 53 144 414 9 1522
Cuts at: 3557 3989 4736 5004 553
Size: 432 747 268 1281
Fragments arranged by size:
1522 1281 747 526 432 414 273 268
144 63 53 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1201 1201
Size: 5732
EcoO109I rG'GnC_Cy
Cuts at: 174 2871 174
Size: 2697 3035
EcoRI G'AATT_C
Cuts at: 1389 2736 1389
Size: 1347 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2447 2718 3751 2236
Size: 211 271 1033 4217
Fragments arranged by size:
4217 1033 271 211
FspI TGC'GCA
Cuts at: 3513 3513
Size: 5732
HaeII r_GCGC'y
Cuts at: 3166 3414 4436 5415 3166
Size: 248 1022 979 3483
Fragments arranged by size:
3483 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 236 268 321 353 615 647 1787 1819 1938
Size: 32 53 32 262 32 1140 32 119
Cuts at: 1938 1970 3574 3604 3606 3636 236
Size: 32 1604 30 2 30 2332
Fragments arranged by size:
2332 1604 1140 262 119 53 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 437 2655 2771 437
Size: 2218 116 3398
Fragments arranged by size:
3398 2218 116
HindIII A'AGCT_T
Cuts at: 1381 2444 1381
Size: 1063 4669
HpaI GTT'AAC
Cuts at: 437 437
Size: 5732
KasI G'GCGC_C
Cuts at: 3162 3410 3162
Size: 248 5484
KpnI G_GTAC'C
Cuts at: 2752 2752
Size: 5732
MfeI C'AATT_G
Cuts at: 1825 1825
Size: 5732
MluI A'CGCG_T
Cuts at: 5165 5165
Size: 5732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2171 4403 4587 2111
Size: 60 2232 184 3256
Fragments arranged by size:
3256 2232 184 60
MscI TGG'CCA
Cuts at: 3493 3493
Size: 5732
MslI CAynn'nnrTG
Cuts at: 1705 3721 3848 1705
Size: 2016 127 3589
Fragments arranged by size:
3589 2016 127
MspA1I CmG'CkG
Cuts at: 1573 2843 3157 3517 4530 4775 1573
Size: 1270 314 360 1013 245 2530
Fragments arranged by size:
2530 1270 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3913 3913
Size: 5732
NarI GG'CG_CC
Cuts at: 3163 3411 3163
Size: 248 5484
NgoMIV G'CCGG_C
Cuts at: 3911 3911
Size: 5732
NsiI A_TGCA'T
Cuts at: 113 495 564 1762 113
Size: 382 69 1198 4083
Fragments arranged by size:
4083 1198 382 69
NspI r_CATG'y
Cuts at: 415 1654 2785 3285 4192 415
Size: 1239 1131 500 907 1955
Fragments arranged by size:
1955 1239 1131 907 500
PasI CC'CwG_GG
Cuts at: 828 828
Size: 5732
PciI A'CATG_T
Cuts at: 1650 4188 1650
Size: 2538 3194
PfoI T'CCnGG_A
Cuts at: 2361 2361
Size: 5732
PmeI GTTT'AAAC
Cuts at: 2731 2731
Size: 5732
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4871 4903 4871
Size: 32 5700
PpuMI rG'GwC_Cy
Cuts at: 174 174
Size: 5732
PshAI GACnn'nnGTC
Cuts at: 968 968
Size: 5732
PsiI TTA'TAA
Cuts at: 1127 1244 1775 1127
Size: 117 531 5084
Fragments arranged by size:
5084 531 117
PstI C_TGCA'G
Cuts at: 182 470 1593 2779 3464 182
Size: 288 1123 1186 685 2450
Fragments arranged by size:
2450 1186 1123 685 288
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5732
PvuII CAG'CTG
Cuts at: 1573 3157 3517 1573
Size: 1584 360 3788
Fragments arranged by size:
3788 1584 360
RsrII CG'GwC_CG
Cuts at: 207 3927 207
Size: 3720 2012
SacI G_AGCT'C
Cuts at: 188 2746 188
Size: 2558 3174
SalI G'TCGA_C
Cuts at: 2769 2769
Size: 5732
SapI GCTCTTCn'nnn_
Cuts at: 3755 3965 3755
Size: 210 5522
SbfI CC_TGCA'GG
Cuts at: 182 2779 182
Size: 2597 3135
ScaI AGT'ACT
Cuts at: 2628 5249 2628
Size: 2621 3111
SfcI C'TryA_G
Cuts at: 33 178 466 1589 2775 3460 4453 4644 33
Size: 145 288 1123 1186 685 993 191 1121
Fragments arranged by size:
1186 1123 1121 993 685 288 191 145
SfoI GGC'GCC
Cuts at: 3164 3412 3164
Size: 248 5484
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5732
SmaI CCC'GGG
Cuts at: 2754 2754
Size: 5732
SmlI C'TyrA_G
Cuts at: 220 1107 2887 3802 4294 4556 220
Size: 887 1780 915 492 262 1396
Fragments arranged by size:
1780 1396 915 887 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5732
SphI G_CATG'C
Cuts at: 2785 3285 2785
Size: 500 5232
SspI AAT'ATT
Cuts at: 1921 1921
Size: 5732
StyI C'CwwG_G
Cuts at: 315 644 1833 2866 3220 315
Size: 329 1189 1033 354 2827
Fragments arranged by size:
2827 1189 1033 354 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3315 3448 3907 4116 3315
Size: 133 459 209 4931
Fragments arranged by size:
4931 459 209 133
TatI w'GTAC_w
Cuts at: 1138 2626 5247 1138
Size: 1488 2621 1623
Fragments arranged by size:
2621 1623 1488
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 311 343 1178 1210 1221 1253 5249 5281 311
Size: 32 835 32 11 32 3996 32 762
Fragments arranged by size:
3996 835 762 32 32 32 32 11
Tth111I GACn'n_nGTC
Cuts at: 3529 3529
Size: 5732
XbaI T'CTAG_A
Cuts at: 2763 2763
Size: 5732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3227 3227
Size: 5732
XmaI C'CCGG_G
Cuts at: 2752 2752
Size: 5732
XmnI GAAnn'nnTTC
Cuts at: 575 746 1031 2219 2535 575
Size: 171 285 1188 316 3772
Fragments arranged by size:
3772 1188 316 285 171
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI NcoI NdeI NheI
NotI NruI PacI PflMI PmlI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI