Restriction map for
pF1KA1621
(Circular) (Six-base) MAPSORT of: pF1KA1621.seq Check: 488 from: 1 to: 5441
pF1KA1621 5441 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1104 1607 1104
Size: 503 4938
AccI GT'mk_AC
Cuts at: 2171 2479 2171
Size: 308 5133
Acc65I G'GTAC_C
Cuts at: 2457 2457
Size: 5441
AclI AA'CG_TT
Cuts at: 5281 5281
Size: 5441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 2428 3266 3698 4445 4713 1277
Size: 1151 838 432 747 268 2005
Fragments arranged by size:
2005 1151 838 747 432 268
AfeI AGC'GCT
Cuts at: 749 749
Size: 5441
AflIII A'CryG_T
Cuts at: 2793 3897 4874 2793
Size: 1104 977 3360
Fragments arranged by size:
3360 1104 977
AgeI A'CCGG_T
Cuts at: 3829 3829
Size: 5441
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1675 1707 1675
Size: 32 5409
AlwNI CAG_nnn'CTG
Cuts at: 1225 2746 4313 1225
Size: 1521 1567 2353
Fragments arranged by size:
2353 1567 1521
ApaI G_GGCC'C
Cuts at: 2299 2299
Size: 5441
ApaLI G'TGCA_C
Cuts at: 1098 4211 1098
Size: 3113 2328
ApoI r'AATT_y
Cuts at: 658 2445 4794 5050 658
Size: 1787 2349 256 1049
Fragments arranged by size:
2349 1787 1049 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5441
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
AvaI C'yCGr_G
Cuts at: 1838 1893 1942 2461 1838
Size: 55 49 519 4818
Fragments arranged by size:
4818 519 55 49
BamHI G'GATC_C
Cuts at: 2466 2466
Size: 5441
BanI G'GyrC_C
Cuts at: 187 1833 2329 2457 2871 3119 3154 187
Size: 1646 496 128 414 248 35 2474
Fragments arranged by size:
2474 1646 496 414 248 128 35
BanII G_rGCy'C
Cuts at: 1943 2299 2455 3485 1943
Size: 356 156 1030 3899
Fragments arranged by size:
3899 1030 356 156
BbeI G_GCGC'C
Cuts at: 191 2875 3123 191
Size: 2684 248 2509
Fragments arranged by size:
2684 2509 248
BbsI GAAGACnn'nnnn_
Cuts at: 1252 1274 1252
Size: 22 5419
BciVI GTATCCnnnnn_n'
Cuts at: 3339 4100 5441 3339
Size: 761 1341 3339
Fragments arranged by size:
3339 1341 761
BclI T'GATC_A
Cuts at: 2961 2961
Size: 5441
BglII A'GATC_T
Cuts at: 2956 4993 2956
Size: 2037 3404
BlpI GC'TnA_GC
Cuts at: 169 1996 2553 169
Size: 1827 557 3057
Fragments arranged by size:
3057 1827 557
Bme1580I G_kGCm'C
Cuts at: 1102 1838 2299 3066 3159 4215 1102
Size: 736 461 767 93 1056 2328
Fragments arranged by size:
2328 1056 767 736 461 93
BmgBI CAC'GTC
Cuts at: 1125 1125
Size: 5441
BmrI ACTGGGnnnn_n'
Cuts at: 359 2653 2734 3069 5213 359
Size: 2294 81 335 2144 587
Fragments arranged by size:
2294 2144 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 249 3808 249
Size: 3559 1882
Bpu10I CC'TnA_GC
Cuts at: 683 785 683
Size: 102 5339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1912 2617 3496 3988 4286 322
Size: 1590 705 879 492 298 1477
Fragments arranged by size:
1590 1477 879 705 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1623 5145 1623
Size: 3522 1919
BsaAI yAC'GTr
Cuts at: 2085 2794 2085
Size: 709 4732
BsaBI GATnn'nnATC
Cuts at: 6 2955 4992 6
Size: 2949 2037 455
Fragments arranged by size:
2949 2037 455
BsaHI Gr'CG_yC
Cuts at: 188 1766 2310 2872 3120 3822 5318 188
Size: 1578 544 562 248 702 1496 311
Fragments arranged by size:
1578 1496 702 562 544 311 248
BsaWI w'CCGG_w
Cuts at: 534 1231 2630 3151 3829 4103 4250 4661 534
Size: 697 1399 521 678 274 147 411 1314
Fragments arranged by size:
1399 1314 697 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1675 1702 1705 1732 2089 2119 1675
Size: 27 3 27 357 30 4997
Fragments arranged by size:
4997 357 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 730 733 2268 400
Size: 330 3 1535 3573
Fragments arranged by size:
3573 1535 330 3
BseYI C'CCAG_C
Cuts at: 2108 2866 3624 4201 4697 2108
Size: 758 758 577 496 2852
Fragments arranged by size:
2852 758 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1142 1444 1495 1806 2065 2268 874
Size: 268 302 51 311 259 203 4047
Fragments arranged by size:
4047 311 302 268 259 203 51
BsiEI CG_ry'CG
Cuts at: 104 1847 2983 3029 4237 104
Size: 1743 1136 46 1208 1308
Fragments arranged by size:
1743 1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 1102 1332 1412 2065 2090 2225 2455 3233 3423
Size: 230 80 653 25 135 230 778 190
Cuts at: 3423 4215 1102
Size: 792 2328
Fragments arranged by size:
2328 792 778 653 230 230 190 135
80 25
BsmBI CGTCTCn'nnnn_
Cuts at: 903 903
Size: 5441
Bsp1286I G_dGCh'C
Cuts at: 1102 1332 1412 1838 1943 2065 2090 2225 2299
Size: 230 80 426 105 122 25 135 74
Cuts at: 2299 2455 3066 3159 3233 3423 3485 4215 1102
Size: 156 611 93 74 190 62 730 2328
Fragments arranged by size:
2328 730 611 426 230 190 156 135
122 105 93 80 74 74 62 25
BspEI T'CCGG_A
Cuts at: 1231 4661 1231
Size: 3430 2011
BspHI T'CATG_A
Cuts at: 4617 4617
Size: 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1104 1607 2491 2705 3007 3388 1104
Size: 503 884 214 302 381 3157
Fragments arranged by size:
3157 884 503 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2124 3733 3787 5271 5436 50
Size: 2074 1609 54 1484 165 55
Fragments arranged by size:
2074 1609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3353 3353
Size: 5441
BsrFI r'CCGG_y
Cuts at: 3439 3620 3829 3439
Size: 181 209 5051
Fragments arranged by size:
5051 209 181
BssSI C'ACGA_G
Cuts at: 3712 4070 3712
Size: 358 5083
BstAPI GCAn_nnn'nTGC
Cuts at: 2818 2818
Size: 5441
BstBI TT'CG_AA
Cuts at: 1034 3802 1034
Size: 2768 2673
BstEII G'GTnAC_C
Cuts at: 1146 1563 1863 1146
Size: 417 300 4724
Fragments arranged by size:
4724 417 300
BstXI CCAn_nnnn'nTGG
Cuts at: 1875 1875
Size: 5441
BstYI r'GATC_y
Cuts at: 1 1221 2466 2933 2956 3291 3537 4549 4635
Size: 1220 1245 467 23 335 246 1012 86
Cuts at: 4635 4647 4993 1
Size: 12 346 449
Fragments arranged by size:
1245 1220 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1654 2291 1654
Size: 637 4804
BtgI C'CryG_G
Cuts at: 107 1713 1934 107
Size: 1606 221 3614
Fragments arranged by size:
3614 1606 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3391 3572 3735 3391
Size: 181 163 5097
Fragments arranged by size:
5097 181 163
BtsI GCAGTG_nn'
Cuts at: 2684 2684
Size: 5441
DraI TTT'AAA
Cuts at: 2440 2440
Size: 5441
DrdI GACnn_nn'nnGTC
Cuts at: 282 3147 4005 282
Size: 2865 858 1718
Fragments arranged by size:
2865 1718 858
EaeI y'GGCC_r
Cuts at: 1638 1844 2066 2142 3026 3200 3591 3618 4963
Size: 206 222 76 884 174 391 27 1345
Cuts at: 4963 1638
Size: 2116
Fragments arranged by size:
2116 1345 884 391 222 206 174 76
27
EagI C'GGCC_G
Cuts at: 1844 3026 1844
Size: 1182 4259
EarI CTCTTCn'nnn_
Cuts at: 1109 2212 3464 3674 1109
Size: 1103 1252 210 2876
Fragments arranged by size:
2876 1252 1103 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 891 935 3649 3959 4105 5251 891
Size: 44 2714 310 146 1146 1081
Fragments arranged by size:
2714 1146 1081 310 146 44
EcoICRI GAG'CTC
Cuts at: 2453 2453
Size: 5441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 2428 3266 3698 4445 4713 1277
Size: 1151 838 432 747 268 2005
Fragments arranged by size:
2005 1151 838 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 998 998
Size: 5441
EcoO109I rG'GnC_Cy
Cuts at: 200 1590 1674 2295 2296 2336 2580 200
Size: 1390 84 621 1 40 244 3061
Fragments arranged by size:
3061 1390 621 244 84 40 1
EcoRI G'AATT_C
Cuts at: 658 2445 658
Size: 1787 3654
EcoRV GAT'ATC
Cuts at: 1430 1430
Size: 5441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5441
FspI TGC'GCA
Cuts at: 1060 1981 3222 1060
Size: 921 1241 3279
Fragments arranged by size:
3279 1241 921
FspAI rTGC'GCAy
Cuts at: 1060 1981 1060
Size: 921 4520
HaeII r_GCGC'y
Cuts at: 191 751 1532 1706 1744 2875 3123 4145 5124
Size: 560 781 174 38 1131 248 1022 979
Cuts at: 5124 191
Size: 508
Fragments arranged by size:
1131 1022 979 781 560 508 248 174
38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 184 216 745 777 1213 1245 1528
Size: 32 38 32 529 32 436 32 283
Cuts at: 1528 1560 1702 1734 1876 1908 2089 2121 3283
Size: 32 142 32 142 32 181 32 1162
Cuts at: 3283 3313 3315 3345 114
Size: 30 2 30 2210
Fragments arranged by size:
2210 1162 529 436 283 181 142 142
38 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 275 2480 275
Size: 2205 3236
KasI G'GCGC_C
Cuts at: 187 2871 3119 187
Size: 2684 248 2509
Fragments arranged by size:
2684 2509 248
KpnI G_GTAC'C
Cuts at: 2461 2461
Size: 5441
MluI A'CGCG_T
Cuts at: 4874 4874
Size: 5441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 674 4112 4296 674
Size: 3438 184 1819
Fragments arranged by size:
3438 1819 184
MscI TGG'CCA
Cuts at: 3202 3202
Size: 5441
MslI CAynn'nnrTG
Cuts at: 1381 1420 3430 3557 1381
Size: 39 2010 127 3265
Fragments arranged by size:
3265 2010 127 39
MspA1I CmG'CkG
Cuts at: 1715 1800 1923 2279 2552 2866 3226 4239 4484
Size: 85 123 356 273 314 360 1013 245
Cuts at: 4484 1715
Size: 2672
Fragments arranged by size:
2672 1013 360 356 314 273 245 123
85
NaeI GCC'GGC
Cuts at: 3622 3622
Size: 5441
NarI GG'CG_CC
Cuts at: 188 2872 3120 188
Size: 2684 248 2509
Fragments arranged by size:
2684 2509 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5441
NgoMIV G'CCGG_C
Cuts at: 3620 3620
Size: 5441
NspI r_CATG'y
Cuts at: 2494 2994 3901 2494
Size: 500 907 4034
Fragments arranged by size:
4034 907 500
PasI CC'CwG_GG
Cuts at: 312 312
Size: 5441
PciI A'CATG_T
Cuts at: 3897 3897
Size: 5441
PfoI T'CCnGG_A
Cuts at: 1262 1262
Size: 5441
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5441
PmlI CAC'GTG
Cuts at: 2085 2085
Size: 5441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1675 1707 2386 2418 4580 4612 1675
Size: 32 679 32 2162 32 2504
Fragments arranged by size:
2504 2162 679 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 200 1590 2336 200
Size: 1390 746 3305
Fragments arranged by size:
3305 1390 746
PspOMI G'GGCC_C
Cuts at: 2295 2295
Size: 5441
PstI C_TGCA'G
Cuts at: 1115 1805 2488 3173 1115
Size: 690 683 685 3383
Fragments arranged by size:
3383 690 685 683
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5441
PvuII CAG'CTG
Cuts at: 1923 2866 3226 1923
Size: 943 360 4138
Fragments arranged by size:
4138 943 360
RsrII CG'GwC_CG
Cuts at: 3636 3636
Size: 5441
SacI G_AGCT'C
Cuts at: 2455 2455
Size: 5441
SacII CC_GC'GG
Cuts at: 1716 1716
Size: 5441
SalI G'TCGA_C
Cuts at: 2478 2478
Size: 5441
SanDI GG'GwC_CC
Cuts at: 2336 2336
Size: 5441
SapI GCTCTTCn'nnn_
Cuts at: 2212 3464 3674 2212
Size: 1252 210 3979
Fragments arranged by size:
3979 1252 210
SbfI CC_TGCA'GG
Cuts at: 1115 2488 1115
Size: 1373 4068
ScaI AGT'ACT
Cuts at: 4958 4958
Size: 5441
SexAI A'CCwGG_T
Cuts at: 1859 2174 1859
Size: 315 5126
SfcI C'TryA_G
Cuts at: 33 1111 1801 2484 3169 4162 4353 33
Size: 1078 690 683 685 993 191 1121
Fragments arranged by size:
1121 1078 993 690 685 683 191
SfoI GGC'GCC
Cuts at: 189 2873 3121 189
Size: 2684 248 2509
Fragments arranged by size:
2684 2509 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
SmaI CCC'GGG
Cuts at: 1840 1944 2463 1840
Size: 104 519 4818
Fragments arranged by size:
4818 519 104
SmlI C'TyrA_G
Cuts at: 337 1927 2596 3511 4003 4265 337
Size: 1590 669 915 492 262 1513
Fragments arranged by size:
1590 1513 915 669 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5441
SphI G_CATG'C
Cuts at: 2494 2994 2494
Size: 500 4941
SrfI GCCC'GGGC
Cuts at: 1944 1944
Size: 5441
SspI AAT'ATT
Cuts at: 455 455
Size: 5441
StyI C'CwwG_G
Cuts at: 107 1313 1393 1868 2575 2929 107
Size: 1206 80 475 707 354 2619
Fragments arranged by size:
2619 1206 707 475 354 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1477 3024 3157 3616 3825 1477
Size: 1547 133 459 209 3093
Fragments arranged by size:
3093 1547 459 209 133
TatI w'GTAC_w
Cuts at: 4956 4956
Size: 5441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4958 4990 4958
Size: 32 5409
Tth111I GACn'n_nGTC
Cuts at: 1152 2340 3238 1152
Size: 1188 898 3355
Fragments arranged by size:
3355 1188 898
XbaI T'CTAG_A
Cuts at: 2472 2472
Size: 5441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 2936 2022
Size: 914 4527
XmaI C'CCGG_G
Cuts at: 1838 1942 2461 1838
Size: 104 519 4818
Fragments arranged by size:
4818 519 104
XmnI GAAnn'nnTTC
Cuts at: 455 521 1271 455
Size: 66 750 4625
Fragments arranged by size:
4625 750 66
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BpmI BsiWI BsmI
BsrGI BssHII BstZ17I ClaI CspCI DraIII FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI
StuI SwaI XhoI ZraI