Restriction map for
pF1KA1619
(Circular) (Six-base) MAPSORT of: pF1KA1619.seq Check: 6426 from: 1 to: 5642
pF1KA1619 5642 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2680 2680
Size: 5642
Acc65I G'GTAC_C
Cuts at: 2658 2658
Size: 5642
AclI AA'CG_TT
Cuts at: 5482 5482
Size: 5642
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 3467 3899 4252 4914 1849
Size: 1618 432 353 662 2577
Fragments arranged by size:
2577 1618 662 432 353
AflIII A'CryG_T
Cuts at: 1348 2994 4794 5075 1348
Size: 1646 1800 281 1915
Fragments arranged by size:
1915 1800 1646 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2568 2568
Size: 5642
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 748 780 748
Size: 32 5610
AlwNI CAG_nnn'CTG
Cuts at: 154 2947 4385 154
Size: 2793 1438 1411
Fragments arranged by size:
2793 1438 1411
ApaI G_GGCC'C
Cuts at: 2552 2552
Size: 5642
ApaLI G'TGCA_C
Cuts at: 4480 4480
Size: 5642
ApoI r'AATT_y
Cuts at: 667 2646 4995 5251 667
Size: 1979 2349 256 1058
Fragments arranged by size:
2349 1979 1058 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5642
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5642
AvaI C'yCGr_G
Cuts at: 323 1667 1895 2662 323
Size: 1344 228 767 3303
Fragments arranged by size:
3303 1344 767 228
BamHI G'GATC_C
Cuts at: 1469 2667 1469
Size: 1198 4444
BanI G'GyrC_C
Cuts at: 328 1212 1696 2130 2243 2658 3072 3320 3355
Size: 884 484 434 113 415 414 248 35
Cuts at: 3355 328
Size: 2615
Fragments arranged by size:
2615 884 484 434 415 414 248 113
35
BanII G_rGCy'C
Cuts at: 236 324 372 859 1223 1495 1596 2409 2552
Size: 88 48 487 364 272 101 813 143
Cuts at: 2552 2632 2656 3686 236
Size: 80 24 1030 2192
Fragments arranged by size:
2192 1030 813 487 364 272 143 101
88 80 48 24
BbeI G_GCGC'C
Cuts at: 2247 3076 3324 2247
Size: 829 248 4565
Fragments arranged by size:
4565 829 248
BbsI GAAGACnn'nnnn_
Cuts at: 1099 1837 1099
Size: 738 4904
BbvCI CC'TCA_GC
Cuts at: 136 300 548 1107 136
Size: 164 248 559 4671
Fragments arranged by size:
4671 559 248 164
BciVI GTATCCnnnnn_n'
Cuts at: 1800 3540 4596 5642 1800
Size: 1740 1056 1046 1800
Fragments arranged by size:
1800 1740 1056 1046
BclI T'GATC_A
Cuts at: 3162 3162
Size: 5642
BfrBI ATG'CAT
Cuts at: 502 738 502
Size: 236 5406
BglI GCCn_nnn'nGGC
Cuts at: 904 2278 2372 2481 904
Size: 1374 94 109 4065
Fragments arranged by size:
4065 1374 109 94
BglII A'GATC_T
Cuts at: 3157 5194 3157
Size: 2037 3605
BlpI GC'TnA_GC
Cuts at: 1078 2447 2754 1078
Size: 1369 307 3966
Fragments arranged by size:
3966 1369 307
Bme1580I G_kGCm'C
Cuts at: 333 1346 1456 2124 2552 3267 3360 4484 333
Size: 1013 110 668 428 715 93 1124 1491
Fragments arranged by size:
1491 1124 1013 715 668 428 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 396 1533 2272 2854 2935 3270 5414 396
Size: 1137 739 582 81 335 2144 624
Fragments arranged by size:
2144 1137 739 624 582 335 81
BmtI G_CTAG'C
Cuts at: 2161 2161
Size: 5642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 315 873 4009 315
Size: 558 3136 1948
Fragments arranged by size:
3136 1948 558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1120 1608 2348 2368 2389 310
Size: 810 488 740 20 21 3563
Fragments arranged by size:
3563 810 740 488 21 20
Bpu10I CC'TnA_GC
Cuts at: 136 300 548 1107 1217 1930 1994 136
Size: 164 248 559 110 713 64 3784
Fragments arranged by size:
3784 713 559 248 164 110 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1354 2818 3697 4411 4709 1029
Size: 325 1464 879 714 298 1962
Fragments arranged by size:
1962 1464 879 714 325 298
BsaI GGTCTCn'nnnn_
Cuts at: 174 917 1664 5346 174
Size: 743 747 3682 470
Fragments arranged by size:
3682 747 743 470
BsaAI yAC'GTr
Cuts at: 1351 2995 1351
Size: 1644 3998
BsaBI GATnn'nnATC
Cuts at: 6 3156 5193 6
Size: 3150 2037 455
Fragments arranged by size:
3150 2037 455
BsaHI Gr'CG_yC
Cuts at: 2244 3073 3321 4023 5519 2244
Size: 829 248 702 1496 2367
Fragments arranged by size:
2367 1496 829 702 248
BsaWI w'CCGG_w
Cuts at: 671 2831 3352 4030 4441 4588 4862 671
Size: 2160 521 678 411 147 274 1451
Fragments arranged by size:
2160 1451 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 694 724 748 778 1525 1555 694
Size: 30 24 30 747 30 4781
Fragments arranged by size:
4781 747 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 121 124 307 592 739 865 2190 2197 121
Size: 3 183 285 147 126 1325 7 3566
Fragments arranged by size:
3566 1325 285 183 147 126 7 3
BseYI C'CCAG_C
Cuts at: 606 1190 1685 2091 2359 2409 2476 3067 3825
Size: 584 495 406 268 50 67 591 758
Cuts at: 3825 4490 4898 606
Size: 665 408 1350
Fragments arranged by size:
1350 758 665 591 584 495 408 406
268 67 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 818 554
Size: 264 5378
BsiEI CG_ry'CG
Cuts at: 104 3184 3230 4460 104
Size: 3080 46 1230 1286
Fragments arranged by size:
3080 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 236 1084 1847 2000 2632 2656 3434 3624 4484
Size: 848 763 153 632 24 778 190 860
Cuts at: 4484 236
Size: 1394
Fragments arranged by size:
1394 860 848 778 763 632 190 153
24
BsmI GAATG_Cn'
Cuts at: 677 677
Size: 5642
BsmBI CGTCTCn'nnnn_
Cuts at: 985 985
Size: 5642
Bsp1286I G_dGCh'C
Cuts at: 236 324 333 372 859 1084 1223 1346 1456
Size: 88 9 39 487 225 139 123 110
Cuts at: 1456 1495 1596 1847 2000 2124 2409 2552 2632
Size: 39 101 251 153 124 285 143 80
Cuts at: 2632 2656 3267 3360 3434 3624 3686 4484 236
Size: 24 611 93 74 190 62 798 1394
Fragments arranged by size:
1394 798 611 487 285 251 225 190
153 143 139 124 123 110 101 93
88 80 74 62 39 39 24 9
BspEI T'CCGG_A
Cuts at: 671 671
Size: 5642
BspHI T'CATG_A
Cuts at: 4074 4074
Size: 5642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1448 2692 2906 3208 3589 1448
Size: 1244 214 302 381 3501
Fragments arranged by size:
3501 1244 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1838 3934 3988 5472 5637 50
Size: 1788 2096 54 1484 165 55
Fragments arranged by size:
2096 1788 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1249 2162 3554 1249
Size: 913 1392 3337
Fragments arranged by size:
3337 1392 913
BsrFI r'CCGG_y
Cuts at: 2442 2480 3640 3821 2442
Size: 38 1160 181 4263
Fragments arranged by size:
4263 1160 181 38
BssSI C'ACGA_G
Cuts at: 775 908 1233 2608 3913 4621 775
Size: 133 325 1375 1305 708 1796
Fragments arranged by size:
1796 1375 1305 708 325 133
BstAPI GCAn_nnn'nTGC
Cuts at: 3019 3019
Size: 5642
BstBI TT'CG_AA
Cuts at: 4003 4003
Size: 5642
BstXI CCAn_nnnn'nTGG
Cuts at: 740 801 2326 2463 740
Size: 61 1525 137 3919
Fragments arranged by size:
3919 1525 137 61
BstYI r'GATC_y
Cuts at: 1 380 941 1136 1469 2287 2667 3134 3157
Size: 379 561 195 333 818 380 467 23
Cuts at: 3157 3492 3738 4044 4056 4142 5194 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 818 561 467 449 380 379 335
333 306 246 195 86 23 12
BtgI C'CryG_G
Cuts at: 612 612
Size: 5642
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1949 3592 3773 3936 1949
Size: 1643 181 163 3655
Fragments arranged by size:
3655 1643 181 163
BtsI GCAGTG_nn'
Cuts at: 172 280 806 1009 2470 2885 172
Size: 108 526 203 1461 415 2929
Fragments arranged by size:
2929 1461 526 415 203 108
DraI TTT'AAA
Cuts at: 2641 2641
Size: 5642
DraIII CAC_nnn'GTG
Cuts at: 894 1015 1088 894
Size: 121 73 5448
Fragments arranged by size:
5448 121 73
DrdI GACnn_nn'nnGTC
Cuts at: 3348 4692 3348
Size: 1344 4298
EaeI y'GGCC_r
Cuts at: 2074 3227 3401 3792 3819 5164 2074
Size: 1153 174 391 27 1345 2552
Fragments arranged by size:
2552 1345 1153 391 174 27
EagI C'GGCC_G
Cuts at: 3227 3227
Size: 5642
EarI CTCTTCn'nnn_
Cuts at: 224 1508 3665 3875 224
Size: 1284 2157 210 1991
Fragments arranged by size:
2157 1991 1284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 679 3850 4592 4738 5452 679
Size: 3171 742 146 714 869
Fragments arranged by size:
3171 869 742 714 146
EcoICRI GAG'CTC
Cuts at: 234 2630 2654 234
Size: 2396 24 3222
Fragments arranged by size:
3222 2396 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1120 1608 1849 2348 2368 2389 3467 3899
Size: 810 488 241 499 20 21 1078 432
Cuts at: 3899 4252 4914 310
Size: 353 662 1038
Fragments arranged by size:
1078 1038 810 662 499 488 432 353
241 21 20
EcoNI CCTnn'n_nnAGG
Cuts at: 303 303
Size: 5642
EcoO109I rG'GnC_Cy
Cuts at: 166 242 1156 1689 1736 2364 2548 2549 2781
Size: 76 914 533 47 628 184 1 232
Cuts at: 2781 166
Size: 3027
Fragments arranged by size:
3027 914 628 533 232 184 76 47
1
EcoRI G'AATT_C
Cuts at: 667 2646 667
Size: 1979 3663
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 5642
FspI TGC'GCA
Cuts at: 1255 1710 3423 1255
Size: 455 1713 3474
Fragments arranged by size:
3474 1713 455
HaeII r_GCGC'y
Cuts at: 1191 2247 3076 3324 4554 5325 1191
Size: 1056 829 248 1230 771 1508
Fragments arranged by size:
1508 1230 1056 829 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1273 1305 1525 1557 1767 1799 1867 1899 2614
Size: 32 220 32 210 32 68 32 715
Cuts at: 2614 2646 3484 3514 3516 3546 1273
Size: 32 838 30 2 30 3369
Fragments arranged by size:
3369 838 715 220 210 68 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2681 2681
Size: 5642
KasI G'GCGC_C
Cuts at: 2243 3072 3320 2243
Size: 829 248 4565
Fragments arranged by size:
4565 829 248
KpnI G_GTAC'C
Cuts at: 2662 2662
Size: 5642
MluI A'CGCG_T
Cuts at: 5075 5075
Size: 5642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1910 4401 4585 1910
Size: 2491 184 2967
Fragments arranged by size:
2967 2491 184
MscI TGG'CCA
Cuts at: 3403 3403
Size: 5642
MslI CAynn'nnrTG
Cuts at: 497 1061 3631 3758 497
Size: 564 2570 127 2381
Fragments arranged by size:
2570 2381 564 127
MspA1I CmG'CkG
Cuts at: 477 1459 1628 2127 2378 2753 3067 3427 4211
Size: 982 169 499 251 375 314 360 784
Cuts at: 4211 4456 477
Size: 245 1663
Fragments arranged by size:
1663 982 784 499 375 360 314 251
245 169
NaeI GCC'GGC
Cuts at: 2482 3823 2482
Size: 1341 4301
NarI GG'CG_CC
Cuts at: 2244 3073 3321 2244
Size: 829 248 4565
Fragments arranged by size:
4565 829 248
NgoMIV G'CCGG_C
Cuts at: 2480 3821 2480
Size: 1341 4301
NheI G'CTAG_C
Cuts at: 2157 2157
Size: 5642
NsiI A_TGCA'T
Cuts at: 504 740 504
Size: 236 5406
NspI r_CATG'y
Cuts at: 506 2695 3195 4798 506
Size: 2189 500 1603 1350
Fragments arranged by size:
2189 1603 1350 500
PasI CC'CwG_GG
Cuts at: 1740 2395 1740
Size: 655 4987
PciI A'CATG_T
Cuts at: 4794 4794
Size: 5642
PflMI CCAn_nnn'nTGG
Cuts at: 613 1545 613
Size: 932 4710
PfoI T'CCnGG_A
Cuts at: 1298 2325 2582 1298
Size: 1027 257 4358
Fragments arranged by size:
4358 1027 257
PmeI GTTT'AAAC
Cuts at: 2641 2641
Size: 5642
PmlI CAC'GTG
Cuts at: 1351 1351
Size: 5642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 748 780 4088 4120 748
Size: 32 3308 32 2270
Fragments arranged by size:
3308 2270 32 32
PpuMI rG'GwC_Cy
Cuts at: 166 242 1156 1689 1736 166
Size: 76 914 533 47 4072
Fragments arranged by size:
4072 914 533 76 47
PshAI GACnn'nnGTC
Cuts at: 1408 1408
Size: 5642
PspOMI G'GGCC_C
Cuts at: 2548 2548
Size: 5642
PstI C_TGCA'G
Cuts at: 159 476 950 1027 2306 2689 3374 159
Size: 317 474 77 1279 383 685 2427
Fragments arranged by size:
2427 1279 685 474 383 317 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5642
PvuII CAG'CTG
Cuts at: 1459 1628 2127 2378 3067 3427 1459
Size: 169 499 251 689 360 3674
Fragments arranged by size:
3674 689 499 360 251 169
RsrII CG'GwC_CG
Cuts at: 3837 3837
Size: 5642
SacI G_AGCT'C
Cuts at: 236 2632 2656 236
Size: 2396 24 3222
Fragments arranged by size:
3222 2396 24
SalI G'TCGA_C
Cuts at: 2679 2679
Size: 5642
SanDI GG'GwC_CC
Cuts at: 242 1689 242
Size: 1447 4195
SapI GCTCTTCn'nnn_
Cuts at: 224 3665 3875 224
Size: 3441 210 1991
Fragments arranged by size:
3441 1991 210
SbfI CC_TGCA'GG
Cuts at: 2689 2689
Size: 5642
ScaI AGT'ACT
Cuts at: 5159 5159
Size: 5642
SfcI C'TryA_G
Cuts at: 33 155 472 946 1023 2058 2302 2685 3370
Size: 122 317 474 77 1035 244 383 685
Cuts at: 3370 4338 4529 33
Size: 968 191 1146
Fragments arranged by size:
1146 1035 968 685 474 383 317 244
191 122 77
SfoI GGC'GCC
Cuts at: 2245 3074 3322 2245
Size: 829 248 4565
Fragments arranged by size:
4565 829 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5642
SmaI CCC'GGG
Cuts at: 1897 2664 1897
Size: 767 4875
SmlI C'TyrA_G
Cuts at: 1044 1369 2797 3712 4426 4688 1044
Size: 325 1428 915 714 262 1998
Fragments arranged by size:
1998 1428 915 714 325 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5642
SphI G_CATG'C
Cuts at: 2695 3195 2695
Size: 500 5142
SrfI GCCC'GGGC
Cuts at: 1897 1897
Size: 5642
StuI AGG'CCT
Cuts at: 298 553 626 644 1105 298
Size: 255 73 18 461 4835
Fragments arranged by size:
4835 461 255 73 18
StyI C'CwwG_G
Cuts at: 1260 2776 3130 1260
Size: 1516 354 3772
Fragments arranged by size:
3772 1516 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 719 793 1334 1714 3225 3358 3817 4026 719
Size: 74 541 380 1511 133 459 209 2335
Fragments arranged by size:
2335 1511 541 459 380 209 133 74
TatI w'GTAC_w
Cuts at: 821 5157 821
Size: 4336 1306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 694 726 2279 2311 5159 5191 694
Size: 32 1553 32 2848 32 1145
Fragments arranged by size:
2848 1553 1145 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3439 3439
Size: 5642
XbaI T'CTAG_A
Cuts at: 187 2673 187
Size: 2486 3156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2392 2463 3137 2392
Size: 71 674 4897
Fragments arranged by size:
4897 674 71
XmaI C'CCGG_G
Cuts at: 1895 2662 1895
Size: 767 4875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AscI
AvrII BaeI BcgI BmgBI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI PacI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI