Restriction map for
pF1KA1604
(Circular) (Six-base) MAPSORT of: pF1KA1604.seq Check: 9122 from: 1 to: 5837
pF1KA1604 5837 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2875 2875
Size: 5837
Acc65I G'GTAC_C
Cuts at: 2853 2853
Size: 5837
AclI AA'CG_TT
Cuts at: 5677 5677
Size: 5837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 2108 2123 3662 4094 4841 5109 613
Size: 1495 15 1539 432 747 268 1341
Fragments arranged by size:
1539 1495 1341 747 432 268 15
AflII C'TTAA_G
Cuts at: 997 997
Size: 5837
AflIII A'CryG_T
Cuts at: 3189 4293 5270 3189
Size: 1104 977 3756
Fragments arranged by size:
3756 1104 977
AgeI A'CCGG_T
Cuts at: 4225 4225
Size: 5837
AlwNI CAG_nnn'CTG
Cuts at: 1875 2146 3142 4709 1875
Size: 271 996 1567 3003
Fragments arranged by size:
3003 1567 996 271
ApaLI G'TGCA_C
Cuts at: 4607 4607
Size: 5837
ApoI r'AATT_y
Cuts at: 781 880 1319 1621 1847 2418 2589 2640 2841
Size: 99 439 302 226 571 171 51 201
Cuts at: 2841 5190 5446 781
Size: 2349 256 1172
Fragments arranged by size:
2349 1172 571 439 302 256 226 201
171 99 51
AseI AT'TA_AT
Cuts at: 20 605 802 20
Size: 585 197 5055
Fragments arranged by size:
5055 585 197
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5837
AvaI C'yCGr_G
Cuts at: 227 1958 2857 227
Size: 1731 899 3207
Fragments arranged by size:
3207 1731 899
BamHI G'GATC_C
Cuts at: 474 1269 2862 474
Size: 795 1593 3449
Fragments arranged by size:
3449 1593 795
BanI G'GyrC_C
Cuts at: 2363 2728 2853 3267 3515 3550 2363
Size: 365 125 414 248 35 4650
Fragments arranged by size:
4650 414 365 248 125 35
BanII G_rGCy'C
Cuts at: 2851 3881 2851
Size: 1030 4807
BbeI G_GCGC'C
Cuts at: 3271 3519 3271
Size: 248 5589
BbsI GAAGACnn'nnnn_
Cuts at: 203 1885 2186 2284 2355 203
Size: 1682 301 98 71 3685
Fragments arranged by size:
3685 1682 301 98 71
BbvCI CC'TCA_GC
Cuts at: 2244 2244
Size: 5837
BciVI GTATCCnnnnn_n'
Cuts at: 3735 4496 5837 3735
Size: 761 1341 3735
Fragments arranged by size:
3735 1341 761
BclI T'GATC_A
Cuts at: 1197 2306 3357 1197
Size: 1109 1051 3677
Fragments arranged by size:
3677 1109 1051
BfrBI ATG'CAT
Cuts at: 2532 2532
Size: 5837
BglII A'GATC_T
Cuts at: 3352 5389 3352
Size: 2037 3800
BlpI GC'TnA_GC
Cuts at: 561 2949 561
Size: 2388 3449
Bme1580I G_kGCm'C
Cuts at: 1602 2366 3462 3555 4611 1602
Size: 764 1096 93 1056 2828
Fragments arranged by size:
2828 1096 1056 764 93
BmrI ACTGGGnnnn_n'
Cuts at: 3049 3130 3465 5609 3049
Size: 81 335 2144 3277
Fragments arranged by size:
3277 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 746 2166 2229 4204 746
Size: 1420 63 1975 2379
Fragments arranged by size:
2379 1975 1420 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 1243 2801 176
Size: 1067 1558 3212
Fragments arranged by size:
3212 1558 1067
Bpu10I CC'TnA_GC
Cuts at: 434 523 2244 434
Size: 89 1721 4027
Fragments arranged by size:
4027 1721 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2682 3013 3892 4384 4682 2682
Size: 331 879 492 298 3837
Fragments arranged by size:
3837 879 492 331 298
BsaI GGTCTCn'nnnn_
Cuts at: 374 1470 2513 5541 374
Size: 1096 1043 3028 670
Fragments arranged by size:
3028 1096 1043 670
BsaAI yAC'GTr
Cuts at: 3190 3190
Size: 5837
BsaBI GATnn'nnATC
Cuts at: 6 356 2717 3351 5388 6
Size: 350 2361 634 2037 455
Fragments arranged by size:
2361 2037 634 455 350
BsaHI Gr'CG_yC
Cuts at: 3268 3516 4218 5714 3268
Size: 248 702 1496 3391
Fragments arranged by size:
3391 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3026 3547 4225 4499 4646 5057 3026
Size: 521 678 274 147 411 3806
Fragments arranged by size:
3806 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 408 2124 2591 177
Size: 231 1716 467 3423
Fragments arranged by size:
3423 1716 467 231
BseYI C'CCAG_C
Cuts at: 1363 1639 3262 4020 4597 5093 1363
Size: 276 1623 758 577 496 2107
Fragments arranged by size:
2107 1623 758 577 496 276
BsiEI CG_ry'CG
Cuts at: 104 3379 3425 4633 104
Size: 3275 46 1208 1308
Fragments arranged by size:
3275 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1527 2851 3629 3819 4611 1527
Size: 1324 778 190 792 2753
Fragments arranged by size:
2753 1324 792 778 190
BsmI GAATG_Cn'
Cuts at: 270 270
Size: 5837
BsmBI CGTCTCn'nnnn_
Cuts at: 2730 2730
Size: 5837
Bsp1286I G_dGCh'C
Cuts at: 1527 1602 2366 2851 3462 3555 3629 3819 3881
Size: 75 764 485 611 93 74 190 62
Cuts at: 3881 4611 1527
Size: 730 2753
Fragments arranged by size:
2753 764 730 611 485 190 93 75
74 62
BspEI T'CCGG_A
Cuts at: 5057 5057
Size: 5837
BspHI T'CATG_A
Cuts at: 147 1440 5013 147
Size: 1293 3573 971
Fragments arranged by size:
3573 1293 971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 453 2887 3101 3403 3784 453
Size: 2434 214 302 381 2506
Fragments arranged by size:
2506 2434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4129 4183 5667 5832 50
Size: 4079 54 1484 165 55
Fragments arranged by size:
4079 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3749 3749
Size: 5837
BsrFI r'CCGG_y
Cuts at: 2798 3835 4016 4225 2798
Size: 1037 181 209 4410
Fragments arranged by size:
4410 1037 209 181
BssSI C'ACGA_G
Cuts at: 4108 4466 4108
Size: 358 5479
BstAPI GCAn_nnn'nTGC
Cuts at: 724 3214 724
Size: 2490 3347
BstBI TT'CG_AA
Cuts at: 831 1065 4198 831
Size: 234 3133 2470
Fragments arranged by size:
3133 2470 234
BstXI CCAn_nnnn'nTGG
Cuts at: 1363 1363
Size: 5837
BstYI r'GATC_y
Cuts at: 1 474 1269 2442 2862 3329 3352 3687 3933
Size: 473 795 1173 420 467 23 335 246
Cuts at: 3933 4945 5031 5043 5389 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1173 1012 795 473 467 449 420 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 1789 1789
Size: 5837
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1702 3787 3968 4131 1702
Size: 2085 181 163 3408
Fragments arranged by size:
3408 2085 181 163
BtsI GCAGTG_nn'
Cuts at: 2181 3080 2181
Size: 899 4938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 866 901 866
Size: 35 5802
DraI TTT'AAA
Cuts at: 2836 2836
Size: 5837
DraIII CAC_nnn'GTG
Cuts at: 724 724
Size: 5837
DrdI GACnn_nn'nnGTC
Cuts at: 3543 4401 3543
Size: 858 4979
EaeI y'GGCC_r
Cuts at: 3422 3596 3987 4014 5359 3422
Size: 174 391 27 1345 3900
Fragments arranged by size:
3900 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3422 3422
Size: 5837
EarI CTCTTCn'nnn_
Cuts at: 273 485 681 1286 1370 1388 1394 2121 2127
Size: 212 196 605 84 18 6 727 6
Cuts at: 2127 2142 2198 2220 2566 2799 3860 4070 273
Size: 15 56 22 346 233 1061 210 2040
Fragments arranged by size:
2040 1061 727 605 346 233 212 210
196 84 56 22 18 15 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4045 4355 4501 5647 4045
Size: 310 146 1146 4235
Fragments arranged by size:
4235 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2849 2849
Size: 5837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 613 1243 2108 2123 2801 3662 4094 4841
Size: 437 630 865 15 678 861 432 747
Cuts at: 4841 5109 176
Size: 268 904
Fragments arranged by size:
904 865 861 747 678 630 437 432
268 15
EcoO109I rG'GnC_Cy
Cuts at: 588 2976 588
Size: 2388 3449
EcoRI G'AATT_C
Cuts at: 2841 2841
Size: 5837
EcoRV GAT'ATC
Cuts at: 2719 2719
Size: 5837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 679 1267 1339 2609 3856 679
Size: 588 72 1270 1247 2660
Fragments arranged by size:
2660 1270 1247 588 72
FspI TGC'GCA
Cuts at: 3618 3618
Size: 5837
HaeII r_GCGC'y
Cuts at: 3271 3519 4541 5520 3271
Size: 248 1022 979 3588
Fragments arranged by size:
3588 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 788 820 1327 1359 1746 1778 2203 2235 3679
Size: 32 507 32 387 32 425 32 1444
Cuts at: 3679 3709 3711 3741 788
Size: 30 2 30 2884
Fragments arranged by size:
2884 1444 507 425 387 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 627 2876 627
Size: 2249 3588
KasI G'GCGC_C
Cuts at: 3267 3515 3267
Size: 248 5589
KpnI G_GTAC'C
Cuts at: 2857 2857
Size: 5837
MluI A'CGCG_T
Cuts at: 5270 5270
Size: 5837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 2180 4508 4692 659
Size: 1521 2328 184 1804
Fragments arranged by size:
2328 1804 1521 184
MscI TGG'CCA
Cuts at: 3598 3598
Size: 5837
MslI CAynn'nnrTG
Cuts at: 749 1210 1788 3826 3953 749
Size: 461 578 2038 127 2633
Fragments arranged by size:
2633 2038 578 461 127
MspA1I CmG'CkG
Cuts at: 2948 3262 3622 4635 4880 2948
Size: 314 360 1013 245 3905
Fragments arranged by size:
3905 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4018 4018
Size: 5837
NarI GG'CG_CC
Cuts at: 3268 3516 3268
Size: 248 5589
NcoI C'CATG_G
Cuts at: 1789 1789
Size: 5837
NdeI CA'TA_TG
Cuts at: 1211 1211
Size: 5837
NgoMIV G'CCGG_C
Cuts at: 4016 4016
Size: 5837
NsiI A_TGCA'T
Cuts at: 2534 2534
Size: 5837
NspI r_CATG'y
Cuts at: 1656 2890 3390 4297 1656
Size: 1234 500 907 3196
Fragments arranged by size:
3196 1234 907 500
PciI A'CATG_T
Cuts at: 4293 4293
Size: 5837
PflMI CCAn_nnn'nTGG
Cuts at: 388 793 909 1722 388
Size: 405 116 813 4503
Fragments arranged by size:
4503 813 405 116
PfoI T'CCnGG_A
Cuts at: 950 1868 950
Size: 918 4919
PmeI GTTT'AAAC
Cuts at: 2836 2836
Size: 5837
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4976 5008 4976
Size: 32 5805
PshAI GACnn'nnGTC
Cuts at: 696 696
Size: 5837
PstI C_TGCA'G
Cuts at: 1934 2884 3569 1934
Size: 950 685 4202
Fragments arranged by size:
4202 950 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5837
PvuII CAG'CTG
Cuts at: 3262 3622 3262
Size: 360 5477
RsrII CG'GwC_CG
Cuts at: 4032 4032
Size: 5837
SacI G_AGCT'C
Cuts at: 2851 2851
Size: 5837
SalI G'TCGA_C
Cuts at: 2874 2874
Size: 5837
SapI GCTCTTCn'nnn_
Cuts at: 3860 4070 3860
Size: 210 5627
SbfI CC_TGCA'GG
Cuts at: 2884 2884
Size: 5837
ScaI AGT'ACT
Cuts at: 5354 5354
Size: 5837
SexAI A'CCwGG_T
Cuts at: 1460 1460
Size: 5837
SfcI C'TryA_G
Cuts at: 33 1930 2006 2496 2880 3565 4558 4749 33
Size: 1897 76 490 384 685 993 191 1121
Fragments arranged by size:
1897 1121 993 685 490 384 191 76
SfoI GGC'GCC
Cuts at: 3269 3517 3269
Size: 248 5589
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5837
SmaI CCC'GGG
Cuts at: 229 2859 229
Size: 2630 3207
SmlI C'TyrA_G
Cuts at: 997 2697 2992 3907 4399 4661 997
Size: 1700 295 915 492 262 2173
Fragments arranged by size:
2173 1700 915 492 295 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5837
SphI G_CATG'C
Cuts at: 1656 2890 3390 1656
Size: 1234 500 4103
Fragments arranged by size:
4103 1234 500
SspI AAT'ATT
Cuts at: 1862 1862
Size: 5837
StyI C'CwwG_G
Cuts at: 1789 2971 3325 1789
Size: 1182 354 4301
Fragments arranged by size:
4301 1182 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 338 757 3420 3553 4012 4221 338
Size: 419 2663 133 459 209 1954
Fragments arranged by size:
2663 1954 459 419 209 133
TatI w'GTAC_w
Cuts at: 1298 1667 2456 5352 1298
Size: 369 789 2896 1783
Fragments arranged by size:
2896 1783 789 369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5354 5386 5354
Size: 32 5805
Tth111I GACn'n_nGTC
Cuts at: 3634 3634
Size: 5837
XbaI T'CTAG_A
Cuts at: 2868 2868
Size: 5837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1719 3332 1719
Size: 1613 4224
XmaI C'CCGG_G
Cuts at: 227 2857 227
Size: 2630 3207
XmnI GAAnn'nnTTC
Cuts at: 1061 1247 2589 1061
Size: 186 1342 4309
Fragments arranged by size:
4309 1342 186
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
EcoNI FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PasI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI