Restriction map for
pF1KA1598
(Circular) (Six-base) MAPSORT of: pF1KA1598.seq Check: 3498 from: 1 to: 5006
pF1KA1598 5006 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2044 2044
Size: 5006
Acc65I G'GTAC_C
Cuts at: 2022 2022
Size: 5006
AclI AA'CG_TT
Cuts at: 394 4846 394
Size: 4452 554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 377 470 1109 1325 2831 3263 3616 4278
Size: 202 93 639 216 1506 432 353 662
Cuts at: 4278 175
Size: 903
Fragments arranged by size:
1506 903 662 639 432 353 216 202
93
AflIII A'CryG_T
Cuts at: 2358 4158 4439 2358
Size: 1800 281 2925
Fragments arranged by size:
2925 1800 281
AleI CACnn'nnGTG
Cuts at: 1256 1256
Size: 5006
AlwNI CAG_nnn'CTG
Cuts at: 1035 2311 3749 1035
Size: 1276 1438 2292
Fragments arranged by size:
2292 1438 1276
ApaI G_GGCC'C
Cuts at: 1739 1739
Size: 5006
ApaLI G'TGCA_C
Cuts at: 3844 3844
Size: 5006
ApoI r'AATT_y
Cuts at: 268 278 675 947 1083 1104 1155 1693 1937
Size: 10 397 272 136 21 51 538 244
Cuts at: 1937 2010 4359 4615 268
Size: 73 2349 256 659
Fragments arranged by size:
2349 659 538 397 272 256 244 136
73 51 21 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 2026 2026
Size: 5006
BamHI G'GATC_C
Cuts at: 2031 2031
Size: 5006
BanI G'GyrC_C
Cuts at: 496 2022 2436 2684 2719 496
Size: 1526 414 248 35 2783
Fragments arranged by size:
2783 1526 414 248 35
BanII G_rGCy'C
Cuts at: 874 1739 2020 3050 874
Size: 865 281 1030 2830
Fragments arranged by size:
2830 1030 865 281
BbeI G_GCGC'C
Cuts at: 2440 2688 2440
Size: 248 4758
BbsI GAAGACnn'nnnn_
Cuts at: 188 343 1411 1964 188
Size: 155 1068 553 3230
Fragments arranged by size:
3230 1068 553 155
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2904 3960 5006 1656
Size: 1248 1056 1046 1656
Fragments arranged by size:
1656 1248 1056 1046
BclI T'GATC_A
Cuts at: 882 2526 882
Size: 1644 3362
BglII A'GATC_T
Cuts at: 1317 2521 4558 1317
Size: 1204 2037 1765
Fragments arranged by size:
2037 1765 1204
BlpI GC'TnA_GC
Cuts at: 2118 2118
Size: 5006
Bme1580I G_kGCm'C
Cuts at: 1739 2631 2724 3848 1739
Size: 892 93 1124 2897
Fragments arranged by size:
2897 1124 892 93
BmrI ACTGGGnnnn_n'
Cuts at: 1250 1474 1604 1781 2218 2299 2634 4778 1250
Size: 224 130 177 437 81 335 2144 1478
Fragments arranged by size:
2144 1478 437 335 224 177 130 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 5006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 796 1057 1114 1705 790
Size: 6 261 57 591 4091
Fragments arranged by size:
4091 591 261 57 6
Bpu10I CC'TnA_GC
Cuts at: 1721 1721
Size: 5006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 2182 3061 3775 4073 595
Size: 1587 879 714 298 1528
Fragments arranged by size:
1587 1528 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4710 4710
Size: 5006
BsaAI yAC'GTr
Cuts at: 2359 2359
Size: 5006
BsaBI GATnn'nnATC
Cuts at: 6 2520 4557 6
Size: 2514 2037 455
Fragments arranged by size:
2514 2037 455
BsaHI Gr'CG_yC
Cuts at: 2437 2685 3387 4883 2437
Size: 248 702 1496 2560
Fragments arranged by size:
2560 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1238 2195 2716 3394 3805 3952 4226 1238
Size: 957 521 678 411 147 274 2018
Fragments arranged by size:
2018 957 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 766 1166 1169 766
Size: 400 3 4603
Fragments arranged by size:
4603 400 3
BseYI C'CCAG_C
Cuts at: 429 926 2431 3189 3854 4262 429
Size: 497 1505 758 665 408 1173
Fragments arranged by size:
1505 1173 758 665 497 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1460 1460
Size: 5006
BsiEI CG_ry'CG
Cuts at: 104 2548 2594 3824 104
Size: 2444 46 1230 1286
Fragments arranged by size:
2444 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 874 2020 2798 2988 3848 874
Size: 1146 778 190 860 2032
Fragments arranged by size:
2032 1146 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 874 1739 2020 2631 2724 2798 2988 3050 3848
Size: 865 281 611 93 74 190 62 798
Cuts at: 3848 874
Size: 2032
Fragments arranged by size:
2032 865 798 611 281 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1238 1238
Size: 5006
BspHI T'CATG_A
Cuts at: 3438 3438
Size: 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2056 2270 2572 2953 2056
Size: 214 302 381 4109
Fragments arranged by size:
4109 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3298 3352 4836 5001 50
Size: 3248 54 1484 165 55
Fragments arranged by size:
3248 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2918 2918
Size: 5006
BsrFI r'CCGG_y
Cuts at: 3004 3185 3004
Size: 181 4825
BsrGI T'GTAC_A
Cuts at: 416 416
Size: 5006
BssSI C'ACGA_G
Cuts at: 3277 3985 3277
Size: 708 4298
BstAPI GCAn_nnn'nTGC
Cuts at: 1799 2383 1799
Size: 584 4422
BstBI TT'CG_AA
Cuts at: 789 1423 3367 789
Size: 634 1944 2428
Fragments arranged by size:
2428 1944 634
BstXI CCAn_nnnn'nTGG
Cuts at: 600 1258 600
Size: 658 4348
BstYI r'GATC_y
Cuts at: 1 1317 1854 1905 2031 2498 2521 2856 3102
Size: 1316 537 51 126 467 23 335 246
Cuts at: 3102 3408 3420 3506 4558 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1316 1052 537 467 449 335 306 246
126 86 51 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2956 3137 3300 2956
Size: 181 163 4662
Fragments arranged by size:
4662 181 163
BtsI GCAGTG_nn'
Cuts at: 1269 1448 2249 1269
Size: 179 801 4026
Fragments arranged by size:
4026 801 179
DraI TTT'AAA
Cuts at: 674 1109 2005 674
Size: 435 896 3675
Fragments arranged by size:
3675 896 435
DrdI GACnn_nn'nnGTC
Cuts at: 2712 4056 2712
Size: 1344 3662
EaeI y'GGCC_r
Cuts at: 422 2591 2765 3156 3183 4528 422
Size: 2169 174 391 27 1345 900
Fragments arranged by size:
2169 1345 900 391 174 27
EagI C'GGCC_G
Cuts at: 2591 2591
Size: 5006
EarI CTCTTCn'nnn_
Cuts at: 119 446 737 935 974 1085 1334 3029 3239
Size: 327 291 198 39 111 249 1695 210
Cuts at: 3239 119
Size: 1886
Fragments arranged by size:
1886 1695 327 291 249 210 198 111
39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3214 3956 4102 4816 3214
Size: 742 146 714 3404
Fragments arranged by size:
3404 742 714 146
EcoICRI GAG'CTC
Cuts at: 872 2018 872
Size: 1146 3860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 377 470 790 796 1057 1109 1114 1325
Size: 202 93 320 6 261 52 5 211
Cuts at: 1325 1705 2831 3263 3616 4278 175
Size: 380 1126 432 353 662 903
Fragments arranged by size:
1126 903 662 432 380 353 320 261
211 202 93 52 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1724 1724
Size: 5006
EcoO109I rG'GnC_Cy
Cuts at: 783 1735 1736 2145 783
Size: 952 1 409 3644
Fragments arranged by size:
3644 952 409 1
EcoRI G'AATT_C
Cuts at: 947 1083 1155 1693 2010 947
Size: 136 72 538 317 3943
Fragments arranged by size:
3943 538 317 136 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 5006
FspI TGC'GCA
Cuts at: 2787 2787
Size: 5006
HaeII r_GCGC'y
Cuts at: 2440 2688 3918 4689 2440
Size: 248 1230 771 2757
Fragments arranged by size:
2757 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1536 1568 1606 1638 2848 2878 2880 2910 1536
Size: 32 38 32 1210 30 2 30 3632
Fragments arranged by size:
3632 1210 38 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2045 2045
Size: 5006
HindIII A'AGCT_T
Cuts at: 359 899 1497 359
Size: 540 598 3868
Fragments arranged by size:
3868 598 540
KasI G'GCGC_C
Cuts at: 2436 2684 2436
Size: 248 4758
KpnI G_GTAC'C
Cuts at: 2026 2026
Size: 5006
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1615 1933 2018 3765 3949 1615
Size: 318 85 1747 184 2672
Fragments arranged by size:
2672 1747 318 184 85
MscI TGG'CCA
Cuts at: 424 2767 424
Size: 2343 2663
MslI CAynn'nnrTG
Cuts at: 1231 1256 1630 2995 3122 1231
Size: 25 374 1365 127 3115
Fragments arranged by size:
3115 1365 374 127 25
MspA1I CmG'CkG
Cuts at: 135 2117 2431 2791 3575 3820 135
Size: 1982 314 360 784 245 1321
Fragments arranged by size:
1982 1321 784 360 314 245
NaeI GCC'GGC
Cuts at: 3187 3187
Size: 5006
NarI GG'CG_CC
Cuts at: 2437 2685 2437
Size: 248 4758
NgoMIV G'CCGG_C
Cuts at: 3185 3185
Size: 5006
NspI r_CATG'y
Cuts at: 414 2059 2559 4162 414
Size: 1645 500 1603 1258
Fragments arranged by size:
1645 1603 1258 500
PciI A'CATG_T
Cuts at: 4158 4158
Size: 5006
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4974
PpuMI rG'GwC_Cy
Cuts at: 783 783
Size: 5006
PshAI GACnn'nnGTC
Cuts at: 511 511
Size: 5006
PspOMI G'GGCC_C
Cuts at: 1735 1735
Size: 5006
PstI C_TGCA'G
Cuts at: 140 869 2053 2738 140
Size: 729 1184 685 2408
Fragments arranged by size:
2408 1184 729 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5006
PvuII CAG'CTG
Cuts at: 135 2431 2791 135
Size: 2296 360 2350
Fragments arranged by size:
2350 2296 360
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 874 2020 874
Size: 1146 3860
SalI G'TCGA_C
Cuts at: 2043 2043
Size: 5006
SapI GCTCTTCn'nnn_
Cuts at: 974 3029 3239 974
Size: 2055 210 2741
Fragments arranged by size:
2741 2055 210
SbfI CC_TGCA'GG
Cuts at: 2053 2053
Size: 5006
ScaI AGT'ACT
Cuts at: 4523 4523
Size: 5006
SexAI A'CCwGG_T
Cuts at: 1889 1889
Size: 5006
SfcI C'TryA_G
Cuts at: 33 136 482 737 865 2049 2734 3702 3893
Size: 103 346 255 128 1184 685 968 191
Cuts at: 3893 33
Size: 1146
Fragments arranged by size:
1184 1146 968 685 346 255 191 128
103
SfoI GGC'GCC
Cuts at: 2438 2686 2438
Size: 248 4758
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 2028 2028
Size: 5006
SmlI C'TyrA_G
Cuts at: 610 2161 3076 3790 4052 610
Size: 1551 915 714 262 1564
Fragments arranged by size:
1564 1551 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1486 67
Size: 1419 3587
SphI G_CATG'C
Cuts at: 2059 2559 2059
Size: 500 4506
StyI C'CwwG_G
Cuts at: 1766 2140 2494 1766
Size: 374 354 4278
Fragments arranged by size:
4278 374 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 940 1416 2589 2722 3181 3390 940
Size: 476 1173 133 459 209 2556
Fragments arranged by size:
2556 1173 476 459 209 133
TatI w'GTAC_w
Cuts at: 416 519 4521 416
Size: 103 4002 901
Fragments arranged by size:
4002 901 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4523 4555 4523
Size: 32 4974
Tth111I GACn'n_nGTC
Cuts at: 2803 2803
Size: 5006
XbaI T'CTAG_A
Cuts at: 1044 2037 1044
Size: 993 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1632 2501 1632
Size: 869 4137
XmaI C'CCGG_G
Cuts at: 2026 2026
Size: 5006
XmnI GAAnn'nnTTC
Cuts at: 269 269
Size: 5006
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI