Restriction map for
pF1KA1584
(Circular) (Six-base) MAPSORT of: pF1KA1584.seq Check: 9852 from: 1 to: 6011
pF1KA1584 6011 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1153 1153
Size: 6011
AccI GT'mk_AC
Cuts at: 2978 3049 2978
Size: 71 5940
Acc65I G'GTAC_C
Cuts at: 3027 3027
Size: 6011
AclI AA'CG_TT
Cuts at: 5851 5851
Size: 6011
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 632 674 689 1023 1613 1678 2857 2879
Size: 237 42 15 334 590 65 1179 22
Cuts at: 2879 3836 4268 5015 5283 395
Size: 957 432 747 268 1123
Fragments arranged by size:
1179 1123 957 747 590 432 334 268
237 65 42 22 15
AflII C'TTAA_G
Cuts at: 2199 2199
Size: 6011
AflIII A'CryG_T
Cuts at: 1269 2598 3363 4467 5444 1269
Size: 1329 765 1104 977 1836
Fragments arranged by size:
1836 1329 1104 977 765
AgeI A'CCGG_T
Cuts at: 4399 4399
Size: 6011
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 765 797 765
Size: 32 5979
AlwNI CAG_nnn'CTG
Cuts at: 373 3316 4883 373
Size: 2943 1567 1501
Fragments arranged by size:
2943 1567 1501
ApaLI G'TGCA_C
Cuts at: 296 4781 296
Size: 4485 1526
ApoI r'AATT_y
Cuts at: 387 669 898 1052 1222 1914 2373 2433 3015
Size: 282 229 154 170 692 459 60 582
Cuts at: 3015 5364 5620 387
Size: 2349 256 778
Fragments arranged by size:
2349 778 692 582 459 282 256 229
170 154 60
AseI AT'TA_AT
Cuts at: 20 20
Size: 6011
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6011
AvaI C'yCGr_G
Cuts at: 3031 3031
Size: 6011
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2836 2869 2836
Size: 33 5978
BamHI G'GATC_C
Cuts at: 825 3036 825
Size: 2211 3800
BanI G'GyrC_C
Cuts at: 3027 3441 3689 3724 3027
Size: 414 248 35 5314
Fragments arranged by size:
5314 414 248 35
BanII G_rGCy'C
Cuts at: 3025 4055 3025
Size: 1030 4981
BbeI G_GCGC'C
Cuts at: 3445 3693 3445
Size: 248 5763
BbsI GAAGACnn'nnnn_
Cuts at: 1021 1021
Size: 6011
BbvCI CC'TCA_GC
Cuts at: 675 675
Size: 6011
BciVI GTATCCnnnnn_n'
Cuts at: 513 2266 3909 4670 6011 513
Size: 1753 1643 761 1341 513
Fragments arranged by size:
1753 1643 1341 761 513
BclI T'GATC_A
Cuts at: 953 2658 2748 2922 3531 953
Size: 1705 90 174 609 3433
Fragments arranged by size:
3433 1705 609 174 90
BfrBI ATG'CAT
Cuts at: 1458 1946 1458
Size: 488 5523
BglII A'GATC_T
Cuts at: 406 3526 5563 406
Size: 3120 2037 854
Fragments arranged by size:
3120 2037 854
BlpI GC'TnA_GC
Cuts at: 3123 3123
Size: 6011
Bme1580I G_kGCm'C
Cuts at: 300 3636 3729 4785 300
Size: 3336 93 1056 1526
Fragments arranged by size:
3336 1526 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3223 3304 3639 5783 3223
Size: 81 335 2144 3451
Fragments arranged by size:
3451 2144 335 81
BmtI G_CTAG'C
Cuts at: 733 733
Size: 6011
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4378 4378
Size: 6011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 2664 2963 683
Size: 1981 299 3731
Fragments arranged by size:
3731 1981 299
Bpu10I CC'TnA_GC
Cuts at: 675 675
Size: 6011
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 2869 3187 4066 4558 4856 1126
Size: 1743 318 879 492 298 2281
Fragments arranged by size:
2281 1743 879 492 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 5715 5715
Size: 6011
BsaAI yAC'GTr
Cuts at: 1994 3364 1994
Size: 1370 4641
BsaBI GATnn'nnATC
Cuts at: 6 1350 2727 3525 5562 6
Size: 1344 1377 798 2037 455
Fragments arranged by size:
2037 1377 1344 798 455
BsaHI Gr'CG_yC
Cuts at: 3442 3690 4392 5888 3442
Size: 248 702 1496 3565
Fragments arranged by size:
3565 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3200 3721 4399 4673 4820 5231 3200
Size: 521 678 274 147 411 3980
Fragments arranged by size:
3980 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 154
Size: 6011
BseYI C'CCAG_C
Cuts at: 265 646 1127 3436 4194 4771 5267 265
Size: 381 481 2309 758 577 496 1009
Fragments arranged by size:
2309 1009 758 577 496 481 381
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2585
Size: 6011
BsiEI CG_ry'CG
Cuts at: 104 3553 3599 4807 104
Size: 3449 46 1208 1308
Fragments arranged by size:
3449 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 300 2273 3025 3803 3993 4785 300
Size: 1973 752 778 190 792 1526
Fragments arranged by size:
1973 1526 792 778 752 190
BsiWI C'GTAC_G
Cuts at: 1994 1994
Size: 6011
BsmI GAATG_Cn'
Cuts at: 322 1934 2109 322
Size: 1612 175 4224
Fragments arranged by size:
4224 1612 175
Bsp1286I G_dGCh'C
Cuts at: 300 2273 3025 3636 3729 3803 3993 4055 4785
Size: 1973 752 611 93 74 190 62 730
Cuts at: 4785 300
Size: 1526
Fragments arranged by size:
1973 1526 752 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5231 5231
Size: 6011
BspHI T'CATG_A
Cuts at: 5187 5187
Size: 6011
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 1153 3061 3275 3577 3958 556
Size: 597 1908 214 302 381 2609
Fragments arranged by size:
2609 1908 597 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4303 4357 5841 6006 50
Size: 4253 54 1484 165 55
Fragments arranged by size:
4253 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3923 3923
Size: 6011
BsrFI r'CCGG_y
Cuts at: 4009 4190 4399 4009
Size: 181 209 5621
Fragments arranged by size:
5621 209 181
BsrGI T'GTAC_A
Cuts at: 1498 1498
Size: 6011
BssSI C'ACGA_G
Cuts at: 470 4282 4640 470
Size: 3812 358 1841
Fragments arranged by size:
3812 1841 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3388 3388
Size: 6011
BstBI TT'CG_AA
Cuts at: 4372 4372
Size: 6011
BstXI CCAn_nnnn'nTGG
Cuts at: 2524 2524
Size: 6011
BstYI r'GATC_y
Cuts at: 1 406 825 3036 3503 3526 3861 4107 5119
Size: 405 419 2211 467 23 335 246 1012
Cuts at: 5119 5205 5217 5563 1
Size: 86 12 346 449
Fragments arranged by size:
2211 1012 467 449 419 405 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 151 107
Size: 44 5967
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3961 4142 4305 3961
Size: 181 163 5667
Fragments arranged by size:
5667 181 163
BtsI GCAGTG_nn'
Cuts at: 1125 1152 1195 3254 1125
Size: 27 43 2059 3882
Fragments arranged by size:
3882 2059 43 27
DraI TTT'AAA
Cuts at: 1032 3010 1032
Size: 1978 4033
DrdI GACnn_nn'nnGTC
Cuts at: 3717 4575 3717
Size: 858 5153
EaeI y'GGCC_r
Cuts at: 3596 3770 4161 4188 5533 3596
Size: 174 391 27 1345 4074
Fragments arranged by size:
4074 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3596 3596
Size: 6011
EarI CTCTTCn'nnn_
Cuts at: 131 4034 4244 131
Size: 3903 210 1898
Fragments arranged by size:
3903 1898 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4219 4529 4675 5821 4219
Size: 310 146 1146 4409
Fragments arranged by size:
4409 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3023 3023
Size: 6011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 632 674 683 689 1023 1613 1678 2664
Size: 237 42 9 6 334 590 65 986
Cuts at: 2664 2857 2879 2963 3836 4268 5015 5283 395
Size: 193 22 84 873 432 747 268 1123
Fragments arranged by size:
1123 986 873 747 590 432 334 268
237 193 84 65 42 22 9 6
EcoO109I rG'GnC_Cy
Cuts at: 3150 3150
Size: 6011
EcoRI G'AATT_C
Cuts at: 3015 3015
Size: 6011
EcoRV GAT'ATC
Cuts at: 218 218
Size: 6011
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4030 4030
Size: 6011
FspI TGC'GCA
Cuts at: 3792 3792
Size: 6011
HaeII r_GCGC'y
Cuts at: 3445 3693 4715 5694 3445
Size: 248 1022 979 3762
Fragments arranged by size:
3762 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 2655 2662 2687 2694 3853 3883 3885
Size: 32 2232 7 25 7 1159 30 2
Cuts at: 3885 3915 391
Size: 30 2487
Fragments arranged by size:
2487 2232 1159 32 30 30 25 7
7 2
HincII GTy'rAC
Cuts at: 261 340 2206 3050 261
Size: 79 1866 844 3222
Fragments arranged by size:
3222 1866 844 79
HpaI GTT'AAC
Cuts at: 261 261
Size: 6011
KasI G'GCGC_C
Cuts at: 3441 3689 3441
Size: 248 5763
KpnI G_GTAC'C
Cuts at: 3031 3031
Size: 6011
MluI A'CGCG_T
Cuts at: 5444 5444
Size: 6011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 379 1614 1802 4682 4866 379
Size: 1235 188 2880 184 1524
Fragments arranged by size:
2880 1524 1235 188 184
MscI TGG'CCA
Cuts at: 3772 3772
Size: 6011
MslI CAynn'nnrTG
Cuts at: 4000 4127 4000
Size: 127 5884
MspA1I CmG'CkG
Cuts at: 679 3122 3436 3796 4809 5054 679
Size: 2443 314 360 1013 245 1636
Fragments arranged by size:
2443 1636 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4192 4192
Size: 6011
NarI GG'CG_CC
Cuts at: 3442 3690 3442
Size: 248 5763
NcoI C'CATG_G
Cuts at: 107 151 107
Size: 44 5967
NdeI CA'TA_TG
Cuts at: 1286 1320 2054 1286
Size: 34 734 5243
Fragments arranged by size:
5243 734 34
NgoMIV G'CCGG_C
Cuts at: 4190 4190
Size: 6011
NheI G'CTAG_C
Cuts at: 729 729
Size: 6011
NsiI A_TGCA'T
Cuts at: 1460 1948 1460
Size: 488 5523
NspI r_CATG'y
Cuts at: 1273 1529 3064 3564 4471 1273
Size: 256 1535 500 907 2813
Fragments arranged by size:
2813 1535 907 500 256
PciI A'CATG_T
Cuts at: 1269 4467 1269
Size: 3198 2813
PfoI T'CCnGG_A
Cuts at: 1001 1602 2940 1001
Size: 601 1338 4072
Fragments arranged by size:
4072 1338 601
PmeI GTTT'AAAC
Cuts at: 3010 3010
Size: 6011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 564 596 5150 5182 564
Size: 32 4554 32 1393
Fragments arranged by size:
4554 1393 32 32
PsiI TTA'TAA
Cuts at: 1573 1573
Size: 6011
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1599 1631 1599
Size: 32 5979
PstI C_TGCA'G
Cuts at: 1517 3058 3743 1517
Size: 1541 685 3785
Fragments arranged by size:
3785 1541 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6011
PvuII CAG'CTG
Cuts at: 679 3436 3796 679
Size: 2757 360 2894
Fragments arranged by size:
2894 2757 360
RsrII CG'GwC_CG
Cuts at: 4206 4206
Size: 6011
SacI G_AGCT'C
Cuts at: 3025 3025
Size: 6011
SalI G'TCGA_C
Cuts at: 3048 3048
Size: 6011
SapI GCTCTTCn'nnn_
Cuts at: 4034 4244 4034
Size: 210 5801
SbfI CC_TGCA'GG
Cuts at: 3058 3058
Size: 6011
ScaI AGT'ACT
Cuts at: 5528 5528
Size: 6011
SexAI A'CCwGG_T
Cuts at: 447 1305 447
Size: 858 5153
SfcI C'TryA_G
Cuts at: 33 1513 2004 2031 3054 3739 4732 4923 33
Size: 1480 491 27 1023 685 993 191 1121
Fragments arranged by size:
1480 1121 1023 993 685 491 191 27
SfoI GGC'GCC
Cuts at: 3443 3691 3443
Size: 248 5763
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6011
SmaI CCC'GGG
Cuts at: 3033 3033
Size: 6011
SmlI C'TyrA_G
Cuts at: 1105 2199 2848 3166 4081 4573 4835 1105
Size: 1094 649 318 915 492 262 2281
Fragments arranged by size:
2281 1094 915 649 492 318 262
SnaBI TAC'GTA
Cuts at: 1994 1994
Size: 6011
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6011
SphI G_CATG'C
Cuts at: 3064 3564 3064
Size: 500 5511
SspI AAT'ATT
Cuts at: 246 2559 2709 246
Size: 2313 150 3548
Fragments arranged by size:
3548 2313 150
StyI C'CwwG_G
Cuts at: 107 151 540 1708 3145 3499 107
Size: 44 389 1168 1437 354 2619
Fragments arranged by size:
2619 1437 1168 389 354 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3594 3727 4186 4395 3594
Size: 133 459 209 5210
Fragments arranged by size:
5210 459 209 133
TatI w'GTAC_w
Cuts at: 907 1204 1498 5526 907
Size: 297 294 4028 1392
Fragments arranged by size:
4028 1392 297 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5528 5560 5528
Size: 32 5979
Tth111I GACn'n_nGTC
Cuts at: 3808 3808
Size: 6011
XbaI T'CTAG_A
Cuts at: 403 3042 403
Size: 2639 3372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3506 3506
Size: 6011
XmaI C'CCGG_G
Cuts at: 3031 3031
Size: 6011
XmnI GAAnn'nnTTC
Cuts at: 1271 1271
Size: 6011
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PciI PfoI
PmeI PpiI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI ApaI AscI AvrII
BcgI BglI BmgBI BsaXI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
MfeI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PspOMI PspXI SacII SanDI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI