Restriction map for
pF1KA1579
(Circular) (Six-base) MAPSORT of: pF1KA1579.seq Check: 6569 from: 1 to: 5147
pF1KA1579 5147 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 723 2138 2185 723
Size: 1415 47 3685
Fragments arranged by size:
3685 1415 47
Acc65I G'GTAC_C
Cuts at: 2163 2163
Size: 5147
AclI AA'CG_TT
Cuts at: 4987 4987
Size: 5147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 764 983 1387 1639 2972 3404 3757 4419
Size: 262 219 404 252 1333 432 353 662
Cuts at: 4419 502
Size: 1230
Fragments arranged by size:
1333 1230 662 432 404 353 262 252
219
AflIII A'CryG_T
Cuts at: 1080 2499 4299 4580 1080
Size: 1419 1800 281 1647
Fragments arranged by size:
1800 1647 1419 281
AlwNI CAG_nnn'CTG
Cuts at: 350 1750 2452 3890 350
Size: 1400 702 1438 1607
Fragments arranged by size:
1607 1438 1400 702
ApaI G_GGCC'C
Cuts at: 205 205
Size: 5147
ApaLI G'TGCA_C
Cuts at: 3985 3985
Size: 5147
ApoI r'AATT_y
Cuts at: 857 1629 1796 1824 2151 4500 4756 857
Size: 772 167 28 327 2349 256 1248
Fragments arranged by size:
2349 1248 772 327 256 167 28
AseI AT'TA_AT
Cuts at: 20 1333 20
Size: 1313 3834
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
AvaI C'yCGr_G
Cuts at: 2167 2167
Size: 5147
BamHI G'GATC_C
Cuts at: 2172 2172
Size: 5147
BanI G'GyrC_C
Cuts at: 226 2163 2577 2825 2860 226
Size: 1937 414 248 35 2513
Fragments arranged by size:
2513 1937 414 248 35
BanII G_rGCy'C
Cuts at: 205 788 1388 2161 3191 205
Size: 583 600 773 1030 2161
Fragments arranged by size:
2161 1030 773 600 583
BbeI G_GCGC'C
Cuts at: 230 2581 2829 230
Size: 2351 248 2548
Fragments arranged by size:
2548 2351 248
BbvCI CC'TCA_GC
Cuts at: 207 207
Size: 5147
BciVI GTATCCnnnnn_n'
Cuts at: 3045 4101 5147 3045
Size: 1056 1046 3045
Fragments arranged by size:
3045 1056 1046
BclI T'GATC_A
Cuts at: 2667 2667
Size: 5147
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5147
BglII A'GATC_T
Cuts at: 2662 4699 2662
Size: 2037 3110
BlpI GC'TnA_GC
Cuts at: 301 2259 301
Size: 1958 3189
Bme1580I G_kGCm'C
Cuts at: 205 557 1580 2095 2772 2865 3989 205
Size: 352 1023 515 677 93 1124 1363
Fragments arranged by size:
1363 1124 1023 677 515 352 93
BmrI ACTGGGnnnn_n'
Cuts at: 818 1179 1838 2359 2440 2775 4919 818
Size: 361 659 521 81 335 2144 1046
Fragments arranged by size:
2144 1046 659 521 361 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3514 3514
Size: 5147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1055 730
Size: 325 4822
Bpu10I CC'TnA_GC
Cuts at: 207 207
Size: 5147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 3202 3916 4214 2323
Size: 879 714 298 3256
Fragments arranged by size:
3256 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4851 4851
Size: 5147
BsaAI yAC'GTr
Cuts at: 2500 2500
Size: 5147
BsaBI GATnn'nnATC
Cuts at: 6 767 2661 4698 6
Size: 761 1894 2037 455
Fragments arranged by size:
2037 1894 761 455
BsaHI Gr'CG_yC
Cuts at: 227 2578 2826 3528 5024 227
Size: 2351 248 702 1496 350
Fragments arranged by size:
2351 1496 702 350 248
BsaWI w'CCGG_w
Cuts at: 2336 2857 3535 3946 4093 4367 2336
Size: 521 678 411 147 274 3116
Fragments arranged by size:
3116 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 277
Size: 5147
BseYI C'CCAG_C
Cuts at: 171 1389 1567 2572 3330 3995 4403 171
Size: 1218 178 1005 758 665 408 915
Fragments arranged by size:
1218 1005 915 758 665 408 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 1394 238
Size: 1156 3991
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3965 104
Size: 2585 46 1230 1286
Fragments arranged by size:
2585 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 788 1032 2161 2939 3129 3989 788
Size: 244 1129 778 190 860 1946
Fragments arranged by size:
1946 1129 860 778 244 190
BsmI GAATG_Cn'
Cuts at: 1907 2082 1907
Size: 175 4972
BsmBI CGTCTCn'nnnn_
Cuts at: 119 119
Size: 5147
Bsp1286I G_dGCh'C
Cuts at: 205 557 788 1032 1388 1580 2095 2161 2772
Size: 352 231 244 356 192 515 66 611
Cuts at: 2772 2865 2939 3129 3191 3989 205
Size: 93 74 190 62 798 1363
Fragments arranged by size:
1363 798 611 515 356 352 244 231
192 190 93 74 66 62
BspHI T'CATG_A
Cuts at: 363 3579 363
Size: 3216 1931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 341 2197 2411 2713 3094 341
Size: 1856 214 302 381 2394
Fragments arranged by size:
2394 1856 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3439 3493 4977 5142 50
Size: 3389 54 1484 165 55
Fragments arranged by size:
3389 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1553 1961 3059 1553
Size: 408 1098 3641
Fragments arranged by size:
3641 1098 408
BsrFI r'CCGG_y
Cuts at: 3145 3326 3145
Size: 181 4966
BssSI C'ACGA_G
Cuts at: 220 3418 4126 220
Size: 3198 708 1241
Fragments arranged by size:
3198 1241 708
BstAPI GCAn_nnn'nTGC
Cuts at: 288 748 2524 288
Size: 460 1776 2911
Fragments arranged by size:
2911 1776 460
BstBI TT'CG_AA
Cuts at: 3508 3508
Size: 5147
BstXI CCAn_nnnn'nTGG
Cuts at: 1192 1192
Size: 5147
BstYI r'GATC_y
Cuts at: 1 2172 2639 2662 2997 3243 3549 3561 3647
Size: 2171 467 23 335 246 306 12 86
Cuts at: 3647 4699 1
Size: 1052 449
Fragments arranged by size:
2171 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 2139 2139
Size: 5147
Bsu36I CC'TnA_GG
Cuts at: 260 260
Size: 5147
BtgI C'CryG_G
Cuts at: 107 1607 107
Size: 1500 3647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3097 3278 3441 3097
Size: 181 163 4803
Fragments arranged by size:
4803 181 163
BtsI GCAGTG_nn'
Cuts at: 938 947 2390 938
Size: 9 1443 3695
Fragments arranged by size:
3695 1443 9
DraI TTT'AAA
Cuts at: 2146 2146
Size: 5147
DrdI GACnn_nn'nnGTC
Cuts at: 508 2853 4197 508
Size: 2345 1344 1458
Fragments arranged by size:
2345 1458 1344
EaeI y'GGCC_r
Cuts at: 645 2732 2906 3297 3324 4669 645
Size: 2087 174 391 27 1345 1123
Fragments arranged by size:
2087 1345 1123 391 174 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5147
EarI CTCTTCn'nnn_
Cuts at: 569 839 959 1886 3170 3380 569
Size: 270 120 927 1284 210 2336
Fragments arranged by size:
2336 1284 927 270 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3355 4097 4243 4957 3355
Size: 742 146 714 3545
Fragments arranged by size:
3545 742 714 146
EcoICRI GAG'CTC
Cuts at: 786 2159 786
Size: 1373 3774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 730 764 983 1055 1387 1639 2972 3404
Size: 228 34 219 72 332 252 1333 432
Cuts at: 3404 3757 4419 502
Size: 353 662 1230
Fragments arranged by size:
1333 1230 662 432 353 332 252 228
219 72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 337 337
Size: 5147
EcoO109I rG'GnC_Cy
Cuts at: 201 202 2286 201
Size: 1 2084 3062
Fragments arranged by size:
3062 2084 1
EcoRI G'AATT_C
Cuts at: 2151 2151
Size: 5147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 5147
FspI TGC'GCA
Cuts at: 2928 2928
Size: 5147
HaeII r_GCGC'y
Cuts at: 230 1042 2581 2829 4059 4830 230
Size: 812 1539 248 1230 771 547
Fragments arranged by size:
1539 1230 812 771 547 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 462 494 1059 1091 1482 1514 1906 1938 2989
Size: 32 565 32 391 32 392 32 1051
Cuts at: 2989 3019 3021 3051 462
Size: 30 2 30 2558
Fragments arranged by size:
2558 1051 565 392 391 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2076 2186 2076
Size: 110 5037
HindIII A'AGCT_T
Cuts at: 1683 1683
Size: 5147
KasI G'GCGC_C
Cuts at: 226 2577 2825 226
Size: 2351 248 2548
Fragments arranged by size:
2548 2351 248
KpnI G_GTAC'C
Cuts at: 2167 2167
Size: 5147
MluI A'CGCG_T
Cuts at: 4580 4580
Size: 5147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1519 3906 4090 1519
Size: 2387 184 2576
Fragments arranged by size:
2576 2387 184
MscI TGG'CCA
Cuts at: 647 2908 647
Size: 2261 2886
MslI CAynn'nnrTG
Cuts at: 1460 3136 3263 1460
Size: 1676 127 3344
Fragments arranged by size:
3344 1676 127
MspA1I CmG'CkG
Cuts at: 285 294 1393 1516 2258 2572 2932 3716 3961
Size: 9 1099 123 742 314 360 784 245
Cuts at: 3961 285
Size: 1471
Fragments arranged by size:
1471 1099 784 742 360 314 245 123
9
NaeI GCC'GGC
Cuts at: 3328 3328
Size: 5147
NarI GG'CG_CC
Cuts at: 227 2578 2826 227
Size: 2351 248 2548
Fragments arranged by size:
2548 2351 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5147
NgoMIV G'CCGG_C
Cuts at: 3326 3326
Size: 5147
NspI r_CATG'y
Cuts at: 1530 2200 2700 4303 1530
Size: 670 500 1603 2374
Fragments arranged by size:
2374 1603 670 500
PciI A'CATG_T
Cuts at: 4299 4299
Size: 5147
PflMI CCAn_nnn'nTGG
Cuts at: 1981 2056 1981
Size: 75 5072
PfoI T'CCnGG_A
Cuts at: 1032 1032
Size: 5147
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 5115
PspOMI G'GGCC_C
Cuts at: 201 201
Size: 5147
PstI C_TGCA'G
Cuts at: 284 2194 2879 284
Size: 1910 685 2552
Fragments arranged by size:
2552 1910 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5147
PvuII CAG'CTG
Cuts at: 1393 1516 2572 2932 1393
Size: 123 1056 360 3608
Fragments arranged by size:
3608 1056 360 123
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5147
SacI G_AGCT'C
Cuts at: 788 2161 788
Size: 1373 3774
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5147
SapI GCTCTTCn'nnn_
Cuts at: 839 3170 3380 839
Size: 2331 210 2606
Fragments arranged by size:
2606 2331 210
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5147
ScaI AGT'ACT
Cuts at: 4664 4664
Size: 5147
SfcI C'TryA_G
Cuts at: 33 280 630 831 1177 2190 2875 3843 4034
Size: 247 350 201 346 1013 685 968 191
Cuts at: 4034 33
Size: 1146
Fragments arranged by size:
1146 1013 968 685 350 346 247 201
191
SfoI GGC'GCC
Cuts at: 228 2579 2827 228
Size: 2351 248 2548
Fragments arranged by size:
2548 2351 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5147
SmlI C'TyrA_G
Cuts at: 2302 3217 3931 4193 2302
Size: 915 714 262 3256
Fragments arranged by size:
3256 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5147
SphI G_CATG'C
Cuts at: 2200 2700 2200
Size: 500 4647
SspI AAT'ATT
Cuts at: 394 484 609 1350 394
Size: 90 125 741 4191
Fragments arranged by size:
4191 741 125 90
StyI C'CwwG_G
Cuts at: 107 2281 2635 107
Size: 2174 354 2619
Fragments arranged by size:
2619 2174 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2730 2863 3322 3531 2730
Size: 133 459 209 4346
Fragments arranged by size:
4346 459 209 133
TatI w'GTAC_w
Cuts at: 1051 1128 4662 1051
Size: 77 3534 1536
Fragments arranged by size:
3534 1536 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4664 4696 4664
Size: 32 5115
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5147
XbaI T'CTAG_A
Cuts at: 2178 2178
Size: 5147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1614 2642 1614
Size: 1028 4119
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5147
XmnI GAAnn'nnTTC
Cuts at: 590 590
Size: 5147
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BsaXI BsiWI BspEI BsrGI BssHII BstEII ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI