Restriction map for
pF1KA1575
(Circular) (Six-base) MAPSORT of: pF1KA1575.seq Check: 9839 from: 1 to: 6125
pF1KA1575 6125 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3093 3093
Size: 6125
AatII G_ACGT'C
Cuts at: 1415 1415
Size: 6125
AccI GT'mk_AC
Cuts at: 2144 2519 2525 3163 2144
Size: 375 6 638 5106
Fragments arranged by size:
5106 638 375 6
Acc65I G'GTAC_C
Cuts at: 2764 3141 2764
Size: 377 5748
AclI AA'CG_TT
Cuts at: 137 1775 5965 137
Size: 1638 4190 297
Fragments arranged by size:
4190 1638 297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 1196 3950 4382 5129 5397 525
Size: 671 2754 432 747 268 1253
Fragments arranged by size:
2754 1253 747 671 432 268
AfeI AGC'GCT
Cuts at: 602 835 2610 602
Size: 233 1775 4117
Fragments arranged by size:
4117 1775 233
AflIII A'CryG_T
Cuts at: 755 2567 3477 4581 5558 755
Size: 1812 910 1104 977 1322
Fragments arranged by size:
1812 1322 1104 977 910
AgeI A'CCGG_T
Cuts at: 4513 4513
Size: 6125
AleI CACnn'nnGTG
Cuts at: 2566 2566
Size: 6125
AlwNI CAG_nnn'CTG
Cuts at: 804 2095 3430 4997 804
Size: 1291 1335 1567 1932
Fragments arranged by size:
1932 1567 1335 1291
ApaLI G'TGCA_C
Cuts at: 4895 4895
Size: 6125
ApoI r'AATT_y
Cuts at: 3129 5478 5734 3129
Size: 2349 256 3520
Fragments arranged by size:
3520 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6125
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
AvaI C'yCGr_G
Cuts at: 360 493 1130 1327 1642 3145 360
Size: 133 637 197 315 1503 3340
Fragments arranged by size:
3340 1503 637 315 197 133
AvrII C'CTAG_G
Cuts at: 2781 2781
Size: 6125
BamHI G'GATC_C
Cuts at: 2865 3150 2865
Size: 285 5840
BanI G'GyrC_C
Cuts at: 700 1312 1638 1753 1846 1954 2189 2764 3141
Size: 612 326 115 93 108 235 575 377
Cuts at: 3141 3555 3803 3838 700
Size: 414 248 35 2987
Fragments arranged by size:
2987 612 575 414 377 326 248 235
115 108 93 35
BanII G_rGCy'C
Cuts at: 400 640 1867 2367 2618 3112 3139 4169 400
Size: 240 1227 500 251 494 27 1030 2356
Fragments arranged by size:
2356 1227 1030 500 494 251 240 27
BbeI G_GCGC'C
Cuts at: 1958 3559 3807 1958
Size: 1601 248 4276
Fragments arranged by size:
4276 1601 248
BbsI GAAGACnn'nnnn_
Cuts at: 194 1348 1430 2497 2724 194
Size: 1154 82 1067 227 3595
Fragments arranged by size:
3595 1154 1067 227 82
BbvCI CC'TCA_GC
Cuts at: 776 776
Size: 6125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2818 2852 3043 3077 90
Size: 34 2694 34 191 34 3138
Fragments arranged by size:
3138 2694 191 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1295 4023 4784 6125 1295
Size: 2728 761 1341 1295
Fragments arranged by size:
2728 1341 1295 761
BclI T'GATC_A
Cuts at: 3645 3645
Size: 6125
BglI GCCn_nnn'nGGC
Cuts at: 2220 2220
Size: 6125
BglII A'GATC_T
Cuts at: 1600 3640 5677 1600
Size: 2040 2037 2048
Fragments arranged by size:
2048 2040 2037
BlpI GC'TnA_GC
Cuts at: 2612 3079 3237 2612
Size: 467 158 5500
Fragments arranged by size:
5500 467 158
Bme1580I G_kGCm'C
Cuts at: 1317 1328 1643 2192 2895 3750 3843 4899 1317
Size: 11 315 549 703 855 93 1056 2543
Fragments arranged by size:
2543 1056 855 703 549 315 93 11
BmrI ACTGGGnnnn_n'
Cuts at: 403 1716 2149 3337 3418 3753 5897 403
Size: 1313 433 1188 81 335 2144 631
Fragments arranged by size:
2144 1313 1188 631 433 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4492 4492
Size: 6125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 1559 2830 797
Size: 762 1271 4092
Fragments arranged by size:
4092 1271 762
Bpu10I CC'TnA_GC
Cuts at: 776 776
Size: 6125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3301 4180 4672 4970 3301
Size: 879 492 298 4456
Fragments arranged by size:
4456 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5829 5829
Size: 6125
BsaAI yAC'GTr
Cuts at: 2523 3024 3478 2523
Size: 501 454 5170
Fragments arranged by size:
5170 501 454
BsaBI GATnn'nnATC
Cuts at: 6 3639 5676 6
Size: 3633 2037 455
Fragments arranged by size:
3633 2037 455
BsaHI Gr'CG_yC
Cuts at: 983 1412 1955 3556 3804 4506 6002 983
Size: 429 543 1601 248 702 1496 1106
Fragments arranged by size:
1601 1496 1106 702 543 429 248
BsaWI w'CCGG_w
Cuts at: 1296 1540 2387 3314 3835 4513 4787 4934 5345
Size: 244 847 927 521 678 274 147 411
Cuts at: 5345 1296
Size: 2076
Fragments arranged by size:
2076 927 847 678 521 411 274 244
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1554 1584 1554
Size: 30 6095
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1370 1562 2341 2355 2692 1370
Size: 192 779 14 337 4803
Fragments arranged by size:
4803 779 337 192 14
BseYI C'CCAG_C
Cuts at: 603 797 927 1148 1316 1806 2088 2578 3550
Size: 194 130 221 168 490 282 490 972
Cuts at: 3550 4308 4885 5381 603
Size: 758 577 496 1347
Fragments arranged by size:
1347 972 758 577 496 490 490 282
221 194 168 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 1817 804
Size: 1013 5112
BsiEI CG_ry'CG
Cuts at: 104 1123 2235 3667 3713 4921 104
Size: 1019 1112 1432 46 1208 1308
Fragments arranged by size:
1432 1308 1208 1112 1019 46
BsiHKAI G_wGCw'C
Cuts at: 286 1538 1867 3139 3917 4107 4899 286
Size: 1252 329 1272 778 190 792 1512
Fragments arranged by size:
1512 1272 1252 792 778 329 190
BsmI GAATG_Cn'
Cuts at: 2041 2041
Size: 6125
BsmBI CGTCTCn'nnnn_
Cuts at: 522 522
Size: 6125
Bsp1286I G_dGCh'C
Cuts at: 286 400 640 1317 1328 1538 1643 1867 2192
Size: 114 240 677 11 210 105 224 325
Cuts at: 2192 2367 2618 2895 3112 3139 3750 3843 3917
Size: 175 251 277 217 27 611 93 74
Cuts at: 3917 4107 4169 4899 286
Size: 190 62 730 1512
Fragments arranged by size:
1512 730 677 611 325 277 251 240
224 217 210 190 175 114 105 93
74 62 27 11
BspEI T'CCGG_A
Cuts at: 1296 1540 2387 5345 1296
Size: 244 847 2958 2076
Fragments arranged by size:
2958 2076 847 244
BspHI T'CATG_A
Cuts at: 5301 5301
Size: 6125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 854 2752 3093 3175 3389 3691 4072 854
Size: 1898 341 82 214 302 381 2907
Fragments arranged by size:
2907 1898 381 341 302 214 82
BsrBI CCG'CTC
Cuts at: 50 4417 4471 5955 6120 50
Size: 4367 54 1484 165 55
Fragments arranged by size:
4367 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 948 2102 3097 4037 948
Size: 1154 995 940 3036
Fragments arranged by size:
3036 1154 995 940
BsrFI r'CCGG_y
Cuts at: 2230 2637 4123 4304 4513 2230
Size: 407 1486 181 209 3842
Fragments arranged by size:
3842 1486 407 209 181
BsrGI T'GTAC_A
Cuts at: 758 758
Size: 6125
BssSI C'ACGA_G
Cuts at: 4396 4754 4396
Size: 358 5767
BstAPI GCAn_nnn'nTGC
Cuts at: 3502 3502
Size: 6125
BstBI TT'CG_AA
Cuts at: 4486 4486
Size: 6125
BstEII G'GTnAC_C
Cuts at: 1068 3028 1068
Size: 1960 4165
BstXI CCAn_nnnn'nTGG
Cuts at: 1503 1503
Size: 6125
BstYI r'GATC_y
Cuts at: 1 1196 1600 2344 2865 3150 3617 3640 3975
Size: 1195 404 744 521 285 467 23 335
Cuts at: 3975 4221 5233 5319 5331 5677 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1195 1012 744 521 467 449 404 346
335 285 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2096 2096
Size: 6125
BtgI C'CryG_G
Cuts at: 970 1259 1496 970
Size: 289 237 5599
Fragments arranged by size:
5599 289 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2649 2843 4075 4256 4419 2649
Size: 194 1232 181 163 4355
Fragments arranged by size:
4355 1232 194 181 163
BtsI GCAGTG_nn'
Cuts at: 1616 2895 3368 1616
Size: 1279 473 4373
Fragments arranged by size:
4373 1279 473
ClaI AT'CG_AT
Cuts at: 299 299
Size: 6125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1415 1450 1415
Size: 35 6090
DraI TTT'AAA
Cuts at: 3124 3124
Size: 6125
DraIII CAC_nnn'GTG
Cuts at: 270 2902 270
Size: 2632 3493
DrdI GACnn_nn'nnGTC
Cuts at: 2515 3831 4689 2515
Size: 1316 858 3951
Fragments arranged by size:
3951 1316 858
EaeI y'GGCC_r
Cuts at: 1120 1262 1913 2232 2601 3710 3884 4275 4302
Size: 142 651 319 369 1109 174 391 27
Cuts at: 4302 5647 1120
Size: 1345 1598
Fragments arranged by size:
1598 1345 1109 651 391 369 319 174
142 27
EagI C'GGCC_G
Cuts at: 1120 2232 3710 1120
Size: 1112 1478 3535
Fragments arranged by size:
3535 1478 1112
EarI CTCTTCn'nnn_
Cuts at: 242 1655 4148 4358 242
Size: 1413 2493 210 2009
Fragments arranged by size:
2493 2009 1413 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4333 4643 4789 5935 4333
Size: 310 146 1146 4523
Fragments arranged by size:
4523 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1865 3137 1865
Size: 1272 4853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 797 1196 1559 2830 3950 4382 5129 5397
Size: 272 399 363 1271 1120 432 747 268
Cuts at: 5397 525
Size: 1253
Fragments arranged by size:
1271 1253 1120 747 432 399 363 272
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2067 2674 2067
Size: 607 5518
EcoO109I rG'GnC_Cy
Cuts at: 357 2048 2137 2376 3264 357
Size: 1691 89 239 888 3218
Fragments arranged by size:
3218 1691 888 239 89
EcoRI G'AATT_C
Cuts at: 3129 3129
Size: 6125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 6125
FspI TGC'GCA
Cuts at: 954 3906 954
Size: 2952 3173
HaeII r_GCGC'y
Cuts at: 604 837 1958 2612 3559 3807 4829 5808 604
Size: 233 1121 654 947 248 1022 979 921
Fragments arranged by size:
1121 1022 979 947 921 654 248 233
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1153 1185 1357 1389 1592 1624 2329 2361 2935
Size: 32 172 32 203 32 705 32 574
Cuts at: 2935 2967 3967 3997 3999 4029 1153
Size: 32 1000 30 2 30 3249
Fragments arranged by size:
3249 1000 705 574 203 172 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3164 3164
Size: 6125
HindIII A'AGCT_T
Cuts at: 2512 2512
Size: 6125
KasI G'GCGC_C
Cuts at: 1954 3555 3803 1954
Size: 1601 248 4276
Fragments arranged by size:
4276 1601 248
KpnI G_GTAC'C
Cuts at: 2768 3145 2768
Size: 377 5748
MluI A'CGCG_T
Cuts at: 5558 5558
Size: 6125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1367 4796 4980 1367
Size: 3429 184 2512
Fragments arranged by size:
3429 2512 184
MscI TGG'CCA
Cuts at: 2603 3886 2603
Size: 1283 4842
MslI CAynn'nnrTG
Cuts at: 2438 2566 4114 4241 2438
Size: 128 1548 127 4322
Fragments arranged by size:
4322 1548 128 127
MspA1I CmG'CkG
Cuts at: 146 804 1261 1348 1725 3016 3236 3550 3910
Size: 658 457 87 377 1291 220 314 360
Cuts at: 3910 4923 5168 146
Size: 1013 245 1103
Fragments arranged by size:
1291 1103 1013 658 457 377 360 314
245 220 87
NaeI GCC'GGC
Cuts at: 2639 4306 2639
Size: 1667 4458
NarI GG'CG_CC
Cuts at: 1955 3556 3804 1955
Size: 1601 248 4276
Fragments arranged by size:
4276 1601 248
NgoMIV G'CCGG_C
Cuts at: 2637 4304 2637
Size: 1667 4458
NspI r_CATG'y
Cuts at: 174 759 1336 1683 1900 3178 3678 4585 174
Size: 585 577 347 217 1278 500 907 1714
Fragments arranged by size:
1714 1278 907 585 577 500 347 217
PasI CC'CwG_GG
Cuts at: 677 991 1046 2265 677
Size: 314 55 1219 4537
Fragments arranged by size:
4537 1219 314 55
PciI A'CATG_T
Cuts at: 755 4581 755
Size: 3826 2299
PflMI CCAn_nnn'nTGG
Cuts at: 270 1582 2206 270
Size: 1312 624 4189
Fragments arranged by size:
4189 1312 624
PfoI T'CCnGG_A
Cuts at: 341 1575 2807 341
Size: 1234 1232 3659
Fragments arranged by size:
3659 1234 1232
PmeI GTTT'AAAC
Cuts at: 3124 3124
Size: 6125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 460 492 5264 5296 460
Size: 32 4772 32 1289
Fragments arranged by size:
4772 1289 32 32
PpuMI rG'GwC_Cy
Cuts at: 357 2048 2137 2376 357
Size: 1691 89 239 4106
Fragments arranged by size:
4106 1691 239 89
PshAI GACnn'nnGTC
Cuts at: 2497 2497
Size: 6125
PstI C_TGCA'G
Cuts at: 1161 2648 3172 3857 1161
Size: 1487 524 685 3429
Fragments arranged by size:
3429 1487 685 524
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6125
PvuII CAG'CTG
Cuts at: 146 3550 3910 146
Size: 3404 360 2361
Fragments arranged by size:
3404 2361 360
RsrII CG'GwC_CG
Cuts at: 4320 4320
Size: 6125
SacI G_AGCT'C
Cuts at: 1867 3139 1867
Size: 1272 4853
SacII CC_GC'GG
Cuts at: 1262 1262
Size: 6125
SalI G'TCGA_C
Cuts at: 3162 3162
Size: 6125
SanDI GG'GwC_CC
Cuts at: 357 357
Size: 6125
SapI GCTCTTCn'nnn_
Cuts at: 4148 4358 4148
Size: 210 5915
SbfI CC_TGCA'GG
Cuts at: 3172 3172
Size: 6125
ScaI AGT'ACT
Cuts at: 5642 5642
Size: 6125
SfcI C'TryA_G
Cuts at: 33 584 786 1157 1596 1868 2644 3168 3853
Size: 551 202 371 439 272 776 524 685
Cuts at: 3853 4846 5037 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 776 685 551 524 439 371
272 202 191
SfoI GGC'GCC
Cuts at: 1956 3557 3805 1956
Size: 1601 248 4276
Fragments arranged by size:
4276 1601 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
SmaI CCC'GGG
Cuts at: 362 1329 1644 3147 362
Size: 967 315 1503 3340
Fragments arranged by size:
3340 1503 967 315
SmlI C'TyrA_G
Cuts at: 3280 4195 4687 4949 3280
Size: 915 492 262 4456
Fragments arranged by size:
4456 915 492 262
SnaBI TAC'GTA
Cuts at: 2523 2523
Size: 6125
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6125
SphI G_CATG'C
Cuts at: 1336 3178 3678 1336
Size: 1842 500 3783
Fragments arranged by size:
3783 1842 500
SrfI GCCC'GGGC
Cuts at: 1329 1644 1329
Size: 315 5810
StuI AGG'CCT
Cuts at: 618 766 618
Size: 148 5977
StyI C'CwwG_G
Cuts at: 441 887 1061 1289 2781 3259 3613 441
Size: 446 174 228 1492 478 354 2953
Fragments arranged by size:
2953 1492 478 446 354 228 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 856 912 1162 1623 2168 3016 3047 3708 3841
Size: 56 250 461 545 848 31 661 133
Cuts at: 3841 4300 4509 856
Size: 459 209 2472
Fragments arranged by size:
2472 848 661 545 461 459 250 209
133 56 31
TatI w'GTAC_w
Cuts at: 758 5640 758
Size: 4882 1243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1118 1150 2220 2252 5642 5674 1118
Size: 32 1070 32 3390 32 1569
Fragments arranged by size:
3390 1569 1070 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3922 3922
Size: 6125
XbaI T'CTAG_A
Cuts at: 3156 3156
Size: 6125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 298 3620 298
Size: 3322 2803
XmaI C'CCGG_G
Cuts at: 360 1327 1642 3145 360
Size: 967 315 1503 3340
Fragments arranged by size:
3340 1503 967 315
ZraI GAC'GTC
Cuts at: 1413 1413
Size: 6125
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI ApaI AscI BaeI BfrBI
BmgBI BmtI BsiWI BssHII BstZ17I EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspOMI PspXI PsrI SexAI SfiI
SgrAI SspI SwaI XhoI XmnI