Restriction map for
pF1KA1568
(Circular) (Six-base) MAPSORT of: pF1KA1568.seq Check: 9302 from: 1 to: 7247
pF1KA1568 7247 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 709 4285 709
Size: 3576 3671
Acc65I G'GTAC_C
Cuts at: 721 1741 4263 721
Size: 1020 2522 3705
Fragments arranged by size:
3705 2522 1020
AclI AA'CG_TT
Cuts at: 1254 7087 1254
Size: 5833 1414
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2324 5072 5504 6251 6519 2117
Size: 207 2748 432 747 268 2845
Fragments arranged by size:
2845 2748 747 432 268 207
AflIII A'CryG_T
Cuts at: 4599 5703 6680 4599
Size: 1104 977 5166
Fragments arranged by size:
5166 1104 977
AgeI A'CCGG_T
Cuts at: 5635 5635
Size: 7247
AhdI GACnn_n'nnGTC
Cuts at: 2135 2135
Size: 7247
AleI CACnn'nnGTG
Cuts at: 2668 2668
Size: 7247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3572 3572
Size: 7247
AlwNI CAG_nnn'CTG
Cuts at: 1630 1942 4552 6119 1630
Size: 312 2610 1567 2758
Fragments arranged by size:
2758 2610 1567 312
ApaLI G'TGCA_C
Cuts at: 394 6017 394
Size: 5623 1624
ApoI r'AATT_y
Cuts at: 835 1536 2163 2946 4251 6600 6856 835
Size: 701 627 783 1305 2349 256 1226
Fragments arranged by size:
2349 1305 1226 783 701 627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7247
AvaI C'yCGr_G
Cuts at: 578 4267 578
Size: 3689 3558
AvrII C'CTAG_G
Cuts at: 4135 4135
Size: 7247
BamHI G'GATC_C
Cuts at: 370 2388 3304 3832 4123 4272 370
Size: 2018 916 528 291 149 3345
Fragments arranged by size:
3345 2018 916 528 291 149
BanI G'GyrC_C
Cuts at: 721 973 1741 2707 3419 4053 4263 4677 4925
Size: 252 768 966 712 634 210 414 248
Cuts at: 4925 4960 721
Size: 35 3008
Fragments arranged by size:
3008 966 768 712 634 414 252 248
210 35
BanII G_rGCy'C
Cuts at: 248 1044 4210 4261 5291 248
Size: 796 3166 51 1030 2204
Fragments arranged by size:
3166 2204 1030 796 51
BbeI G_GCGC'C
Cuts at: 4681 4929 4681
Size: 248 6999
BbsI GAAGACnn'nnnn_
Cuts at: 169 2020 2836 169
Size: 1851 816 4580
Fragments arranged by size:
4580 1851 816
BciVI GTATCCnnnnn_n'
Cuts at: 664 5145 5906 7247 664
Size: 4481 761 1341 664
Fragments arranged by size:
4481 1341 761 664
BclI T'GATC_A
Cuts at: 3222 4767 3222
Size: 1545 5702
BglII A'GATC_T
Cuts at: 2435 3110 3192 4762 6799 2435
Size: 675 82 1570 2037 2883
Fragments arranged by size:
2883 2037 1570 675 82
BlpI GC'TnA_GC
Cuts at: 4359 4359
Size: 7247
Bme1580I G_kGCm'C
Cuts at: 398 2322 3424 4058 4872 4965 6021 398
Size: 1924 1102 634 814 93 1056 1624
Fragments arranged by size:
1924 1624 1102 1056 814 634 93
BmrI ACTGGGnnnn_n'
Cuts at: 2088 3334 4064 4459 4540 4875 7019 2088
Size: 1246 730 395 81 335 2144 2316
Fragments arranged by size:
2316 2144 1246 730 395 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5614 5614
Size: 7247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 583 838 1247 1345 1623 1652 2384 3680
Size: 17 255 409 98 278 29 732 1296
Cuts at: 3680 3743 4067 566
Size: 63 324 3746
Fragments arranged by size:
3746 1296 732 409 324 278 255 98
63 29 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1070 4423 5302 5794 6092 1070
Size: 3353 879 492 298 2225
Fragments arranged by size:
3353 2225 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1223 1918 6951 1223
Size: 695 5033 1519
Fragments arranged by size:
5033 1519 695
BsaAI yAC'GTr
Cuts at: 4600 4600
Size: 7247
BsaBI GATnn'nnATC
Cuts at: 6 4761 6798 6
Size: 4755 2037 455
Fragments arranged by size:
4755 2037 455
BsaHI Gr'CG_yC
Cuts at: 4678 4926 5628 7124 4678
Size: 248 702 1496 4801
Fragments arranged by size:
4801 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4436 4957 5635 5909 6056 6467 4436
Size: 521 678 274 147 411 5216
Fragments arranged by size:
5216 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3552 3582 3552
Size: 30 7217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2384 2387 2819 3873 4057 2384
Size: 3 432 1054 184 5574
Fragments arranged by size:
5574 1054 432 184 3
BseYI C'CCAG_C
Cuts at: 364 753 1190 1377 2714 2863 4672 5430 6007
Size: 389 437 187 1337 149 1809 758 577
Cuts at: 6007 6503 364
Size: 496 1108
Fragments arranged by size:
1809 1337 1108 758 577 496 437 389
187 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 2017 2585 3956 1628
Size: 389 568 1371 4919
Fragments arranged by size:
4919 1371 568 389
BsiEI CG_ry'CG
Cuts at: 104 4789 4835 6043 104
Size: 4685 46 1208 1308
Fragments arranged by size:
4685 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 398 1044 3816 4261 5039 5229 6021 398
Size: 646 2772 445 778 190 792 1624
Fragments arranged by size:
2772 1624 792 778 646 445 190
BsmI GAATG_Cn'
Cuts at: 3117 3117
Size: 7247
BsmBI CGTCTCn'nnnn_
Cuts at: 237 485 2604 237
Size: 248 2119 4880
Fragments arranged by size:
4880 2119 248
Bsp1286I G_dGCh'C
Cuts at: 248 398 1044 2322 3424 3816 4058 4210 4261
Size: 150 646 1278 1102 392 242 152 51
Cuts at: 4261 4872 4965 5039 5229 5291 6021 248
Size: 611 93 74 190 62 730 1474
Fragments arranged by size:
1474 1278 1102 730 646 611 392 242
190 152 150 93 74 62 51
BspEI T'CCGG_A
Cuts at: 6467 6467
Size: 7247
BspHI T'CATG_A
Cuts at: 6423 6423
Size: 7247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1676 1683 4297 4511 4813 5194 1676
Size: 7 2614 214 302 381 3729
Fragments arranged by size:
3729 2614 381 302 214 7
BsrBI CCG'CTC
Cuts at: 50 5539 5593 7077 7242 50
Size: 5489 54 1484 165 55
Fragments arranged by size:
5489 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2622 2832 3427 4009 4179 5159 2622
Size: 210 595 582 170 980 4710
Fragments arranged by size:
4710 980 595 582 210 170
BsrFI r'CCGG_y
Cuts at: 275 5245 5426 5635 275
Size: 4970 181 209 1887
Fragments arranged by size:
4970 1887 209 181
BssSI C'ACGA_G
Cuts at: 2898 5518 5876 2898
Size: 2620 358 4269
Fragments arranged by size:
4269 2620 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3597 4624 3597
Size: 1027 6220
BstBI TT'CG_AA
Cuts at: 624 5608 624
Size: 4984 2263
BstXI CCAn_nnnn'nTGG
Cuts at: 732 3829 732
Size: 3097 4150
BstYI r'GATC_y
Cuts at: 1 370 858 1491 1938 2388 2435 3110 3192
Size: 369 488 633 447 450 47 675 82
Cuts at: 3192 3304 3832 4123 4272 4739 4762 5097 5343
Size: 112 528 291 149 467 23 335 246
Cuts at: 5343 6355 6441 6453 6799 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 675 633 528 488 467 450 449
447 369 346 335 291 246 149 112
86 82 47 23 12
BstZ17I GTA'TAC
Cuts at: 710 710
Size: 7247
BtgI C'CryG_G
Cuts at: 199 657 1817 2057 2872 2955 3577 199
Size: 458 1160 240 815 83 622 3869
Fragments arranged by size:
3869 1160 815 622 458 240 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5197 5378 5541 5197
Size: 181 163 6903
Fragments arranged by size:
6903 181 163
BtsI GCAGTG_nn'
Cuts at: 470 1403 1522 1616 3386 3554 4490 470
Size: 933 119 94 1770 168 936 3227
Fragments arranged by size:
3227 1770 936 933 168 119 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 513 548 513
Size: 35 7212
DraI TTT'AAA
Cuts at: 4246 4246
Size: 7247
DrdI GACnn_nn'nnGTC
Cuts at: 4953 5811 4953
Size: 858 6389
EaeI y'GGCC_r
Cuts at: 277 1063 2245 2890 2952 4089 4832 5006 5397
Size: 786 1182 645 62 1137 743 174 391
Cuts at: 5397 5424 6769 277
Size: 27 1345 755
Fragments arranged by size:
1345 1182 1137 786 755 743 645 391
174 62 27
EagI C'GGCC_G
Cuts at: 4832 4832
Size: 7247
EarI CTCTTCn'nnn_
Cuts at: 1448 2758 3285 3578 3773 5270 5480 1448
Size: 1310 527 293 195 1497 210 3215
Fragments arranged by size:
3215 1497 1310 527 293 210 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 283 4005 5455 5765 5911 7057 283
Size: 3722 1450 310 146 1146 473
Fragments arranged by size:
3722 1450 1146 473 310 146
EcoICRI GAG'CTC
Cuts at: 1042 4259 1042
Size: 3217 4030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 583 838 1247 1345 1623 1652 2117 2324
Size: 17 255 409 98 278 29 465 207
Cuts at: 2324 2384 3680 3743 4067 5072 5504 6251 6519
Size: 60 1296 63 324 1005 432 747 268
Cuts at: 6519 566
Size: 1294
Fragments arranged by size:
1296 1294 1005 747 465 432 409 324
278 268 255 207 98 63 60 29
17
EcoNI CCTnn'n_nnAGG
Cuts at: 419 2215 3509 419
Size: 1796 1294 4157
Fragments arranged by size:
4157 1796 1294
EcoO109I rG'GnC_Cy
Cuts at: 3492 3803 4386 3492
Size: 311 583 6353
Fragments arranged by size:
6353 583 311
EcoRI G'AATT_C
Cuts at: 4251 4251
Size: 7247
EcoRV GAT'ATC
Cuts at: 1959 1959
Size: 7247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5266 5266
Size: 7247
FseI GG_CCGG'CC
Cuts at: 279 279
Size: 7247
FspI TGC'GCA
Cuts at: 388 1948 5028 388
Size: 1560 3080 2607
Fragments arranged by size:
3080 2607 1560
FspAI rTGC'GCAy
Cuts at: 1948 1948
Size: 7247
HaeII r_GCGC'y
Cuts at: 4681 4929 5951 6930 4681
Size: 248 1022 979 4998
Fragments arranged by size:
4998 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 435 467 1068 1100 1625 1657 2067
Size: 32 75 32 601 32 525 32 410
Cuts at: 2067 2099 2109 2121 2141 2153 2266 2298 2427
Size: 32 10 12 20 12 113 32 129
Cuts at: 2427 2453 2459 2485 3936 3968 5089 5119 5121
Size: 26 6 26 1451 32 1121 30 2
Cuts at: 5121 5151 328
Size: 30 2424
Fragments arranged by size:
2424 1451 1121 601 525 410 129 113
75 32 32 32 32 32 32 32
30 30 26 26 20 12 12 10
6 2
HincII GTy'rAC
Cuts at: 2208 3623 4286 2208
Size: 1415 663 5169
Fragments arranged by size:
5169 1415 663
HindIII A'AGCT_T
Cuts at: 3785 3785
Size: 7247
KasI G'GCGC_C
Cuts at: 4677 4925 4677
Size: 248 6999
KpnI G_GTAC'C
Cuts at: 725 1745 4267 725
Size: 1020 2522 3705
Fragments arranged by size:
3705 2522 1020
MluI A'CGCG_T
Cuts at: 6680 6680
Size: 7247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 537 984 1283 2331 2553 3146 4235 5918 6102
Size: 447 299 1048 222 593 1089 1683 184
Cuts at: 6102 537
Size: 1682
Fragments arranged by size:
1683 1682 1089 1048 593 447 299 222
184
MscI TGG'CCA
Cuts at: 2892 4091 5008 2892
Size: 1199 917 5131
Fragments arranged by size:
5131 1199 917
MslI CAynn'nnrTG
Cuts at: 979 2154 2411 2668 3614 5236 5363 979
Size: 1175 257 257 946 1622 127 2863
Fragments arranged by size:
2863 1622 1175 946 257 257 127
MspA1I CmG'CkG
Cuts at: 201 368 544 1138 1808 4358 4672 5032 6045
Size: 167 176 594 670 2550 314 360 1013
Cuts at: 6045 6290 201
Size: 245 1158
Fragments arranged by size:
2550 1158 1013 670 594 360 314 245
176 167
NaeI GCC'GGC
Cuts at: 277 5428 277
Size: 5151 2096
NarI GG'CG_CC
Cuts at: 4678 4926 4678
Size: 248 6999
NcoI C'CATG_G
Cuts at: 2872 2872
Size: 7247
NdeI CA'TA_TG
Cuts at: 2740 3096 2740
Size: 356 6891
NgoMIV G'CCGG_C
Cuts at: 275 5426 275
Size: 5151 2096
NspI r_CATG'y
Cuts at: 4300 4800 5707 4300
Size: 500 907 5840
Fragments arranged by size:
5840 907 500
PciI A'CATG_T
Cuts at: 5703 5703
Size: 7247
PflMI CCAn_nnn'nTGG
Cuts at: 296 605 1392 2271 3459 296
Size: 309 787 879 1188 4084
Fragments arranged by size:
4084 1188 879 787 309
PfoI T'CCnGG_A
Cuts at: 560 1600 3828 560
Size: 1040 2228 3979
Fragments arranged by size:
3979 2228 1040
PmeI GTTT'AAAC
Cuts at: 4246 4246
Size: 7247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6386 6418 6386
Size: 32 7215
PpuMI rG'GwC_Cy
Cuts at: 3492 3492
Size: 7247
PshAI GACnn'nnGTC
Cuts at: 1925 3549 1925
Size: 1624 5623
PstI C_TGCA'G
Cuts at: 1529 2144 3605 4294 4979 1529
Size: 615 1461 689 685 3797
Fragments arranged by size:
3797 1461 689 685 615
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7247
PvuII CAG'CTG
Cuts at: 544 1138 1808 4672 5032 544
Size: 594 670 2864 360 2759
Fragments arranged by size:
2864 2759 670 594 360
RsrII CG'GwC_CG
Cuts at: 2498 5442 2498
Size: 2944 4303
SacI G_AGCT'C
Cuts at: 1044 4261 1044
Size: 3217 4030
SacII CC_GC'GG
Cuts at: 202 202
Size: 7247
SalI G'TCGA_C
Cuts at: 4284 4284
Size: 7247
SanDI GG'GwC_CC
Cuts at: 3492 3492
Size: 7247
SapI GCTCTTCn'nnn_
Cuts at: 5270 5480 5270
Size: 210 7037
SbfI CC_TGCA'GG
Cuts at: 4294 4294
Size: 7247
ScaI AGT'ACT
Cuts at: 6764 6764
Size: 7247
SexAI A'CCwGG_T
Cuts at: 806 806
Size: 7247
SfcI C'TryA_G
Cuts at: 33 830 1525 2140 3601 4059 4290 4975 5968
Size: 797 695 615 1461 458 231 685 993
Cuts at: 5968 6159 33
Size: 191 1121
Fragments arranged by size:
1461 1121 993 797 695 685 615 458
231 191
SfoI GGC'GCC
Cuts at: 4679 4927 4679
Size: 248 6999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7247
SmaI CCC'GGG
Cuts at: 580 4269 580
Size: 3689 3558
SmlI C'TyrA_G
Cuts at: 1085 4402 5317 5809 6071 1085
Size: 3317 915 492 262 2261
Fragments arranged by size:
3317 2261 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7247
SphI G_CATG'C
Cuts at: 4300 4800 4300
Size: 500 6747
StuI AGG'CCT
Cuts at: 3960 4048 3960
Size: 88 7159
StyI C'CwwG_G
Cuts at: 852 1908 2103 2265 2872 2985 3453 4049 4135
Size: 1056 195 162 607 113 468 596 86
Cuts at: 4135 4381 4735 852
Size: 246 354 3364
Fragments arranged by size:
3364 1056 607 596 468 354 246 195
162 113 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 602 2486 4830 4963 5422 5631 602
Size: 1884 2344 133 459 209 2218
Fragments arranged by size:
2344 2218 1884 459 209 133
TatI w'GTAC_w
Cuts at: 961 2340 3435 6762 961
Size: 1379 1095 3327 1446
Fragments arranged by size:
3327 1446 1379 1095
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1019 1051 1422 1454 6764 6796 1019
Size: 32 371 32 5310 32 1470
Fragments arranged by size:
5310 1470 371 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1626 5044 1626
Size: 3418 3829
XbaI T'CTAG_A
Cuts at: 3849 4278 3849
Size: 429 6818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 875 1142 1389 3352 4742 875
Size: 267 247 1963 1390 3380
Fragments arranged by size:
3380 1963 1390 267 247
XmaI C'CCGG_G
Cuts at: 578 4267 578
Size: 3689 3558
XmnI GAAnn'nnTTC
Cuts at: 4110 4110
Size: 7247
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstEII Bsu36I ClaI DraIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PmlI PsiI
PspOMI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI