Restriction map for
pF1KA1558
(Circular) (Six-base) MAPSORT of: pF1KA1558.seq Check: 929 from: 1 to: 5750
pF1KA1558 5750 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1774 1774
Size: 5750
AccI GT'mk_AC
Cuts at: 557 1252 2593 2788 557
Size: 695 1341 195 3519
Fragments arranged by size:
3519 1341 695 195
Acc65I G'GTAC_C
Cuts at: 2766 2766
Size: 5750
AclI AA'CG_TT
Cuts at: 5590 5590
Size: 5750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 462 2081 2711 3575 4007 4754 5022 199
Size: 263 1619 630 864 432 747 268 927
Fragments arranged by size:
1619 927 864 747 630 432 268 263
AflIII A'CryG_T
Cuts at: 619 1238 3102 4206 5183 619
Size: 619 1864 1104 977 1186
Fragments arranged by size:
1864 1186 1104 977 619
AgeI A'CCGG_T
Cuts at: 4138 4138
Size: 5750
AleI CACnn'nnGTG
Cuts at: 2728 2728
Size: 5750
AlwNI CAG_nnn'CTG
Cuts at: 2059 2542 3055 4622 2059
Size: 483 513 1567 3187
Fragments arranged by size:
3187 1567 513 483
ApaI G_GGCC'C
Cuts at: 1534 1534
Size: 5750
ApaLI G'TGCA_C
Cuts at: 1513 4520 1513
Size: 3007 2743
ApoI r'AATT_y
Cuts at: 118 791 1286 2754 5103 5359 118
Size: 673 495 1468 2349 256 509
Fragments arranged by size:
2349 1468 673 509 495 256
AseI AT'TA_AT
Cuts at: 20 1405 20
Size: 1385 4365
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5750
AvaI C'yCGr_G
Cuts at: 2025 2635 2686 2770 2025
Size: 610 51 84 5005
Fragments arranged by size:
5005 610 84 51
BamHI G'GATC_C
Cuts at: 1116 2775 1116
Size: 1659 4091
BanI G'GyrC_C
Cuts at: 2661 2766 3180 3428 3463 2661
Size: 105 414 248 35 4948
Fragments arranged by size:
4948 414 248 105 35
BanII G_rGCy'C
Cuts at: 812 1534 2764 3794 812
Size: 722 1230 1030 2768
Fragments arranged by size:
2768 1230 1030 722
BbeI G_GCGC'C
Cuts at: 2665 3184 3432 2665
Size: 519 248 4983
Fragments arranged by size:
4983 519 248
BbsI GAAGACnn'nnnn_
Cuts at: 1047 1102 1470 2282 2541 1047
Size: 55 368 812 259 4256
Fragments arranged by size:
4256 812 368 259 55
BbvCI CC'TCA_GC
Cuts at: 1454 1454
Size: 5750
BciVI GTATCCnnnnn_n'
Cuts at: 347 3648 4409 5750 347
Size: 3301 761 1341 347
Fragments arranged by size:
3301 1341 761 347
BclI T'GATC_A
Cuts at: 3270 3270
Size: 5750
BfrBI ATG'CAT
Cuts at: 739 739
Size: 5750
BglI GCCn_nnn'nGGC
Cuts at: 983 983
Size: 5750
BglII A'GATC_T
Cuts at: 971 1566 1647 3265 5302 971
Size: 595 81 1618 2037 1419
Fragments arranged by size:
2037 1618 1419 595 81
BlpI GC'TnA_GC
Cuts at: 2512 2862 2512
Size: 350 5400
Bme1580I G_kGCm'C
Cuts at: 1517 1534 3375 3468 4524 1517
Size: 17 1841 93 1056 2743
Fragments arranged by size:
2743 1841 1056 93 17
BmgBI CAC'GTC
Cuts at: 620 2288 620
Size: 1668 4082
BmrI ACTGGGnnnn_n'
Cuts at: 403 2369 2962 3043 3378 5522 403
Size: 1966 593 81 335 2144 631
Fragments arranged by size:
2144 1966 631 593 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2342 4117 2342
Size: 1775 3975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 2247 2312 1090
Size: 1157 65 4528
Fragments arranged by size:
4528 1157 65
Bpu10I CC'TnA_GC
Cuts at: 607 769 1454 2296 607
Size: 162 685 842 4061
Fragments arranged by size:
4061 842 685 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 955 2585 2926 3805 4297 4595 290
Size: 665 1630 341 879 492 298 1445
Fragments arranged by size:
1630 1445 879 665 492 341 298
BsaI GGTCTCn'nnnn_
Cuts at: 770 5454 770
Size: 4684 1066
BsaAI yAC'GTr
Cuts at: 3103 3103
Size: 5750
BsaBI GATnn'nnATC
Cuts at: 6 3264 5301 6
Size: 3258 2037 455
Fragments arranged by size:
3258 2037 455
BsaHI Gr'CG_yC
Cuts at: 1771 2662 3181 3429 4131 5627 1771
Size: 891 519 248 702 1496 1894
Fragments arranged by size:
1894 1496 891 702 519 248
BsaWI w'CCGG_w
Cuts at: 2939 3460 4138 4412 4559 4970 2939
Size: 521 678 274 147 411 3719
Fragments arranged by size:
3719 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 685 2251 2461 2641 685
Size: 1566 210 180 3794
Fragments arranged by size:
3794 1566 210 180
BseYI C'CCAG_C
Cuts at: 2098 2264 2674 3175 3933 4510 5006 2098
Size: 166 410 501 758 577 496 2842
Fragments arranged by size:
2842 758 577 501 496 410 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 1590 2416 1570
Size: 20 826 4904
Fragments arranged by size:
4904 826 20
BsiEI CG_ry'CG
Cuts at: 104 3292 3338 4546 104
Size: 3188 46 1208 1308
Fragments arranged by size:
3188 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1517 2199 2233 2302 2764 3542 3732 4524 1517
Size: 682 34 69 462 778 190 792 2743
Fragments arranged by size:
2743 792 778 682 462 190 69 34
BsmI GAATG_Cn'
Cuts at: 1993 1993
Size: 5750
BsmBI CGTCTCn'nnnn_
Cuts at: 1591 2251 1591
Size: 660 5090
Bsp1286I G_dGCh'C
Cuts at: 812 1517 1534 2199 2233 2302 2764 3375 3468
Size: 705 17 665 34 69 462 611 93
Cuts at: 3468 3542 3732 3794 4524 812
Size: 74 190 62 730 2038
Fragments arranged by size:
2038 730 705 665 611 462 190 93
74 69 62 34 17
BspEI T'CCGG_A
Cuts at: 4970 4970
Size: 5750
BspHI T'CATG_A
Cuts at: 1056 4926 1056
Size: 3870 1880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1669 2800 3014 3316 3697 1669
Size: 1131 214 302 381 3722
Fragments arranged by size:
3722 1131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4042 4096 5580 5745 50
Size: 3992 54 1484 165 55
Fragments arranged by size:
3992 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1842 3662 1842
Size: 1820 3930
BsrFI r'CCGG_y
Cuts at: 3748 3929 4138 3748
Size: 181 209 5360
Fragments arranged by size:
5360 209 181
BssSI C'ACGA_G
Cuts at: 4021 4379 4021
Size: 358 5392
BstAPI GCAn_nnn'nTGC
Cuts at: 651 3127 651
Size: 2476 3274
BstBI TT'CG_AA
Cuts at: 4111 4111
Size: 5750
BstXI CCAn_nnnn'nTGG
Cuts at: 2153 2153
Size: 5750
BstYI r'GATC_y
Cuts at: 1 971 1048 1116 1566 1647 2215 2775 3242
Size: 970 77 68 450 81 568 560 467
Cuts at: 3242 3265 3600 3846 4858 4944 4956 5302 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 970 568 560 467 450 449 346
335 246 86 81 77 68 23 12
BstZ17I GTA'TAC
Cuts at: 1253 1253
Size: 5750
Bsu36I CC'TnA_GG
Cuts at: 1617 1617
Size: 5750
BtgI C'CryG_G
Cuts at: 2190 2357 2423 2190
Size: 167 66 5517
Fragments arranged by size:
5517 167 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 695 1974 3700 3881 4044 695
Size: 1279 1726 181 163 2401
Fragments arranged by size:
2401 1726 1279 181 163
BtsI GCAGTG_nn'
Cuts at: 2109 2399 2421 2993 2109
Size: 290 22 572 4866
Fragments arranged by size:
4866 572 290 22
DraI TTT'AAA
Cuts at: 1701 2749 1701
Size: 1048 4702
DraIII CAC_nnn'GTG
Cuts at: 754 2719 754
Size: 1965 3785
DrdI GACnn_nn'nnGTC
Cuts at: 3456 4314 3456
Size: 858 4892
EaeI y'GGCC_r
Cuts at: 2420 2700 3335 3509 3900 3927 5272 2420
Size: 280 635 174 391 27 1345 2898
Fragments arranged by size:
2898 1345 635 391 280 174 27
EagI C'GGCC_G
Cuts at: 3335 3335
Size: 5750
EarI CTCTTCn'nnn_
Cuts at: 3773 3983 3773
Size: 210 5540
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3958 4268 4414 5560 3958
Size: 310 146 1146 4148
Fragments arranged by size:
4148 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2762 2762
Size: 5750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 462 1090 2081 2247 2312 2711 3575 4007
Size: 263 628 991 166 65 399 864 432
Cuts at: 4007 4754 5022 199
Size: 747 268 927
Fragments arranged by size:
991 927 864 747 628 432 399 268
263 166 65
EcoO109I rG'GnC_Cy
Cuts at: 1200 1530 1531 2889 1200
Size: 330 1 1358 4061
Fragments arranged by size:
4061 1358 330 1
EcoRI G'AATT_C
Cuts at: 2754 2754
Size: 5750
EcoRV GAT'ATC
Cuts at: 1160 1160
Size: 5750
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2103 3769 2103
Size: 1666 4084
FspI TGC'GCA
Cuts at: 3531 3531
Size: 5750
HaeII r_GCGC'y
Cuts at: 2665 3184 3432 4454 5433 2665
Size: 519 248 1022 979 2982
Fragments arranged by size:
2982 1022 979 519 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 265 297 1339 1371 1840 1872 1939
Size: 32 118 32 1042 32 469 32 67
Cuts at: 1939 1971 2207 2239 2272 2304 3592 3622 3624
Size: 32 236 32 33 32 1288 30 2
Cuts at: 3624 3654 115
Size: 30 2211
Fragments arranged by size:
2211 1288 1042 469 236 118 67 33
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2789 2789
Size: 5750
KasI G'GCGC_C
Cuts at: 2661 3180 3428 2661
Size: 519 248 4983
Fragments arranged by size:
4983 519 248
KpnI G_GTAC'C
Cuts at: 2770 2770
Size: 5750
MluI A'CGCG_T
Cuts at: 5183 5183
Size: 5750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4421 4605 4421
Size: 184 5566
MscI TGG'CCA
Cuts at: 2422 2702 3511 2422
Size: 280 809 4661
Fragments arranged by size:
4661 809 280
MslI CAynn'nnrTG
Cuts at: 734 2728 3739 3866 734
Size: 1994 1011 127 2618
Fragments arranged by size:
2618 1994 1011 127
MspA1I CmG'CkG
Cuts at: 2861 3175 3535 4548 4793 2861
Size: 314 360 1013 245 3818
Fragments arranged by size:
3818 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3931 3931
Size: 5750
NarI GG'CG_CC
Cuts at: 2662 3181 3429 2662
Size: 519 248 4983
Fragments arranged by size:
4983 519 248
NcoI C'CATG_G
Cuts at: 2357 2423 2357
Size: 66 5684
NgoMIV G'CCGG_C
Cuts at: 3929 3929
Size: 5750
NsiI A_TGCA'T
Cuts at: 741 741
Size: 5750
NspI r_CATG'y
Cuts at: 1242 1919 2803 3303 4210 1242
Size: 677 884 500 907 2782
Fragments arranged by size:
2782 907 884 677 500
PciI A'CATG_T
Cuts at: 1238 4206 1238
Size: 2968 2782
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 5750
PmeI GTTT'AAAC
Cuts at: 2749 2749
Size: 5750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 4889
Size: 32 5718
PpuMI rG'GwC_Cy
Cuts at: 1200 1200
Size: 5750
PshAI GACnn'nnGTC
Cuts at: 2545 2545
Size: 5750
PsiI TTA'TAA
Cuts at: 568 568
Size: 5750
PspOMI G'GGCC_C
Cuts at: 1530 1530
Size: 5750
PstI C_TGCA'G
Cuts at: 2646 2797 3482 2646
Size: 151 685 4914
Fragments arranged by size:
4914 685 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5750
PvuII CAG'CTG
Cuts at: 3175 3535 3175
Size: 360 5390
RsrII CG'GwC_CG
Cuts at: 3945 3945
Size: 5750
SacI G_AGCT'C
Cuts at: 2764 2764
Size: 5750
SalI G'TCGA_C
Cuts at: 2787 2787
Size: 5750
SapI GCTCTTCn'nnn_
Cuts at: 3773 3983 3773
Size: 210 5540
SbfI CC_TGCA'GG
Cuts at: 2797 2797
Size: 5750
ScaI AGT'ACT
Cuts at: 5267 5267
Size: 5750
SexAI A'CCwGG_T
Cuts at: 2715 2715
Size: 5750
SfcI C'TryA_G
Cuts at: 33 2522 2642 2741 2793 3478 4471 4662 33
Size: 2489 120 99 52 685 993 191 1121
Fragments arranged by size:
2489 1121 993 685 191 120 99 52
SfoI GGC'GCC
Cuts at: 2663 3182 3430 2663
Size: 519 248 4983
Fragments arranged by size:
4983 519 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5750
SmaI CCC'GGG
Cuts at: 2772 2772
Size: 5750
SmlI C'TyrA_G
Cuts at: 305 934 2600 2905 3820 4312 4574 305
Size: 629 1666 305 915 492 262 1481
Fragments arranged by size:
1666 1481 915 629 492 305 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5750
SphI G_CATG'C
Cuts at: 2803 3303 2803
Size: 500 5250
SspI AAT'ATT
Cuts at: 870 1899 870
Size: 1029 4721
StuI AGG'CCT
Cuts at: 2670 2670
Size: 5750
StyI C'CwwG_G
Cuts at: 2357 2423 2884 3238 2357
Size: 66 461 354 4869
Fragments arranged by size:
4869 461 354 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1092 2163 3333 3466 3925 4134 1092
Size: 1071 1170 133 459 209 2708
Fragments arranged by size:
2708 1170 1071 459 209 133
TatI w'GTAC_w
Cuts at: 802 2032 2050 2461 5265 802
Size: 1230 18 411 2804 1287
Fragments arranged by size:
2804 1287 1230 411 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 121 153 5267 5299 121
Size: 32 5114 32 572
Fragments arranged by size:
5114 572 32 32
Tth111I GACn'n_nGTC
Cuts at: 1579 3547 1579
Size: 1968 3782
XbaI T'CTAG_A
Cuts at: 156 831 1023 2781 156
Size: 675 192 1758 3125
Fragments arranged by size:
3125 1758 675 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3245 3245
Size: 5750
XmaI C'CCGG_G
Cuts at: 2770 2770
Size: 5750
XmnI GAAnn'nnTTC
Cuts at: 701 761 1240 701
Size: 60 479 5211
Fragments arranged by size:
5211 479 60
ZraI GAC'GTC
Cuts at: 1772 1772
Size: 5750
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BmtI BsaXI BsiWI BsrGI BssHII BstEII
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI