Restriction map for
pF1KA1549
(Circular) (Six-base) MAPSORT of: pF1KA1549.seq Check: 9118 from: 1 to: 8687
pF1KA1549 8687 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1821 3537 4993 1821
Size: 1716 1456 5515
Fragments arranged by size:
5515 1716 1456
AccI GT'mk_AC
Cuts at: 2077 2678 3731 4529 5203 5725 2077
Size: 601 1053 798 674 522 5039
Fragments arranged by size:
5039 1053 798 674 601 522
Acc65I G'GTAC_C
Cuts at: 5323 5703 5323
Size: 380 8307
AclI AA'CG_TT
Cuts at: 1701 4668 8527 1701
Size: 2967 3859 1861
Fragments arranged by size:
3859 2967 1861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1652 2456 2863 3013 3397 4042 5474 6512
Size: 1510 804 407 150 384 645 1432 1038
Cuts at: 6512 6944 7297 7959 142
Size: 432 353 662 870
Fragments arranged by size:
1510 1432 1038 870 804 662 645 432
407 384 353 150
AfeI AGC'GCT
Cuts at: 144 144
Size: 8687
AflIII A'CryG_T
Cuts at: 305 2342 2402 6039 7839 8120 305
Size: 2037 60 3637 1800 281 872
Fragments arranged by size:
3637 2037 1800 872 281 60
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 8687
AhdI GACnn_n'nnGTC
Cuts at: 1348 4536 1348
Size: 3188 5499
AleI CACnn'nnGTG
Cuts at: 850 2500 2524 2755 850
Size: 1650 24 231 6782
Fragments arranged by size:
6782 1650 231 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 161 691 1258 161
Size: 530 567 7590
Fragments arranged by size:
7590 567 530
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4653 4685 4653
Size: 32 8655
AlwNI CAG_nnn'CTG
Cuts at: 169 2179 3375 3912 3957 4126 5349 5992 7430
Size: 2010 1196 537 45 169 1223 643 1438
Cuts at: 7430 169
Size: 1426
Fragments arranged by size:
2010 1438 1426 1223 1196 643 537 169
45
ApaI G_GGCC'C
Cuts at: 2482 3205 5548 2482
Size: 723 2343 5621
Fragments arranged by size:
5621 2343 723
ApaLI G'TGCA_C
Cuts at: 7525 7525
Size: 8687
ApoI r'AATT_y
Cuts at: 624 1297 3704 5691 8040 8296 624
Size: 673 2407 1987 2349 256 1015
Fragments arranged by size:
2407 2349 1987 1015 673 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8687
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8687
AvaI C'yCGr_G
Cuts at: 1635 2059 4542 5707 1635
Size: 424 2483 1165 4615
Fragments arranged by size:
4615 2483 1165 424
BamHI G'GATC_C
Cuts at: 2419 4812 5712 2419
Size: 2393 900 5394
Fragments arranged by size:
5394 2393 900
BanI G'GyrC_C
Cuts at: 1219 2187 2502 4365 4756 5214 5323 5703 6117
Size: 968 315 1863 391 458 109 380 414
Cuts at: 6117 6365 6400 1219
Size: 248 35 3506
Fragments arranged by size:
3506 1863 968 458 414 391 380 315
248 109 35
BanII G_rGCy'C
Cuts at: 116 1111 2482 2605 3205 3341 4215 4646 4657
Size: 995 1371 123 600 136 874 431 11
Cuts at: 4657 5385 5504 5548 5636 5701 6731 116
Size: 728 119 44 88 65 1030 2072
Fragments arranged by size:
2072 1371 1030 995 874 728 600 431
136 123 119 88 65 44 11
BbeI G_GCGC'C
Cuts at: 6121 6369 6121
Size: 248 8439
BbsI GAAGACnn'nnnn_
Cuts at: 1670 2141 2276 3638 5141 5585 1670
Size: 471 135 1362 1503 444 4772
Fragments arranged by size:
4772 1503 1362 471 444 135
BbvCI CC'TCA_GC
Cuts at: 1166 2115 5228 5505 1166
Size: 949 3113 277 4348
Fragments arranged by size:
4348 3113 949 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1535 1569 4945 4979 1535
Size: 34 3376 34 5243
Fragments arranged by size:
5243 3376 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3045 3307 3359 4877 6585 7641 8687 3045
Size: 262 52 1518 1708 1056 1046 3045
Fragments arranged by size:
3045 1708 1518 1056 1046 262 52
BclI T'GATC_A
Cuts at: 2393 2747 6207 2393
Size: 354 3460 4873
Fragments arranged by size:
4873 3460 354
BglI GCCn_nnn'nGGC
Cuts at: 4972 5107 5436 4972
Size: 135 329 8223
Fragments arranged by size:
8223 329 135
BglII A'GATC_T
Cuts at: 3155 6202 8239 3155
Size: 3047 2037 3603
Fragments arranged by size:
3603 3047 2037
BlpI GC'TnA_GC
Cuts at: 3478 3947 5384 5799 3478
Size: 469 1437 415 6366
Fragments arranged by size:
6366 1437 469 415
Bme1580I G_kGCm'C
Cuts at: 140 2192 2482 3205 4511 4584 4628 5219 5548
Size: 2052 290 723 1306 73 44 591 329
Cuts at: 5548 6312 6405 7529 140
Size: 764 93 1124 1298
Fragments arranged by size:
2052 1306 1298 1124 764 723 591 329
290 93 73 44
BmgBI CAC'GTC
Cuts at: 1203 4056 1203
Size: 2853 5834
BmrI ACTGGGnnnn_n'
Cuts at: 143 1931 3313 3796 4232 4322 4867 4887 5070
Size: 1788 1382 483 436 90 545 20 183
Cuts at: 5070 5899 5980 6315 8459 143
Size: 829 81 335 2144 371
Fragments arranged by size:
2144 1788 1382 829 545 483 436 371
335 183 90 81 20
BmtI G_CTAG'C
Cuts at: 4316 4316
Size: 8687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3646 3767 4275 7054 3646
Size: 121 508 2779 5279
Fragments arranged by size:
5279 2779 508 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 1023 1323 1951 3412 3589 3658 5569 794
Size: 229 300 628 1461 177 69 1911 3912
Fragments arranged by size:
3912 1911 1461 628 300 229 177 69
Bpu10I CC'TnA_GC
Cuts at: 1105 1166 2115 5228 5505 1105
Size: 61 949 3113 277 4287
Fragments arranged by size:
4287 3113 949 277 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1972 2164 3165 3303 5863 6742 7456 7754
Size: 674 192 1001 138 2560 879 714 298
Cuts at: 7754 1298
Size: 2231
Fragments arranged by size:
2560 2231 1001 879 714 674 298 192
138
BsaI GGTCTCn'nnnn_
Cuts at: 804 1625 8391 804
Size: 821 6766 1100
Fragments arranged by size:
6766 1100 821
BsaAI yAC'GTr
Cuts at: 306 2343 4284 6040 306
Size: 2037 1941 1756 2953
Fragments arranged by size:
2953 2037 1941 1756
BsaBI GATnn'nnATC
Cuts at: 6 2741 3581 4591 4806 6201 8238 6
Size: 2735 840 1010 215 1395 2037 455
Fragments arranged by size:
2735 2037 1395 1010 840 455 215
BsaHI Gr'CG_yC
Cuts at: 1474 1525 2588 3410 4177 4778 4958 6118 6366
Size: 51 1063 822 767 601 180 1160 248
Cuts at: 6366 7068 8564 1474
Size: 702 1496 1597
Fragments arranged by size:
1597 1496 1160 1063 822 767 702 601
248 180 51
BsaWI w'CCGG_w
Cuts at: 2542 2887 3585 3753 5876 6397 7075 7486 7633
Size: 345 698 168 2123 521 678 411 147
Cuts at: 7633 7907 2542
Size: 274 3322
Fragments arranged by size:
3322 2123 698 678 521 411 345 274
168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 876 906 939 969 4279 4309 5278 5308 876
Size: 30 33 30 3310 30 969 30 4255
Fragments arranged by size:
4255 3310 969 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 962 2615 3182 4936 5299 5589 5626 5644
Size: 133 1653 567 1754 363 290 37 18
Cuts at: 5644 829
Size: 3872
Fragments arranged by size:
3872 1754 1653 567 363 290 133 37
18
BseYI C'CCAG_C
Cuts at: 182 283 3074 3470 3722 4389 4824 4907 4978
Size: 101 2791 396 252 667 435 83 71
Cuts at: 4978 5097 5553 6112 6870 7535 7943 182
Size: 119 456 559 758 665 408 926
Fragments arranged by size:
2791 926 758 667 665 559 456 435
408 396 252 119 101 83 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 854 1134 2165 2797 2852 3172 3565 3656
Size: 723 280 1031 632 55 320 393 91
Cuts at: 3656 4855 5686 131
Size: 1199 831 3132
Fragments arranged by size:
3132 1199 1031 831 723 632 393 320
280 91 55
BsiEI CG_ry'CG
Cuts at: 104 1458 4398 6229 6275 7505 104
Size: 1354 2940 1831 46 1230 1286
Fragments arranged by size:
2940 1831 1354 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 116 2331 2533 4215 4283 4469 4657 5385 5636
Size: 2215 202 1682 68 186 188 728 251
Cuts at: 5636 5662 5701 6479 6669 7529 116
Size: 26 39 778 190 860 1274
Fragments arranged by size:
2215 1682 1274 860 778 728 251 202
190 188 186 68 39 26
BsiWI C'GTAC_G
Cuts at: 5444 5444
Size: 8687
BsmI GAATG_Cn'
Cuts at: 937 1568 937
Size: 631 8056
BsmBI CGTCTCn'nnnn_
Cuts at: 1378 1823 3755 4046 1378
Size: 445 1932 291 6019
Fragments arranged by size:
6019 1932 445 291
Bsp1286I G_dGCh'C
Cuts at: 116 140 1111 2192 2331 2482 2533 2605 3205
Size: 24 971 1081 139 151 51 72 600
Cuts at: 3205 3341 4215 4283 4469 4511 4584 4628 4646
Size: 136 874 68 186 42 73 44 18
Cuts at: 4646 4657 5219 5385 5504 5548 5636 5662 5701
Size: 11 562 166 119 44 88 26 39
Cuts at: 5701 6312 6405 6479 6669 6731 7529 116
Size: 611 93 74 190 62 798 1274
Fragments arranged by size:
1274 1081 971 874 798 611 600 562
190 186 166 151 139 136 119 93
88 74 73 72 68 62 51 44
44 42 39 26 24 18 11
BspEI T'CCGG_A
Cuts at: 3753 3753
Size: 8687
BspHI T'CATG_A
Cuts at: 3993 7119 3993
Size: 3126 5561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1821 2417 3537 4190 4993 5737 5951 6253 6634
Size: 596 1120 653 803 744 214 302 381
Cuts at: 6634 1821
Size: 3874
Fragments arranged by size:
3874 1120 803 744 653 596 381 302
214
BsrBI CCG'CTC
Cuts at: 50 4223 6979 7033 8517 8682 50
Size: 4173 2756 54 1484 165 55
Fragments arranged by size:
4173 2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 958 3716 5164 6599 958
Size: 2758 1448 1435 3046
Fragments arranged by size:
3046 2758 1448 1435
BsrFI r'CCGG_y
Cuts at: 1470 2542 4316 4730 6685 6866 1470
Size: 1072 1774 414 1955 181 3291
Fragments arranged by size:
3291 1955 1774 1072 414 181
BssHII G'CGCG_C
Cuts at: 4491 4491
Size: 8687
BssSI C'ACGA_G
Cuts at: 115 1182 4969 6958 7666 115
Size: 1067 3787 1989 708 1136
Fragments arranged by size:
3787 1989 1136 1067 708
BstAPI GCAn_nnn'nTGC
Cuts at: 934 2179 6064 934
Size: 1245 3885 3557
Fragments arranged by size:
3885 3557 1245
BstBI TT'CG_AA
Cuts at: 7048 7048
Size: 8687
BstEII G'GTnAC_C
Cuts at: 3697 5557 3697
Size: 1860 6827
BstXI CCAn_nnnn'nTGG
Cuts at: 1136 2580 3693 1136
Size: 1444 1113 6130
Fragments arranged by size:
6130 1444 1113
BstYI r'GATC_y
Cuts at: 1 279 1308 1366 2419 3155 3917 4076 4292
Size: 278 1029 58 1053 736 762 159 216
Cuts at: 4292 4334 4481 4601 4812 5273 5339 5712 6179
Size: 42 147 120 211 461 66 373 467
Cuts at: 6179 6202 6537 6783 7089 7101 7187 8239 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1053 1052 1029 762 736 467 461 449
373 335 306 278 246 216 211 159
147 120 86 66 58 42 23 12
BtgI C'CryG_G
Cuts at: 107 385 476 1370 1452 2032 2519 4503 4613
Size: 278 91 894 82 580 487 1984 110
Cuts at: 4613 5573 107
Size: 960 3221
Fragments arranged by size:
3221 1984 960 894 580 487 278 110
91 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 905 4456 4766 6637 6818 6981 905
Size: 3551 310 1871 181 163 2611
Fragments arranged by size:
3551 2611 1871 310 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 2177 2840 4006 4231 5930 1541
Size: 636 663 1166 225 1699 4298
Fragments arranged by size:
4298 1699 1166 663 636 225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 213 248 1932 1967 213
Size: 35 1684 35 6933
Fragments arranged by size:
6933 1684 35 35
DraI TTT'AAA
Cuts at: 1991 5686 1991
Size: 3695 4992
DraIII CAC_nnn'GTG
Cuts at: 1139 4569 5078 1139
Size: 3430 509 4748
Fragments arranged by size:
4748 3430 509
DrdI GACnn_nn'nnGTC
Cuts at: 4135 6393 7737 4135
Size: 2258 1344 5085
Fragments arranged by size:
5085 2258 1344
EaeI y'GGCC_r
Cuts at: 329 1178 1455 2762 3887 4616 5108 5592 6272
Size: 849 277 1307 1125 729 492 484 680
Cuts at: 6272 6446 6837 6864 8209 329
Size: 174 391 27 1345 807
Fragments arranged by size:
1345 1307 1125 849 807 729 680 492
484 391 277 174 27
EagI C'GGCC_G
Cuts at: 1455 6272 1455
Size: 4817 3870
EarI CTCTTCn'nnn_
Cuts at: 1800 1937 2763 3736 4101 4139 4645 5054 5463
Size: 137 826 973 365 38 506 409 409
Cuts at: 5463 6710 6920 1800
Size: 1247 210 3567
Fragments arranged by size:
3567 1247 973 826 506 409 409 365
210 137 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 543 3516 4420 4474 5235 6895 7637 7783 8497
Size: 2973 904 54 761 1660 742 146 714
Cuts at: 8497 543
Size: 733
Fragments arranged by size:
2973 1660 904 761 742 733 714 146
54
EcoICRI GAG'CTC
Cuts at: 114 4213 4655 5383 5634 5699 114
Size: 4099 442 728 251 65 3102
Fragments arranged by size:
4099 3102 728 442 251 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 794 1023 1323 1652 1951 2456 2863 3013
Size: 652 229 300 329 299 505 407 150
Cuts at: 3013 3397 3412 3589 3658 4042 5474 5569 6512
Size: 384 15 177 69 384 1432 95 943
Cuts at: 6512 6944 7297 7959 142
Size: 432 353 662 870
Fragments arranged by size:
1432 943 870 662 652 505 432 407
384 384 353 329 300 299 229 177
150 95 69 15
EcoNI CCTnn'n_nnAGG
Cuts at: 700 4764 5353 700
Size: 4064 589 4034
Fragments arranged by size:
4064 4034 589
EcoO109I rG'GnC_Cy
Cuts at: 3201 4015 4202 4323 4769 5011 5084 5544 5545
Size: 814 187 121 446 242 73 460 1
Cuts at: 5545 5826 3201
Size: 281 6062
Fragments arranged by size:
6062 814 460 446 281 242 187 121
73 1
EcoRI G'AATT_C
Cuts at: 3704 5691 3704
Size: 1987 6700
EcoRV GAT'ATC
Cuts at: 2724 2724
Size: 8687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2063 3885 6706 2063
Size: 1822 2821 4044
Fragments arranged by size:
4044 2821 1822
FspI TGC'GCA
Cuts at: 6468 6468
Size: 8687
HaeII r_GCGC'y
Cuts at: 146 4006 4383 4503 6121 6369 7599 8370 146
Size: 3860 377 120 1618 248 1230 771 463
Fragments arranged by size:
3860 1618 1230 771 463 377 248 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 381 413 939 971 997 1029 1192 1224 1334
Size: 32 526 32 26 32 163 32 110
Cuts at: 1334 1366 1732 1764 1900 1932 3671 3703 4114
Size: 32 366 32 136 32 1739 32 411
Cuts at: 4114 4146 4186 4218 4279 4311 4522 4525 4554
Size: 32 40 32 61 32 211 3 29
Cuts at: 4554 4557 5278 5310 6529 6559 6561 6591 381
Size: 3 721 32 1219 30 2 30 2477
Fragments arranged by size:
2477 1739 1219 721 526 411 366 211
163 136 110 61 40 32 32 32
32 32 32 32 32 32 32 32
32 30 30 29 26 3 3 2
HincII GTy'rAC
Cuts at: 2679 3732 4191 4701 5726 2679
Size: 1053 459 510 1025 5640
Fragments arranged by size:
5640 1053 1025 510 459
HindIII A'AGCT_T
Cuts at: 2040 2941 2040
Size: 901 7786
KasI G'GCGC_C
Cuts at: 6117 6365 6117
Size: 248 8439
KpnI G_GTAC'C
Cuts at: 5327 5707 5327
Size: 380 8307
MluI A'CGCG_T
Cuts at: 2402 8120 2402
Size: 5718 2969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 634 3236 7446 7630 634
Size: 2602 4210 184 1691
Fragments arranged by size:
4210 2602 1691 184
MscI TGG'CCA
Cuts at: 1180 2764 3889 5594 6448 1180
Size: 1584 1125 1705 854 3419
Fragments arranged by size:
3419 1705 1584 1125 854
MslI CAynn'nnrTG
Cuts at: 292 850 1240 1999 2500 2524 2578 2755 2923
Size: 558 390 759 501 24 54 177 168
Cuts at: 2923 3802 6676 6803 292
Size: 879 2874 127 2176
Fragments arranged by size:
2874 2176 879 759 558 501 390 177
168 127 54 24
MspA1I CmG'CkG
Cuts at: 538 1773 1784 1926 3474 4505 5798 6112 6472
Size: 1235 11 142 1548 1031 1293 314 360
Cuts at: 6472 7256 7501 538
Size: 784 245 1724
Fragments arranged by size:
1724 1548 1293 1235 1031 784 360 314
245 142 11
NaeI GCC'GGC
Cuts at: 1472 4318 6868 1472
Size: 2846 2550 3291
Fragments arranged by size:
3291 2846 2550
NarI GG'CG_CC
Cuts at: 6118 6366 6118
Size: 248 8439
NcoI C'CATG_G
Cuts at: 107 476 1370 2032 107
Size: 369 894 662 6762
Fragments arranged by size:
6762 894 662 369
NdeI CA'TA_TG
Cuts at: 543 2712 543
Size: 2169 6518
NgoMIV G'CCGG_C
Cuts at: 1470 4316 6866 1470
Size: 2846 2550 3291
Fragments arranged by size:
3291 2846 2550
NheI G'CTAG_C
Cuts at: 4312 4312
Size: 8687
NspI r_CATG'y
Cuts at: 5740 6240 7843 5740
Size: 500 1603 6584
Fragments arranged by size:
6584 1603 500
PasI CC'CwG_GG
Cuts at: 437 5548 437
Size: 5111 3576
PciI A'CATG_T
Cuts at: 7839 7839
Size: 8687
PflMI CCAn_nnn'nTGG
Cuts at: 616 5078 5182 616
Size: 4462 104 4121
Fragments arranged by size:
4462 4121 104
PfoI T'CCnGG_A
Cuts at: 4604 4949 5515 4604
Size: 345 566 7776
Fragments arranged by size:
7776 566 345
PmeI GTTT'AAAC
Cuts at: 5686 5686
Size: 8687
PmlI CAC'GTG
Cuts at: 2343 4284 2343
Size: 1941 6746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 443 475 2992 3024 7133 7165 443
Size: 32 2517 32 4109 32 1965
Fragments arranged by size:
4109 2517 1965 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4015 4202 4323 5084 4015
Size: 187 121 761 7618
Fragments arranged by size:
7618 761 187 121
PshAI GACnn'nnGTC
Cuts at: 2254 2254
Size: 8687
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 8687
PspOMI G'GGCC_C
Cuts at: 2478 3201 5544 2478
Size: 723 2343 5621
Fragments arranged by size:
5621 2343 723
PstI C_TGCA'G
Cuts at: 1916 3329 3531 3917 5125 5201 5543 5734 6419
Size: 1413 202 386 1208 76 342 191 685
Cuts at: 6419 1916
Size: 4184
Fragments arranged by size:
4184 1413 1208 685 386 342 202 191
76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8687
PvuII CAG'CTG
Cuts at: 1926 3474 6112 6472 1926
Size: 1548 2638 360 4141
Fragments arranged by size:
4141 2638 1548 360
RsrII CG'GwC_CG
Cuts at: 6882 6882
Size: 8687
SacI G_AGCT'C
Cuts at: 116 4215 4657 5385 5636 5701 116
Size: 4099 442 728 251 65 3102
Fragments arranged by size:
4099 3102 728 442 251 65
SacII CC_GC'GG
Cuts at: 4506 4506
Size: 8687
SalI G'TCGA_C
Cuts at: 2677 3730 5724 2677
Size: 1053 1994 5640
Fragments arranged by size:
5640 1994 1053
SanDI GG'GwC_CC
Cuts at: 4323 4323
Size: 8687
SapI GCTCTTCn'nnn_
Cuts at: 2763 4645 6710 6920 2763
Size: 1882 2065 210 4530
Fragments arranged by size:
4530 2065 1882 210
SbfI CC_TGCA'GG
Cuts at: 5734 5734
Size: 8687
ScaI AGT'ACT
Cuts at: 2810 3026 8204 2810
Size: 216 5178 3293
Fragments arranged by size:
5178 3293 216
SexAI A'CCwGG_T
Cuts at: 4694 5175 4694
Size: 481 8206
SfcI C'TryA_G
Cuts at: 33 1912 3325 3521 3527 3913 4761 5091 5121
Size: 1879 1413 196 6 386 848 330 30
Cuts at: 5121 5197 5204 5539 5730 6415 7383 7574 33
Size: 76 7 335 191 685 968 191 1146
Fragments arranged by size:
1879 1413 1146 968 848 685 386 335
330 196 191 191 76 30 7 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 5436 5436
Size: 8687
SfoI GGC'GCC
Cuts at: 6119 6367 6119
Size: 248 8439
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8687
SgrAI Cr'CCGG_yG
Cuts at: 2542 2542
Size: 8687
SmaI CCC'GGG
Cuts at: 5709 5709
Size: 8687
SmlI C'TyrA_G
Cuts at: 1313 1951 2059 2143 3180 3282 5842 6757 7471
Size: 638 108 84 1037 102 2560 915 714
Cuts at: 7471 7733 1313
Size: 262 2267
Fragments arranged by size:
2560 2267 1037 915 714 638 262 108
102 84
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8687
SphI G_CATG'C
Cuts at: 5740 6240 5740
Size: 500 8187
StuI AGG'CCT
Cuts at: 3092 5242 3092
Size: 2150 6537
StyI C'CwwG_G
Cuts at: 107 252 476 788 1370 1874 2032 5482 5821
Size: 145 224 312 582 504 158 3450 339
Cuts at: 5821 6175 107
Size: 354 2619
Fragments arranged by size:
3450 2619 582 504 354 339 312 224
158 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 566 1545 2508 2572 3088 4412 5089 6270 6403
Size: 979 963 64 516 1324 677 1181 133
Cuts at: 6403 6862 7071 566
Size: 459 209 2182
Fragments arranged by size:
2182 1324 1181 979 963 677 516 459
209 133 64
TatI w'GTAC_w
Cuts at: 271 2783 2808 3024 4895 8202 271
Size: 2512 25 216 1871 3307 756
Fragments arranged by size:
3307 2512 1871 756 216 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1425 1457 2544 2576 3305 3337 4358 4390 8204
Size: 32 1087 32 729 32 1021 32 3814
Cuts at: 8204 8236 1425
Size: 32 1876
Fragments arranged by size:
3814 1876 1087 1021 729 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 6484 6484
Size: 8687
XbaI T'CTAG_A
Cuts at: 1363 2331 5718 1363
Size: 968 3387 4332
Fragments arranged by size:
4332 3387 968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 291 371 387 818 1188 6182 291
Size: 80 16 431 370 4994 2796
Fragments arranged by size:
4994 2796 431 370 80 16
XhoI C'TCGA_G
Cuts at: 2059 2059
Size: 8687
XmaI C'CCGG_G
Cuts at: 5707 5707
Size: 8687
XmnI GAAnn'nnTTC
Cuts at: 3440 3934 4110 3440
Size: 494 176 8017
Fragments arranged by size:
8017 494 176
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BfrBI BsrGI BstZ17I
Bsu36I ClaI FseI FspAI HpaI MfeI NotI NruI
NsiI PacI PspXI PsrI SnaBI SrfI SspI SwaI
ZraI