Restriction map for
pF1KA1539
(Circular) (Six-base) MAPSORT of: pF1KA1539.seq Check: 938 from: 1 to: 4727
pF1KA1539 4727 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 980 980
Size: 4727
AccI GT'mk_AC
Cuts at: 248 1765 248
Size: 1517 3210
Acc65I G'GTAC_C
Cuts at: 280 1743 280
Size: 1463 3264
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 407 503 1075 2552 2984 3337 3999 364
Size: 43 96 572 1477 432 353 662 1092
Fragments arranged by size:
1477 1092 662 572 432 353 96 43
AflII C'TTAA_G
Cuts at: 1596 1596
Size: 4727
AflIII A'CryG_T
Cuts at: 2079 3879 4160 2079
Size: 1800 281 2646
Fragments arranged by size:
2646 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 701 586
Size: 115 4612
AlwNI CAG_nnn'CTG
Cuts at: 584 715 2032 3470 584
Size: 131 1317 1438 1841
Fragments arranged by size:
1841 1438 1317 131
ApaI G_GGCC'C
Cuts at: 189 303 986 189
Size: 114 683 3930
Fragments arranged by size:
3930 683 114
ApaLI G'TGCA_C
Cuts at: 3565 3565
Size: 4727
ApoI r'AATT_y
Cuts at: 1731 4080 4336 1731
Size: 2349 256 2122
Fragments arranged by size:
2349 2122 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 241 335 781 1747 241
Size: 94 446 966 3221
Fragments arranged by size:
3221 966 446 94
AvrII C'CTAG_G
Cuts at: 745 745
Size: 4727
BamHI G'GATC_C
Cuts at: 907 1752 907
Size: 845 3882
BanI G'GyrC_C
Cuts at: 280 899 1381 1503 1743 2157 2405 2440 280
Size: 619 482 122 240 414 248 35 2567
Fragments arranged by size:
2567 619 482 414 248 240 122 35
BanII G_rGCy'C
Cuts at: 189 303 410 709 986 1047 1066 1105 1582
Size: 114 107 299 277 61 19 39 477
Cuts at: 1582 1661 1741 2771 189
Size: 79 80 1030 2145
Fragments arranged by size:
2145 1030 477 299 277 114 107 80
79 61 39 19
BbeI G_GCGC'C
Cuts at: 903 2161 2409 903
Size: 1258 248 3221
Fragments arranged by size:
3221 1258 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4693
BciVI GTATCCnnnnn_n'
Cuts at: 267 2625 3681 4727 267
Size: 2358 1056 1046 267
Fragments arranged by size:
2358 1056 1046 267
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1060 1099 1839 1060
Size: 39 740 3948
Fragments arranged by size:
3948 740 39
Bme1580I G_kGCm'C
Cuts at: 189 303 776 986 1373 1384 2352 2445 3569
Size: 114 473 210 387 11 968 93 1124
Cuts at: 3569 189
Size: 1347
Fragments arranged by size:
1347 1124 968 473 387 210 114 93
11
BmgBI CAC'GTC
Cuts at: 1260 1260
Size: 4727
BmrI ACTGGGnnnn_n'
Cuts at: 594 703 1103 1939 2020 2355 4499 594
Size: 109 400 836 81 335 2144 822
Fragments arranged by size:
2144 836 822 400 335 109 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 4727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 175 549 629 869 984 1663 155
Size: 20 374 80 240 115 679 3219
Fragments arranged by size:
3219 679 374 240 115 80 20
Bpu10I CC'TnA_GC
Cuts at: 1587 1587
Size: 4727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 2782 3496 3794 1903
Size: 879 714 298 2836
Fragments arranged by size:
2836 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4431 4431
Size: 4727
BsaAI yAC'GTr
Cuts at: 117 2080 117
Size: 1963 2764
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 900 2158 2406 3108 4604 900
Size: 1258 248 702 1496 1023
Fragments arranged by size:
1496 1258 1023 702 248
BsaWI w'CCGG_w
Cuts at: 1079 1574 1916 2437 3115 3526 3673 3947 1079
Size: 495 342 521 678 411 147 274 1859
Fragments arranged by size:
1859 678 521 495 411 342 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 574 604 1344 1374 574
Size: 30 740 30 3927
Fragments arranged by size:
3927 740 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1531 1537 1531
Size: 6 4721
BseYI C'CCAG_C
Cuts at: 219 360 432 715 1152 1253 2152 2910 3575
Size: 141 72 283 437 101 899 758 665
Cuts at: 3575 3983 219
Size: 408 963
Fragments arranged by size:
963 899 758 665 437 408 283 141
101 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 640 1546 139
Size: 501 906 3320
Fragments arranged by size:
3320 906 501
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3545 104
Size: 2165 46 1230 1286
Fragments arranged by size:
2165 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 788 1582 1741 2519 2709 3569 788
Size: 794 159 778 190 860 1946
Fragments arranged by size:
1946 860 794 778 190 159
BsmBI CGTCTCn'nnnn_
Cuts at: 137 137
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 189 303 410 709 776 788 986 1047 1066
Size: 114 107 299 67 12 198 61 19
Cuts at: 1066 1105 1373 1384 1582 1661 1741 2352 2445
Size: 39 268 11 198 79 80 611 93
Cuts at: 2445 2519 2709 2771 3569 189
Size: 74 190 62 798 1347
Fragments arranged by size:
1347 798 611 299 268 198 198 190
114 107 93 80 79 74 67 62
61 39 19 12 11
BspEI T'CCGG_A
Cuts at: 1079 1574 1079
Size: 495 4232
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 980 1777 1991 2293 2674 980
Size: 797 214 302 381 3033
Fragments arranged by size:
3033 797 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1586 3019 3073 4557 4722 50
Size: 1536 1433 54 1484 165 55
Fragments arranged by size:
1536 1484 1433 165 55 54
BsrDI GCAATG_nn'
Cuts at: 732 1171 2639 732
Size: 439 1468 2820
Fragments arranged by size:
2820 1468 439
BsrFI r'CCGG_y
Cuts at: 2725 2906 2725
Size: 181 4546
BssSI C'ACGA_G
Cuts at: 2998 3706 2998
Size: 708 4019
BstAPI GCAn_nnn'nTGC
Cuts at: 2104 2104
Size: 4727
BstBI TT'CG_AA
Cuts at: 1071 3088 1071
Size: 2017 2710
BstYI r'GATC_y
Cuts at: 1 597 907 1335 1752 2219 2242 2577 2823
Size: 596 310 428 417 467 23 335 246
Cuts at: 2823 3129 3141 3227 4279 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 596 467 449 428 417 335 310
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1567 1567
Size: 4727
BtgI C'CryG_G
Cuts at: 468 1022 468
Size: 554 4173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1474 2677 2858 3021 1474
Size: 1203 181 163 3180
Fragments arranged by size:
3180 1203 181 163
BtsI GCAGTG_nn'
Cuts at: 761 1970 761
Size: 1209 3518
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3777 2433
Size: 1344 3383
EaeI y'GGCC_r
Cuts at: 446 1200 2312 2486 2877 2904 4249 446
Size: 754 1112 174 391 27 1345 924
Fragments arranged by size:
1345 1112 924 754 391 174 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 340 872 2750 2960 340
Size: 532 1878 210 2107
Fragments arranged by size:
2107 1878 532 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 1044 1606 2935 3677 3823 4537 139
Size: 905 562 1329 742 146 714 329
Fragments arranged by size:
1329 905 742 714 562 329 146
EcoICRI GAG'CTC
Cuts at: 1580 1739 1580
Size: 159 4568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 175 364 407 503 549 629 869 984
Size: 20 189 43 96 46 80 240 115
Cuts at: 984 1075 1663 2552 2984 3337 3999 155
Size: 91 588 889 432 353 662 883
Fragments arranged by size:
889 883 662 588 432 353 240 189
115 96 91 80 46 43 20
EcoNI CCTnn'n_nnAGG
Cuts at: 1081 1350 1081
Size: 269 4458
EcoO109I rG'GnC_Cy
Cuts at: 185 186 299 300 331 352 524 614 814
Size: 1 113 1 31 21 172 90 200
Cuts at: 814 1689 1866 185
Size: 875 177 3046
Fragments arranged by size:
3046 875 200 177 172 113 90 31
21 1 1
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
EcoRV GAT'ATC
Cuts at: 1614 1614
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 903 2161 2409 3639 4410 903
Size: 1258 248 1230 771 1220
Fragments arranged by size:
1258 1230 1220 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 853 885 2569 2599 2601 2631 574
Size: 32 247 32 1684 30 2 30 2670
Fragments arranged by size:
2670 1684 247 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1766 1766
Size: 4727
KasI G'GCGC_C
Cuts at: 899 2157 2405 899
Size: 1258 248 3221
Fragments arranged by size:
3221 1258 248
KpnI G_GTAC'C
Cuts at: 284 1747 284
Size: 1463 3264
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3486 3670 3486
Size: 184 4543
MscI TGG'CCA
Cuts at: 448 1202 2488 448
Size: 754 1286 2687
Fragments arranged by size:
2687 1286 754
MslI CAynn'nnrTG
Cuts at: 2716 2843 2716
Size: 127 4600
MspA1I CmG'CkG
Cuts at: 654 1838 2152 2512 3296 3541 654
Size: 1184 314 360 784 245 1840
Fragments arranged by size:
1840 1184 784 360 314 245
NaeI GCC'GGC
Cuts at: 2908 2908
Size: 4727
NarI GG'CG_CC
Cuts at: 900 2158 2406 900
Size: 1258 248 3221
Fragments arranged by size:
3221 1258 248
NcoI C'CATG_G
Cuts at: 1022 1022
Size: 4727
NgoMIV G'CCGG_C
Cuts at: 2906 2906
Size: 4727
NspI r_CATG'y
Cuts at: 1461 1780 2280 3883 1461
Size: 319 500 1603 2305
Fragments arranged by size:
2305 1603 500 319
PasI CC'CwG_GG
Cuts at: 355 618 631 912 355
Size: 263 13 281 4170
Fragments arranged by size:
4170 281 263 13
PciI A'CATG_T
Cuts at: 3879 3879
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 584 715 1419 584
Size: 131 704 3892
Fragments arranged by size:
3892 704 131
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PmlI CAC'GTG
Cuts at: 117 117
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 331 614 331
Size: 283 4444
PshAI GACnn'nnGTC
Cuts at: 612 1016 612
Size: 404 4323
PspOMI G'GGCC_C
Cuts at: 185 299 982 185
Size: 114 683 3930
Fragments arranged by size:
3930 683 114
PstI C_TGCA'G
Cuts at: 1676 1774 2459 1676
Size: 98 685 3944
Fragments arranged by size:
3944 685 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 654 2152 2512 654
Size: 1498 360 2869
Fragments arranged by size:
2869 1498 360
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1582 1741 1582
Size: 159 4568
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SanDI GG'GwC_CC
Cuts at: 331 614 331
Size: 283 4444
SapI GCTCTTCn'nnn_
Cuts at: 2750 2960 2750
Size: 210 4517
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SexAI A'CCwGG_T
Cuts at: 251 251
Size: 4727
SfcI C'TryA_G
Cuts at: 33 213 1672 1770 2455 3423 3614 33
Size: 180 1459 98 685 968 191 1146
Fragments arranged by size:
1459 1146 968 685 191 180 98
SfoI GGC'GCC
Cuts at: 901 2159 2407 901
Size: 1258 248 3221
Fragments arranged by size:
3221 1258 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 243 337 1749 243
Size: 94 1412 3221
Fragments arranged by size:
3221 1412 94
SmlI C'TyrA_G
Cuts at: 1596 1882 2797 3511 3773 1596
Size: 286 915 714 262 2550
Fragments arranged by size:
2550 915 714 286 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
StuI AGG'CCT
Cuts at: 1094 1094
Size: 4727
StyI C'CwwG_G
Cuts at: 745 1022 1346 1373 1861 2215 745
Size: 277 324 27 488 354 3257
Fragments arranged by size:
3257 488 354 324 277 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1501 1552 1699 2310 2443 2902 3111 1501
Size: 51 147 611 133 459 209 3117
Fragments arranged by size:
3117 611 459 209 147 133 51
TatI w'GTAC_w
Cuts at: 4242 4242
Size: 4727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 315 347 428 460 4244 4276 315
Size: 32 81 32 3784 32 766
Fragments arranged by size:
3784 766 81 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 976 1416 2222 976
Size: 440 806 3481
Fragments arranged by size:
3481 806 440
XmaI C'CCGG_G
Cuts at: 241 335 1747 241
Size: 94 1412 3221
Fragments arranged by size:
3221 1412 94
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AloI AscI BaeI
BbsI BbvCI BfrBI BglI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI