Restriction map for
pF1KA1526
(Circular) (Six-base) MAPSORT of: pF1KA1526.seq Check: 9909 from: 1 to: 5834
pF1KA1526 5834 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1412 1412
Size: 5834
AatII G_ACGT'C
Cuts at: 1184 2312 1184
Size: 1128 4706
AccI GT'mk_AC
Cuts at: 2872 2872
Size: 5834
Acc65I G'GTAC_C
Cuts at: 2850 2850
Size: 5834
AclI AA'CG_TT
Cuts at: 5674 5674
Size: 5834
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 751 1067 3659 4091 4444 5106 552
Size: 199 316 2592 432 353 662 1280
Fragments arranged by size:
2592 1280 662 432 353 316 199
AfeI AGC'GCT
Cuts at: 242 242
Size: 5834
AflII C'TTAA_G
Cuts at: 1144 1144
Size: 5834
AflIII A'CryG_T
Cuts at: 608 3186 4986 5267 608
Size: 2578 1800 281 1175
Fragments arranged by size:
2578 1800 1175 281
AhdI GACnn_n'nnGTC
Cuts at: 129 655 969 129
Size: 526 314 4994
Fragments arranged by size:
4994 526 314
AleI CACnn'nnGTG
Cuts at: 814 872 1030 1210 2669 814
Size: 58 158 180 1459 3979
Fragments arranged by size:
3979 1459 180 158 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2500 2500
Size: 5834
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1221 1253 1221
Size: 32 5802
AlwNI CAG_nnn'CTG
Cuts at: 672 1045 1833 2203 2625 3139 4577 672
Size: 373 788 370 422 514 1438 1929
Fragments arranged by size:
1929 1438 788 514 422 373 370
ApaI G_GGCC'C
Cuts at: 1330 1330
Size: 5834
ApaLI G'TGCA_C
Cuts at: 4672 4672
Size: 5834
ApoI r'AATT_y
Cuts at: 2838 5187 5443 2838
Size: 2349 256 3229
Fragments arranged by size:
3229 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5834
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5834
AvaI C'yCGr_G
Cuts at: 698 849 1639 1956 2854 698
Size: 151 790 317 898 3678
Fragments arranged by size:
3678 898 790 317 151
AvrII C'CTAG_G
Cuts at: 2629 2629
Size: 5834
BamHI G'GATC_C
Cuts at: 2859 2859
Size: 5834
BanI G'GyrC_C
Cuts at: 371 874 1157 1400 1549 1705 2002 2605 2850
Size: 503 283 243 149 156 297 603 245
Cuts at: 2850 3264 3512 3547 371
Size: 414 248 35 2658
Fragments arranged by size:
2658 603 503 414 297 283 248 245
243 156 149 35
BanII G_rGCy'C
Cuts at: 142 166 586 739 1067 1330 2543 2848 3878
Size: 24 420 153 328 263 1213 305 1030
Cuts at: 3878 142
Size: 2098
Fragments arranged by size:
2098 1213 1030 420 328 305 263 153
24
BbeI G_GCGC'C
Cuts at: 375 2609 3268 3516 375
Size: 2234 659 248 2693
Fragments arranged by size:
2693 2234 659 248
BbsI GAAGACnn'nnnn_
Cuts at: 312 1959 312
Size: 1647 4187
BbvCI CC'TCA_GC
Cuts at: 1594 1594
Size: 5834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 303 337 2577 2611 303
Size: 34 2240 34 3526
Fragments arranged by size:
3526 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3732 4788 5834 3732
Size: 1056 1046 3732
Fragments arranged by size:
3732 1056 1046
BclI T'GATC_A
Cuts at: 3354 3354
Size: 5834
BglI GCCn_nnn'nGGC
Cuts at: 724 1334 1432 2113 2200 724
Size: 610 98 681 87 4358
Fragments arranged by size:
4358 681 610 98 87
BglII A'GATC_T
Cuts at: 3349 5386 3349
Size: 2037 3797
BlpI GC'TnA_GC
Cuts at: 256 2658 2946 256
Size: 2402 288 3144
Fragments arranged by size:
3144 2402 288
Bme1580I G_kGCm'C
Cuts at: 879 1330 1963 2005 3459 3552 4676 879
Size: 451 633 42 1454 93 1124 2037
Fragments arranged by size:
2037 1454 1124 633 451 93 42
BmgBI CAC'GTC
Cuts at: 2546 2546
Size: 5834
BmrI ACTGGGnnnn_n'
Cuts at: 3046 3127 3462 5606 3046
Size: 81 335 2144 3274
Fragments arranged by size:
3274 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 4201 1942
Size: 2259 3575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 1768 1912 2557 1405
Size: 363 144 645 4682
Fragments arranged by size:
4682 645 363 144
Bpu10I CC'TnA_GC
Cuts at: 1351 1594 1351
Size: 243 5591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 2665 3010 3889 4603 4901 1051
Size: 1614 345 879 714 298 1984
Fragments arranged by size:
1984 1614 879 714 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 1213 1246 1671 5538 1213
Size: 33 425 3867 1509
Fragments arranged by size:
3867 1509 425 33
BsaAI yAC'GTr
Cuts at: 609 2694 3187 609
Size: 2085 493 3256
Fragments arranged by size:
3256 2085 493
BsaBI GATnn'nnATC
Cuts at: 6 3348 5385 6
Size: 3342 2037 455
Fragments arranged by size:
3342 2037 455
BsaHI Gr'CG_yC
Cuts at: 372 1181 2105 2309 2606 3265 3513 4215 5711
Size: 809 924 204 297 659 248 702 1496
Cuts at: 5711 372
Size: 495
Fragments arranged by size:
1496 924 809 702 659 495 297 248
204
BsaWI w'CCGG_w
Cuts at: 360 899 3023 3544 4222 4633 4780 5054 360
Size: 539 2124 521 678 411 147 274 1140
Fragments arranged by size:
2124 1140 678 539 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1642 1672 1693 1723 2071 2101 2530 2560 1642
Size: 30 21 30 348 30 429 30 4916
Fragments arranged by size:
4916 429 348 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 918 1402 1522 2016 918
Size: 484 120 494 4736
Fragments arranged by size:
4736 494 484 120
BseYI C'CCAG_C
Cuts at: 159 860 957 1329 1645 1911 2083 2203 2360
Size: 701 97 372 316 266 172 120 157
Cuts at: 2360 3259 4017 4682 5090 159
Size: 899 758 665 408 903
Fragments arranged by size:
903 899 758 701 665 408 372 316
266 172 157 120 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 898 2015 217
Size: 681 1117 4036
Fragments arranged by size:
4036 1117 681
BsiEI CG_ry'CG
Cuts at: 104 173 440 969 3376 3422 4652 104
Size: 69 267 529 2407 46 1230 1286
Fragments arranged by size:
2407 1286 1230 529 267 69 46
BsiHKAI G_wGCw'C
Cuts at: 142 593 910 2738 2848 3626 3816 4676 142
Size: 451 317 1828 110 778 190 860 1300
Fragments arranged by size:
1828 1300 860 778 451 317 190 110
BsiWI C'GTAC_G
Cuts at: 222 222
Size: 5834
BsmBI CGTCTCn'nnnn_
Cuts at: 1473 2073 1473
Size: 600 5234
Bsp1286I G_dGCh'C
Cuts at: 142 166 586 593 739 879 910 1067 1330
Size: 24 420 7 146 140 31 157 263
Cuts at: 1330 1963 2005 2543 2738 2848 3459 3552 3626
Size: 633 42 538 195 110 611 93 74
Cuts at: 3626 3816 3878 4676 142
Size: 190 62 798 1300
Fragments arranged by size:
1300 798 633 611 538 420 263 195
190 157 146 140 110 93 74 62
42 31 24 7
BspHI T'CATG_A
Cuts at: 4266 4266
Size: 5834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 1412 2884 3098 3400 3781 461
Size: 951 1472 214 302 381 2514
Fragments arranged by size:
2514 1472 951 381 302 214
BsrBI CCG'CTC
Cuts at: 50 270 4126 4180 5664 5829 50
Size: 220 3856 54 1484 165 55
Fragments arranged by size:
3856 1484 220 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3746 3746
Size: 5834
BsrFI r'CCGG_y
Cuts at: 151 968 1333 3832 4013 151
Size: 817 365 2499 181 1972
Fragments arranged by size:
2499 1972 817 365 181
BssHII G'CGCG_C
Cuts at: 307 307
Size: 5834
BssSI C'ACGA_G
Cuts at: 553 1373 4105 4813 553
Size: 820 2732 708 1574
Fragments arranged by size:
2732 1574 820 708
BstAPI GCAn_nnn'nTGC
Cuts at: 921 3211 921
Size: 2290 3544
BstBI TT'CG_AA
Cuts at: 4195 4195
Size: 5834
BstEII G'GTnAC_C
Cuts at: 702 895 1344 702
Size: 193 449 5192
Fragments arranged by size:
5192 449 193
BstXI CCAn_nnnn'nTGG
Cuts at: 747 747
Size: 5834
BstYI r'GATC_y
Cuts at: 1 1816 2859 3326 3349 3684 3930 4236 4248
Size: 1815 1043 467 23 335 246 306 12
Cuts at: 4248 4334 5386 1
Size: 86 1052 449
Fragments arranged by size:
1815 1052 1043 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 880 880
Size: 5834
BtgI C'CryG_G
Cuts at: 576 867 990 1256 1430 1455 1661 1701 1802
Size: 291 123 266 174 25 206 40 101
Cuts at: 1802 2149 576
Size: 347 4261
Fragments arranged by size:
4261 347 291 266 206 174 123 101
40 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2574 3784 3965 4128 2574
Size: 1210 181 163 4280
Fragments arranged by size:
4280 1210 181 163
BtsI GCAGTG_nn'
Cuts at: 285 1849 3077 285
Size: 1564 1228 3042
Fragments arranged by size:
3042 1564 1228
DraI TTT'AAA
Cuts at: 2833 2833
Size: 5834
DraIII CAC_nnn'GTG
Cuts at: 597 1590 597
Size: 993 4841
DrdI GACnn_nn'nnGTC
Cuts at: 361 1132 2803 3540 4884 361
Size: 771 1671 737 1344 1311
Fragments arranged by size:
1671 1344 1311 771 737
EaeI y'GGCC_r
Cuts at: 170 437 966 1727 2235 2576 3419 3593 3984
Size: 267 529 761 508 341 843 174 391
Cuts at: 3984 4011 5356 170
Size: 27 1345 648
Fragments arranged by size:
1345 843 761 648 529 508 391 341
267 174 27
EagI C'GGCC_G
Cuts at: 170 437 966 3419 170
Size: 267 529 2453 2585
Fragments arranged by size:
2585 2453 529 267
EarI CTCTTCn'nnn_
Cuts at: 427 2052 3857 4067 427
Size: 1625 1805 210 2194
Fragments arranged by size:
2194 1805 1625 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 280 1116 4042 4784 4930 5644 280
Size: 836 2926 742 146 714 470
Fragments arranged by size:
2926 836 742 714 470 146
EcoICRI GAG'CTC
Cuts at: 140 2846 140
Size: 2706 3128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 751 1067 1405 1768 1912 2557 3659 4091
Size: 199 316 338 363 144 645 1102 432
Cuts at: 4091 4444 5106 552
Size: 353 662 1280
Fragments arranged by size:
1280 1102 662 645 432 363 353 338
316 199 144
EcoNI CCTnn'n_nnAGG
Cuts at: 1537 1537
Size: 5834
EcoO109I rG'GnC_Cy
Cuts at: 444 1326 1479 1636 1923 2021 2973 444
Size: 882 153 157 287 98 952 3305
Fragments arranged by size:
3305 952 882 287 157 153 98
EcoRI G'AATT_C
Cuts at: 2838 2838
Size: 5834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3853 3853
Size: 5834
FspI TGC'GCA
Cuts at: 334 3615 334
Size: 3281 2553
FspAI rTGC'GCAy
Cuts at: 334 334
Size: 5834
HaeII r_GCGC'y
Cuts at: 244 375 2329 2432 2609 3268 3516 4746 5517
Size: 131 1954 103 177 659 248 1230 771
Cuts at: 5517 244
Size: 561
Fragments arranged by size:
1954 1230 771 659 561 248 177 131
103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 641 673 955 987 1027 1059 1594
Size: 32 494 32 282 32 40 32 535
Cuts at: 1594 1626 1642 1674 1801 1833 2029 2061 2530
Size: 32 16 32 127 32 196 32 469
Cuts at: 2530 2562 3676 3706 3708 3738 115
Size: 32 1114 30 2 30 2211
Fragments arranged by size:
2211 1114 535 494 469 282 196 127
40 32 32 32 32 32 32 32
32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 681 2124 2873 681
Size: 1443 749 3642
Fragments arranged by size:
3642 1443 749
HindIII A'AGCT_T
Cuts at: 1839 1839
Size: 5834
KasI G'GCGC_C
Cuts at: 371 2605 3264 3512 371
Size: 2234 659 248 2693
Fragments arranged by size:
2693 2234 659 248
KpnI G_GTAC'C
Cuts at: 2854 2854
Size: 5834
MluI A'CGCG_T
Cuts at: 5267 5267
Size: 5834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 434 4593 4777 434
Size: 4159 184 1491
Fragments arranged by size:
4159 1491 184
MscI TGG'CCA
Cuts at: 2237 3595 2237
Size: 1358 4476
MslI CAynn'nnrTG
Cuts at: 607 814 872 1030 1210 2669 3823 3950 607
Size: 207 58 158 180 1459 1154 127 2491
Fragments arranged by size:
2491 1459 1154 207 180 158 127 58
MspA1I CmG'CkG
Cuts at: 120 231 417 1061 1999 2100 2375 2945 3259
Size: 111 186 644 938 101 275 570 314
Cuts at: 3259 3619 4403 4648 120
Size: 360 784 245 1306
Fragments arranged by size:
1306 938 784 644 570 360 314 275
245 186 111 101
NaeI GCC'GGC
Cuts at: 1335 4015 1335
Size: 2680 3154
NarI GG'CG_CC
Cuts at: 372 2606 3265 3513 372
Size: 2234 659 248 2693
Fragments arranged by size:
2693 2234 659 248
NcoI C'CATG_G
Cuts at: 1256 1430 1661 1256
Size: 174 231 5429
Fragments arranged by size:
5429 231 174
NgoMIV G'CCGG_C
Cuts at: 1333 4013 1333
Size: 2680 3154
NspI r_CATG'y
Cuts at: 2887 3387 4990 2887
Size: 500 1603 3731
Fragments arranged by size:
3731 1603 500
PasI CC'CwG_GG
Cuts at: 781 975 1827 2017 2583 781
Size: 194 852 190 566 4032
Fragments arranged by size:
4032 852 566 194 190
PciI A'CATG_T
Cuts at: 4986 4986
Size: 5834
PfoI T'CCnGG_A
Cuts at: 1240 1240
Size: 5834
PmeI GTTT'AAAC
Cuts at: 2833 2833
Size: 5834
PmlI CAC'GTG
Cuts at: 2694 2694
Size: 5834
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 4280
Size: 32 5802
PpuMI rG'GwC_Cy
Cuts at: 1923 2021 1923
Size: 98 5736
PspOMI G'GGCC_C
Cuts at: 1326 1326
Size: 5834
PstI C_TGCA'G
Cuts at: 2190 2881 3566 2190
Size: 691 685 4458
Fragments arranged by size:
4458 691 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5834
PvuII CAG'CTG
Cuts at: 231 417 3259 3619 231
Size: 186 2842 360 2446
Fragments arranged by size:
2842 2446 360 186
RsrII CG'GwC_CG
Cuts at: 4029 4029
Size: 5834
SacI G_AGCT'C
Cuts at: 142 2848 142
Size: 2706 3128
SalI G'TCGA_C
Cuts at: 2871 2871
Size: 5834
SapI GCTCTTCn'nnn_
Cuts at: 427 3857 4067 427
Size: 3430 210 2194
Fragments arranged by size:
3430 2194 210
SbfI CC_TGCA'GG
Cuts at: 2881 2881
Size: 5834
ScaI AGT'ACT
Cuts at: 5351 5351
Size: 5834
SexAI A'CCwGG_T
Cuts at: 326 950 1586 326
Size: 624 636 4574
Fragments arranged by size:
4574 636 624
SfcI C'TryA_G
Cuts at: 33 471 1671 2186 2877 3562 4530 4721 33
Size: 438 1200 515 691 685 968 191 1146
Fragments arranged by size:
1200 1146 968 691 685 515 438 191
SfoI GGC'GCC
Cuts at: 373 2607 3266 3514 373
Size: 2234 659 248 2693
Fragments arranged by size:
2693 2234 659 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5834
SmaI CCC'GGG
Cuts at: 700 1641 2856 700
Size: 941 1215 3678
Fragments arranged by size:
3678 1215 941
SmlI C'TyrA_G
Cuts at: 1066 1144 2680 2989 3904 4618 4880 1066
Size: 78 1536 309 915 714 262 2020
Fragments arranged by size:
2020 1536 915 714 309 262 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5834
SphI G_CATG'C
Cuts at: 2887 3387 2887
Size: 500 5334
StuI AGG'CCT
Cuts at: 2767 2767
Size: 5834
StyI C'CwwG_G
Cuts at: 545 1011 1256 1430 1661 1875 1903 2629 2968
Size: 466 245 174 231 214 28 726 339
Cuts at: 2968 3322 545
Size: 354 3057
Fragments arranged by size:
3057 726 466 354 339 245 231 214
174 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 804 1528 2025 2255 3417 3550 4009 4218
Size: 506 724 497 230 1162 133 459 209
Cuts at: 4218 298
Size: 1914
Fragments arranged by size:
1914 1162 724 506 497 459 230 209
133
TatI w'GTAC_w
Cuts at: 761 5349 761
Size: 4588 1246
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1693 1725 5351 5383 1693
Size: 32 3626 32 2144
Fragments arranged by size:
3626 2144 32 32
Tth111I GACn'n_nGTC
Cuts at: 3631 3631
Size: 5834
XbaI T'CTAG_A
Cuts at: 1086 2865 1086
Size: 1779 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 156 390 868 1737 2097 2550 3329 156
Size: 234 478 869 360 453 779 2661
Fragments arranged by size:
2661 869 779 478 453 360 234
XmaI C'CCGG_G
Cuts at: 698 1639 2854 698
Size: 941 1215 3678
Fragments arranged by size:
3678 1215 941
ZraI GAC'GTC
Cuts at: 1182 2310 1182
Size: 1128 4706
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AgeI AscI BaeI BfrBI BmtI BsmI BspEI BsrGI
BstZ17I ClaI CspCI EcoRV FseI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI