Restriction map for
pF1KA1521
(Circular) (Six-base) MAPSORT of: pF1KA1521.seq Check: 4247 from: 1 to: 7547
pF1KA1521 7547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2128 2128
Size: 7547
AatII G_ACGT'C
Cuts at: 2084 2084
Size: 7547
AccI GT'mk_AC
Cuts at: 3768 4426 4585 3768
Size: 658 159 6730
Fragments arranged by size:
6730 658 159
Acc65I G'GTAC_C
Cuts at: 4563 4563
Size: 7547
AclI AA'CG_TT
Cuts at: 3048 7387 3048
Size: 4339 3208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 541 624 686 1196 1856 3008 3629 4177
Size: 224 83 62 510 660 1152 621 548
Cuts at: 4177 5372 5804 6157 6819 317
Size: 1195 432 353 662 1045
Fragments arranged by size:
1195 1152 1045 662 660 621 548 510
432 353 224 83 62
AflII C'TTAA_G
Cuts at: 211 211
Size: 7547
AflIII A'CryG_T
Cuts at: 2156 4899 6699 6980 2156
Size: 2743 1800 281 2723
Fragments arranged by size:
2743 2723 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1591 2150 1591
Size: 559 6988
AlwNI CAG_nnn'CTG
Cuts at: 2143 3415 4852 6290 2143
Size: 1272 1437 1438 3400
Fragments arranged by size:
3400 1438 1437 1272
ApaLI G'TGCA_C
Cuts at: 3176 3353 6385 3176
Size: 177 3032 4338
Fragments arranged by size:
4338 3032 177
ApoI r'AATT_y
Cuts at: 332 400 580 898 1362 2009 3907 4513 4551
Size: 68 180 318 464 647 1898 606 38
Cuts at: 4551 6900 7156 332
Size: 2349 256 723
Fragments arranged by size:
2349 1898 723 647 606 464 318 256
180 68 38
AseI AT'TA_AT
Cuts at: 20 1106 3617 20
Size: 1086 2511 3950
Fragments arranged by size:
3950 2511 1086
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7547
AvaI C'yCGr_G
Cuts at: 2958 4277 4567 2958
Size: 1319 290 5938
Fragments arranged by size:
5938 1319 290
AvrII C'CTAG_G
Cuts at: 607 607
Size: 7547
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2348 2381 2348
Size: 33 7514
BamHI G'GATC_C
Cuts at: 4572 4572
Size: 7547
BanI G'GyrC_C
Cuts at: 2536 4563 4977 5225 5260 2536
Size: 2027 414 248 35 4823
Fragments arranged by size:
4823 2027 414 248 35
BanII G_rGCy'C
Cuts at: 826 2815 3741 4191 4561 5591 826
Size: 1989 926 450 370 1030 2782
Fragments arranged by size:
2782 1989 1030 926 450 370
BbeI G_GCGC'C
Cuts at: 4981 5229 4981
Size: 248 7299
BbsI GAAGACnn'nnnn_
Cuts at: 1280 2462 2594 3575 1280
Size: 1182 132 981 5252
Fragments arranged by size:
5252 1182 981 132
BbvCI CC'TCA_GC
Cuts at: 1496 3194 3719 1496
Size: 1698 525 5324
Fragments arranged by size:
5324 1698 525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3934 3968 3934
Size: 34 7513
BciVI GTATCCnnnnn_n'
Cuts at: 5445 6501 7547 5445
Size: 1056 1046 5445
Fragments arranged by size:
5445 1056 1046
BclI T'GATC_A
Cuts at: 5067 5067
Size: 7547
BglII A'GATC_T
Cuts at: 2464 5062 7099 2464
Size: 2598 2037 2912
Fragments arranged by size:
2912 2598 2037
BlpI GC'TnA_GC
Cuts at: 4659 4659
Size: 7547
Bme1580I G_kGCm'C
Cuts at: 2541 3180 3357 5172 5265 6389 2541
Size: 639 177 1815 93 1124 3699
Fragments arranged by size:
3699 1815 1124 639 177 93
BmrI ACTGGGnnnn_n'
Cuts at: 2347 3236 3511 4759 4840 5175 7319 2347
Size: 889 275 1248 81 335 2144 2575
Fragments arranged by size:
2575 2144 1248 889 335 275 81
BmtI G_CTAG'C
Cuts at: 4460 4460
Size: 7547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 801 803 840 2493 3503 5914 801
Size: 2 37 1653 1010 2411 2434
Fragments arranged by size:
2434 2411 1653 1010 37 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 1406 1490 1502 1670 2091 2341 2732 2882
Size: 930 84 12 168 421 250 391 150
Cuts at: 2882 4379 4490 476
Size: 1497 111 3533
Fragments arranged by size:
3533 1497 930 421 391 250 168 150
111 84 12
Bpu10I CC'TnA_GC
Cuts at: 1496 1996 3185 3194 3719 3956 4330 1496
Size: 500 1189 9 525 237 374 4713
Fragments arranged by size:
4713 1189 525 500 374 237 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 426 1598 2958 3901 4723 5602 6316 6614
Size: 296 1172 1360 943 822 879 714 298
Cuts at: 6614 130
Size: 1063
Fragments arranged by size:
1360 1172 1063 943 879 822 714 298
296
BsaI GGTCTCn'nnnn_
Cuts at: 1267 2059 2832 7251 1267
Size: 792 773 4419 1563
Fragments arranged by size:
4419 1563 792 773
BsaAI yAC'GTr
Cuts at: 4900 4900
Size: 7547
BsaBI GATnn'nnATC
Cuts at: 6 743 2117 3632 5061 7098 6
Size: 737 1374 1515 1429 2037 455
Fragments arranged by size:
2037 1515 1429 1374 737 455
BsaHI Gr'CG_yC
Cuts at: 2081 2868 4978 5226 5928 7424 2081
Size: 787 2110 248 702 1496 2204
Fragments arranged by size:
2204 2110 1496 787 702 248
BsaWI w'CCGG_w
Cuts at: 2834 4076 4736 5257 5935 6346 6493 6767 2834
Size: 1242 660 521 678 411 147 274 3614
Fragments arranged by size:
3614 1242 678 660 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1877 1907 2541 2571 3033 3063 1877
Size: 30 634 30 462 30 6361
Fragments arranged by size:
6361 634 462 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 432 636 2317 2546 2961 2993 4489 432
Size: 204 1681 229 415 32 1496 3490
Fragments arranged by size:
3490 1681 1496 415 229 204 32
BseYI C'CCAG_C
Cuts at: 1676 2386 2854 3160 4972 5730 6395 6803 1676
Size: 710 468 306 1812 758 665 408 2420
Fragments arranged by size:
2420 1812 758 710 665 468 408 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1286 2196 2493 2918 4220 4309 4453 1286
Size: 910 297 425 1302 89 144 4380
Fragments arranged by size:
4380 1302 910 425 297 144 89
BsiEI CG_ry'CG
Cuts at: 104 5089 5135 6365 104
Size: 4985 46 1230 1286
Fragments arranged by size:
4985 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2815 3180 3357 4191 4312 4561 5339 5529 6389
Size: 365 177 834 121 249 778 190 860
Cuts at: 6389 2815
Size: 3973
Fragments arranged by size:
3973 860 834 778 365 249 190 177
121
BsmI GAATG_Cn'
Cuts at: 2764 2778 3225 2764
Size: 14 447 7086
Fragments arranged by size:
7086 447 14
Bsp1286I G_dGCh'C
Cuts at: 826 2541 2815 3180 3357 3741 4191 4312 4561
Size: 1715 274 365 177 384 450 121 249
Cuts at: 4561 5172 5265 5339 5529 5591 6389 826
Size: 611 93 74 190 62 798 1984
Fragments arranged by size:
1984 1715 798 611 450 384 365 274
249 190 177 121 93 74 62
BspEI T'CCGG_A
Cuts at: 2834 2834
Size: 7547
BspHI T'CATG_A
Cuts at: 3285 4140 5979 3285
Size: 855 1839 4853
Fragments arranged by size:
4853 1839 855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1125 2128 4597 4811 5113 5494 1125
Size: 1003 2469 214 302 381 3178
Fragments arranged by size:
3178 2469 1003 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4363 5839 5893 7377 7542 50
Size: 4313 1476 54 1484 165 55
Fragments arranged by size:
4313 1484 1476 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1025 1169 3260 3998 5459 1025
Size: 144 2091 738 1461 3113
Fragments arranged by size:
3113 2091 1461 738 144
BsrFI r'CCGG_y
Cuts at: 2864 5545 5726 2864
Size: 2681 181 4685
Fragments arranged by size:
4685 2681 181
BsrGI T'GTAC_A
Cuts at: 968 968
Size: 7547
BssSI C'ACGA_G
Cuts at: 5818 6526 5818
Size: 708 6839
BstAPI GCAn_nnn'nTGC
Cuts at: 247 2143 4924 247
Size: 1896 2781 2870
Fragments arranged by size:
2870 2781 1896
BstBI TT'CG_AA
Cuts at: 2802 5908 2802
Size: 3106 4441
BstXI CCAn_nnnn'nTGG
Cuts at: 336 336
Size: 7547
BstYI r'GATC_y
Cuts at: 1 759 1185 2464 2673 4572 5039 5062 5397
Size: 758 426 1279 209 1899 467 23 335
Cuts at: 5397 5643 5949 5961 6047 7099 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1899 1279 1052 758 467 449 426 335
306 246 209 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1662 1662
Size: 7547
BtgI C'CryG_G
Cuts at: 107 1773 4225 107
Size: 1666 2452 3429
Fragments arranged by size:
3429 2452 1666
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2642 3020 5497 5678 5841 2642
Size: 378 2477 181 163 4348
Fragments arranged by size:
4348 2477 378 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1818 3379 4297 4790 1274
Size: 544 1561 918 493 4031
Fragments arranged by size:
4031 1561 918 544 493
ClaI AT'CG_AT
Cuts at: 1536 3846 4529 1536
Size: 2310 683 4554
Fragments arranged by size:
4554 2310 683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 863 898 4400 4435 863
Size: 35 3502 35 3975
Fragments arranged by size:
3975 3502 35 35
DraI TTT'AAA
Cuts at: 654 4546 654
Size: 3892 3655
DraIII CAC_nnn'GTG
Cuts at: 487 487
Size: 7547
DrdI GACnn_nn'nnGTC
Cuts at: 5253 6597 5253
Size: 1344 6203
EaeI y'GGCC_r
Cuts at: 1445 2582 3839 4052 4228 4337 5132 5306 5697
Size: 1137 1257 213 176 109 795 174 391
Cuts at: 5697 5724 7069 1445
Size: 27 1345 1923
Fragments arranged by size:
1923 1345 1257 1137 795 391 213 176
174 109 27
EagI C'GGCC_G
Cuts at: 5132 5132
Size: 7547
EarI CTCTTCn'nnn_
Cuts at: 1172 1366 1637 2876 4196 5570 5780 1172
Size: 194 271 1239 1320 1374 210 2939
Fragments arranged by size:
2939 1374 1320 1239 271 210 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5755 6497 6643 7357 5755
Size: 742 146 714 5945
Fragments arranged by size:
5945 742 714 146
EcoICRI GAG'CTC
Cuts at: 2813 4189 4559 2813
Size: 1376 370 5801
Fragments arranged by size:
5801 1376 370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 476 541 624 686 1196 1406 1490 1502
Size: 159 65 83 62 510 210 84 12
Cuts at: 1502 1670 1856 2091 2341 2732 2882 3008 3629
Size: 168 186 235 250 391 150 126 621
Cuts at: 3629 4177 4379 4490 5372 5804 6157 6819 317
Size: 548 202 111 882 432 353 662 1045
Fragments arranged by size:
1045 882 662 621 548 510 432 391
353 250 235 210 202 186 168 159
150 126 111 84 83 65 62 12
EcoNI CCTnn'n_nnAGG
Cuts at: 148 556 2296 148
Size: 408 1740 5399
Fragments arranged by size:
5399 1740 408
EcoO109I rG'GnC_Cy
Cuts at: 1672 1710 1840 1912 2283 3127 4686 1672
Size: 38 130 72 371 844 1559 4533
Fragments arranged by size:
4533 1559 844 371 130 72 38
EcoRI G'AATT_C
Cuts at: 400 3907 4513 4551 400
Size: 3507 606 38 3396
Fragments arranged by size:
3507 3396 606 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2264 2798 4051 4110 5566 2264
Size: 534 1253 59 1456 4245
Fragments arranged by size:
4245 1456 1253 534 59
FspI TGC'GCA
Cuts at: 5328 5328
Size: 7547
HaeII r_GCGC'y
Cuts at: 4981 5229 6459 7230 4981
Size: 248 1230 771 5298
Fragments arranged by size:
5298 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 408 440 945 977 1879 1911 2449 2481 2541
Size: 32 505 32 902 32 538 32 60
Cuts at: 2541 2573 2584 2616 2809 2841 3001 3033 3124
Size: 32 11 32 193 32 160 32 91
Cuts at: 3124 3156 4164 4196 5389 5419 5421 5451 408
Size: 32 1008 32 1193 30 2 30 2504
Fragments arranged by size:
2504 1193 1008 902 538 505 193 160
91 60 32 32 32 32 32 32
32 32 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 3769 4586 3769
Size: 817 6730
HindIII A'AGCT_T
Cuts at: 299 2246 299
Size: 1947 5600
KasI G'GCGC_C
Cuts at: 4977 5225 4977
Size: 248 7299
KpnI G_GTAC'C
Cuts at: 4567 4567
Size: 7547
MfeI C'AATT_G
Cuts at: 370 522 370
Size: 152 7395
MluI A'CGCG_T
Cuts at: 6980 6980
Size: 7547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 6306 6490 6306
Size: 184 7363
MscI TGG'CCA
Cuts at: 2584 3841 4054 4230 4339 5308 2584
Size: 1257 213 176 109 969 4823
Fragments arranged by size:
4823 1257 969 213 176 109
MslI CAynn'nnrTG
Cuts at: 1573 5536 5663 1573
Size: 3963 127 3457
Fragments arranged by size:
3963 3457 127
MspA1I CmG'CkG
Cuts at: 220 1947 2547 2752 2858 2902 3940 4050 4658
Size: 1727 600 205 106 44 1038 110 608
Cuts at: 4658 4972 5332 6116 6361 220
Size: 314 360 784 245 1406
Fragments arranged by size:
1727 1406 1038 784 608 600 360 314
245 205 110 106 44
NaeI GCC'GGC
Cuts at: 5728 5728
Size: 7547
NarI GG'CG_CC
Cuts at: 4978 5226 4978
Size: 248 7299
NcoI C'CATG_G
Cuts at: 107 1773 4225 107
Size: 1666 2452 3429
Fragments arranged by size:
3429 2452 1666
NdeI CA'TA_TG
Cuts at: 1592 4009 1592
Size: 2417 5130
NgoMIV G'CCGG_C
Cuts at: 5726 5726
Size: 7547
NheI G'CTAG_C
Cuts at: 4456 4456
Size: 7547
NruI TCG'CGA
Cuts at: 4127 4127
Size: 7547
NspI r_CATG'y
Cuts at: 328 1722 2160 4600 5100 6703 328
Size: 1394 438 2440 500 1603 1172
Fragments arranged by size:
2440 1603 1394 1172 500 438
PciI A'CATG_T
Cuts at: 2156 6699 2156
Size: 4543 3004
PflMI CCAn_nnn'nTGG
Cuts at: 2728 2728
Size: 7547
PfoI T'CCnGG_A
Cuts at: 1625 2684 2871 1625
Size: 1059 187 6301
Fragments arranged by size:
6301 1059 187
PmeI GTTT'AAAC
Cuts at: 4546 4546
Size: 7547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5993 6025 5993
Size: 32 7515
PpuMI rG'GwC_Cy
Cuts at: 1672 1710 1840 1912 1672
Size: 38 130 72 7307
Fragments arranged by size:
7307 130 72 38
PsiI TTA'TAA
Cuts at: 4268 4268
Size: 7547
PstI C_TGCA'G
Cuts at: 1062 1813 2148 2907 3386 3977 4594 5279 1062
Size: 751 335 759 479 591 617 685 3330
Fragments arranged by size:
3330 759 751 685 617 591 479 335
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7547
PvuII CAG'CTG
Cuts at: 220 1947 2547 2752 2858 2902 3940 4050 4972
Size: 1727 600 205 106 44 1038 110 922
Cuts at: 4972 5332 220
Size: 360 2435
Fragments arranged by size:
2435 1727 1038 922 600 360 205 110
106 44
RsrII CG'GwC_CG
Cuts at: 5742 5742
Size: 7547
SacI G_AGCT'C
Cuts at: 2815 4191 4561 2815
Size: 1376 370 5801
Fragments arranged by size:
5801 1376 370
SalI G'TCGA_C
Cuts at: 3767 4584 3767
Size: 817 6730
SapI GCTCTTCn'nnn_
Cuts at: 2876 4196 5570 5780 2876
Size: 1320 1374 210 4643
Fragments arranged by size:
4643 1374 1320 210
SbfI CC_TGCA'GG
Cuts at: 4594 4594
Size: 7547
ScaI AGT'ACT
Cuts at: 7064 7064
Size: 7547
SexAI A'CCwGG_T
Cuts at: 4130 4130
Size: 7547
SfcI C'TryA_G
Cuts at: 33 1058 1338 1544 1809 1942 2144 2903 3382
Size: 1025 280 206 265 133 202 759 479
Cuts at: 3382 3726 3778 3973 4590 5275 6243 6434 33
Size: 344 52 195 617 685 968 191 1146
Fragments arranged by size:
1146 1025 968 759 685 617 479 344
280 265 206 202 195 191 133 52
SfoI GGC'GCC
Cuts at: 4979 5227 4979
Size: 248 7299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7547
SmaI CCC'GGG
Cuts at: 4279 4569 4279
Size: 290 7257
SmlI C'TyrA_G
Cuts at: 145 211 405 1613 2973 3880 4702 5617 6331
Size: 66 194 1208 1360 907 822 915 714
Cuts at: 6331 6593 145
Size: 262 1099
Fragments arranged by size:
1360 1208 1099 915 907 822 714 262
194 66
SpeI A'CTAG_T
Cuts at: 67 2506 2796 67
Size: 2439 290 4818
Fragments arranged by size:
4818 2439 290
SphI G_CATG'C
Cuts at: 4600 5100 4600
Size: 500 7047
SspI AAT'ATT
Cuts at: 2109 2109
Size: 7547
StuI AGG'CCT
Cuts at: 2399 2399
Size: 7547
StyI C'CwwG_G
Cuts at: 107 607 1206 1773 3034 4225 4681 5035 107
Size: 500 599 567 1261 1191 456 354 2619
Fragments arranged by size:
2619 1261 1191 599 567 500 456 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3328 4550 5130 5263 5722 5931 3328
Size: 1222 580 133 459 209 4944
Fragments arranged by size:
4944 1222 580 459 209 133
TatI w'GTAC_w
Cuts at: 207 968 1024 2074 3271 7062 207
Size: 761 56 1050 1197 3791 692
Fragments arranged by size:
3791 1197 1050 761 692 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1877 1909 3141 3173 4245 4277 7064 7096 1877
Size: 32 1232 32 1072 32 2787 32 2328
Fragments arranged by size:
2787 2328 1232 1072 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4351 5344 4351
Size: 993 6554
XbaI T'CTAG_A
Cuts at: 4578 4578
Size: 7547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1578 5042 1578
Size: 3464 4083
XmaI C'CCGG_G
Cuts at: 4277 4567 4277
Size: 290 7257
XmnI GAAnn'nnTTC
Cuts at: 1089 3911 3968 4034 1089
Size: 2822 57 66 4602
Fragments arranged by size:
4602 2822 66 57
ZraI GAC'GTC
Cuts at: 2082 2082
Size: 7547
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NruI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AhdI AleI AloI ApaI AscI BfrBI
BglI BmgBI BsiWI BsmBI BssHII BstEII BstZ17I EcoRV
FseI FspAI HpaI NotI NsiI PacI PasI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI