Restriction map for
pF1KA1520
(Circular) (Six-base) MAPSORT of: pF1KA1520.seq Check: 6153 from: 1 to: 5411
pF1KA1520 5411 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1287 1287
Size: 5411
AccI GT'mk_AC
Cuts at: 1502 2449 1502
Size: 947 4464
Acc65I G'GTAC_C
Cuts at: 2427 2427
Size: 5411
AclI AA'CG_TT
Cuts at: 5251 5251
Size: 5411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 1671 2326 3236 3668 4415 4683 171
Size: 1500 655 910 432 747 268 899
Fragments arranged by size:
1500 910 899 747 655 432 268
AfeI AGC'GCT
Cuts at: 2121 2121
Size: 5411
AflIII A'CryG_T
Cuts at: 1334 2763 3867 4844 1334
Size: 1429 1104 977 1901
Fragments arranged by size:
1901 1429 1104 977
AgeI A'CCGG_T
Cuts at: 3799 3799
Size: 5411
AleI CACnn'nnGTG
Cuts at: 370 1054 370
Size: 684 4727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 5411
AlwNI CAG_nnn'CTG
Cuts at: 546 636 1671 2286 2289 2334 2716 4283 546
Size: 90 1035 615 3 45 382 1567 1674
Fragments arranged by size:
1674 1567 1035 615 382 90 45 3
ApaI G_GGCC'C
Cuts at: 335 462 1727 335
Size: 127 1265 4019
Fragments arranged by size:
4019 1265 127
ApaLI G'TGCA_C
Cuts at: 1576 4181 1576
Size: 2605 2806
ApoI r'AATT_y
Cuts at: 2415 4764 5020 2415
Size: 2349 256 2806
Fragments arranged by size:
2806 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5411
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
AvaI C'yCGr_G
Cuts at: 604 1728 2069 2431 604
Size: 1124 341 362 3584
Fragments arranged by size:
3584 1124 362 341
BamHI G'GATC_C
Cuts at: 2436 2436
Size: 5411
BanI G'GyrC_C
Cuts at: 535 2026 2272 2427 2841 3089 3124 535
Size: 1491 246 155 414 248 35 2822
Fragments arranged by size:
2822 1491 414 248 246 155 35
BanII G_rGCy'C
Cuts at: 335 462 1361 1498 1727 1747 1874 2076 2425
Size: 127 899 137 229 20 127 202 349
Cuts at: 2425 3455 335
Size: 1030 2291
Fragments arranged by size:
2291 1030 899 349 229 202 137 127
127 20
BbeI G_GCGC'C
Cuts at: 2030 2845 3093 2030
Size: 815 248 4348
Fragments arranged by size:
4348 815 248
BbsI GAAGACnn'nnnn_
Cuts at: 177 1017 1056 1240 177
Size: 840 39 184 4348
Fragments arranged by size:
4348 840 184 39
BbvCI CC'TCA_GC
Cuts at: 572 572
Size: 5411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 774 808 1425 1459 1557 1591 90
Size: 34 650 34 617 34 98 34 3910
Fragments arranged by size:
3910 650 617 98 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3309 4070 5411 3309
Size: 761 1341 3309
Fragments arranged by size:
3309 1341 761
BclI T'GATC_A
Cuts at: 2931 2931
Size: 5411
BglI GCCn_nnn'nGGC
Cuts at: 339 568 339
Size: 229 5182
BglII A'GATC_T
Cuts at: 2926 4963 2926
Size: 2037 3374
BlpI GC'TnA_GC
Cuts at: 2209 2523 2209
Size: 314 5097
Bme1580I G_kGCm'C
Cuts at: 335 462 1580 1727 2275 3036 3129 4185 335
Size: 127 1118 147 548 761 93 1056 1561
Fragments arranged by size:
1561 1118 1056 761 548 147 127 93
BmgBI CAC'GTC
Cuts at: 660 660
Size: 5411
BmrI ACTGGGnnnn_n'
Cuts at: 2086 2623 2704 3039 5183 2086
Size: 537 81 335 2144 2314
Fragments arranged by size:
2314 2144 537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3778 3778
Size: 5411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 568 1627 1783 1801 1975 232
Size: 336 1059 156 18 174 3668
Fragments arranged by size:
3668 1059 336 174 156 18
Bpu10I CC'TnA_GC
Cuts at: 572 572
Size: 5411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2587 3466 3958 4256 2587
Size: 879 492 298 3742
Fragments arranged by size:
3742 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 706 1011 1210 5115 706
Size: 305 199 3905 1002
Fragments arranged by size:
3905 1002 305 199
BsaAI yAC'GTr
Cuts at: 2764 2764
Size: 5411
BsaBI GATnn'nnATC
Cuts at: 6 2925 4962 6
Size: 2919 2037 455
Fragments arranged by size:
2919 2037 455
BsaHI Gr'CG_yC
Cuts at: 613 2027 2842 3090 3792 5288 613
Size: 1414 815 248 702 1496 736
Fragments arranged by size:
1496 1414 815 736 702 248
BsaWI w'CCGG_w
Cuts at: 1241 2600 3121 3799 4073 4220 4631 1241
Size: 1359 521 678 274 147 411 2021
Fragments arranged by size:
2021 1359 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 949 979 991 1021 949
Size: 30 12 30 5339
Fragments arranged by size:
5339 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1228 1273 1276 1279 1228
Size: 45 3 3 5360
Fragments arranged by size:
5360 45 3 3
BseYI C'CCAG_C
Cuts at: 291 327 2030 2334 2836 3594 4171 4667 291
Size: 36 1703 304 502 758 577 496 1035
Fragments arranged by size:
1703 1035 758 577 502 496 304 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2284 2341 2284
Size: 57 5354
BsiEI CG_ry'CG
Cuts at: 104 594 1560 2953 2999 4207 104
Size: 490 966 1393 46 1208 1308
Fragments arranged by size:
1393 1308 1208 966 490 46
BsiHKAI G_wGCw'C
Cuts at: 1580 1747 2215 2225 2425 3203 3393 4185 1580
Size: 167 468 10 200 778 190 792 2806
Fragments arranged by size:
2806 792 778 468 200 190 167 10
BsmI GAATG_Cn'
Cuts at: 1188 1188
Size: 5411
Bsp1286I G_dGCh'C
Cuts at: 335 462 1361 1498 1580 1727 1747 1874 2076
Size: 127 899 137 82 147 20 127 202
Cuts at: 2076 2215 2225 2275 2425 3036 3129 3203 3393
Size: 139 10 50 150 611 93 74 190
Cuts at: 3393 3455 4185 335
Size: 62 730 1561
Fragments arranged by size:
1561 899 730 611 202 190 150 147
139 137 127 127 93 82 74 62
50 20 10
BspEI T'CCGG_A
Cuts at: 1241 4631 1241
Size: 3390 2021
BspHI T'CATG_A
Cuts at: 146 1124 4587 146
Size: 978 3463 970
Fragments arranged by size:
3463 978 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 1287 1289 2180 2461 2675 2977 3358 826
Size: 461 2 891 281 214 302 381 2879
Fragments arranged by size:
2879 891 461 381 302 281 214 2
BsrBI CCG'CTC
Cuts at: 50 590 908 1889 3703 3757 5241 5406 50
Size: 540 318 981 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 981 540 318 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3323 3323
Size: 5411
BsrFI r'CCGG_y
Cuts at: 593 1559 2204 3409 3590 3799 593
Size: 966 645 1205 181 209 2205
Fragments arranged by size:
2205 1205 966 645 209 181
BsrGI T'GTAC_A
Cuts at: 1301 1301
Size: 5411
BssSI C'ACGA_G
Cuts at: 351 370 670 3682 4040 351
Size: 19 300 3012 358 1722
Fragments arranged by size:
3012 1722 358 300 19
BstAPI GCAn_nnn'nTGC
Cuts at: 636 2788 636
Size: 2152 3259
BstBI TT'CG_AA
Cuts at: 891 3772 891
Size: 2881 2530
BstEII G'GTnAC_C
Cuts at: 1098 1098
Size: 5411
BstXI CCAn_nnnn'nTGG
Cuts at: 309 2124 309
Size: 1815 3596
BstYI r'GATC_y
Cuts at: 1 252 2436 2903 2926 3261 3507 4519 4605
Size: 251 2184 467 23 335 246 1012 86
Cuts at: 4605 4617 4963 1
Size: 12 346 449
Fragments arranged by size:
2184 1012 467 449 346 335 251 246
86 23 12
BtgI C'CryG_G
Cuts at: 170 398 989 1490 1571 1971 2063 2163 2216
Size: 228 591 501 81 400 92 100 53
Cuts at: 2216 170
Size: 3365
Fragments arranged by size:
3365 591 501 400 228 100 92 81
53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1428 2181 3361 3542 3705 1428
Size: 753 1180 181 163 3134
Fragments arranged by size:
3134 1180 753 181 163
BtsI GCAGTG_nn'
Cuts at: 1363 1740 2654 1363
Size: 377 914 4120
Fragments arranged by size:
4120 914 377
DraI TTT'AAA
Cuts at: 2410 2410
Size: 5411
DraIII CAC_nnn'GTG
Cuts at: 1848 1848
Size: 5411
DrdI GACnn_nn'nnGTC
Cuts at: 3117 3975 3117
Size: 858 4553
EaeI y'GGCC_r
Cuts at: 173 311 529 560 591 595 674 812 1190
Size: 138 218 31 31 4 79 138 378
Cuts at: 1190 2043 2060 2367 2996 3170 3561 3588 4933
Size: 853 17 307 629 174 391 27 1345
Cuts at: 4933 173
Size: 651
Fragments arranged by size:
1345 853 651 629 391 378 307 218
174 138 138 79 31 31 27 17
4
EagI C'GGCC_G
Cuts at: 591 2996 591
Size: 2405 3006
EarI CTCTTCn'nnn_
Cuts at: 385 421 907 1735 3434 3644 385
Size: 36 486 828 1699 210 2152
Fragments arranged by size:
2152 1699 828 486 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 211 1225 3619 3929 4075 5221 211
Size: 1014 2394 310 146 1146 401
Fragments arranged by size:
2394 1146 1014 401 310 146
EcoICRI GAG'CTC
Cuts at: 1745 2423 1745
Size: 678 4733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 232 568 1627 1671 1783 1801 1975 2326
Size: 61 336 1059 44 112 18 174 351
Cuts at: 2326 3236 3668 4415 4683 171
Size: 910 432 747 268 899
Fragments arranged by size:
1059 910 899 747 432 351 336 268
174 112 61 44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 211 1516 211
Size: 1305 4106
EcoO109I rG'GnC_Cy
Cuts at: 331 332 444 459 543 957 1668 1723 1724
Size: 1 112 15 84 414 711 55 1
Cuts at: 1724 2550 331
Size: 826 3192
Fragments arranged by size:
3192 826 711 414 112 84 55 15
1 1
EcoRI G'AATT_C
Cuts at: 2415 2415
Size: 5411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3430 3430
Size: 5411
FseI GG_CCGG'CC
Cuts at: 597 597
Size: 5411
FspI TGC'GCA
Cuts at: 430 3192 430
Size: 2762 2649
HaeII r_GCGC'y
Cuts at: 1823 2030 2123 2845 3093 4115 5094 1823
Size: 207 93 722 248 1022 979 2140
Fragments arranged by size:
2140 1022 979 722 248 207 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 949 981 991 1023 1891 1923 2127
Size: 32 319 32 10 32 868 32 204
Cuts at: 2127 2159 3253 3283 3285 3315 598
Size: 32 1094 30 2 30 2694
Fragments arranged by size:
2694 1094 868 319 204 32 32 32
32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 1755 2450 1755
Size: 695 4716
HindIII A'AGCT_T
Cuts at: 930 930
Size: 5411
KasI G'GCGC_C
Cuts at: 2026 2841 3089 2026
Size: 815 248 4348
Fragments arranged by size:
4348 815 248
KpnI G_GTAC'C
Cuts at: 2431 2431
Size: 5411
MluI A'CGCG_T
Cuts at: 4844 4844
Size: 5411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1160 4082 4266 1160
Size: 2922 184 2305
Fragments arranged by size:
2922 2305 184
MscI TGG'CCA
Cuts at: 313 814 1192 2045 2062 2369 3172 313
Size: 501 378 853 17 307 803 2552
Fragments arranged by size:
2552 853 803 501 378 307 17
MslI CAynn'nnrTG
Cuts at: 151 163 307 370 988 1054 1105 1123 1126
Size: 12 144 63 618 66 51 18 3
Cuts at: 1126 1129 2239 3400 3527 151
Size: 3 1110 1161 127 2035
Fragments arranged by size:
2035 1161 1110 618 144 127 66 63
51 18 12 3 3
MspA1I CmG'CkG
Cuts at: 281 1327 1355 1433 2034 2055 2286 2325 2365
Size: 1046 28 78 601 21 231 39 40
Cuts at: 2365 2522 2836 3196 4209 4454 281
Size: 157 314 360 1013 245 1238
Fragments arranged by size:
1238 1046 1013 601 360 314 245 231
157 78 40 39 28 21
NaeI GCC'GGC
Cuts at: 595 3592 595
Size: 2997 2414
NarI GG'CG_CC
Cuts at: 2027 2842 3090 2027
Size: 815 248 4348
Fragments arranged by size:
4348 815 248
NcoI C'CATG_G
Cuts at: 398 989 1571 2063 2163 398
Size: 591 582 492 100 3646
Fragments arranged by size:
3646 591 582 492 100
NgoMIV G'CCGG_C
Cuts at: 593 3590 593
Size: 2997 2414
NspI r_CATG'y
Cuts at: 1338 2464 2964 3871 1338
Size: 1126 500 907 2878
Fragments arranged by size:
2878 1126 907 500
PasI CC'CwG_GG
Cuts at: 583 2295 583
Size: 1712 3699
PciI A'CATG_T
Cuts at: 1334 3867 1334
Size: 2533 2878
PflMI CCAn_nnn'nTGG
Cuts at: 546 546
Size: 5411
PfoI T'CCnGG_A
Cuts at: 209 440 1760 1991 2102 209
Size: 231 1320 231 111 3518
Fragments arranged by size:
3518 1320 231 231 111
PmeI GTTT'AAAC
Cuts at: 2410 2410
Size: 5411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1528 1560 4550 4582 1528
Size: 32 2990 32 2357
Fragments arranged by size:
2990 2357 32 32
PpuMI rG'GwC_Cy
Cuts at: 444 957 1668 444
Size: 513 711 4187
Fragments arranged by size:
4187 711 513
PspOMI G'GGCC_C
Cuts at: 331 458 1723 331
Size: 127 1265 4019
Fragments arranged by size:
4019 1265 127
PstI C_TGCA'G
Cuts at: 632 1295 1676 2177 2458 3143 632
Size: 663 381 501 281 685 2900
Fragments arranged by size:
2900 685 663 501 381 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5411
PvuII CAG'CTG
Cuts at: 281 1327 2034 2286 2365 2836 3196 281
Size: 1046 707 252 79 471 360 2496
Fragments arranged by size:
2496 1046 707 471 360 252 79
RsrII CG'GwC_CG
Cuts at: 3606 3606
Size: 5411
SacI G_AGCT'C
Cuts at: 1747 2425 1747
Size: 678 4733
SalI G'TCGA_C
Cuts at: 2448 2448
Size: 5411
SanDI GG'GwC_CC
Cuts at: 444 444
Size: 5411
SapI GCTCTTCn'nnn_
Cuts at: 421 1735 3434 3644 421
Size: 1314 1699 210 2188
Fragments arranged by size:
2188 1699 1314 210
SbfI CC_TGCA'GG
Cuts at: 2458 2458
Size: 5411
ScaI AGT'ACT
Cuts at: 1153 1837 4928 1153
Size: 684 3091 1636
Fragments arranged by size:
3091 1636 684
SexAI A'CCwGG_T
Cuts at: 1001 1001
Size: 5411
SfcI C'TryA_G
Cuts at: 33 628 1291 1503 1672 2001 2173 2381 2454
Size: 595 663 212 169 329 172 208 73
Cuts at: 2454 3139 4132 4323 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 663 595 329 212 208
191 172 169 73
SfoI GGC'GCC
Cuts at: 2028 2843 3091 2028
Size: 815 248 4348
Fragments arranged by size:
4348 815 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
SmaI CCC'GGG
Cuts at: 2071 2433 2071
Size: 362 5049
SmlI C'TyrA_G
Cuts at: 2566 3481 3973 4235 2566
Size: 915 492 262 3742
Fragments arranged by size:
3742 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5411
SphI G_CATG'C
Cuts at: 2464 2964 2464
Size: 500 4911
SrfI GCCC'GGGC
Cuts at: 2071 2071
Size: 5411
StyI C'CwwG_G
Cuts at: 398 989 1094 1571 2063 2163 2545 2899 398
Size: 591 105 477 492 100 382 354 2910
Fragments arranged by size:
2910 591 492 477 382 354 105 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 1678 2290 2994 3127 3586 3795 553
Size: 1125 612 704 133 459 209 2169
Fragments arranged by size:
2169 1125 704 612 459 209 133
TatI w'GTAC_w
Cuts at: 1151 1301 1337 1835 4926 1151
Size: 150 36 498 3091 1636
Fragments arranged by size:
3091 1636 498 150 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4928 4960 4928
Size: 32 5379
Tth111I GACn'n_nGTC
Cuts at: 1003 3208 1003
Size: 2205 3206
XbaI T'CTAG_A
Cuts at: 2442 2442
Size: 5411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 657 1125 2038 2121 2906 306
Size: 351 468 913 83 785 2811
Fragments arranged by size:
2811 913 785 468 351 83
XmaI C'CCGG_G
Cuts at: 2069 2431 2069
Size: 362 5049
XmnI GAAnn'nnTTC
Cuts at: 1543 1543
Size: 5411
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
EcoRV FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SspI StuI SwaI XhoI ZraI