Restriction map for
pF1KA1485
(Circular) (Six-base) MAPSORT of: pF1KA1485.seq Check: 8694 from: 1 to: 6842
pF1KA1485 6842 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1067 1117 2111 1067
Size: 50 994 5798
Fragments arranged by size:
5798 994 50
AatII G_ACGT'C
Cuts at: 1205 1205
Size: 6842
AccI GT'mk_AC
Cuts at: 3737 3773 3880 3737
Size: 36 107 6699
Fragments arranged by size:
6699 107 36
Acc65I G'GTAC_C
Cuts at: 2363 3858 2363
Size: 1495 5347
AclI AA'CG_TT
Cuts at: 6682 6682
Size: 6842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 2521 2779 3154 4667 5099 5846 6114 794
Size: 1727 258 375 1513 432 747 268 1522
Fragments arranged by size:
1727 1522 1513 747 432 375 268 258
AflIII A'CryG_T
Cuts at: 2927 4194 5298 6275 2927
Size: 1267 1104 977 3494
Fragments arranged by size:
3494 1267 1104 977
AgeI A'CCGG_T
Cuts at: 2231 5230 2231
Size: 2999 3843
AleI CACnn'nnGTG
Cuts at: 1331 2921 3490 1331
Size: 1590 569 4683
Fragments arranged by size:
4683 1590 569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 1571 1837 538
Size: 1033 266 5543
Fragments arranged by size:
5543 1033 266
AlwNI CAG_nnn'CTG
Cuts at: 1446 2173 3051 3447 4147 5714 1446
Size: 727 878 396 700 1567 2574
Fragments arranged by size:
2574 1567 878 727 700 396
ApaI G_GGCC'C
Cuts at: 776 776
Size: 6842
ApaLI G'TGCA_C
Cuts at: 1054 1135 1150 1546 1786 3009 5612 1054
Size: 81 15 396 240 1223 2603 2284
Fragments arranged by size:
2603 2284 1223 396 240 81 15
ApoI r'AATT_y
Cuts at: 528 795 2941 3846 6195 6451 528
Size: 267 2146 905 2349 256 919
Fragments arranged by size:
2349 2146 919 905 267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6842
AvaI C'yCGr_G
Cuts at: 2402 2701 3862 2402
Size: 299 1161 5382
Fragments arranged by size:
5382 1161 299
AvrII C'CTAG_G
Cuts at: 2067 2067
Size: 6842
BamHI G'GATC_C
Cuts at: 980 2309 3867 980
Size: 1329 1558 3955
Fragments arranged by size:
3955 1558 1329
BanI G'GyrC_C
Cuts at: 822 2053 2363 3184 3259 3523 3570 3858 4272
Size: 1231 310 821 75 264 47 288 414
Cuts at: 4272 4520 4555 822
Size: 248 35 3109
Fragments arranged by size:
3109 1231 821 414 310 288 264 248
75 47 35
BanII G_rGCy'C
Cuts at: 776 1280 1415 2065 2814 3368 3617 3856 4886
Size: 504 135 650 749 554 249 239 1030
Cuts at: 4886 776
Size: 2732
Fragments arranged by size:
2732 1030 749 650 554 504 249 239
135
BbeI G_GCGC'C
Cuts at: 2057 3527 4276 4524 2057
Size: 1470 749 248 4375
Fragments arranged by size:
4375 1470 749 248
BbsI GAAGACnn'nnnn_
Cuts at: 936 1566 3410 936
Size: 630 1844 4368
Fragments arranged by size:
4368 1844 630
BbvCI CC'TCA_GC
Cuts at: 2144 2144
Size: 6842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1488 1522 1488
Size: 34 6808
BciVI GTATCCnnnnn_n'
Cuts at: 4740 5501 6842 4740
Size: 761 1341 4740
Fragments arranged by size:
4740 1341 761
BclI T'GATC_A
Cuts at: 1997 4362 1997
Size: 2365 4477
BfrBI ATG'CAT
Cuts at: 2385 2385
Size: 6842
BglI GCCn_nnn'nGGC
Cuts at: 1039 1564 1039
Size: 525 6317
BglII A'GATC_T
Cuts at: 656 4357 6394 656
Size: 3701 2037 1104
Fragments arranged by size:
3701 2037 1104
BlpI GC'TnA_GC
Cuts at: 3954 3954
Size: 6842
Bme1580I G_kGCm'C
Cuts at: 776 1012 1058 1139 1154 1550 1790 3013 3099
Size: 236 46 81 15 396 240 1223 86
Cuts at: 3099 3187 4467 4560 5616 776
Size: 88 1280 93 1056 2002
Fragments arranged by size:
2002 1280 1223 1056 396 240 236 93
88 86 81 46 15
BmgBI CAC'GTC
Cuts at: 3711 3711
Size: 6842
BmrI ACTGGGnnnn_n'
Cuts at: 1008 3369 4054 4135 4470 6614 1008
Size: 2361 685 81 335 2144 1236
Fragments arranged by size:
2361 2144 1236 685 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 426 3384 3636 5209 426
Size: 2958 252 1573 2059
Fragments arranged by size:
2958 2059 1573 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1666 1753 2150 2353 2519 3424 1094
Size: 572 87 397 203 166 905 4512
Fragments arranged by size:
4512 905 572 397 203 166 87
Bpu10I CC'TnA_GC
Cuts at: 1114 2144 2225 1114
Size: 1030 81 5731
Fragments arranged by size:
5731 1030 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 1114 1684 1859 2863 4018 4897 5389 5687
Size: 844 570 175 1004 1155 879 492 298
Cuts at: 5687 270
Size: 1425
Fragments arranged by size:
1425 1155 1004 879 844 570 492 298
175
BsaI GGTCTCn'nnnn_
Cuts at: 796 1857 2321 2679 3320 3523 6546 796
Size: 1061 464 358 641 203 3023 1092
Fragments arranged by size:
3023 1092 1061 641 464 358 203
BsaAI yAC'GTr
Cuts at: 2472 3777 4195 2472
Size: 1305 418 5119
Fragments arranged by size:
5119 1305 418
BsaBI GATnn'nnATC
Cuts at: 6 4356 6393 6
Size: 4350 2037 455
Fragments arranged by size:
4350 2037 455
BsaHI Gr'CG_yC
Cuts at: 1202 2054 3524 3539 4273 4521 5223 6719 1202
Size: 852 1470 15 734 248 702 1496 1325
Fragments arranged by size:
1496 1470 1325 852 734 702 248 15
BsaWI w'CCGG_w
Cuts at: 295 420 2231 2312 4031 4552 5230 5504 5651
Size: 125 1811 81 1719 521 678 274 147
Cuts at: 5651 6062 295
Size: 411 1075
Fragments arranged by size:
1811 1719 1075 678 521 411 274 147
125 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1780 1810 3339 3369 1780
Size: 30 1529 30 5253
Fragments arranged by size:
5253 1529 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 385 553 1684 1750 1845 1848 1908 2149
Size: 207 168 1131 66 95 3 60 241
Cuts at: 2149 2721 3181 3376 3622 3625 3817 178
Size: 572 460 195 246 3 192 3203
Fragments arranged by size:
3203 1131 572 460 246 241 207 195
192 168 95 66 60 3 3
BseYI C'CCAG_C
Cuts at: 769 1779 2029 2116 2139 3519 3616 4267 5025
Size: 1010 250 87 23 1380 97 651 758
Cuts at: 5025 5602 6098 769
Size: 577 496 1513
Fragments arranged by size:
1513 1380 1010 758 651 577 496 250
97 87 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 707 1038 3799 707
Size: 331 2761 3750
Fragments arranged by size:
3750 2761 331
BsiEI CG_ry'CG
Cuts at: 104 3474 4384 4430 5638 104
Size: 3370 910 46 1208 1308
Fragments arranged by size:
3370 1308 1208 910 46
BsiHKAI G_wGCw'C
Cuts at: 1058 1139 1154 1280 1415 1550 1790 2231 2686
Size: 81 15 126 135 135 240 441 455
Cuts at: 2686 3013 3212 3856 4634 4824 5616 1058
Size: 327 199 644 778 190 792 2284
Fragments arranged by size:
2284 792 778 644 455 441 327 240
199 190 135 135 126 81 15
BsmI GAATG_Cn'
Cuts at: 1698 1869 1698
Size: 171 6671
BsmBI CGTCTCn'nnnn_
Cuts at: 106 1223 3640 3718 106
Size: 1117 2417 78 3230
Fragments arranged by size:
3230 2417 1117 78
Bsp1286I G_dGCh'C
Cuts at: 776 1012 1058 1139 1154 1280 1415 1550 1790
Size: 236 46 81 15 126 135 135 240
Cuts at: 1790 2065 2231 2686 2814 3013 3099 3187 3212
Size: 275 166 455 128 199 86 88 25
Cuts at: 3212 3368 3617 3856 4467 4560 4634 4824 4886
Size: 156 249 239 611 93 74 190 62
Cuts at: 4886 5616 776
Size: 730 2002
Fragments arranged by size:
2002 730 611 455 275 249 240 239
236 199 190 166 156 135 135 128
126 93 88 86 81 74 62 46
25 15
BspEI T'CCGG_A
Cuts at: 420 2312 6062 420
Size: 1892 3750 1200
Fragments arranged by size:
3750 1892 1200
BspHI T'CATG_A
Cuts at: 314 2252 2774 2814 3635 6018 314
Size: 1938 522 40 821 2383 1138
Fragments arranged by size:
2383 1938 1138 821 522 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 1067 1117 1874 2111 3588 3892 4106 4408
Size: 523 50 757 237 1477 304 214 302
Cuts at: 4408 4789 544
Size: 381 2597
Fragments arranged by size:
2597 1477 757 523 381 304 302 237
214 50
BsrBI CCG'CTC
Cuts at: 50 2307 5134 5188 6672 6837 50
Size: 2257 2827 54 1484 165 55
Fragments arranged by size:
2827 2257 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3099 4754 3099
Size: 1655 5187
BsrFI r'CCGG_y
Cuts at: 2231 3822 4840 5021 5230 2231
Size: 1591 1018 181 209 3843
Fragments arranged by size:
3843 1591 1018 209 181
BssSI C'ACGA_G
Cuts at: 5113 5471 5113
Size: 358 6484
BstAPI GCAn_nnn'nTGC
Cuts at: 963 4219 963
Size: 3256 3586
BstBI TT'CG_AA
Cuts at: 5203 5203
Size: 6842
BstEII G'GTnAC_C
Cuts at: 2104 3600 2104
Size: 1496 5346
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3223 2029
Size: 1194 5648
BstYI r'GATC_y
Cuts at: 1 656 980 2112 2309 3867 4334 4357 4692
Size: 655 324 1132 197 1558 467 23 335
Cuts at: 4692 4938 5950 6036 6048 6394 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1558 1132 1012 655 467 449 346 335
324 246 197 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 253 338 976 2669 3359 253
Size: 85 638 1693 690 3736
Fragments arranged by size:
3736 1693 690 638 85
BtgI C'CryG_G
Cuts at: 107 625 1383 1422 1847 2049 3485 3566 3665
Size: 518 758 39 425 202 1436 81 99
Cuts at: 3665 107
Size: 3284
Fragments arranged by size:
3284 1436 758 518 425 202 99 81
39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4792 4973 5136 4792
Size: 181 163 6498
Fragments arranged by size:
6498 181 163
BtsI GCAGTG_nn'
Cuts at: 1170 2984 3542 4085 1170
Size: 1814 558 543 3927
Fragments arranged by size:
3927 1814 558 543
ClaI AT'CG_AT
Cuts at: 2634 2634
Size: 6842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3531 3566 3531
Size: 35 6807
DraI TTT'AAA
Cuts at: 3841 3841
Size: 6842
DraIII CAC_nnn'GTG
Cuts at: 1831 3017 3693 1831
Size: 1186 676 4980
Fragments arranged by size:
4980 1186 676
DrdI GACnn_nn'nnGTC
Cuts at: 1255 4548 5406 1255
Size: 3293 858 2691
Fragments arranged by size:
3293 2691 858
EaeI y'GGCC_r
Cuts at: 134 1419 3431 4427 4601 4992 5019 6364 134
Size: 1285 2012 996 174 391 27 1345 612
Fragments arranged by size:
2012 1345 1285 996 612 391 174 27
EagI C'GGCC_G
Cuts at: 4427 4427
Size: 6842
EarI CTCTTCn'nnn_
Cuts at: 169 313 319 362 1661 1700 4865 5075 169
Size: 144 6 43 1299 39 3165 210 1936
Fragments arranged by size:
3165 1936 1299 210 144 43 39 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5050 5360 5506 6652 5050
Size: 310 146 1146 5240
Fragments arranged by size:
5240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1278 1413 3854 1278
Size: 135 2441 4266
Fragments arranged by size:
4266 2441 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 1094 1666 1753 2150 2353 2519 2521 2779
Size: 300 572 87 397 203 166 2 258
Cuts at: 2779 3154 3424 4667 5099 5846 6114 794
Size: 375 270 1243 432 747 268 1522
Fragments arranged by size:
1522 1243 747 572 432 397 375 300
270 268 258 203 166 87 2
EcoNI CCTnn'n_nnAGG
Cuts at: 782 830 1117 782
Size: 48 287 6507
Fragments arranged by size:
6507 287 48
EcoO109I rG'GnC_Cy
Cuts at: 773 898 1758 2259 3444 3981 773
Size: 125 860 501 1185 537 3634
Fragments arranged by size:
3634 1185 860 537 501 125
EcoRI G'AATT_C
Cuts at: 3846 3846
Size: 6842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1488 4861 1488
Size: 3373 3469
FspI TGC'GCA
Cuts at: 1063 1528 4623 1063
Size: 465 3095 3282
Fragments arranged by size:
3282 3095 465
HaeII r_GCGC'y
Cuts at: 1447 2057 3527 4276 4524 5546 6525 1447
Size: 610 1470 749 248 1022 979 1764
Fragments arranged by size:
1764 1470 1022 979 749 610 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1084 1116 1903 1935 2218 2250 2649 2681 4684
Size: 32 787 32 283 32 399 32 2003
Cuts at: 4684 4714 4716 4746 1084
Size: 30 2 30 3180
Fragments arranged by size:
3180 2003 787 399 283 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3881 3881
Size: 6842
KasI G'GCGC_C
Cuts at: 2053 3523 4272 4520 2053
Size: 1470 749 248 4375
Fragments arranged by size:
4375 1470 749 248
KpnI G_GTAC'C
Cuts at: 2367 3862 2367
Size: 1495 5347
MluI A'CGCG_T
Cuts at: 6275 6275
Size: 6842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 421 454 509 3647 3728 5513 5697 161
Size: 260 33 55 3138 81 1785 184 1306
Fragments arranged by size:
3138 1785 1306 260 184 81 55 33
MscI TGG'CCA
Cuts at: 1421 4603 1421
Size: 3182 3660
MslI CAynn'nnrTG
Cuts at: 142 520 709 989 1005 1331 2089 2819 2921
Size: 378 189 280 16 326 758 730 102
Cuts at: 2921 3161 3490 3634 4831 4958 142
Size: 240 329 144 1197 127 2026
Fragments arranged by size:
2026 1197 758 730 378 329 326 280
240 189 144 127 102 16
MspA1I CmG'CkG
Cuts at: 498 1311 1659 1731 1783 2101 2143 2718 3031
Size: 813 348 72 52 318 42 575 313
Cuts at: 3031 3048 3092 3202 3427 3953 4267 4627 5640
Size: 17 44 110 225 526 314 360 1013
Cuts at: 5640 5885 498
Size: 245 1455
Fragments arranged by size:
1455 1013 813 575 526 360 348 318
314 313 245 225 110 72 52 44
42 17
NaeI GCC'GGC
Cuts at: 3824 5023 3824
Size: 1199 5643
NarI GG'CG_CC
Cuts at: 2054 3524 4273 4521 2054
Size: 1470 749 248 4375
Fragments arranged by size:
4375 1470 749 248
NcoI C'CATG_G
Cuts at: 107 1422 2049 3566 107
Size: 1315 627 1517 3383
Fragments arranged by size:
3383 1517 1315 627
NdeI CA'TA_TG
Cuts at: 1373 2888 1373
Size: 1515 5327
NgoMIV G'CCGG_C
Cuts at: 3822 5021 3822
Size: 1199 5643
NsiI A_TGCA'T
Cuts at: 2387 2387
Size: 6842
NspI r_CATG'y
Cuts at: 1459 1962 2389 2931 3073 3895 4395 5302 1459
Size: 503 427 542 142 822 500 907 2999
Fragments arranged by size:
2999 907 822 542 503 500 427 142
PasI CC'CwG_GG
Cuts at: 1762 1762
Size: 6842
PciI A'CATG_T
Cuts at: 2927 5298 2927
Size: 2371 4471
PflMI CCAn_nnn'nTGG
Cuts at: 1377 1446 2173 3693 1377
Size: 69 727 1520 4526
Fragments arranged by size:
4526 1520 727 69
PfoI T'CCnGG_A
Cuts at: 1607 3440 3641 1607
Size: 1833 201 4808
Fragments arranged by size:
4808 1833 201
PmeI GTTT'AAAC
Cuts at: 3841 3841
Size: 6842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1327 1359 5981 6013 1327
Size: 32 4622 32 2156
Fragments arranged by size:
4622 2156 32 32
PpuMI rG'GwC_Cy
Cuts at: 898 2259 3444 898
Size: 1361 1185 4296
Fragments arranged by size:
4296 1361 1185
PshAI GACnn'nnGTC
Cuts at: 627 627
Size: 6842
PspOMI G'GGCC_C
Cuts at: 772 772
Size: 6842
PstI C_TGCA'G
Cuts at: 968 2991 3506 3889 4574 968
Size: 2023 515 383 685 3236
Fragments arranged by size:
3236 2023 685 515 383
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6842
PvuII CAG'CTG
Cuts at: 498 1311 1659 1731 1783 2101 2143 2718 3048
Size: 813 348 72 52 318 42 575 330
Cuts at: 3048 4267 4627 498
Size: 1219 360 2713
Fragments arranged by size:
2713 1219 813 575 360 348 330 318
72 52 42
RsrII CG'GwC_CG
Cuts at: 5037 5037
Size: 6842
SacI G_AGCT'C
Cuts at: 1280 1415 3856 1280
Size: 135 2441 4266
Fragments arranged by size:
4266 2441 135
SalI G'TCGA_C
Cuts at: 3879 3879
Size: 6842
SapI GCTCTTCn'nnn_
Cuts at: 4865 5075 4865
Size: 210 6632
SbfI CC_TGCA'GG
Cuts at: 3889 3889
Size: 6842
ScaI AGT'ACT
Cuts at: 1195 6359 1195
Size: 5164 1678
SexAI A'CCwGG_T
Cuts at: 818 1614 3689 818
Size: 796 2075 3971
Fragments arranged by size:
3971 2075 796
SfcI C'TryA_G
Cuts at: 33 964 1654 2551 2987 3502 3885 4570 5563
Size: 931 690 897 436 515 383 685 993
Cuts at: 5563 5754 33
Size: 191 1121
Fragments arranged by size:
1121 993 931 897 690 685 515 436
383 191
SfoI GGC'GCC
Cuts at: 2055 3525 4274 4522 2055
Size: 1470 749 248 4375
Fragments arranged by size:
4375 1470 749 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6842
SmaI CCC'GGG
Cuts at: 2404 3864 2404
Size: 1460 5382
SmlI C'TyrA_G
Cuts at: 285 1129 1699 1874 2878 3997 4912 5404 5666
Size: 844 570 175 1004 1119 915 492 262
Cuts at: 5666 285
Size: 1461
Fragments arranged by size:
1461 1119 1004 915 844 570 492 262
175
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6842
SphI G_CATG'C
Cuts at: 3895 4395 3895
Size: 500 6342
StuI AGG'CCT
Cuts at: 258 336 1267 1567 1711 2149 3757 258
Size: 78 931 300 144 438 1608 3343
Fragments arranged by size:
3343 1608 931 438 300 144 78
StyI C'CwwG_G
Cuts at: 107 1268 1422 1568 2015 2049 2067 3566 3976
Size: 1161 154 146 447 34 18 1499 410
Cuts at: 3976 4330 107
Size: 354 2619
Fragments arranged by size:
2619 1499 1161 447 410 354 154 146
34 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 1546 2410 3388 3488 4425 4558 5017 5226
Size: 1023 864 978 100 937 133 459 209
Cuts at: 5226 523
Size: 2139
Fragments arranged by size:
2139 1023 978 937 864 459 209 133
100
TatI w'GTAC_w
Cuts at: 265 1193 6357 265
Size: 928 5164 750
Fragments arranged by size:
5164 928 750
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1780 1812 6359 6391 1780
Size: 32 4547 32 2231
Fragments arranged by size:
4547 2231 32 32
Tth111I GACn'n_nGTC
Cuts at: 4639 4639
Size: 6842
XbaI T'CTAG_A
Cuts at: 3873 3873
Size: 6842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 3795 4337 708
Size: 3087 542 3213
Fragments arranged by size:
3213 3087 542
XmaI C'CCGG_G
Cuts at: 2402 3862 2402
Size: 1460 5382
XmnI GAAnn'nnTTC
Cuts at: 734 2830 3407 734
Size: 2096 577 4169
Fragments arranged by size:
4169 2096 577
ZraI GAC'GTC
Cuts at: 1203 1203
Size: 6842
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI BaeI BmtI BsiWI
BsrGI BssHII BstZ17I EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PmlI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI