Restriction map for
pF1KA1477
(Circular) (Six-base) MAPSORT of: pF1KA1477.seq Check: 6374 from: 1 to: 5387
pF1KA1477 5387 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1941 1941
Size: 5387
AccI GT'mk_AC
Cuts at: 408 2425 408
Size: 2017 3370
Acc65I G'GTAC_C
Cuts at: 2403 2403
Size: 5387
AclI AA'CG_TT
Cuts at: 5227 5227
Size: 5387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 925 1228 1282 1781 3212 3644 4391 4659
Size: 155 303 54 499 1431 432 747 268
Cuts at: 4659 770
Size: 1498
Fragments arranged by size:
1498 1431 747 499 432 303 268 155
54
AflII C'TTAA_G
Cuts at: 589 589
Size: 5387
AflIII A'CryG_T
Cuts at: 342 2739 3843 4820 342
Size: 2397 1104 977 909
Fragments arranged by size:
2397 1104 977 909
AgeI A'CCGG_T
Cuts at: 3775 3775
Size: 5387
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 176 208 176
Size: 32 5355
AlwNI CAG_nnn'CTG
Cuts at: 2692 4259 2692
Size: 1567 3820
ApaLI G'TGCA_C
Cuts at: 4157 4157
Size: 5387
ApoI r'AATT_y
Cuts at: 315 526 658 822 1162 2017 2391 4740 4996
Size: 211 132 164 340 855 374 2349 256
Cuts at: 4996 315
Size: 706
Fragments arranged by size:
2349 855 706 374 340 256 211 164
132
AseI AT'TA_AT
Cuts at: 20 20
Size: 5387
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
AvaI C'yCGr_G
Cuts at: 715 1856 2407 715
Size: 1141 551 3695
Fragments arranged by size:
3695 1141 551
BamHI G'GATC_C
Cuts at: 2412 2412
Size: 5387
BanI G'GyrC_C
Cuts at: 2403 2817 3065 3100 2403
Size: 414 248 35 4690
Fragments arranged by size:
4690 414 248 35
BanII G_rGCy'C
Cuts at: 1867 2401 3431 1867
Size: 534 1030 3823
Fragments arranged by size:
3823 1030 534
BbeI G_GCGC'C
Cuts at: 2821 3069 2821
Size: 248 5139
BbsI GAAGACnn'nnnn_
Cuts at: 2122 2122
Size: 5387
BbvCI CC'TCA_GC
Cuts at: 1677 1677
Size: 5387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1441 1475 1441
Size: 34 5353
BciVI GTATCCnnnnn_n'
Cuts at: 3285 4046 5387 3285
Size: 761 1341 3285
Fragments arranged by size:
3285 1341 761
BclI T'GATC_A
Cuts at: 1311 2907 1311
Size: 1596 3791
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5387
BglII A'GATC_T
Cuts at: 2902 4939 2902
Size: 2037 3350
BlpI GC'TnA_GC
Cuts at: 2499 2499
Size: 5387
Bme1580I G_kGCm'C
Cuts at: 3012 3105 4161 3012
Size: 93 1056 4238
Fragments arranged by size:
4238 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1211 2599 2680 3015 5159 1211
Size: 1388 81 335 2144 1439
Fragments arranged by size:
2144 1439 1388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3754 3754
Size: 5387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1820 1547
Size: 273 5114
Bpu10I CC'TnA_GC
Cuts at: 1677 1677
Size: 5387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1851 2563 3442 3934 4232 1851
Size: 712 879 492 298 3006
Fragments arranged by size:
3006 879 712 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5091 5091
Size: 5387
BsaAI yAC'GTr
Cuts at: 1584 2740 1584
Size: 1156 4231
BsaBI GATnn'nnATC
Cuts at: 6 2901 4938 6
Size: 2895 2037 455
Fragments arranged by size:
2895 2037 455
BsaHI Gr'CG_yC
Cuts at: 121 773 2818 3066 3768 5264 121
Size: 652 2045 248 702 1496 244
Fragments arranged by size:
2045 1496 702 652 248 244
BsaWI w'CCGG_w
Cuts at: 1023 1841 2576 3097 3775 4049 4196 4607 1023
Size: 818 735 521 678 274 147 411 1803
Fragments arranged by size:
1803 818 735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1818 1848 1818
Size: 30 5357
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1408 1408
Size: 5387
BseYI C'CCAG_C
Cuts at: 2812 3570 4147 4643 2812
Size: 758 577 496 3556
Fragments arranged by size:
3556 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 2275
Size: 5387
BsiEI CG_ry'CG
Cuts at: 104 2929 2975 4183 104
Size: 2825 46 1208 1308
Fragments arranged by size:
2825 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1867 2401 3179 3369 4161 1867
Size: 534 778 190 792 3093
Fragments arranged by size:
3093 792 778 534 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2225 2225
Size: 5387
Bsp1286I G_dGCh'C
Cuts at: 1867 2401 3012 3105 3179 3369 3431 4161 1867
Size: 534 611 93 74 190 62 730 3093
Fragments arranged by size:
3093 730 611 534 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1023 4607 1023
Size: 3584 1803
BspHI T'CATG_A
Cuts at: 984 4563 984
Size: 3579 1808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1941 2437 2651 2953 3334 1941
Size: 496 214 302 381 3994
Fragments arranged by size:
3994 496 381 302 214
BsrBI CCG'CTC
Cuts at: 50 144 3679 3733 5217 5382 50
Size: 94 3535 54 1484 165 55
Fragments arranged by size:
3535 1484 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 124 1588 2348 3299 124
Size: 1464 760 951 2212
Fragments arranged by size:
2212 1464 951 760
BsrFI r'CCGG_y
Cuts at: 1769 3385 3566 3775 1769
Size: 1616 181 209 3381
Fragments arranged by size:
3381 1616 209 181
BsrGI T'GTAC_A
Cuts at: 549 549
Size: 5387
BssSI C'ACGA_G
Cuts at: 1076 2251 3658 4016 1076
Size: 1175 1407 358 2447
Fragments arranged by size:
2447 1407 1175 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2764 2764
Size: 5387
BstBI TT'CG_AA
Cuts at: 3748 3748
Size: 5387
BstEII G'GTnAC_C
Cuts at: 1059 1059
Size: 5387
BstYI r'GATC_y
Cuts at: 1 2412 2879 2902 3237 3483 4495 4581 4593
Size: 2411 467 23 335 246 1012 86 12
Cuts at: 4593 4939 1
Size: 346 449
Fragments arranged by size:
2411 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1361 1361
Size: 5387
BtgI C'CryG_G
Cuts at: 492 1064 1884 2226 492
Size: 572 820 342 3653
Fragments arranged by size:
3653 820 572 342
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3337 3518 3681 3337
Size: 181 163 5043
Fragments arranged by size:
5043 181 163
BtsI GCAGTG_nn'
Cuts at: 1735 2263 2630 1735
Size: 528 367 4492
Fragments arranged by size:
4492 528 367
ClaI AT'CG_AT
Cuts at: 966 1578 966
Size: 612 4775
DraI TTT'AAA
Cuts at: 827 2386 827
Size: 1559 3828
DrdI GACnn_nn'nnGTC
Cuts at: 1057 3093 3951 1057
Size: 2036 858 2493
Fragments arranged by size:
2493 2036 858
EaeI y'GGCC_r
Cuts at: 816 2972 3146 3537 3564 4909 816
Size: 2156 174 391 27 1345 1294
Fragments arranged by size:
2156 1345 1294 391 174 27
EagI C'GGCC_G
Cuts at: 2972 2972
Size: 5387
EarI CTCTTCn'nnn_
Cuts at: 983 1247 1659 2060 3410 3620 983
Size: 264 412 401 1350 210 2750
Fragments arranged by size:
2750 1350 412 401 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3595 3905 4051 5197 3595
Size: 310 146 1146 3785
Fragments arranged by size:
3785 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1865 2399 1865
Size: 534 4853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 925 1228 1282 1547 1781 1820 3212 3644
Size: 155 303 54 265 234 39 1392 432
Cuts at: 3644 4391 4659 770
Size: 747 268 1498
Fragments arranged by size:
1498 1392 747 432 303 268 265 234
155 54 39
EcoNI CCTnn'n_nnAGG
Cuts at: 461 1472 461
Size: 1011 4376
EcoO109I rG'GnC_Cy
Cuts at: 1208 2526 1208
Size: 1318 4069
EcoRI G'AATT_C
Cuts at: 2391 2391
Size: 5387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3406 3406
Size: 5387
FspI TGC'GCA
Cuts at: 3168 3168
Size: 5387
HaeII r_GCGC'y
Cuts at: 2821 3069 4091 5070 2821
Size: 248 1022 979 3138
Fragments arranged by size:
3138 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 209 241 435 467 1560 1592 3229 3259 3261
Size: 32 194 32 1093 32 1637 30 2
Cuts at: 3261 3291 209
Size: 30 2305
Fragments arranged by size:
2305 1637 1093 194 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1988 2426 1988
Size: 438 4949
HindIII A'AGCT_T
Cuts at: 1763 1763
Size: 5387
KasI G'GCGC_C
Cuts at: 2817 3065 2817
Size: 248 5139
KpnI G_GTAC'C
Cuts at: 2407 2407
Size: 5387
MluI A'CGCG_T
Cuts at: 4820 4820
Size: 5387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1419 4058 4242 1419
Size: 2639 184 2564
Fragments arranged by size:
2639 2564 184
MscI TGG'CCA
Cuts at: 818 3148 818
Size: 2330 3057
MslI CAynn'nnrTG
Cuts at: 3376 3503 3376
Size: 127 5260
MspA1I CmG'CkG
Cuts at: 1299 2498 2812 3172 4185 4430 1299
Size: 1199 314 360 1013 245 2256
Fragments arranged by size:
2256 1199 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3568 3568
Size: 5387
NarI GG'CG_CC
Cuts at: 2818 3066 2818
Size: 248 5139
NcoI C'CATG_G
Cuts at: 492 1064 1884 2226 492
Size: 572 820 342 3653
Fragments arranged by size:
3653 820 572 342
NdeI CA'TA_TG
Cuts at: 1554 1554
Size: 5387
NgoMIV G'CCGG_C
Cuts at: 3566 3566
Size: 5387
NspI r_CATG'y
Cuts at: 1972 2440 2940 3847 1972
Size: 468 500 907 3512
Fragments arranged by size:
3512 907 500 468
PciI A'CATG_T
Cuts at: 3843 3843
Size: 5387
PfoI T'CCnGG_A
Cuts at: 1898 1898
Size: 5387
PmeI GTTT'AAAC
Cuts at: 2386 2386
Size: 5387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 4526
Size: 32 5355
PpuMI rG'GwC_Cy
Cuts at: 1208 1208
Size: 5387
PsiI TTA'TAA
Cuts at: 1922 1922
Size: 5387
PstI C_TGCA'G
Cuts at: 1650 2434 3119 1650
Size: 784 685 3918
Fragments arranged by size:
3918 784 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5387
PvuII CAG'CTG
Cuts at: 2812 3172 2812
Size: 360 5027
RsrII CG'GwC_CG
Cuts at: 3582 3582
Size: 5387
SacI G_AGCT'C
Cuts at: 1867 2401 1867
Size: 534 4853
SalI G'TCGA_C
Cuts at: 2424 2424
Size: 5387
SapI GCTCTTCn'nnn_
Cuts at: 1659 3410 3620 1659
Size: 1751 210 3426
Fragments arranged by size:
3426 1751 210
SbfI CC_TGCA'GG
Cuts at: 2434 2434
Size: 5387
ScaI AGT'ACT
Cuts at: 1827 4904 1827
Size: 3077 2310
SfcI C'TryA_G
Cuts at: 33 1646 2430 3115 4108 4299 33
Size: 1613 784 685 993 191 1121
Fragments arranged by size:
1613 1121 993 784 685 191
SfoI GGC'GCC
Cuts at: 2819 3067 2819
Size: 248 5139
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5387
SmaI CCC'GGG
Cuts at: 2409 2409
Size: 5387
SmlI C'TyrA_G
Cuts at: 589 1866 2542 3457 3949 4211 589
Size: 1277 676 915 492 262 1765
Fragments arranged by size:
1765 1277 915 676 492 262
SnaBI TAC'GTA
Cuts at: 1584 1584
Size: 5387
SpeI A'CTAG_T
Cuts at: 67 2122 67
Size: 2055 3332
SphI G_CATG'C
Cuts at: 2440 2940 2440
Size: 500 4887
SspI AAT'ATT
Cuts at: 627 627
Size: 5387
StyI C'CwwG_G
Cuts at: 492 726 1064 1884 2226 2521 2875 492
Size: 234 338 820 342 295 354 3004
Fragments arranged by size:
3004 820 354 342 338 295 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2970 3103 3562 3771 2970
Size: 133 459 209 4586
Fragments arranged by size:
4586 459 209 133
TatI w'GTAC_w
Cuts at: 549 569 1825 2032 4902 549
Size: 20 1256 207 2870 1034
Fragments arranged by size:
2870 1256 1034 207 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1684 1716 4904 4936 1684
Size: 32 3188 32 2135
Fragments arranged by size:
3188 2135 32 32
Tth111I GACn'n_nGTC
Cuts at: 3184 3184
Size: 5387
XbaI T'CTAG_A
Cuts at: 2418 2418
Size: 5387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 330 2127 2882 330
Size: 1797 755 2835
Fragments arranged by size:
2835 1797 755
XmaI C'CCGG_G
Cuts at: 2407 2407
Size: 5387
XmnI GAAnn'nnTTC
Cuts at: 602 899 602
Size: 297 5090
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BssHII BstXI
BstZ17I CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI