Restriction map for
pF1KA1475
(Circular) (Six-base) MAPSORT of: pF1KA1475.seq Check: 343 from: 1 to: 6032
pF1KA1475 6032 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 449 1152 2019 2292 2680 449
Size: 703 867 273 388 3801
Fragments arranged by size:
3801 867 703 388 273
AccI GT'mk_AC
Cuts at: 2255 3070 2255
Size: 815 5217
Acc65I G'GTAC_C
Cuts at: 3048 3048
Size: 6032
AclI AA'CG_TT
Cuts at: 5872 5872
Size: 6032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2398 3857 4289 5036 5304 1225
Size: 1173 1459 432 747 268 1953
Fragments arranged by size:
1953 1459 1173 747 432 268
AfeI AGC'GCT
Cuts at: 2998 2998
Size: 6032
AflII C'TTAA_G
Cuts at: 2533 2533
Size: 6032
AflIII A'CryG_T
Cuts at: 3384 4488 5465 3384
Size: 1104 977 3951
Fragments arranged by size:
3951 1104 977
AgeI A'CCGG_T
Cuts at: 4420 4420
Size: 6032
AleI CACnn'nnGTG
Cuts at: 720 720
Size: 6032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 738 738
Size: 6032
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 127
Size: 32 6000
AlwNI CAG_nnn'CTG
Cuts at: 718 2562 2625 3337 4904 718
Size: 1844 63 712 1567 1846
Fragments arranged by size:
1846 1844 1567 712 63
ApaI G_GGCC'C
Cuts at: 748 2888 748
Size: 2140 3892
ApaLI G'TGCA_C
Cuts at: 1146 4802 1146
Size: 3656 2376
ApoI r'AATT_y
Cuts at: 1192 3036 5385 5641 1192
Size: 1844 2349 256 1583
Fragments arranged by size:
2349 1844 1583 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
AvaI C'yCGr_G
Cuts at: 195 950 1292 1388 2846 3052 195
Size: 755 342 96 1458 206 3175
Fragments arranged by size:
3175 1458 755 342 206 96
BamHI G'GATC_C
Cuts at: 3057 3057
Size: 6032
BanI G'GyrC_C
Cuts at: 1007 3048 3462 3710 3745 1007
Size: 2041 414 248 35 3294
Fragments arranged by size:
3294 2041 414 248 35
BanII G_rGCy'C
Cuts at: 368 629 748 848 1601 1623 2654 2842 2888
Size: 261 119 100 753 22 1031 188 46
Cuts at: 2888 3046 4076 368
Size: 158 1030 2324
Fragments arranged by size:
2324 1031 1030 753 261 188 158 119
100 46 22
BbeI G_GCGC'C
Cuts at: 1011 3466 3714 1011
Size: 2455 248 3329
Fragments arranged by size:
3329 2455 248
BbsI GAAGACnn'nnnn_
Cuts at: 1257 1257
Size: 6032
BbvCI CC'TCA_GC
Cuts at: 824 1687 824
Size: 863 5169
BciVI GTATCCnnnnn_n'
Cuts at: 321 3930 4691 6032 321
Size: 3609 761 1341 321
Fragments arranged by size:
3609 1341 761 321
BclI T'GATC_A
Cuts at: 1790 3552 1790
Size: 1762 4270
BglI GCCn_nnn'nGGC
Cuts at: 1393 1726 2639 2857 1393
Size: 333 913 218 4568
Fragments arranged by size:
4568 913 333 218
BglII A'GATC_T
Cuts at: 3547 5584 3547
Size: 2037 3995
BlpI GC'TnA_GC
Cuts at: 1024 3144 1024
Size: 2120 3912
Bme1580I G_kGCm'C
Cuts at: 233 565 748 1150 2598 2888 3657 3750 4806
Size: 332 183 402 1448 290 769 93 1056
Cuts at: 4806 233
Size: 1459
Fragments arranged by size:
1459 1448 1056 769 402 332 290 183
93
BmrI ACTGGGnnnn_n'
Cuts at: 568 1095 3244 3325 3660 5804 568
Size: 527 2149 81 335 2144 796
Fragments arranged by size:
2149 2144 796 527 335 81
BmtI G_CTAG'C
Cuts at: 2444 2444
Size: 6032
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4399 4399
Size: 6032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 279 1207 1447 1510 2176 2812 238
Size: 41 928 240 63 666 636 3458
Fragments arranged by size:
3458 928 666 636 240 63 41
Bpu10I CC'TnA_GC
Cuts at: 454 824 1606 1687 454
Size: 370 782 81 4799
Fragments arranged by size:
4799 782 370 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1465 2182 2440 2887 3208 4087 4579 4877
Size: 714 717 258 447 321 879 492 298
Cuts at: 4877 751
Size: 1906
Fragments arranged by size:
1906 879 717 714 492 447 321 298
258
BsaI GGTCTCn'nnnn_
Cuts at: 2606 5736 2606
Size: 3130 2902
BsaAI yAC'GTr
Cuts at: 375 477 690 1278 2394 3385 375
Size: 102 213 588 1116 991 3022
Fragments arranged by size:
3022 1116 991 588 213 102
BsaBI GATnn'nnATC
Cuts at: 6 3546 5583 6
Size: 3540 2037 455
Fragments arranged by size:
3540 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 1008 3463 3711 4413 5909 112
Size: 896 2455 248 702 1496 235
Fragments arranged by size:
2455 1496 896 702 248 235
BsaWI w'CCGG_w
Cuts at: 663 3221 3742 4420 4694 4841 5252 663
Size: 2558 521 678 274 147 411 1443
Fragments arranged by size:
2558 1443 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1129 1159 1129
Size: 30 6002
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1045 1215 1483 1519 1861 2293 2359 2809 3019
Size: 170 268 36 342 432 66 450 210
Cuts at: 3019 3022 1045
Size: 3 4055
Fragments arranged by size:
4055 450 432 342 268 210 170 66
36 3
BseYI C'CCAG_C
Cuts at: 212 381 892 1883 2073 2164 2597 2811 2829
Size: 169 511 991 190 91 433 214 18
Cuts at: 2829 2887 2993 3457 4215 4792 5288 212
Size: 58 106 464 758 577 496 956
Fragments arranged by size:
991 956 758 577 511 496 464 433
214 190 169 106 91 58 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2419 2351
Size: 68 5964
BsiEI CG_ry'CG
Cuts at: 104 155 2134 2976 2980 3574 3620 4828 104
Size: 51 1979 842 4 594 46 1208 1308
Fragments arranged by size:
1979 1308 1208 842 594 51 46 4
BsiHKAI G_wGCw'C
Cuts at: 629 727 1150 1601 1865 3046 3824 4014 4806
Size: 98 423 451 264 1181 778 190 792
Cuts at: 4806 629
Size: 1855
Fragments arranged by size:
1855 1181 792 778 451 423 264 190
98
BsmI GAATG_Cn'
Cuts at: 213 1675 213
Size: 1462 4570
BsmBI CGTCTCn'nnnn_
Cuts at: 297 297
Size: 6032
Bsp1286I G_dGCh'C
Cuts at: 233 368 565 629 727 748 848 1150 1601
Size: 135 197 64 98 21 100 302 451
Cuts at: 1601 1623 1865 2598 2654 2842 2888 3046 3657
Size: 22 242 733 56 188 46 158 611
Cuts at: 3657 3750 3824 4014 4076 4806 233
Size: 93 74 190 62 730 1459
Fragments arranged by size:
1459 733 730 611 451 302 242 197
190 188 158 135 100 98 93 74
64 62 56 46 22 21
BspEI T'CCGG_A
Cuts at: 663 5252 663
Size: 4589 1443
BspHI T'CATG_A
Cuts at: 5208 5208
Size: 6032
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 1152 2019 2114 2292 2345 2624 2680 3082
Size: 703 867 95 178 53 279 56 402
Cuts at: 3082 3296 3598 3979 449
Size: 214 302 381 2502
Fragments arranged by size:
2502 867 703 402 381 302 279 214
178 95 56 53
BsrBI CCG'CTC
Cuts at: 50 149 741 1257 1359 1809 2966 4324 4378
Size: 99 592 516 102 450 1157 1358 54
Cuts at: 4378 5862 6027 50
Size: 1484 165 55
Fragments arranged by size:
1484 1358 1157 592 516 450 165 102
99 55 54
BsrDI GCAATG_nn'
Cuts at: 3944 3944
Size: 6032
BsrFI r'CCGG_y
Cuts at: 1610 1964 2129 2975 4030 4211 4420 1610
Size: 354 165 846 1055 181 209 3222
Fragments arranged by size:
3222 1055 846 354 209 181 165
BsrGI T'GTAC_A
Cuts at: 2415 2415
Size: 6032
BssSI C'ACGA_G
Cuts at: 1834 4303 4661 1834
Size: 2469 358 3205
Fragments arranged by size:
3205 2469 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1167 2397 2625 2754 3409 1167
Size: 1230 228 129 655 3790
Fragments arranged by size:
3790 1230 655 228 129
BstBI TT'CG_AA
Cuts at: 1677 4393 1677
Size: 2716 3316
BstXI CCAn_nnnn'nTGG
Cuts at: 375 900 375
Size: 525 5507
BstYI r'GATC_y
Cuts at: 1 666 3057 3524 3547 3882 4128 5140 5226
Size: 665 2391 467 23 335 246 1012 86
Cuts at: 5226 5238 5584 1
Size: 12 346 449
Fragments arranged by size:
2391 1012 665 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2342 2342
Size: 6032
BtgI C'CryG_G
Cuts at: 107 2124 2285 2426 2876 107
Size: 2017 161 141 450 3263
Fragments arranged by size:
3263 2017 450 161 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3982 4163 4326 3982
Size: 181 163 5688
Fragments arranged by size:
5688 181 163
BtsI GCAGTG_nn'
Cuts at: 331 1148 1573 1793 2332 3275 331
Size: 817 425 220 539 943 3088
Fragments arranged by size:
3088 943 817 539 425 220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2224 2259 2224
Size: 35 5997
DraI TTT'AAA
Cuts at: 3031 3031
Size: 6032
DraIII CAC_nnn'GTG
Cuts at: 1215 1776 2184 2397 1215
Size: 561 408 213 4850
Fragments arranged by size:
4850 561 408 213
DrdI GACnn_nn'nnGTC
Cuts at: 3738 4596 3738
Size: 858 5174
EaeI y'GGCC_r
Cuts at: 152 502 1337 1538 1856 2127 2131 2229 2318
Size: 350 835 201 318 271 4 98 89
Cuts at: 2318 2718 2927 2977 3617 3791 4182 4209 5554
Size: 400 209 50 640 174 391 27 1345
Cuts at: 5554 152
Size: 630
Fragments arranged by size:
1345 835 640 630 400 391 350 318
271 209 201 174 98 89 50 27
4
EagI C'GGCC_G
Cuts at: 152 2131 2977 3617 152
Size: 1979 846 640 2567
Fragments arranged by size:
2567 1979 846 640
EarI CTCTTCn'nnn_
Cuts at: 796 1894 2399 2573 2713 4055 4265 796
Size: 1098 505 174 140 1342 210 2563
Fragments arranged by size:
2563 1342 1098 505 210 174 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 667 4240 4550 4696 5842 667
Size: 3573 310 146 1146 857
Fragments arranged by size:
3573 1146 857 310 146
EcoICRI GAG'CTC
Cuts at: 627 1599 3044 627
Size: 972 1445 3615
Fragments arranged by size:
3615 1445 972
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 279 1207 1225 1447 1510 2176 2398 2812
Size: 41 928 18 222 63 666 222 414
Cuts at: 2812 3857 4289 5036 5304 238
Size: 1045 432 747 268 966
Fragments arranged by size:
1045 966 928 747 666 432 414 268
222 222 63 41 18
EcoNI CCTnn'n_nnAGG
Cuts at: 322 2341 2746 322
Size: 2019 405 3608
Fragments arranged by size:
3608 2019 405
EcoO109I rG'GnC_Cy
Cuts at: 468 580 744 745 1063 1641 1851 2262 2884
Size: 112 164 1 318 578 210 411 622
Cuts at: 2884 3171 468
Size: 287 3329
Fragments arranged by size:
3329 622 578 411 318 287 210 164
112 1
EcoRI G'AATT_C
Cuts at: 3036 3036
Size: 6032
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 664 1689 4051 664
Size: 1025 2362 2645
Fragments arranged by size:
2645 2362 1025
FseI GG_CCGG'CC
Cuts at: 2133 2133
Size: 6032
FspI TGC'GCA
Cuts at: 2362 3813 2362
Size: 1451 4581
HaeII r_GCGC'y
Cuts at: 789 1011 3000 3466 3714 4736 5715 789
Size: 222 1989 466 248 1022 979 1106
Fragments arranged by size:
1989 1106 1022 979 466 248 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3874 3904 3906 3936 3874
Size: 30 2 30 5970
Fragments arranged by size:
5970 30 30 2
HincII GTy'rAC
Cuts at: 3071 3071
Size: 6032
KasI G'GCGC_C
Cuts at: 1007 3462 3710 1007
Size: 2455 248 3329
Fragments arranged by size:
3329 2455 248
KpnI G_GTAC'C
Cuts at: 3052 3052
Size: 6032
MluI A'CGCG_T
Cuts at: 5465 5465
Size: 6032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 526 4703 4887 526
Size: 4177 184 1671
Fragments arranged by size:
4177 1671 184
MscI TGG'CCA
Cuts at: 1339 1540 2231 2320 2720 3793 1339
Size: 201 691 89 400 1073 3578
Fragments arranged by size:
3578 1073 691 400 201 89
MslI CAynn'nnrTG
Cuts at: 720 4021 4148 720
Size: 3301 127 2604
Fragments arranged by size:
3301 2604 127
MspA1I CmG'CkG
Cuts at: 216 306 528 1203 1239 2568 2932 3143 3457
Size: 90 222 675 36 1329 364 211 314
Cuts at: 3457 3817 4830 5075 216
Size: 360 1013 245 1173
Fragments arranged by size:
1329 1173 1013 675 364 360 314 245
222 211 90 36
NaeI GCC'GGC
Cuts at: 1612 1966 2131 4213 1612
Size: 354 165 2082 3431
Fragments arranged by size:
3431 2082 354 165
NarI GG'CG_CC
Cuts at: 1008 3463 3711 1008
Size: 2455 248 3329
Fragments arranged by size:
3329 2455 248
NcoI C'CATG_G
Cuts at: 107 2426 107
Size: 2319 3713
NdeI CA'TA_TG
Cuts at: 2723 2723
Size: 6032
NgoMIV G'CCGG_C
Cuts at: 1610 1964 2129 4211 1610
Size: 354 165 2082 3431
Fragments arranged by size:
3431 2082 354 165
NheI G'CTAG_C
Cuts at: 2440 2440
Size: 6032
NspI r_CATG'y
Cuts at: 3085 3585 4492 3085
Size: 500 907 4625
Fragments arranged by size:
4625 907 500
PasI CC'CwG_GG
Cuts at: 831 831
Size: 6032
PciI A'CATG_T
Cuts at: 4488 4488
Size: 6032
PfoI T'CCnGG_A
Cuts at: 119 1184 1487 2555 119
Size: 1065 303 1068 3596
Fragments arranged by size:
3596 1068 1065 303
PmeI GTTT'AAAC
Cuts at: 3031 3031
Size: 6032
PmlI CAC'GTG
Cuts at: 375 1278 2394 375
Size: 903 1116 4013
Fragments arranged by size:
4013 1116 903
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5171 5203 5171
Size: 32 6000
PpuMI rG'GwC_Cy
Cuts at: 468 2262 468
Size: 1794 4238
PspOMI G'GGCC_C
Cuts at: 744 2884 744
Size: 2140 3892
PstI C_TGCA'G
Cuts at: 1529 1628 2303 2527 2567 2621 2750 2804 3079
Size: 99 675 224 40 54 129 54 275
Cuts at: 3079 3764 1529
Size: 685 3797
Fragments arranged by size:
3797 685 675 275 224 129 99 54
54 40
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6032
PvuII CAG'CTG
Cuts at: 216 306 528 1239 3457 3817 216
Size: 90 222 711 2218 360 2431
Fragments arranged by size:
2431 2218 711 360 222 90
RsrII CG'GwC_CG
Cuts at: 4227 4227
Size: 6032
SacI G_AGCT'C
Cuts at: 629 1601 3046 629
Size: 972 1445 3615
Fragments arranged by size:
3615 1445 972
SalI G'TCGA_C
Cuts at: 3069 3069
Size: 6032
SapI GCTCTTCn'nnn_
Cuts at: 1894 4055 4265 1894
Size: 2161 210 3661
Fragments arranged by size:
3661 2161 210
SbfI CC_TGCA'GG
Cuts at: 2303 2621 3079 2303
Size: 318 458 5256
Fragments arranged by size:
5256 458 318
ScaI AGT'ACT
Cuts at: 5549 5549
Size: 6032
SfcI C'TryA_G
Cuts at: 33 906 1525 1624 2004 2299 2523 2563 2585
Size: 873 619 99 380 295 224 40 22
Cuts at: 2585 2617 2746 2800 3075 3760 4753 4944 33
Size: 32 129 54 275 685 993 191 1121
Fragments arranged by size:
1121 993 873 685 619 380 295 275
224 191 129 99 54 40 32 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 1393 2857 1393
Size: 1464 4568
SfoI GGC'GCC
Cuts at: 1009 3464 3712 1009
Size: 2455 248 3329
Fragments arranged by size:
3329 2455 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6032
SmaI CCC'GGG
Cuts at: 952 1390 2848 3054 952
Size: 438 1458 206 3930
Fragments arranged by size:
3930 1458 438 206
SmlI C'TyrA_G
Cuts at: 730 1480 2197 2455 2533 2902 3187 4102 4594
Size: 750 717 258 78 369 285 915 492
Cuts at: 4594 4856 730
Size: 262 1906
Fragments arranged by size:
1906 915 750 717 492 369 285 262
258 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6032
SphI G_CATG'C
Cuts at: 3085 3585 3085
Size: 500 5532
SrfI GCCC'GGGC
Cuts at: 2848 2848
Size: 6032
StuI AGG'CCT
Cuts at: 1840 2539 1840
Size: 699 5333
StyI C'CwwG_G
Cuts at: 107 603 1664 2426 2834 2852 3166 3520 107
Size: 496 1061 762 408 18 314 354 2619
Fragments arranged by size:
2619 1061 762 496 408 354 314 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 884 1920 3615 3748 4207 4416 884
Size: 1036 1695 133 459 209 2500
Fragments arranged by size:
2500 1695 1036 459 209 133
TatI w'GTAC_w
Cuts at: 1778 2415 2942 5547 1778
Size: 637 527 2605 2263
Fragments arranged by size:
2605 2263 637 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 863 895 1129 1161 5549 5581 863
Size: 32 234 32 4388 32 1314
Fragments arranged by size:
4388 1314 234 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1378 3829 1378
Size: 2451 3581
XbaI T'CTAG_A
Cuts at: 3063 3063
Size: 6032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 512 2049 2433 3527 512
Size: 1537 384 1094 3017
Fragments arranged by size:
3017 1537 1094 384
XmaI C'CCGG_G
Cuts at: 950 1388 2846 3052 950
Size: 438 1458 206 3930
Fragments arranged by size:
3930 1458 438 206
XmnI GAAnn'nnTTC
Cuts at: 1327 1673 1327
Size: 346 5686
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AscI AvrII BaeI BcgI BfrBI BmgBI
BsiWI BssHII BstEII BstZ17I ClaI EcoRV FspAI HindIII
HpaI MfeI NotI NruI NsiI PacI PflMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SspI SwaI XhoI ZraI