Restriction map for
pF1KA1464
(Circular) (Six-base) MAPSORT of: pF1KA1464.seq Check: 1262 from: 1 to: 4973
pF1KA1464 4973 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 652 652
Size: 4973
AccI GT'mk_AC
Cuts at: 2011 2011
Size: 4973
Acc65I G'GTAC_C
Cuts at: 1989 1989
Size: 4973
AclI AA'CG_TT
Cuts at: 4813 4813
Size: 4973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1719 2798 3230 3583 4245 1719
Size: 1079 432 353 662 2447
Fragments arranged by size:
2447 1079 662 432 353
AfeI AGC'GCT
Cuts at: 1054 1054
Size: 4973
AflIII A'CryG_T
Cuts at: 2325 4125 4406 2325
Size: 1800 281 2892
Fragments arranged by size:
2892 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 210 231 1008 1851 2278 3716 210
Size: 21 777 843 427 1438 1467
Fragments arranged by size:
1467 1438 843 777 427 21
ApaLI G'TGCA_C
Cuts at: 3811 3811
Size: 4973
ApoI r'AATT_y
Cuts at: 1701 1977 4326 4582 1701
Size: 276 2349 256 2092
Fragments arranged by size:
2349 2092 276 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4973
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
AvaI C'yCGr_G
Cuts at: 129 564 1916 1993 129
Size: 435 1352 77 3109
Fragments arranged by size:
3109 1352 435 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1422 1455 1422
Size: 33 4940
BamHI G'GATC_C
Cuts at: 1998 1998
Size: 4973
BanI G'GyrC_C
Cuts at: 640 1537 1989 2403 2651 2686 640
Size: 897 452 414 248 35 2927
Fragments arranged by size:
2927 897 452 414 248 35
BanII G_rGCy'C
Cuts at: 283 1165 1987 3017 283
Size: 882 822 1030 2239
Fragments arranged by size:
2239 1030 882 822
BbeI G_GCGC'C
Cuts at: 2407 2655 2407
Size: 248 4725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1266 1300 1266
Size: 34 4939
BciVI GTATCCnnnnn_n'
Cuts at: 245 2871 3927 4973 245
Size: 2626 1056 1046 245
Fragments arranged by size:
2626 1056 1046 245
BclI T'GATC_A
Cuts at: 2493 2493
Size: 4973
BglI GCCn_nnn'nGGC
Cuts at: 112 1028 1775 112
Size: 916 747 3310
Fragments arranged by size:
3310 916 747
BglII A'GATC_T
Cuts at: 2488 4525 2488
Size: 2037 2936
BlpI GC'TnA_GC
Cuts at: 211 2085 211
Size: 1874 3099
Bme1580I G_kGCm'C
Cuts at: 826 852 2598 2691 3815 826
Size: 26 1746 93 1124 1984
Fragments arranged by size:
1984 1746 1124 93 26
BmgBI CAC'GTC
Cuts at: 609 609
Size: 4973
BmrI ACTGGGnnnn_n'
Cuts at: 1223 1765 2185 2266 2601 4745 1223
Size: 542 420 81 335 2144 1451
Fragments arranged by size:
2144 1451 542 420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1419 3340 1419
Size: 1921 3052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 1036 1093 1782 297
Size: 739 57 689 3488
Fragments arranged by size:
3488 739 689 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 1087 2149 3028 3742 4040 464
Size: 623 1062 879 714 298 1397
Fragments arranged by size:
1397 1062 879 714 623 298
BsaI GGTCTCn'nnnn_
Cuts at: 313 1036 1447 4677 313
Size: 723 411 3230 609
Fragments arranged by size:
3230 723 609 411
BsaAI yAC'GTr
Cuts at: 2326 2326
Size: 4973
BsaBI GATnn'nnATC
Cuts at: 6 2487 4524 6
Size: 2481 2037 455
Fragments arranged by size:
2481 2037 455
BsaHI Gr'CG_yC
Cuts at: 485 749 976 1283 2404 2652 3354 4850 485
Size: 264 227 307 1121 248 702 1496 608
Fragments arranged by size:
1496 1121 702 608 307 264 248 227
BsaWI w'CCGG_w
Cuts at: 2162 2683 3361 3772 3919 4193 2162
Size: 521 678 411 147 274 2942
Fragments arranged by size:
2942 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1183 1213 1183
Size: 30 4943
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 552 1081 1326 1341 1344 1347 1350 150
Size: 402 529 245 15 3 3 3 3773
Fragments arranged by size:
3773 529 402 245 15 3 3 3
BseYI C'CCAG_C
Cuts at: 136 175 1049 1204 1267 1330 1424 2398 3156
Size: 39 874 155 63 63 94 974 758
Cuts at: 3156 3821 4229 136
Size: 665 408 880
Fragments arranged by size:
974 880 874 758 665 408 155 94
63 63 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 1310
Size: 4973
BsiEI CG_ry'CG
Cuts at: 104 2515 2561 3791 104
Size: 2411 46 1230 1286
Fragments arranged by size:
2411 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1079 1165 1490 1987 2765 2955 3815 1079
Size: 86 325 497 778 190 860 2237
Fragments arranged by size:
2237 860 778 497 325 190 86
BsmI GAATG_Cn'
Cuts at: 549 549
Size: 4973
BsmBI CGTCTCn'nnnn_
Cuts at: 599 599
Size: 4973
Bsp1286I G_dGCh'C
Cuts at: 283 826 852 1079 1165 1490 1987 2598 2691
Size: 543 26 227 86 325 497 611 93
Cuts at: 2691 2765 2955 3017 3815 283
Size: 74 190 62 798 1441
Fragments arranged by size:
1441 798 611 543 497 325 227 190
93 86 74 62 26
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 405 652 1565 1859 2023 2237 2539 2920 405
Size: 247 913 294 164 214 302 381 2458
Fragments arranged by size:
2458 913 381 302 294 247 214 164
BsrBI CCG'CTC
Cuts at: 50 3265 3319 4803 4968 50
Size: 3215 54 1484 165 55
Fragments arranged by size:
3215 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1284 2885 1284
Size: 1601 3372
BsrFI r'CCGG_y
Cuts at: 215 1109 2971 3152 215
Size: 894 1862 181 2036
Fragments arranged by size:
2036 1862 894 181
BssSI C'ACGA_G
Cuts at: 901 3244 3952 901
Size: 2343 708 1922
Fragments arranged by size:
2343 1922 708
BstAPI GCAn_nnn'nTGC
Cuts at: 873 2350 873
Size: 1477 3496
BstBI TT'CG_AA
Cuts at: 3334 3334
Size: 4973
BstEII G'GTnAC_C
Cuts at: 529 529
Size: 4973
BstXI CCAn_nnnn'nTGG
Cuts at: 583 673 583
Size: 90 4883
BstYI r'GATC_y
Cuts at: 1 998 1998 2465 2488 2823 3069 3375 3387
Size: 997 1000 467 23 335 246 306 12
Cuts at: 3387 3473 4525 1
Size: 86 1052 449
Fragments arranged by size:
1052 1000 997 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 239 534 929 107
Size: 132 295 395 4151
Fragments arranged by size:
4151 395 295 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2923 3104 3267 2923
Size: 181 163 4629
Fragments arranged by size:
4629 181 163
BtsI GCAGTG_nn'
Cuts at: 831 878 2216 831
Size: 47 1338 3588
Fragments arranged by size:
3588 1338 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 918 953 1509 1544 918
Size: 35 556 35 4347
Fragments arranged by size:
4347 556 35 35
DraI TTT'AAA
Cuts at: 1972 1972
Size: 4973
DrdI GACnn_nn'nnGTC
Cuts at: 1147 1569 2679 4023 1147
Size: 422 1110 1344 2097
Fragments arranged by size:
2097 1344 1110 422
EaeI y'GGCC_r
Cuts at: 573 663 698 1650 1776 2558 2732 3123 3150
Size: 90 35 952 126 782 174 391 27
Cuts at: 3150 4495 573
Size: 1345 1051
Fragments arranged by size:
1345 1051 952 782 391 174 126 90
35 27
EagI C'GGCC_G
Cuts at: 2558 2558
Size: 4973
EarI CTCTTCn'nnn_
Cuts at: 441 1099 1374 2996 3206 441
Size: 658 275 1622 210 2208
Fragments arranged by size:
2208 1622 658 275 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3181 3923 4069 4783 3181
Size: 742 146 714 3371
Fragments arranged by size:
3371 742 714 146
EcoICRI GAG'CTC
Cuts at: 1163 1985 1163
Size: 822 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 1036 1093 1719 1782 2798 3230 3583 4245
Size: 739 57 626 63 1016 432 353 662
Cuts at: 4245 297
Size: 1025
Fragments arranged by size:
1025 1016 739 662 626 432 353 63
57
EcoNI CCTnn'n_nnAGG
Cuts at: 1885 1885
Size: 4973
EcoO109I rG'GnC_Cy
Cuts at: 1554 2112 1554
Size: 558 4415
EcoRI G'AATT_C
Cuts at: 1977 1977
Size: 4973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4973
FspI TGC'GCA
Cuts at: 2754 2754
Size: 4973
HaeII r_GCGC'y
Cuts at: 176 222 231 1056 1832 2407 2655 3885 4656
Size: 46 9 825 776 575 248 1230 771
Cuts at: 4656 176
Size: 493
Fragments arranged by size:
1230 825 776 771 575 493 248 46
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 596 628 983 1015 2815 2845 2847
Size: 32 209 32 355 32 1800 30 2
Cuts at: 2847 2877 355
Size: 30 2451
Fragments arranged by size:
2451 1800 355 209 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 246 489 627 2012 246
Size: 243 138 1385 3207
Fragments arranged by size:
3207 1385 243 138
KasI G'GCGC_C
Cuts at: 2403 2651 2403
Size: 248 4725
KpnI G_GTAC'C
Cuts at: 1993 1993
Size: 4973
MluI A'CGCG_T
Cuts at: 4406 4406
Size: 4973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1424 3732 3916 1424
Size: 2308 184 2481
Fragments arranged by size:
2481 2308 184
MscI TGG'CCA
Cuts at: 575 665 1778 2734 575
Size: 90 1113 956 2814
Fragments arranged by size:
2814 1113 956 90
MslI CAynn'nnrTG
Cuts at: 362 524 539 1717 1896 2962 3089 362
Size: 162 15 1178 179 1066 127 2246
Fragments arranged by size:
2246 1178 1066 179 162 127 15
MspA1I CmG'CkG
Cuts at: 241 264 1271 2084 2398 2758 3542 3787 241
Size: 23 1007 813 314 360 784 245 1427
Fragments arranged by size:
1427 1007 813 784 360 314 245 23
NaeI GCC'GGC
Cuts at: 217 1111 3154 217
Size: 894 2043 2036
Fragments arranged by size:
2043 2036 894
NarI GG'CG_CC
Cuts at: 2404 2652 2404
Size: 248 4725
NcoI C'CATG_G
Cuts at: 107 534 107
Size: 427 4546
NgoMIV G'CCGG_C
Cuts at: 215 1109 3152 215
Size: 894 2043 2036
Fragments arranged by size:
2043 2036 894
NspI r_CATG'y
Cuts at: 798 1245 1518 2026 2526 4129 798
Size: 447 273 508 500 1603 1642
Fragments arranged by size:
1642 1603 508 500 447 273
PasI CC'CwG_GG
Cuts at: 196 315 1063 1881 196
Size: 119 748 818 3288
Fragments arranged by size:
3288 818 748 119
PciI A'CATG_T
Cuts at: 4125 4125
Size: 4973
PflMI CCAn_nnn'nTGG
Cuts at: 1008 1008
Size: 4973
PfoI T'CCnGG_A
Cuts at: 274 274
Size: 4973
PmeI GTTT'AAAC
Cuts at: 1972 1972
Size: 4973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4941
PpuMI rG'GwC_Cy
Cuts at: 1554 1554
Size: 4973
PstI C_TGCA'G
Cuts at: 728 881 1562 1727 2020 2705 728
Size: 153 681 165 293 685 2996
Fragments arranged by size:
2996 685 681 293 165 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4973
PvuII CAG'CTG
Cuts at: 1271 2398 2758 1271
Size: 1127 360 3486
Fragments arranged by size:
3486 1127 360
RsrII CG'GwC_CG
Cuts at: 3168 3168
Size: 4973
SacI G_AGCT'C
Cuts at: 1165 1987 1165
Size: 822 4151
SacII CC_GC'GG
Cuts at: 242 242
Size: 4973
SalI G'TCGA_C
Cuts at: 2010 2010
Size: 4973
SapI GCTCTTCn'nnn_
Cuts at: 2996 3206 2996
Size: 210 4763
SbfI CC_TGCA'GG
Cuts at: 2020 2020
Size: 4973
ScaI AGT'ACT
Cuts at: 4490 4490
Size: 4973
SfcI C'TryA_G
Cuts at: 33 724 877 1272 1558 1723 2016 2701 3669
Size: 691 153 395 286 165 293 685 968
Cuts at: 3669 3860 33
Size: 191 1146
Fragments arranged by size:
1146 968 691 685 395 293 286 191
165 153
SfoI GGC'GCC
Cuts at: 2405 2653 2405
Size: 248 4725
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
SmaI CCC'GGG
Cuts at: 131 1995 131
Size: 1864 3109
SmlI C'TyrA_G
Cuts at: 479 1102 2128 3043 3757 4019 479
Size: 623 1026 915 714 262 1433
Fragments arranged by size:
1433 1026 915 714 623 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4973
SphI G_CATG'C
Cuts at: 2026 2526 2026
Size: 500 4473
StuI AGG'CCT
Cuts at: 722 1925 722
Size: 1203 3770
StyI C'CwwG_G
Cuts at: 107 284 534 672 812 2107 2461 107
Size: 177 250 138 140 1295 354 2619
Fragments arranged by size:
2619 1295 354 250 177 140 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1063 1344 2556 2689 3148 3357 400
Size: 663 281 1212 133 459 209 2016
Fragments arranged by size:
2016 1212 663 459 281 209 133
TatI w'GTAC_w
Cuts at: 4488 4488
Size: 4973
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1128 1160 1795 1827 4490 4522 1128
Size: 32 635 32 2663 32 1579
Fragments arranged by size:
2663 1579 635 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2770 2770
Size: 4973
XbaI T'CTAG_A
Cuts at: 2004 2004
Size: 4973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 541 2468 541
Size: 1927 3046
XmaI C'CCGG_G
Cuts at: 129 1993 129
Size: 1864 3109
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BfrBI BmtI Bpu10I BsiWI
BspEI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI