Restriction map for
pF1KA1460
(Circular) (Six-base) MAPSORT of: pF1KA1460.seq Check: 1938 from: 1 to: 8999
pF1KA1460 8999 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 4440 5341 6037 4440
Size: 901 696 7402
Fragments arranged by size:
7402 901 696
Acc65I G'GTAC_C
Cuts at: 1360 6015 1360
Size: 4655 4344
AclI AA'CG_TT
Cuts at: 5153 8839 5153
Size: 3686 5313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 842 908 1360 1415 3107 3326 4267 5168
Size: 144 66 452 55 1692 219 941 901
Cuts at: 5168 6824 7256 7609 8271 698
Size: 1656 432 353 662 1426
Fragments arranged by size:
1692 1656 1426 941 901 662 452 432
353 219 144 66 55
AflIII A'CryG_T
Cuts at: 1075 2253 3167 5618 6351 8151 8432 1075
Size: 1178 914 2451 733 1800 281 1642
Fragments arranged by size:
2451 1800 1642 1178 914 733 281
AleI CACnn'nnGTG
Cuts at: 3325 5621 5972 3325
Size: 2296 351 6352
Fragments arranged by size:
6352 2296 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2318 2318
Size: 8999
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1254 1286 4303 4335 1254
Size: 32 3017 32 5918
Fragments arranged by size:
5918 3017 32 32
AlwNI CAG_nnn'CTG
Cuts at: 835 5703 6304 7742 835
Size: 4868 601 1438 2092
Fragments arranged by size:
4868 2092 1438 601
ApaLI G'TGCA_C
Cuts at: 4022 7837 4022
Size: 3815 5184
ApoI r'AATT_y
Cuts at: 732 1191 1884 4077 4939 5385 6003 8352 8608
Size: 459 693 2193 862 446 618 2349 256
Cuts at: 8608 732
Size: 1123
Fragments arranged by size:
2349 2193 1123 862 693 618 459 446
256
AseI AT'TA_AT
Cuts at: 20 1280 5036 20
Size: 1260 3756 3983
Fragments arranged by size:
3983 3756 1260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8999
AvaI C'yCGr_G
Cuts at: 4139 5731 5918 6019 4139
Size: 1592 187 101 7119
Fragments arranged by size:
7119 1592 187 101
BamHI G'GATC_C
Cuts at: 718 6024 718
Size: 5306 3693
BanI G'GyrC_C
Cuts at: 1210 1360 1675 6015 6429 6677 6712 1210
Size: 150 315 4340 414 248 35 3497
Fragments arranged by size:
4340 3497 414 315 248 150 35
BanII G_rGCy'C
Cuts at: 3296 3424 5758 6013 7043 3296
Size: 128 2334 255 1030 5252
Fragments arranged by size:
5252 2334 1030 255 128
BbeI G_GCGC'C
Cuts at: 6433 6681 6433
Size: 248 8751
BbsI GAAGACnn'nnnn_
Cuts at: 746 3905 746
Size: 3159 5840
BbvCI CC'TCA_GC
Cuts at: 305 828 5161 305
Size: 523 4333 4143
Fragments arranged by size:
4333 4143 523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4459 4493 4459
Size: 34 8965
BciVI GTATCCnnnnn_n'
Cuts at: 512 2304 6897 7953 8999 512
Size: 1792 4593 1056 1046 512
Fragments arranged by size:
4593 1792 1056 1046 512
BclI T'GATC_A
Cuts at: 2202 5528 6519 2202
Size: 3326 991 4682
Fragments arranged by size:
4682 3326 991
BglI GCCn_nnn'nGGC
Cuts at: 1721 3524 5753 1721
Size: 1803 2229 4967
Fragments arranged by size:
4967 2229 1803
BglII A'GATC_T
Cuts at: 4343 6514 8551 4343
Size: 2171 2037 4791
Fragments arranged by size:
4791 2171 2037
BlpI GC'TnA_GC
Cuts at: 6111 6111
Size: 8999
Bme1580I G_kGCm'C
Cuts at: 1147 1789 4026 6624 6717 7841 1147
Size: 642 2237 2598 93 1124 2305
Fragments arranged by size:
2598 2305 2237 1124 642 93
BmrI ACTGGGnnnn_n'
Cuts at: 284 1046 2177 2685 4097 4344 5638 6211 6292
Size: 762 1131 508 1412 247 1294 573 81
Cuts at: 6292 6627 8771 284
Size: 335 2144 512
Fragments arranged by size:
2144 1412 1294 1131 762 573 512 508
335 247 81
BmtI G_CTAG'C
Cuts at: 1202 1202
Size: 8999
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3606 5448 7366 3606
Size: 1842 1918 5239
Fragments arranged by size:
5239 1918 1842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 1718 1910 2921 3299 3408 3818 4142 4752
Size: 848 192 1011 378 109 410 324 610
Cuts at: 4752 4850 5766 870
Size: 98 916 4103
Fragments arranged by size:
4103 1011 916 848 610 410 378 324
192 109 98
Bpu10I CC'TnA_GC
Cuts at: 305 828 1627 2730 4595 5161 5433 305
Size: 523 799 1103 1865 566 272 3871
Fragments arranged by size:
3871 1865 1103 799 566 523 272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3563 4184 6175 7054 7768 8066 2173
Size: 1390 621 1991 879 714 298 3106
Fragments arranged by size:
3106 1991 1390 879 714 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 1089 2870 5222 5834 8703 1089
Size: 1781 2352 612 2869 1385
Fragments arranged by size:
2869 2352 1781 1385 612
BsaAI yAC'GTr
Cuts at: 2254 3416 4894 5345 6352 2254
Size: 1162 1478 451 1007 4901
Fragments arranged by size:
4901 1478 1162 1007 451
BsaBI GATnn'nnATC
Cuts at: 6 2031 3860 5798 6513 8550 6
Size: 2025 1829 1938 715 2037 455
Fragments arranged by size:
2037 2025 1938 1829 715 455
BsaHI Gr'CG_yC
Cuts at: 2148 5393 6430 6678 7380 8876 2148
Size: 3245 1037 248 702 1496 2271
Fragments arranged by size:
3245 2271 1496 1037 702 248
BsaWI w'CCGG_w
Cuts at: 846 1507 2761 6188 6709 7387 7798 7945 8219
Size: 661 1254 3427 521 678 411 147 274
Cuts at: 8219 846
Size: 1626
Fragments arranged by size:
3427 1626 1254 678 661 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1254 1284 1808 1838 2099 2129 5202 5232 5697
Size: 30 524 30 261 30 3073 30 465
Cuts at: 5697 5727 5764 5794 1254
Size: 30 37 30 4459
Fragments arranged by size:
4459 3073 524 465 261 37 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2593 4199 5683 2593
Size: 1606 1484 5909
Fragments arranged by size:
5909 1606 1484
BseYI C'CCAG_C
Cuts at: 737 1945 3018 3039 3065 3284 3373 3377 3527
Size: 1208 1073 21 26 219 89 4 150
Cuts at: 3527 4059 4322 5417 5751 5815 6424 7182 7847
Size: 532 263 1095 334 64 609 758 665
Cuts at: 7847 8255 737
Size: 408 1481
Fragments arranged by size:
1481 1208 1095 1073 758 665 609 532
408 334 263 219 150 89 64 26
21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1311 1537 1859 1904 4474 5599 694
Size: 617 226 322 45 2570 1125 4094
Fragments arranged by size:
4094 2570 1125 617 322 226 45
BsiEI CG_ry'CG
Cuts at: 104 3872 4420 6541 6587 7817 104
Size: 3768 548 2121 46 1230 1286
Fragments arranged by size:
3768 2121 1286 1230 548 46
BsiHKAI G_wGCw'C
Cuts at: 1567 4026 4200 6013 6791 6981 7841 1567
Size: 2459 174 1813 778 190 860 2725
Fragments arranged by size:
2725 2459 1813 860 778 190 174
BsmI GAATG_Cn'
Cuts at: 792 1140 1752 1980 792
Size: 348 612 228 7811
Fragments arranged by size:
7811 612 348 228
Bsp1286I G_dGCh'C
Cuts at: 1147 1567 1789 3296 3424 4026 4200 5758 6013
Size: 420 222 1507 128 602 174 1558 255
Cuts at: 6013 6624 6717 6791 6981 7043 7841 1147
Size: 611 93 74 190 62 798 2305
Fragments arranged by size:
2305 1558 1507 798 611 602 420 255
222 190 174 128 93 74 62
BspEI T'CCGG_A
Cuts at: 846 846
Size: 8999
BspHI T'CATG_A
Cuts at: 5250 7431 5250
Size: 2181 6818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2478 6049 6263 6565 6946 2478
Size: 3571 214 302 381 4531
Fragments arranged by size:
4531 3571 381 302 214
BsrBI CCG'CTC
Cuts at: 50 7291 7345 8829 8994 50
Size: 7241 54 1484 165 55
Fragments arranged by size:
7241 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1638 2010 3389 3502 4240 6911 1638
Size: 372 1379 113 738 2671 3726
Fragments arranged by size:
3726 2671 1379 738 372 113
BsrFI r'CCGG_y
Cuts at: 4415 5747 6997 7178 4415
Size: 1332 1250 181 6236
Fragments arranged by size:
6236 1332 1250 181
BsrGI T'GTAC_A
Cuts at: 3771 3771
Size: 8999
BssSI C'ACGA_G
Cuts at: 506 7270 7978 506
Size: 6764 708 1527
Fragments arranged by size:
6764 1527 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4467 5507 6376 4467
Size: 1040 869 7090
Fragments arranged by size:
7090 1040 869
BstBI TT'CG_AA
Cuts at: 7360 7360
Size: 8999
BstXI CCAn_nnnn'nTGG
Cuts at: 1732 3327 4042 5974 1732
Size: 1595 715 1932 4757
Fragments arranged by size:
4757 1932 1595 715
BstYI r'GATC_y
Cuts at: 1 595 718 2247 2968 3135 4343 6024 6491
Size: 594 123 1529 721 167 1208 1681 467
Cuts at: 6491 6514 6849 7095 7401 7413 7499 8551 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1681 1529 1208 1052 721 594 467 449
335 306 246 167 123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 4226 5062 4226
Size: 836 8163
BtgI C'CryG_G
Cuts at: 1023 1042 1056 1206 1356 3917 4957 5990 1023
Size: 19 14 150 150 2561 1040 1033 4032
Fragments arranged by size:
4032 2561 1040 1033 150 150 19 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2138 6949 7130 7293 2138
Size: 4811 181 163 3844
Fragments arranged by size:
4811 3844 181 163
BtsI GCAGTG_nn'
Cuts at: 639 846 2094 3279 4926 5019 5288 6242 639
Size: 207 1248 1185 1647 93 269 954 3396
Fragments arranged by size:
3396 1647 1248 1185 954 269 207 93
ClaI AT'CG_AT
Cuts at: 5483 5483
Size: 8999
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 473 508 1800 1835 473
Size: 35 1292 35 7637
Fragments arranged by size:
7637 1292 35 35
DraI TTT'AAA
Cuts at: 3599 5998 3599
Size: 2399 6600
DraIII CAC_nnn'GTG
Cuts at: 5507 5507
Size: 8999
DrdI GACnn_nn'nnGTC
Cuts at: 4999 5321 6705 8049 4999
Size: 322 1384 1344 5949
Fragments arranged by size:
5949 1384 1344 322
EaeI y'GGCC_r
Cuts at: 1216 1722 3604 4417 4888 4930 6584 6758 7149
Size: 506 1882 813 471 42 1654 174 391
Cuts at: 7149 7176 8521 1216
Size: 27 1345 1694
Fragments arranged by size:
1882 1694 1654 1345 813 506 471 391
174 42 27
EagI C'GGCC_G
Cuts at: 4417 6584 4417
Size: 2167 6832
EarI CTCTTCn'nnn_
Cuts at: 1896 2039 3527 3533 4802 5582 5660 7022 7232
Size: 143 1488 6 1269 780 78 1362 210
Cuts at: 7232 1896
Size: 3663
Fragments arranged by size:
3663 1488 1362 1269 780 210 143 78
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7207 7949 8095 8809 7207
Size: 742 146 714 7397
Fragments arranged by size:
7397 742 714 146
EcoICRI GAG'CTC
Cuts at: 6011 6011
Size: 8999
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 842 870 908 1360 1415 1718 1910 2921
Size: 144 28 38 452 55 303 192 1011
Cuts at: 2921 3107 3299 3326 3408 3818 4142 4267 4752
Size: 186 192 27 82 410 324 125 485
Cuts at: 4752 4850 5168 5766 6824 7256 7609 8271 698
Size: 98 318 598 1058 432 353 662 1426
Fragments arranged by size:
1426 1058 1011 662 598 485 452 432
410 353 324 318 303 192 192 186
144 125 98 82 55 38 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3008 3008
Size: 8999
EcoO109I rG'GnC_Cy
Cuts at: 745 1252 1550 1714 3025 3880 4038 5265 5867
Size: 507 298 164 1311 855 158 1227 602
Cuts at: 5867 5896 6138 745
Size: 29 242 3606
Fragments arranged by size:
3606 1311 1227 855 602 507 298 242
164 158 29
EcoRI G'AATT_C
Cuts at: 1191 4077 6003 1191
Size: 2886 1926 4187
Fragments arranged by size:
4187 2886 1926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 653 970 4421 5352 7018 653
Size: 317 3451 931 1666 2634
Fragments arranged by size:
3451 2634 1666 931 317
FspI TGC'GCA
Cuts at: 5500 6780 5500
Size: 1280 7719
HaeII r_GCGC'y
Cuts at: 6433 6681 7911 8682 6433
Size: 248 1230 771 6750
Fragments arranged by size:
6750 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 868 900 1575 1607 2425 2457 3061 3093 4687
Size: 32 675 32 818 32 604 32 1594
Cuts at: 4687 4719 4750 4782 5202 5234 5697 5729 6841
Size: 32 31 32 420 32 463 32 1112
Cuts at: 6841 6871 6873 6903 868
Size: 30 2 30 2964
Fragments arranged by size:
2964 1594 1112 818 675 604 463 420
32 32 32 32 32 32 32 32
31 30 30 2
HincII GTy'rAC
Cuts at: 1432 1803 4441 4483 5055 5964 6038 1432
Size: 371 2638 42 572 909 74 4393
Fragments arranged by size:
4393 2638 909 572 371 74 42
HindIII A'AGCT_T
Cuts at: 4003 4003
Size: 8999
HpaI GTT'AAC
Cuts at: 1803 1803
Size: 8999
KasI G'GCGC_C
Cuts at: 6429 6677 6429
Size: 248 8751
KpnI G_GTAC'C
Cuts at: 1364 6019 1364
Size: 4655 4344
MluI A'CGCG_T
Cuts at: 8432 8432
Size: 8999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 788 2288 2746 2946 4098 5168 7758 7942 788
Size: 1500 458 200 1152 1070 2590 184 1845
Fragments arranged by size:
2590 1845 1500 1152 1070 458 200 184
MscI TGG'CCA
Cuts at: 1218 1724 3606 4890 4932 6760 1218
Size: 506 1882 1284 42 1828 3457
Fragments arranged by size:
3457 1882 1828 1284 506 42
MslI CAynn'nnrTG
Cuts at: 916 1453 1985 2705 2832 3325 3414 4741 5558
Size: 537 532 720 127 493 89 1327 817
Cuts at: 5558 5621 5807 5972 6988 7115 916
Size: 63 186 165 1016 127 2800
Fragments arranged by size:
2800 1327 1016 817 720 537 532 493
186 165 127 127 89 63
MspA1I CmG'CkG
Cuts at: 741 3231 3377 5417 5526 6110 6424 6784 7568
Size: 2490 146 2040 109 584 314 360 784
Cuts at: 7568 7813 741
Size: 245 1927
Fragments arranged by size:
2490 2040 1927 784 584 360 314 245
146 109
NaeI GCC'GGC
Cuts at: 5749 7180 5749
Size: 1431 7568
NarI GG'CG_CC
Cuts at: 6430 6678 6430
Size: 248 8751
NcoI C'CATG_G
Cuts at: 1023 1042 1056 1206 1356 3917 4957 5990 1023
Size: 19 14 150 150 2561 1040 1033 4032
Fragments arranged by size:
4032 2561 1040 1033 150 150 19 14
NgoMIV G'CCGG_C
Cuts at: 5747 7178 5747
Size: 1431 7568
NheI G'CTAG_C
Cuts at: 1198 1198
Size: 8999
NspI r_CATG'y
Cuts at: 1079 2405 3171 3978 4149 5514 5622 6052 6552
Size: 1326 766 807 171 1365 108 430 500
Cuts at: 6552 8155 1079
Size: 1603 1923
Fragments arranged by size:
1923 1603 1365 1326 807 766 500 430
171 108
PasI CC'CwG_GG
Cuts at: 2865 3296 2865
Size: 431 8568
PciI A'CATG_T
Cuts at: 1075 3167 5618 8151 1075
Size: 2092 2451 2533 1923
Fragments arranged by size:
2533 2451 2092 1923
PflMI CCAn_nnn'nTGG
Cuts at: 3065 3508 3527 4285 3065
Size: 443 19 758 7779
Fragments arranged by size:
7779 758 443 19
PfoI T'CCnGG_A
Cuts at: 5310 5310
Size: 8999
PmeI GTTT'AAAC
Cuts at: 5998 5998
Size: 8999
PmlI CAC'GTG
Cuts at: 2254 3416 4894 2254
Size: 1162 1478 6359
Fragments arranged by size:
6359 1478 1162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1254 1286 1332 1364 7445 7477 1254
Size: 32 46 32 6081 32 2776
Fragments arranged by size:
6081 2776 46 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 745 3025 5265 5867 5896 745
Size: 2280 2240 602 29 3848
Fragments arranged by size:
3848 2280 2240 602 29
PshAI GACnn'nnGTC
Cuts at: 750 3368 750
Size: 2618 6381
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2772 2804 2772
Size: 32 8967
PstI C_TGCA'G
Cuts at: 6046 6731 6046
Size: 685 8314
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8999
PvuII CAG'CTG
Cuts at: 3231 3377 5417 6424 6784 3231
Size: 146 2040 1007 360 5446
Fragments arranged by size:
5446 2040 1007 360 146
RsrII CG'GwC_CG
Cuts at: 5789 7194 5789
Size: 1405 7594
SacI G_AGCT'C
Cuts at: 6013 6013
Size: 8999
SalI G'TCGA_C
Cuts at: 4439 6036 4439
Size: 1597 7402
SanDI GG'GwC_CC
Cuts at: 5867 5896 5867
Size: 29 8970
SapI GCTCTTCn'nnn_
Cuts at: 7022 7232 7022
Size: 210 8789
SbfI CC_TGCA'GG
Cuts at: 6046 6046
Size: 8999
ScaI AGT'ACT
Cuts at: 711 1035 1296 4848 8516 711
Size: 324 261 3552 3668 1194
Fragments arranged by size:
3668 3552 1194 324 261
SfcI C'TryA_G
Cuts at: 33 371 1256 1298 2537 2990 4643 5574 6042
Size: 338 885 42 1239 453 1653 931 468
Cuts at: 6042 6727 7695 7886 33
Size: 685 968 191 1146
Fragments arranged by size:
1653 1239 1146 968 931 885 685 468
453 338 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 1721 1721
Size: 8999
SfoI GGC'GCC
Cuts at: 6431 6679 6431
Size: 248 8751
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8999
SmaI CCC'GGG
Cuts at: 4141 6021 4141
Size: 1880 7119
SmlI C'TyrA_G
Cuts at: 2152 3578 4199 6154 7069 7783 8045 2152
Size: 1426 621 1955 915 714 262 3106
Fragments arranged by size:
3106 1955 1426 915 714 621 262
SpeI A'CTAG_T
Cuts at: 67 2785 5116 67
Size: 2718 2331 3950
Fragments arranged by size:
3950 2718 2331
SphI G_CATG'C
Cuts at: 4149 5514 6052 6552 4149
Size: 1365 538 500 6596
Fragments arranged by size:
6596 1365 538 500
SspI AAT'ATT
Cuts at: 1284 1443 3849 1284
Size: 159 2406 6434
Fragments arranged by size:
6434 2406 159
StuI AGG'CCT
Cuts at: 490 2362 490
Size: 1872 7127
StyI C'CwwG_G
Cuts at: 591 1023 1042 1056 1206 1356 2646 3917 4041
Size: 432 19 14 150 150 1290 1271 124
Cuts at: 4041 4957 5990 6133 6487 591
Size: 916 1033 143 354 3103
Fragments arranged by size:
3103 1290 1271 1033 916 432 354 150
150 143 124 19 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5806 5962 6582 6715 7174 7383 5806
Size: 156 620 133 459 209 7422
Fragments arranged by size:
7422 620 459 209 156 133
TatI w'GTAC_w
Cuts at: 709 1033 1294 1855 2131 3771 4846 5167 5625
Size: 324 261 561 276 1640 1075 321 458
Cuts at: 5625 8514 709
Size: 2889 1194
Fragments arranged by size:
2889 1640 1194 1075 561 458 324 321
276 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 921 953 1808 1840 2099 2131 5764 5796 8516
Size: 32 855 32 259 32 3633 32 2720
Cuts at: 8516 8548 921
Size: 32 1372
Fragments arranged by size:
3633 2720 1372 855 259 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4573 5214 6796 4573
Size: 641 1582 6776
Fragments arranged by size:
6776 1582 641
XbaI T'CTAG_A
Cuts at: 6030 6030
Size: 8999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 4056 5414 6494 114
Size: 3942 1358 1080 2619
Fragments arranged by size:
3942 2619 1358 1080
XmaI C'CCGG_G
Cuts at: 4139 6019 4139
Size: 1880 7119
XmnI GAAnn'nnTTC
Cuts at: 4561 4561
Size: 8999
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
AvrII BaeI BfrBI BmgBI BsiWI BsmBI BssHII BstEII
BstZ17I EcoRV FseI FspAI MfeI NdeI NotI NruI
NsiI PacI PsiI PspOMI PspXI SacII SexAI SgrAI
SnaBI SrfI SwaI XhoI ZraI