Restriction map for
pF1KA1454
(Circular) (Six-base) MAPSORT of: pF1KA1454.seq Check: 7712 from: 1 to: 7310
pF1KA1454 7310 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3103 3103
Size: 7310
AccI GT'mk_AC
Cuts at: 331 4348 331
Size: 4017 3293
Acc65I G'GTAC_C
Cuts at: 4326 4326
Size: 7310
AclI AA'CG_TT
Cuts at: 7150 7150
Size: 7310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 797 911 1322 1460 1493 1795 2495 2730
Size: 250 114 411 138 33 302 700 235
Cuts at: 2730 2803 3613 5135 5567 5920 6582 547
Size: 73 810 1522 432 353 662 1275
Fragments arranged by size:
1522 1275 810 700 662 432 411 353
302 250 235 138 114 73 33
AfeI AGC'GCT
Cuts at: 124 124
Size: 7310
AflIII A'CryG_T
Cuts at: 4662 6462 6743 4662
Size: 1800 281 5229
Fragments arranged by size:
5229 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 7310
AleI CACnn'nnGTG
Cuts at: 1109 3028 1109
Size: 1919 5391
AlwNI CAG_nnn'CTG
Cuts at: 4615 6053 4615
Size: 1438 5872
ApaLI G'TGCA_C
Cuts at: 6148 6148
Size: 7310
ApoI r'AATT_y
Cuts at: 708 1170 1294 1350 1676 2339 2784 3047 4314
Size: 462 124 56 326 663 445 263 1267
Cuts at: 4314 6663 6919 708
Size: 2349 256 1099
Fragments arranged by size:
2349 1267 1099 663 462 445 326 263
256 124 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 7310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7310
AvaI C'yCGr_G
Cuts at: 2255 4330 2255
Size: 2075 5235
AvrII C'CTAG_G
Cuts at: 2910 2910
Size: 7310
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3160 3193 3160
Size: 33 7277
BamHI G'GATC_C
Cuts at: 3603 4335 3603
Size: 732 6578
BanI G'GyrC_C
Cuts at: 377 1613 3685 4326 4740 4988 5023 377
Size: 1236 2072 641 414 248 35 2664
Fragments arranged by size:
2664 2072 1236 641 414 248 35
BanII G_rGCy'C
Cuts at: 405 2142 4324 5354 405
Size: 1737 2182 1030 2361
Fragments arranged by size:
2361 2182 1737 1030
BbeI G_GCGC'C
Cuts at: 1617 4744 4992 1617
Size: 3127 248 3935
Fragments arranged by size:
3935 3127 248
BbsI GAAGACnn'nnnn_
Cuts at: 586 2094 586
Size: 1508 5802
BbvCI CC'TCA_GC
Cuts at: 4253 4253
Size: 7310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1567 1601 1567
Size: 34 7276
BciVI GTATCCnnnnn_n'
Cuts at: 5208 6264 7310 5208
Size: 1056 1046 5208
Fragments arranged by size:
5208 1056 1046
BclI T'GATC_A
Cuts at: 4043 4830 4043
Size: 787 6523
BfrBI ATG'CAT
Cuts at: 1686 1686
Size: 7310
BglII A'GATC_T
Cuts at: 2835 4825 6862 2835
Size: 1990 2037 3283
Fragments arranged by size:
3283 2037 1990
BlpI GC'TnA_GC
Cuts at: 2411 2416 4422 2411
Size: 5 2006 5299
Fragments arranged by size:
5299 2006 5
Bme1580I G_kGCm'C
Cuts at: 4935 5028 6152 4935
Size: 93 1124 6093
Fragments arranged by size:
6093 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 420 825 1131 3472 4522 4603 4938 7082 420
Size: 405 306 2341 1050 81 335 2144 648
Fragments arranged by size:
2341 2144 1050 648 405 335 306 81
BmtI G_CTAG'C
Cuts at: 1390 1390
Size: 7310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5677 5677
Size: 7310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 1649 2651 3668 4129 4151 359
Size: 1290 1002 1017 461 22 3518
Fragments arranged by size:
3518 1290 1017 1002 461 22
Bpu10I CC'TnA_GC
Cuts at: 1774 1967 2753 4253 1774
Size: 193 786 1500 4831
Fragments arranged by size:
4831 1500 786 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2332 4252 4486 5365 6079 6377 2332
Size: 1920 234 879 714 298 3265
Fragments arranged by size:
3265 1920 879 714 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 895 7014 895
Size: 6119 1191
BsaAI yAC'GTr
Cuts at: 2110 3420 4663 2110
Size: 1310 1243 4757
Fragments arranged by size:
4757 1310 1243
BsaBI GATnn'nnATC
Cuts at: 6 3488 4824 6861 6
Size: 3482 1336 2037 455
Fragments arranged by size:
3482 2037 1336 455
BsaHI Gr'CG_yC
Cuts at: 642 1614 4741 4989 5691 7187 642
Size: 972 3127 248 702 1496 765
Fragments arranged by size:
3127 1496 972 765 702 248
BsaWI w'CCGG_w
Cuts at: 1841 4499 5020 5698 6109 6256 6530 1841
Size: 2658 521 678 411 147 274 2621
Fragments arranged by size:
2658 2621 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 731 178
Size: 553 6757
BseYI C'CCAG_C
Cuts at: 3018 3700 3867 4735 5493 6158 6566 3018
Size: 682 167 868 758 665 408 3762
Fragments arranged by size:
3762 868 758 682 665 408 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1945 2143 3988 874
Size: 1071 198 1845 4196
Fragments arranged by size:
4196 1845 1071 198
BsiEI CG_ry'CG
Cuts at: 104 3978 4852 4898 6128 104
Size: 3874 874 46 1230 1286
Fragments arranged by size:
3874 1286 1230 874 46
BsiHKAI G_wGCw'C
Cuts at: 405 2142 4324 5102 5292 6152 405
Size: 1737 2182 778 190 860 1563
Fragments arranged by size:
2182 1737 1563 860 778 190
BsmI GAATG_Cn'
Cuts at: 3841 3841
Size: 7310
Bsp1286I G_dGCh'C
Cuts at: 405 2142 4324 4935 5028 5102 5292 5354 6152
Size: 1737 2182 611 93 74 190 62 798
Cuts at: 6152 405
Size: 1563
Fragments arranged by size:
2182 1737 1563 798 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1841 1841
Size: 7310
BspHI T'CATG_A
Cuts at: 135 2019 3906 5742 135
Size: 1884 1887 1836 1703
Fragments arranged by size:
1887 1884 1836 1703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1662 3103 4360 4574 4876 5257 1662
Size: 1441 1257 214 302 381 3715
Fragments arranged by size:
3715 1441 1257 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2154 2697 5602 5656 7140 7305 50
Size: 2104 543 2905 54 1484 165 55
Fragments arranged by size:
2905 2104 1484 543 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1421 2502 3075 3545 5222 1421
Size: 1081 573 470 1677 3509
Fragments arranged by size:
3509 1677 1081 573 470
BsrFI r'CCGG_y
Cuts at: 5308 5489 5308
Size: 181 7129
BsrGI T'GTAC_A
Cuts at: 3544 3544
Size: 7310
BssSI C'ACGA_G
Cuts at: 1601 5581 6289 1601
Size: 3980 708 2622
Fragments arranged by size:
3980 2622 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4687 4687
Size: 7310
BstBI TT'CG_AA
Cuts at: 1200 2367 5671 1200
Size: 1167 3304 2839
Fragments arranged by size:
3304 2839 1167
BstXI CCAn_nnnn'nTGG
Cuts at: 3747 3747
Size: 7310
BstYI r'GATC_y
Cuts at: 1 2835 3603 4335 4802 4825 5160 5406 5712
Size: 2834 768 732 467 23 335 246 306
Cuts at: 5712 5724 5810 6862 1
Size: 12 86 1052 449
Fragments arranged by size:
2834 1052 768 732 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1355 1565 3681 4036 107
Size: 1248 210 2116 355 3381
Fragments arranged by size:
3381 2116 1248 355 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5260 5441 5604 5260
Size: 181 163 6966
Fragments arranged by size:
6966 181 163
BtsI GCAGTG_nn'
Cuts at: 314 950 1542 2427 2488 3126 3704 3873 4553
Size: 636 592 885 61 638 578 169 680
Cuts at: 4553 314
Size: 3071
Fragments arranged by size:
3071 885 680 638 636 592 578 169
61
DraI TTT'AAA
Cuts at: 4309 4309
Size: 7310
DraIII CAC_nnn'GTG
Cuts at: 3379 3379
Size: 7310
DrdI GACnn_nn'nnGTC
Cuts at: 5016 6360 5016
Size: 1344 5966
EaeI y'GGCC_r
Cuts at: 3500 3749 3854 3975 4136 4895 5069 5460 5487
Size: 249 105 121 161 759 174 391 27
Cuts at: 5487 6832 3500
Size: 1345 3978
Fragments arranged by size:
3978 1345 759 391 249 174 161 121
105 27
EagI C'GGCC_G
Cuts at: 3975 4895 3975
Size: 920 6390
EarI CTCTTCn'nnn_
Cuts at: 1376 1787 2069 2179 2318 2514 2687 3632 3682
Size: 411 282 110 139 196 173 945 50
Cuts at: 3682 3794 5333 5543 1376
Size: 112 1539 210 3143
Fragments arranged by size:
3143 1539 945 411 282 210 196 173
139 112 110 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5518 6260 6406 7120 5518
Size: 742 146 714 5708
Fragments arranged by size:
5708 742 714 146
EcoICRI GAG'CTC
Cuts at: 403 2140 4322 403
Size: 1737 2182 3391
Fragments arranged by size:
3391 2182 1737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 547 797 911 1322 1460 1493 1649 1795
Size: 188 250 114 411 138 33 156 146
Cuts at: 1795 2495 2651 2730 2803 3613 3668 4129 4151
Size: 700 156 79 73 810 55 461 22
Cuts at: 4151 5135 5567 5920 6582 359
Size: 984 432 353 662 1087
Fragments arranged by size:
1087 984 810 700 662 461 432 411
353 250 188 156 156 146 138 114
79 73 55 33 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3907 3907
Size: 7310
EcoO109I rG'GnC_Cy
Cuts at: 3405 4449 3405
Size: 1044 6266
EcoRI G'AATT_C
Cuts at: 1170 1676 3047 4314 1170
Size: 506 1371 1267 4166
Fragments arranged by size:
4166 1371 1267 506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 3165 4246 5329 2833
Size: 332 1081 1083 4814
Fragments arranged by size:
4814 1083 1081 332
FspI TGC'GCA
Cuts at: 5091 5091
Size: 7310
HaeII r_GCGC'y
Cuts at: 126 1617 4744 4992 6222 6993 126
Size: 1491 3127 248 1230 771 443
Fragments arranged by size:
3127 1491 1230 771 443 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 605 642 674 2014 2046 2854 2886 3288
Size: 32 37 32 1340 32 808 32 402
Cuts at: 3288 3320 3609 3641 3817 3849 4155 4187 5152
Size: 32 289 32 176 32 306 32 965
Cuts at: 5152 5182 5184 5214 573
Size: 30 2 30 2669
Fragments arranged by size:
2669 1340 965 808 402 306 289 176
37 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1454 3179 3942 4349 1454
Size: 1725 763 407 4415
Fragments arranged by size:
4415 1725 763 407
HindIII A'AGCT_T
Cuts at: 702 1807 2675 2815 702
Size: 1105 868 140 5197
Fragments arranged by size:
5197 1105 868 140
HpaI GTT'AAC
Cuts at: 1454 1454
Size: 7310
KasI G'GCGC_C
Cuts at: 1613 4740 4988 1613
Size: 3127 248 3935
Fragments arranged by size:
3935 3127 248
KpnI G_GTAC'C
Cuts at: 4330 4330
Size: 7310
MfeI C'AATT_G
Cuts at: 1721 1937 1721
Size: 216 7094
MluI A'CGCG_T
Cuts at: 6743 6743
Size: 7310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1035 6069 6253 1035
Size: 5034 184 2092
Fragments arranged by size:
5034 2092 184
MscI TGG'CCA
Cuts at: 3502 3751 3856 4138 5071 3502
Size: 249 105 282 933 5741
Fragments arranged by size:
5741 933 282 249 105
MslI CAynn'nnrTG
Cuts at: 612 1109 1685 3028 3745 5299 5426 612
Size: 497 576 1343 717 1554 127 2496
Fragments arranged by size:
2496 1554 1343 717 576 497 127
MspA1I CmG'CkG
Cuts at: 2302 3622 4421 4735 5095 5879 6124 2302
Size: 1320 799 314 360 784 245 3488
Fragments arranged by size:
3488 1320 799 784 360 314 245
NaeI GCC'GGC
Cuts at: 5491 5491
Size: 7310
NarI GG'CG_CC
Cuts at: 1614 4741 4989 1614
Size: 3127 248 3935
Fragments arranged by size:
3935 3127 248
NcoI C'CATG_G
Cuts at: 107 1355 1565 3681 107
Size: 1248 210 2116 3736
Fragments arranged by size:
3736 2116 1248 210
NdeI CA'TA_TG
Cuts at: 3659 3659
Size: 7310
NgoMIV G'CCGG_C
Cuts at: 5489 5489
Size: 7310
NheI G'CTAG_C
Cuts at: 1386 1386
Size: 7310
NsiI A_TGCA'T
Cuts at: 1688 1688
Size: 7310
NspI r_CATG'y
Cuts at: 1686 2434 3520 3538 4363 4863 6466 1686
Size: 748 1086 18 825 500 1603 2530
Fragments arranged by size:
2530 1603 1086 825 748 500 18
PciI A'CATG_T
Cuts at: 6462 6462
Size: 7310
PflMI CCAn_nnn'nTGG
Cuts at: 1571 1672 1571
Size: 101 7209
PfoI T'CCnGG_A
Cuts at: 636 636
Size: 7310
PmeI GTTT'AAAC
Cuts at: 4309 4309
Size: 7310
PmlI CAC'GTG
Cuts at: 2110 2110
Size: 7310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5756 5788 5756
Size: 32 7278
PpuMI rG'GwC_Cy
Cuts at: 3405 3405
Size: 7310
PsiI TTA'TAA
Cuts at: 1990 2807 1990
Size: 817 6493
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1056 1088 1056
Size: 32 7278
PstI C_TGCA'G
Cuts at: 309 3121 3515 3880 3998 4357 5042 309
Size: 2812 394 365 118 359 685 2577
Fragments arranged by size:
2812 2577 685 394 365 359 118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7310
PvuII CAG'CTG
Cuts at: 2302 3622 4735 5095 2302
Size: 1320 1113 360 4517
Fragments arranged by size:
4517 1320 1113 360
RsrII CG'GwC_CG
Cuts at: 5505 5505
Size: 7310
SacI G_AGCT'C
Cuts at: 405 2142 4324 405
Size: 1737 2182 3391
Fragments arranged by size:
3391 2182 1737
SalI G'TCGA_C
Cuts at: 4347 4347
Size: 7310
SapI GCTCTTCn'nnn_
Cuts at: 1376 2069 2687 3632 3794 5333 5543 1376
Size: 693 618 945 162 1539 210 3143
Fragments arranged by size:
3143 1539 945 693 618 210 162
SbfI CC_TGCA'GG
Cuts at: 4357 4357
Size: 7310
ScaI AGT'ACT
Cuts at: 303 4052 6827 303
Size: 3749 2775 786
Fragments arranged by size:
3749 2775 786
SexAI A'CCwGG_T
Cuts at: 3956 3956
Size: 7310
SfcI C'TryA_G
Cuts at: 33 305 1076 2068 3117 3511 3876 3994 4353
Size: 272 771 992 1049 394 365 118 359
Cuts at: 4353 5038 6006 6197 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1049 992 968 771 685 394 365
359 272 191 118
SfoI GGC'GCC
Cuts at: 1615 4742 4990 1615
Size: 3127 248 3935
Fragments arranged by size:
3935 3127 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7310
SmaI CCC'GGG
Cuts at: 2257 4332 2257
Size: 2075 5235
SmlI C'TyrA_G
Cuts at: 2347 4231 4465 5380 6094 6356 2347
Size: 1884 234 915 714 262 3301
Fragments arranged by size:
3301 1884 915 714 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7310
SphI G_CATG'C
Cuts at: 3520 4363 4863 3520
Size: 843 500 5967
Fragments arranged by size:
5967 843 500
StuI AGG'CCT
Cuts at: 862 921 4261 862
Size: 59 3340 3911
Fragments arranged by size:
3911 3340 59
StyI C'CwwG_G
Cuts at: 107 1355 1565 2910 3681 3716 4212 4444 4798
Size: 1248 210 1345 771 35 496 232 354
Cuts at: 4798 107
Size: 2619
Fragments arranged by size:
2619 1345 1248 771 496 354 232 210
35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3962 4893 5026 5485 5694 3962
Size: 931 133 459 209 5578
Fragments arranged by size:
5578 931 459 209 133
TatI w'GTAC_w
Cuts at: 301 1150 2633 2659 2850 3544 4050 6825 301
Size: 849 1483 26 191 694 506 2775 786
Fragments arranged by size:
2775 1483 849 786 694 506 191 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 632 664 3696 3728 6827 6859 632
Size: 32 3032 32 3099 32 1083
Fragments arranged by size:
3099 3032 1083 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5107 5107
Size: 7310
XbaI T'CTAG_A
Cuts at: 1398 2142 4341 1398
Size: 744 2199 4367
Fragments arranged by size:
4367 2199 744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3864 4805 3864
Size: 941 6369
XmaI C'CCGG_G
Cuts at: 2255 4330 2255
Size: 2075 5235
XmnI GAAnn'nnTTC
Cuts at: 1295 2363 1295
Size: 1068 6242
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI ApaI AscI BglI
BmgBI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI NotI NruI PacI
PasI PshAI PspOMI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI