Restriction map for
pF1KA1452
(Circular) (Six-base) MAPSORT of: pF1KA1452.seq Check: 778 from: 1 to: 5237
pF1KA1452 5237 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 737 737
Size: 5237
AccI GT'mk_AC
Cuts at: 2275 2275
Size: 5237
Acc65I G'GTAC_C
Cuts at: 2207 2253 2207
Size: 46 5191
AclI AA'CG_TT
Cuts at: 5077 5077
Size: 5237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1813 1887 3062 3494 3847 4509 1813
Size: 74 1175 432 353 662 2541
Fragments arranged by size:
2541 1175 662 432 353 74
AflIII A'CryG_T
Cuts at: 1523 2589 4389 4670 1523
Size: 1066 1800 281 2090
Fragments arranged by size:
2090 1800 1066 281
AleI CACnn'nnGTG
Cuts at: 1777 1777
Size: 5237
AlwNI CAG_nnn'CTG
Cuts at: 780 1459 1965 2542 3980 780
Size: 679 506 577 1438 2037
Fragments arranged by size:
2037 1438 679 577 506
ApaI G_GGCC'C
Cuts at: 1015 1015
Size: 5237
ApaLI G'TGCA_C
Cuts at: 4075 4075
Size: 5237
ApoI r'AATT_y
Cuts at: 2072 2241 4590 4846 2072
Size: 169 2349 256 2463
Fragments arranged by size:
2463 2349 256 169
AseI AT'TA_AT
Cuts at: 20 20
Size: 5237
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5237
AvaI C'yCGr_G
Cuts at: 1714 1784 2257 1714
Size: 70 473 4694
Fragments arranged by size:
4694 473 70
BamHI G'GATC_C
Cuts at: 1487 2262 1487
Size: 775 4462
BanI G'GyrC_C
Cuts at: 1569 1748 1968 2207 2253 2667 2915 2950 1569
Size: 179 220 239 46 414 248 35 3856
Fragments arranged by size:
3856 414 248 239 220 179 46 35
BanII G_rGCy'C
Cuts at: 479 719 821 1015 1610 1661 2251 3281 479
Size: 240 102 194 595 51 590 1030 2435
Fragments arranged by size:
2435 1030 595 590 240 194 102 51
BbeI G_GCGC'C
Cuts at: 2671 2919 2671
Size: 248 4989
BbsI GAAGACnn'nnnn_
Cuts at: 614 614
Size: 5237
BbvCI CC'TCA_GC
Cuts at: 1145 1145
Size: 5237
BciVI GTATCCnnnnn_n'
Cuts at: 1393 3135 4191 5237 1393
Size: 1742 1056 1046 1393
Fragments arranged by size:
1742 1393 1056 1046
BclI T'GATC_A
Cuts at: 2046 2757 2046
Size: 711 4526
BglI GCCn_nnn'nGGC
Cuts at: 577 1010 1464 1716 577
Size: 433 454 252 4098
Fragments arranged by size:
4098 454 433 252
BglII A'GATC_T
Cuts at: 2142 2752 4789 2142
Size: 610 2037 2590
Fragments arranged by size:
2590 2037 610
BlpI GC'TnA_GC
Cuts at: 1840 2349 1840
Size: 509 4728
Bme1580I G_kGCm'C
Cuts at: 790 1015 1732 1751 2862 2955 4079 790
Size: 225 717 19 1111 93 1124 1948
Fragments arranged by size:
1948 1124 1111 717 225 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 124 827 1089 2449 2530 2865 5009 124
Size: 703 262 1360 81 335 2144 352
Fragments arranged by size:
2144 1360 703 352 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 3604 2161
Size: 1443 3794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1552 2054 1285
Size: 267 502 4468
Fragments arranged by size:
4468 502 267
Bpu10I CC'TnA_GC
Cuts at: 1145 1145
Size: 5237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 780 1834 2111 2413 3292 4006 4304 301
Size: 479 1054 277 302 879 714 298 1234
Fragments arranged by size:
1234 1054 879 714 479 302 298 277
BsaI GGTCTCn'nnnn_
Cuts at: 364 4941 364
Size: 4577 660
BsaAI yAC'GTr
Cuts at: 380 2590 380
Size: 2210 3027
BsaBI GATnn'nnATC
Cuts at: 6 289 955 2051 2751 4788 6
Size: 283 666 1096 700 2037 455
Fragments arranged by size:
2037 1096 700 666 455 283
BsaHI Gr'CG_yC
Cuts at: 545 2668 2916 3618 5114 545
Size: 2123 248 702 1496 668
Fragments arranged by size:
2123 1496 702 668 248
BsaWI w'CCGG_w
Cuts at: 2426 2947 3625 4036 4183 4457 2426
Size: 521 678 411 147 274 3206
Fragments arranged by size:
3206 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 727 886 889 1618 1633 1642 1651 1666 1669
Size: 159 3 729 15 9 9 15 3
Cuts at: 1669 727
Size: 4295
Fragments arranged by size:
4295 729 159 15 15 9 9 3
3
BseYI C'CCAG_C
Cuts at: 517 862 898 920 1199 1298 1511 1675 2662
Size: 345 36 22 279 99 213 164 987
Cuts at: 2662 3420 4085 4493 517
Size: 758 665 408 1261
Fragments arranged by size:
1261 987 758 665 408 345 279 213
164 99 36 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 697 1852 440
Size: 257 1155 3825
Fragments arranged by size:
3825 1155 257
BsiEI CG_ry'CG
Cuts at: 104 646 2779 2825 4055 104
Size: 542 2133 46 1230 1286
Fragments arranged by size:
2133 1286 1230 542 46
BsiHKAI G_wGCw'C
Cuts at: 479 821 1154 1271 1835 2251 3029 3219 4079
Size: 342 333 117 564 416 778 190 860
Cuts at: 4079 479
Size: 1637
Fragments arranged by size:
1637 860 778 564 416 342 333 190
117
Bsp1286I G_dGCh'C
Cuts at: 479 719 790 821 1015 1154 1271 1610 1661
Size: 240 71 31 194 139 117 339 51
Cuts at: 1661 1732 1751 1835 2251 2862 2955 3029 3219
Size: 71 19 84 416 611 93 74 190
Cuts at: 3219 3281 4079 479
Size: 62 798 1637
Fragments arranged by size:
1637 798 611 416 339 240 194 190
139 117 93 84 74 71 71 62
51 31 19
BspHI T'CATG_A
Cuts at: 3669 3669
Size: 5237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 737 788 2287 2501 2803 3184 737
Size: 51 1499 214 302 381 2790
Fragments arranged by size:
2790 1499 381 302 214 51
BsrBI CCG'CTC
Cuts at: 50 1536 3529 3583 5067 5232 50
Size: 1486 1993 54 1484 165 55
Fragments arranged by size:
1993 1486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3149 3149
Size: 5237
BsrFI r'CCGG_y
Cuts at: 1948 3235 3416 1948
Size: 1287 181 3769
Fragments arranged by size:
3769 1287 181
BsrGI T'GTAC_A
Cuts at: 135 135
Size: 5237
BssSI C'ACGA_G
Cuts at: 3508 4216 3508
Size: 708 4529
BstAPI GCAn_nnn'nTGC
Cuts at: 1368 2614 1368
Size: 1246 3991
BstBI TT'CG_AA
Cuts at: 3598 3598
Size: 5237
BstXI CCAn_nnnn'nTGG
Cuts at: 168 168
Size: 5237
BstYI r'GATC_y
Cuts at: 1 956 1487 2142 2262 2729 2752 3087 3333
Size: 955 531 655 120 467 23 335 246
Cuts at: 3333 3639 3651 3737 4789 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 955 655 531 467 449 335 306
246 120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 745 745
Size: 5237
BtgI C'CryG_G
Cuts at: 107 1028 1277 1661 107
Size: 921 249 384 3683
Fragments arranged by size:
3683 921 384 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1075 3187 3368 3531 1075
Size: 2112 181 163 2781
Fragments arranged by size:
2781 2112 181 163
BtsI GCAGTG_nn'
Cuts at: 2480 2480
Size: 5237
ClaI AT'CG_AT
Cuts at: 146 1022 146
Size: 876 4361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 785 820 785
Size: 35 5202
DraI TTT'AAA
Cuts at: 2236 2236
Size: 5237
DraIII CAC_nnn'GTG
Cuts at: 835 835
Size: 5237
DrdI GACnn_nn'nnGTC
Cuts at: 2226 2943 4287 2226
Size: 717 1344 3176
Fragments arranged by size:
3176 1344 717
EaeI y'GGCC_r
Cuts at: 110 158 287 569 643 941 1274 1697 1874
Size: 48 129 282 74 298 333 423 177
Cuts at: 1874 1890 1943 2822 2996 3387 3414 4759 110
Size: 16 53 879 174 391 27 1345 588
Fragments arranged by size:
1345 879 588 423 391 333 298 282
177 174 129 74 53 48 27 16
EagI C'GGCC_G
Cuts at: 643 2822 643
Size: 2179 3058
EarI CTCTTCn'nnn_
Cuts at: 113 423 594 1084 1864 1906 3260 3470 113
Size: 310 171 490 780 42 1354 210 1880
Fragments arranged by size:
1880 1354 780 490 310 210 171 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 619 1566 1663 3445 4187 4333 5047 187
Size: 432 947 97 1782 742 146 714 377
Fragments arranged by size:
1782 947 742 714 432 377 146 97
EcoICRI GAG'CTC
Cuts at: 477 819 2249 477
Size: 342 1430 3465
Fragments arranged by size:
3465 1430 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1552 1813 1887 2054 3062 3494 3847 4509
Size: 267 261 74 167 1008 432 353 662
Cuts at: 4509 1285
Size: 2013
Fragments arranged by size:
2013 1008 662 432 353 267 261 167
74
EcoNI CCTnn'n_nnAGG
Cuts at: 535 535
Size: 5237
EcoO109I rG'GnC_Cy
Cuts at: 356 1012 1456 2376 356
Size: 656 444 920 3217
Fragments arranged by size:
3217 920 656 444
EcoRI G'AATT_C
Cuts at: 2241 2241
Size: 5237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 426 1875 3256 426
Size: 1449 1381 2407
Fragments arranged by size:
2407 1449 1381
FspI TGC'GCA
Cuts at: 928 3018 928
Size: 2090 3147
HaeII r_GCGC'y
Cuts at: 672 1857 2671 2919 4149 4920 672
Size: 1185 814 248 1230 771 989
Fragments arranged by size:
1230 1185 989 814 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 738 742 770 774 1435 1467 2100 2132 3079
Size: 4 28 4 661 32 633 32 947
Cuts at: 3079 3109 3111 3141 738
Size: 30 2 30 2834
Fragments arranged by size:
2834 947 661 633 32 32 30 30
28 4 4 2
HincII GTy'rAC
Cuts at: 2123 2276 2123
Size: 153 5084
KasI G'GCGC_C
Cuts at: 2667 2915 2667
Size: 248 4989
KpnI G_GTAC'C
Cuts at: 2211 2257 2211
Size: 46 5191
MluI A'CGCG_T
Cuts at: 1523 4670 1523
Size: 3147 2090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3996 4180 3996
Size: 184 5053
MscI TGG'CCA
Cuts at: 112 160 289 1699 1876 2998 112
Size: 48 129 1410 177 1122 2351
Fragments arranged by size:
2351 1410 1122 177 129 48
MslI CAynn'nnrTG
Cuts at: 737 1276 1346 1777 3226 3353 737
Size: 539 70 431 1449 127 2621
Fragments arranged by size:
2621 1449 539 431 127 70
MspA1I CmG'CkG
Cuts at: 454 510 924 1133 1199 1359 1485 1548 1563
Size: 56 414 209 66 160 126 63 15
Cuts at: 1563 1679 1907 2348 2662 3022 3806 4051 454
Size: 116 228 441 314 360 784 245 1640
Fragments arranged by size:
1640 784 441 414 360 314 245 228
209 160 126 116 66 63 56 15
NaeI GCC'GGC
Cuts at: 3418 3418
Size: 5237
NarI GG'CG_CC
Cuts at: 2668 2916 2668
Size: 248 4989
NcoI C'CATG_G
Cuts at: 107 1661 107
Size: 1554 3683
NgoMIV G'CCGG_C
Cuts at: 3416 3416
Size: 5237
NspI r_CATG'y
Cuts at: 1371 1926 2205 2290 2790 4393 1371
Size: 555 279 85 500 1603 2215
Fragments arranged by size:
2215 1603 555 500 279 85
PasI CC'CwG_GG
Cuts at: 719 1758 719
Size: 1039 4198
PciI A'CATG_T
Cuts at: 4389 4389
Size: 5237
PflMI CCAn_nnn'nTGG
Cuts at: 1056 1056
Size: 5237
PfoI T'CCnGG_A
Cuts at: 1262 1262
Size: 5237
PmeI GTTT'AAAC
Cuts at: 2236 2236
Size: 5237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 5205
PspOMI G'GGCC_C
Cuts at: 1011 1011
Size: 5237
PstI C_TGCA'G
Cuts at: 509 698 1159 1547 2284 2969 509
Size: 189 461 388 737 685 2777
Fragments arranged by size:
2777 737 685 461 388 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5237
PvuII CAG'CTG
Cuts at: 510 924 1563 2662 3022 510
Size: 414 639 1099 360 2725
Fragments arranged by size:
2725 1099 639 414 360
RsrII CG'GwC_CG
Cuts at: 1917 3432 1917
Size: 1515 3722
SacI G_AGCT'C
Cuts at: 479 821 2251 479
Size: 342 1430 3465
Fragments arranged by size:
3465 1430 342
SalI G'TCGA_C
Cuts at: 2274 2274
Size: 5237
SapI GCTCTTCn'nnn_
Cuts at: 423 3260 3470 423
Size: 2837 210 2190
Fragments arranged by size:
2837 2190 210
SbfI CC_TGCA'GG
Cuts at: 2284 2284
Size: 5237
ScaI AGT'ACT
Cuts at: 2171 4754 2171
Size: 2583 2654
SfcI C'TryA_G
Cuts at: 33 462 505 694 1155 1543 1927 2280 2965
Size: 429 43 189 461 388 384 353 685
Cuts at: 2965 3933 4124 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 461 429 388 384 353
191 189 43
SfoI GGC'GCC
Cuts at: 2669 2917 2669
Size: 248 4989
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5237
SmaI CCC'GGG
Cuts at: 2259 2259
Size: 5237
SmlI C'TyrA_G
Cuts at: 280 795 1849 2126 2392 3307 4021 4283 280
Size: 515 1054 277 266 915 714 262 1234
Fragments arranged by size:
1234 1054 915 714 515 277 266 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5237
SphI G_CATG'C
Cuts at: 1926 2290 2790 1926
Size: 364 500 4373
Fragments arranged by size:
4373 500 364
StuI AGG'CCT
Cuts at: 1798 1798
Size: 5237
StyI C'CwwG_G
Cuts at: 107 1071 1109 1505 1661 2371 2725 107
Size: 964 38 396 156 710 354 2619
Fragments arranged by size:
2619 964 710 396 354 156 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 846 876 1071 1191 2820 2953 3412 3621 846
Size: 30 195 120 1629 133 459 209 2462
Fragments arranged by size:
2462 1629 459 209 195 133 120 30
TatI w'GTAC_w
Cuts at: 135 152 2169 2223 4752 135
Size: 17 2017 54 2529 620
Fragments arranged by size:
2529 2017 620 54 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4754 4786 4754
Size: 32 5205
Tth111I GACn'n_nGTC
Cuts at: 3034 3034
Size: 5237
XbaI T'CTAG_A
Cuts at: 2268 2268
Size: 5237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1026 1776 2732 1026
Size: 750 956 3531
Fragments arranged by size:
3531 956 750
XmaI C'CCGG_G
Cuts at: 2257 2257
Size: 5237
XmnI GAAnn'nnTTC
Cuts at: 1258 1798 1258
Size: 540 4697
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BspEI BssHII BstEII BstZ17I EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI