Restriction map for
pF1KA1450
(Circular) (Six-base) MAPSORT of: pF1KA1450.seq Check: 3773 from: 1 to: 6455
pF1KA1450 6455 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 3493 3493
Size: 6455
Acc65I G'GTAC_C
Cuts at: 3471 3471
Size: 6455
AclI AA'CG_TT
Cuts at: 6295 6295
Size: 6455
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1168 2733 2777 2810 3037 3104 3196 4280
Size: 155 1565 44 33 227 67 92 1084
Cuts at: 4280 4712 5459 5727 1013
Size: 432 747 268 1741
Fragments arranged by size:
1741 1565 1084 747 432 268 227 155
92 67 44 33
AfeI AGC'GCT
Cuts at: 1354 1354
Size: 6455
AflIII A'CryG_T
Cuts at: 515 3807 4911 5888 515
Size: 3292 1104 977 1082
Fragments arranged by size:
3292 1104 1082 977
AgeI A'CCGG_T
Cuts at: 2195 4843 2195
Size: 2648 3807
AleI CACnn'nnGTG
Cuts at: 1621 2852 1621
Size: 1231 5224
AlwNI CAG_nnn'CTG
Cuts at: 644 2293 3027 3760 5327 644
Size: 1649 734 733 1567 1772
Fragments arranged by size:
1772 1649 1567 734 733
ApaI G_GGCC'C
Cuts at: 2692 2692
Size: 6455
ApaLI G'TGCA_C
Cuts at: 5225 5225
Size: 6455
ApoI r'AATT_y
Cuts at: 1089 1249 3459 5808 6064 1089
Size: 160 2210 2349 256 1480
Fragments arranged by size:
2349 2210 1480 256 160
AseI AT'TA_AT
Cuts at: 20 20
Size: 6455
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6455
AvaI C'yCGr_G
Cuts at: 3475 3475
Size: 6455
BamHI G'GATC_C
Cuts at: 3480 3480
Size: 6455
BanI G'GyrC_C
Cuts at: 2003 2076 3471 3885 4133 4168 2003
Size: 73 1395 414 248 35 4290
Fragments arranged by size:
4290 1395 414 248 73 35
BanII G_rGCy'C
Cuts at: 192 1835 2407 2692 3469 4499 192
Size: 1643 572 285 777 1030 2148
Fragments arranged by size:
2148 1643 1030 777 572 285
BbeI G_GCGC'C
Cuts at: 3889 4137 3889
Size: 248 6207
BbsI GAAGACnn'nnnn_
Cuts at: 612 812 612
Size: 200 6255
BciVI GTATCCnnnnn_n'
Cuts at: 4353 5114 6455 4353
Size: 761 1341 4353
Fragments arranged by size:
4353 1341 761
BclI T'GATC_A
Cuts at: 854 3975 854
Size: 3121 3334
BfrBI ATG'CAT
Cuts at: 2047 3267 2047
Size: 1220 5235
BglI GCCn_nnn'nGGC
Cuts at: 1916 2365 1916
Size: 449 6006
BglII A'GATC_T
Cuts at: 2838 3970 6007 2838
Size: 1132 2037 3286
Fragments arranged by size:
3286 2037 1132
BlpI GC'TnA_GC
Cuts at: 2545 3567 2545
Size: 1022 5433
Bme1580I G_kGCm'C
Cuts at: 1566 1912 1921 2569 2692 4080 4173 5229 1566
Size: 346 9 648 123 1388 93 1056 2792
Fragments arranged by size:
2792 1388 1056 648 346 123 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 1509 3379 3667 3748 4083 6227 1509
Size: 1870 288 81 335 2144 1737
Fragments arranged by size:
2144 1870 1737 335 288 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1628 4822 1628
Size: 3194 3261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 1583 1737 2270 2629 2677 2885 109
Size: 1474 154 533 359 48 208 3679
Fragments arranged by size:
3679 1474 533 359 208 154 48
Bpu10I CC'TnA_GC
Cuts at: 464 2381 464
Size: 1917 4538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 3631 4510 5002 5300 1345
Size: 2286 879 492 298 2500
Fragments arranged by size:
2500 2286 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1925 2906 3192 6159 1925
Size: 981 286 2967 2221
Fragments arranged by size:
2967 2221 981 286
BsaAI yAC'GTr
Cuts at: 3808 3808
Size: 6455
BsaBI GATnn'nnATC
Cuts at: 6 3969 6006 6
Size: 3963 2037 455
Fragments arranged by size:
3963 2037 455
BsaHI Gr'CG_yC
Cuts at: 172 3886 4134 4836 6332 172
Size: 3714 248 702 1496 295
Fragments arranged by size:
3714 1496 702 295 248
BsaWI w'CCGG_w
Cuts at: 2195 3644 4165 4843 5117 5264 5675 2195
Size: 1449 521 678 274 147 411 2975
Fragments arranged by size:
2975 1449 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 1789 1819 3408 3438 547
Size: 30 1212 30 1589 30 3564
Fragments arranged by size:
3564 1589 1212 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 584 870 1810 2616 340
Size: 244 286 940 806 4179
Fragments arranged by size:
4179 940 806 286 244
BseYI C'CCAG_C
Cuts at: 1569 2104 2536 3880 4638 5215 5711 1569
Size: 535 432 1344 758 577 496 2313
Fragments arranged by size:
2313 1344 758 577 535 496 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 3145 2603
Size: 542 5913
BsiEI CG_ry'CG
Cuts at: 104 3997 4043 5251 104
Size: 3893 46 1208 1308
Fragments arranged by size:
3893 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3469 4247 4437 5229 3469
Size: 778 190 792 4695
Fragments arranged by size:
4695 792 778 190
BsmI GAATG_Cn'
Cuts at: 2049 2049
Size: 6455
Bsp1286I G_dGCh'C
Cuts at: 192 1566 1835 1912 1921 2407 2569 2692 3469
Size: 1374 269 77 9 486 162 123 777
Cuts at: 3469 4080 4173 4247 4437 4499 5229 192
Size: 611 93 74 190 62 730 1418
Fragments arranged by size:
1418 1374 777 730 611 486 269 190
162 123 93 77 74 62 9
BspEI T'CCGG_A
Cuts at: 5675 5675
Size: 6455
BspHI T'CATG_A
Cuts at: 5631 5631
Size: 6455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2382 3117 3398 3505 3719 4021 4402 2382
Size: 735 281 107 214 302 381 4435
Fragments arranged by size:
4435 735 381 302 281 214 107
BsrBI CCG'CTC
Cuts at: 50 1837 2942 4747 4801 6285 6450 50
Size: 1787 1105 1805 54 1484 165 55
Fragments arranged by size:
1805 1787 1484 1105 165 55 54
BsrDI GCAATG_nn'
Cuts at: 821 4367 821
Size: 3546 2909
BsrFI r'CCGG_y
Cuts at: 2195 2998 4453 4634 4843 2195
Size: 803 1455 181 209 3807
Fragments arranged by size:
3807 1455 803 209 181
BssSI C'ACGA_G
Cuts at: 2698 3338 4726 5084 2698
Size: 640 1388 358 4069
Fragments arranged by size:
4069 1388 640 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2533 3832 2533
Size: 1299 5156
BstBI TT'CG_AA
Cuts at: 921 4816 921
Size: 3895 2560
BstEII G'GTnAC_C
Cuts at: 1738 1738
Size: 6455
BstYI r'GATC_y
Cuts at: 1 448 693 2838 3480 3947 3970 4305 4551
Size: 447 245 2145 642 467 23 335 246
Cuts at: 4551 5563 5649 5661 6007 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2145 1012 642 467 449 447 346 335
246 245 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 194 194
Size: 6455
BtgI C'CryG_G
Cuts at: 107 164 2577 107
Size: 57 2413 3985
Fragments arranged by size:
3985 2413 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4405 4586 4749 4405
Size: 181 163 6111
Fragments arranged by size:
6111 181 163
BtsI GCAGTG_nn'
Cuts at: 402 3009 3028 3698 402
Size: 2607 19 670 3159
Fragments arranged by size:
3159 2607 670 19
DraI TTT'AAA
Cuts at: 1751 2412 3454 1751
Size: 661 1042 4752
Fragments arranged by size:
4752 1042 661
DrdI GACnn_nn'nnGTC
Cuts at: 2868 4161 5019 2868
Size: 1293 858 4304
Fragments arranged by size:
4304 1293 858
EaeI y'GGCC_r
Cuts at: 1532 1888 2937 3032 4040 4214 4605 4632 5977
Size: 356 1049 95 1008 174 391 27 1345
Cuts at: 5977 1532
Size: 2010
Fragments arranged by size:
2010 1345 1049 1008 391 356 174 95
27
EagI C'GGCC_G
Cuts at: 4040 4040
Size: 6455
EarI CTCTTCn'nnn_
Cuts at: 139 432 1144 2097 4478 4688 139
Size: 293 712 953 2381 210 1906
Fragments arranged by size:
2381 1906 953 712 293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4663 4973 5119 6265 4663
Size: 310 146 1146 4853
Fragments arranged by size:
4853 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3467 3467
Size: 6455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 1013 1168 1583 1737 2270 2629 2677 2733
Size: 904 155 415 154 533 359 48 56
Cuts at: 2733 2777 2810 2885 3037 3104 3196 4280 4712
Size: 44 33 75 152 67 92 1084 432
Cuts at: 4712 5459 5727 109
Size: 747 268 837
Fragments arranged by size:
1084 904 837 747 533 432 415 359
268 155 154 152 92 75 67 56
48 44 33
EcoNI CCTnn'n_nnAGG
Cuts at: 197 197
Size: 6455
EcoO109I rG'GnC_Cy
Cuts at: 202 736 1452 2385 2647 2688 2689 2870 3594
Size: 534 716 933 262 41 1 181 724
Cuts at: 3594 202
Size: 3063
Fragments arranged by size:
3063 933 724 716 534 262 181 41
1
EcoRI G'AATT_C
Cuts at: 1249 3459 1249
Size: 2210 4245
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 518 2763 4474 518
Size: 2245 1711 2499
Fragments arranged by size:
2499 2245 1711
FspI TGC'GCA
Cuts at: 4236 4236
Size: 6455
HaeII r_GCGC'y
Cuts at: 912 1356 2795 3889 4137 5159 6138 912
Size: 444 1439 1094 248 1022 979 1229
Fragments arranged by size:
1439 1229 1094 1022 979 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1224 1247 1256 1279 1710 1742 4297 4327 4329
Size: 23 9 23 431 32 2555 30 2
Cuts at: 4329 4359 1224
Size: 30 3320
Fragments arranged by size:
3320 2555 431 32 30 30 23 23
9 2
HincII GTy'rAC
Cuts at: 513 968 1433 3401 3494 513
Size: 455 465 1968 93 3474
Fragments arranged by size:
3474 1968 465 455 93
HindIII A'AGCT_T
Cuts at: 2274 3235 2274
Size: 961 5494
HpaI GTT'AAC
Cuts at: 1433 1433
Size: 6455
KasI G'GCGC_C
Cuts at: 3885 4133 3885
Size: 248 6207
KpnI G_GTAC'C
Cuts at: 3475 3475
Size: 6455
MfeI C'AATT_G
Cuts at: 1157 1157
Size: 6455
MluI A'CGCG_T
Cuts at: 5888 5888
Size: 6455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3407 5126 5310 3407
Size: 1719 184 4552
Fragments arranged by size:
4552 1719 184
MscI TGG'CCA
Cuts at: 1534 4216 1534
Size: 2682 3773
MslI CAynn'nnrTG
Cuts at: 1468 1621 1819 2852 2971 4444 4571 1468
Size: 153 198 1033 119 1473 127 3352
Fragments arranged by size:
3352 1473 1033 198 153 127 119
MspA1I CmG'CkG
Cuts at: 155 166 914 1569 1710 2108 2685 3566 3880
Size: 11 748 655 141 398 577 881 314
Cuts at: 3880 4240 5253 5498 155
Size: 360 1013 245 1112
Fragments arranged by size:
1112 1013 881 748 655 577 398 360
314 245 141 11
NaeI GCC'GGC
Cuts at: 4636 4636
Size: 6455
NarI GG'CG_CC
Cuts at: 3886 4134 3886
Size: 248 6207
NcoI C'CATG_G
Cuts at: 107 107
Size: 6455
NgoMIV G'CCGG_C
Cuts at: 4634 4634
Size: 6455
NsiI A_TGCA'T
Cuts at: 2049 3269 2049
Size: 1220 5235
NspI r_CATG'y
Cuts at: 519 1530 2976 3508 4008 4915 519
Size: 1011 1446 532 500 907 2059
Fragments arranged by size:
2059 1446 1011 907 532 500
PasI CC'CwG_GG
Cuts at: 180 2796 180
Size: 2616 3839
PciI A'CATG_T
Cuts at: 515 4911 515
Size: 4396 2059
PfoI T'CCnGG_A
Cuts at: 1094 2029 2231 3065 1094
Size: 935 202 834 4484
Fragments arranged by size:
4484 935 834 202
PmeI GTTT'AAAC
Cuts at: 3454 3454
Size: 6455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5594 5626 5594
Size: 32 6423
PpuMI rG'GwC_Cy
Cuts at: 202 736 1452 2385 2647 2870 202
Size: 534 716 933 262 223 3787
Fragments arranged by size:
3787 933 716 534 262 223
PshAI GACnn'nnGTC
Cuts at: 1937 1937
Size: 6455
PspOMI G'GGCC_C
Cuts at: 2688 2688
Size: 6455
PstI C_TGCA'G
Cuts at: 3502 4187 3502
Size: 685 5770
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6455
PvuII CAG'CTG
Cuts at: 1569 1710 2108 3880 4240 1569
Size: 141 398 1772 360 3784
Fragments arranged by size:
3784 1772 398 360 141
RsrII CG'GwC_CG
Cuts at: 4650 4650
Size: 6455
SacI G_AGCT'C
Cuts at: 3469 3469
Size: 6455
SacII CC_GC'GG
Cuts at: 167 167
Size: 6455
SalI G'TCGA_C
Cuts at: 3492 3492
Size: 6455
SanDI GG'GwC_CC
Cuts at: 1452 1452
Size: 6455
SapI GCTCTTCn'nnn_
Cuts at: 139 2097 4478 4688 139
Size: 1958 2381 210 1906
Fragments arranged by size:
2381 1958 1906 210
SbfI CC_TGCA'GG
Cuts at: 3502 3502
Size: 6455
ScaI AGT'ACT
Cuts at: 3417 5972 3417
Size: 2555 3900
SfcI C'TryA_G
Cuts at: 33 1002 1179 3283 3498 4183 5176 5367 33
Size: 969 177 2104 215 685 993 191 1121
Fragments arranged by size:
2104 1121 993 969 685 215 191 177
SfoI GGC'GCC
Cuts at: 3887 4135 3887
Size: 248 6207
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6455
SmaI CCC'GGG
Cuts at: 3477 3477
Size: 6455
SmlI C'TyrA_G
Cuts at: 1360 3610 4525 5017 5279 1360
Size: 2250 915 492 262 2536
Fragments arranged by size:
2536 2250 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6455
SphI G_CATG'C
Cuts at: 3508 4008 3508
Size: 500 5955
StuI AGG'CCT
Cuts at: 2338 2338
Size: 6455
StyI C'CwwG_G
Cuts at: 107 1285 1775 2351 2873 2896 3589 3943 107
Size: 1178 490 576 522 23 693 354 2619
Fragments arranged by size:
2619 1178 693 576 522 490 354 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1561 2932 4038 4171 4630 4839 1561
Size: 1371 1106 133 459 209 3177
Fragments arranged by size:
3177 1371 1106 459 209 133
TatI w'GTAC_w
Cuts at: 488 652 680 3415 5970 488
Size: 164 28 2735 2555 973
Fragments arranged by size:
2735 2555 973 164 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2988 3020 5972 6004 2988
Size: 32 2952 32 3439
Fragments arranged by size:
3439 2952 32 32
Tth111I GACn'n_nGTC
Cuts at: 1645 3040 4252 1645
Size: 1395 1212 3848
Fragments arranged by size:
3848 1395 1212
XbaI T'CTAG_A
Cuts at: 3486 3486
Size: 6455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 439 1750 2689 2838 3950 439
Size: 1311 939 149 1112 2944
Fragments arranged by size:
2944 1311 1112 939 149
XmaI C'CCGG_G
Cuts at: 3475 3475
Size: 6455
XmnI GAAnn'nnTTC
Cuts at: 133 473 133
Size: 340 6115
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI NdeI NheI NotI NruI PacI PflMI PmlI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI