Restriction map for
pF1KA1434
(Circular) (Six-base) MAPSORT of: pF1KA1434.seq Check: 152 from: 1 to: 5129
pF1KA1434 5129 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2167 2167
Size: 5129
Acc65I G'GTAC_C
Cuts at: 2145 2145
Size: 5129
AclI AA'CG_TT
Cuts at: 4969 4969
Size: 5129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2017 2954 3386 4133 4401 1814
Size: 203 937 432 747 268 2542
Fragments arranged by size:
2542 937 747 432 268 203
AflII C'TTAA_G
Cuts at: 1429 2017 1429
Size: 588 4541
AflIII A'CryG_T
Cuts at: 490 858 2481 3585 4562 490
Size: 368 1623 1104 977 1057
Fragments arranged by size:
1623 1104 1057 977 368
AgeI A'CCGG_T
Cuts at: 3517 3517
Size: 5129
AleI CACnn'nnGTG
Cuts at: 458 1342 458
Size: 884 4245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1647 1647
Size: 5129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 281 313 281
Size: 32 5097
AlwNI CAG_nnn'CTG
Cuts at: 2434 4001 2434
Size: 1567 3562
ApaLI G'TGCA_C
Cuts at: 3899 3899
Size: 5129
ApoI r'AATT_y
Cuts at: 917 945 1180 1258 1289 1395 1584 2133 4482
Size: 28 235 78 31 106 189 549 2349
Cuts at: 4482 4738 917
Size: 256 1308
Fragments arranged by size:
2349 1308 549 256 235 189 106 78
31 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 5129
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
AvaI C'yCGr_G
Cuts at: 2149 2149
Size: 5129
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 999 1032 999
Size: 33 5096
BamHI G'GATC_C
Cuts at: 2154 2154
Size: 5129
BanI G'GyrC_C
Cuts at: 2145 2559 2807 2842 2145
Size: 414 248 35 4432
Fragments arranged by size:
4432 414 248 35
BanII G_rGCy'C
Cuts at: 2143 3173 2143
Size: 1030 4099
BbeI G_GCGC'C
Cuts at: 2563 2811 2563
Size: 248 4881
BbsI GAAGACnn'nnnn_
Cuts at: 1802 1802
Size: 5129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 724 758 724
Size: 34 5095
BciVI GTATCCnnnnn_n'
Cuts at: 1000 3027 3788 5129 1000
Size: 2027 761 1341 1000
Fragments arranged by size:
2027 1341 1000 761
BclI T'GATC_A
Cuts at: 2649 2649
Size: 5129
BglI GCCn_nnn'nGGC
Cuts at: 1111 1111
Size: 5129
BglII A'GATC_T
Cuts at: 816 1723 2644 4681 816
Size: 907 921 2037 1264
Fragments arranged by size:
2037 1264 921 907
BlpI GC'TnA_GC
Cuts at: 2241 2241
Size: 5129
Bme1580I G_kGCm'C
Cuts at: 1214 2754 2847 3903 1214
Size: 1540 93 1056 2440
Fragments arranged by size:
2440 1540 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1783 2341 2422 2757 4901 1783
Size: 558 81 335 2144 2011
Fragments arranged by size:
2144 2011 558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1306 625
Size: 681 4448
Bpu10I CC'TnA_GC
Cuts at: 1838 1838
Size: 5129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2305 3184 3676 3974 1667
Size: 638 879 492 298 2822
Fragments arranged by size:
2822 879 638 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4833 4833
Size: 5129
BsaAI yAC'GTr
Cuts at: 831 2482 831
Size: 1651 3478
BsaBI GATnn'nnATC
Cuts at: 6 1268 2643 4680 6
Size: 1262 1375 2037 455
Fragments arranged by size:
2037 1375 1262 455
BsaHI Gr'CG_yC
Cuts at: 2560 2808 3510 5006 2560
Size: 248 702 1496 2683
Fragments arranged by size:
2683 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2318 2839 3517 3791 3938 4349 2318
Size: 521 678 274 147 411 3098
Fragments arranged by size:
3098 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 281 311 281
Size: 30 5099
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 302 1611 302
Size: 1309 3820
BseYI C'CCAG_C
Cuts at: 1106 1859 2554 3312 3889 4385 1106
Size: 753 695 758 577 496 1850
Fragments arranged by size:
1850 758 753 695 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 1103 1187 1334 720
Size: 383 84 147 4515
Fragments arranged by size:
4515 383 147 84
BsiEI CG_ry'CG
Cuts at: 104 2671 2717 3925 104
Size: 2567 46 1208 1308
Fragments arranged by size:
2567 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 830 2143 2921 3111 3903 830
Size: 1313 778 190 792 2056
Fragments arranged by size:
2056 1313 792 778 190
BsmI GAATG_Cn'
Cuts at: 705 2124 705
Size: 1419 3710
Bsp1286I G_dGCh'C
Cuts at: 830 1214 2143 2754 2847 2921 3111 3173 3903
Size: 384 929 611 93 74 190 62 730
Cuts at: 3903 830
Size: 2056
Fragments arranged by size:
2056 929 730 611 384 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4349 4349
Size: 5129
BspHI T'CATG_A
Cuts at: 935 1224 4305 935
Size: 289 3081 1759
Fragments arranged by size:
3081 1759 289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2179 2393 2695 3076 2179
Size: 214 302 381 4232
Fragments arranged by size:
4232 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3421 3475 4959 5124 50
Size: 3371 54 1484 165 55
Fragments arranged by size:
3371 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 890 1739 1751 3041 890
Size: 849 12 1290 2978
Fragments arranged by size:
2978 1290 849 12
BsrFI r'CCGG_y
Cuts at: 3127 3308 3517 3127
Size: 181 209 4739
Fragments arranged by size:
4739 209 181
BsrGI T'GTAC_A
Cuts at: 1785 1785
Size: 5129
BssSI C'ACGA_G
Cuts at: 3400 3758 3400
Size: 358 4771
BstAPI GCAn_nnn'nTGC
Cuts at: 2007 2506 2007
Size: 499 4630
BstBI TT'CG_AA
Cuts at: 809 3490 809
Size: 2681 2448
BstXI CCAn_nnnn'nTGG
Cuts at: 663 663
Size: 5129
BstYI r'GATC_y
Cuts at: 1 816 1487 1723 2154 2621 2644 2979 3225
Size: 815 671 236 431 467 23 335 246
Cuts at: 3225 4237 4323 4335 4681 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 815 671 467 449 431 346 335
246 236 86 23 12
BtgI C'CryG_G
Cuts at: 1214 1214
Size: 5129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3079 3260 3423 3079
Size: 181 163 4785
Fragments arranged by size:
4785 181 163
BtsI GCAGTG_nn'
Cuts at: 2372 2372
Size: 5129
DraI TTT'AAA
Cuts at: 1568 2128 1568
Size: 560 4569
DrdI GACnn_nn'nnGTC
Cuts at: 2835 3693 2835
Size: 858 4271
EaeI y'GGCC_r
Cuts at: 1071 2714 2888 3279 3306 4651 1071
Size: 1643 174 391 27 1345 1549
Fragments arranged by size:
1643 1549 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2714 2714
Size: 5129
EarI CTCTTCn'nnn_
Cuts at: 230 932 1588 3152 3362 230
Size: 702 656 1564 210 1997
Fragments arranged by size:
1997 1564 702 656 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3337 3647 3793 4939 3337
Size: 310 146 1146 3527
Fragments arranged by size:
3527 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2141 2141
Size: 5129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1306 1814 2017 2954 3386 4133 4401 625
Size: 681 508 203 937 432 747 268 1353
Fragments arranged by size:
1353 937 747 681 508 432 268 203
EcoNI CCTnn'n_nnAGG
Cuts at: 1198 1999 1198
Size: 801 4328
EcoO109I rG'GnC_Cy
Cuts at: 2268 2268
Size: 5129
EcoRI G'AATT_C
Cuts at: 917 2133 917
Size: 1216 3913
EcoRV GAT'ATC
Cuts at: 671 2082 671
Size: 1411 3718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 5129
FspI TGC'GCA
Cuts at: 2910 2910
Size: 5129
HaeII r_GCGC'y
Cuts at: 2563 2811 3833 4812 2563
Size: 248 1022 979 2880
Fragments arranged by size:
2880 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 613 645 1009 1041 1708 1740 2971
Size: 32 479 32 364 32 667 32 1231
Cuts at: 2971 3001 3003 3033 102
Size: 30 2 30 2198
Fragments arranged by size:
2198 1231 667 479 364 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 978 2168 978
Size: 1190 3939
KasI G'GCGC_C
Cuts at: 2559 2807 2559
Size: 248 4881
KpnI G_GTAC'C
Cuts at: 2149 2149
Size: 5129
MfeI C'AATT_G
Cuts at: 1984 1984
Size: 5129
MluI A'CGCG_T
Cuts at: 4562 4562
Size: 5129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 727 3800 3984 727
Size: 3073 184 1872
Fragments arranged by size:
3073 1872 184
MscI TGG'CCA
Cuts at: 1073 2890 1073
Size: 1817 3312
MslI CAynn'nnrTG
Cuts at: 458 1079 1342 1744 2064 2090 3118 3245 458
Size: 621 263 402 320 26 1028 127 2342
Fragments arranged by size:
2342 1028 621 402 320 263 127 26
MspA1I CmG'CkG
Cuts at: 1110 2240 2554 2914 3927 4172 1110
Size: 1130 314 360 1013 245 2067
Fragments arranged by size:
2067 1130 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 5129
NarI GG'CG_CC
Cuts at: 2560 2808 2560
Size: 248 4881
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 5129
NspI r_CATG'y
Cuts at: 258 494 862 2182 2682 3589 258
Size: 236 368 1320 500 907 1798
Fragments arranged by size:
1798 1320 907 500 368 236
PciI A'CATG_T
Cuts at: 490 858 3585 490
Size: 368 2727 2034
Fragments arranged by size:
2727 2034 368
PflMI CCAn_nnn'nTGG
Cuts at: 1043 1043
Size: 5129
PfoI T'CCnGG_A
Cuts at: 852 949 852
Size: 97 5032
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 281 313 4268 4300 281
Size: 32 3955 32 1110
Fragments arranged by size:
3955 1110 32 32
PstI C_TGCA'G
Cuts at: 2176 2861 2176
Size: 685 4444
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5129
PvuII CAG'CTG
Cuts at: 1110 2554 2914 1110
Size: 1444 360 3325
Fragments arranged by size:
3325 1444 360
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5129
SacI G_AGCT'C
Cuts at: 2143 2143
Size: 5129
SalI G'TCGA_C
Cuts at: 2166 2166
Size: 5129
SapI GCTCTTCn'nnn_
Cuts at: 230 3152 3362 230
Size: 2922 210 1997
Fragments arranged by size:
2922 1997 210
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5129
ScaI AGT'ACT
Cuts at: 4646 4646
Size: 5129
SfcI C'TryA_G
Cuts at: 33 2172 2857 3850 4041 33
Size: 2139 685 993 191 1121
Fragments arranged by size:
2139 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2561 2809 2561
Size: 248 4881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
SmaI CCC'GGG
Cuts at: 2151 2151
Size: 5129
SmlI C'TyrA_G
Cuts at: 1429 1682 2017 2284 3199 3691 3953 1429
Size: 253 335 267 915 492 262 2605
Fragments arranged by size:
2605 915 492 335 267 262 253
SpeI A'CTAG_T
Cuts at: 67 1845 67
Size: 1778 3351
SphI G_CATG'C
Cuts at: 258 2182 2682 258
Size: 1924 500 2705
Fragments arranged by size:
2705 1924 500
StuI AGG'CCT
Cuts at: 602 602
Size: 5129
StyI C'CwwG_G
Cuts at: 2263 2617 2263
Size: 354 4775
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2712 2845 3304 3513 2712
Size: 133 459 209 4328
Fragments arranged by size:
4328 459 209 133
TatI w'GTAC_w
Cuts at: 276 639 755 1785 4644 276
Size: 363 116 1030 2859 761
Fragments arranged by size:
2859 1030 761 363 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 637 669 1495 1527 4646 4678 637
Size: 32 826 32 3119 32 1088
Fragments arranged by size:
3119 1088 826 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5129
XbaI T'CTAG_A
Cuts at: 571 2160 571
Size: 1589 3540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1501 2624 1501
Size: 1123 4006
XmaI C'CCGG_G
Cuts at: 2149 2149
Size: 5129
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI ApaI AscI AvrII BbvCI
BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI