Restriction map for
pF1KA1430
(Circular) (Six-base) MAPSORT of: pF1KA1430.seq Check: 880 from: 1 to: 4709
pF1KA1430 4709 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1747 1747
Size: 4709
Acc65I G'GTAC_C
Cuts at: 1725 1725
Size: 4709
AclI AA'CG_TT
Cuts at: 578 1476 4549 578
Size: 898 3073 738
Fragments arranged by size:
3073 898 738
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 872 889 1064 1199 2534 2966 3713 3981
Size: 237 17 175 135 1335 432 747 268
Cuts at: 3981 635
Size: 1363
Fragments arranged by size:
1363 1335 747 432 268 237 175 135
17
AflIII A'CryG_T
Cuts at: 444 446 735 2061 3165 4142 444
Size: 2 289 1326 1104 977 1011
Fragments arranged by size:
1326 1104 1011 977 289 2
AgeI A'CCGG_T
Cuts at: 3097 3097
Size: 4709
AlwNI CAG_nnn'CTG
Cuts at: 685 2014 3581 685
Size: 1329 1567 1813
Fragments arranged by size:
1813 1567 1329
ApaLI G'TGCA_C
Cuts at: 3479 3479
Size: 4709
ApoI r'AATT_y
Cuts at: 261 328 464 1713 4062 4318 261
Size: 67 136 1249 2349 256 652
Fragments arranged by size:
2349 1249 652 256 136 67
AseI AT'TA_AT
Cuts at: 20 20
Size: 4709
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
AvaI C'yCGr_G
Cuts at: 1225 1729 1225
Size: 504 4205
BamHI G'GATC_C
Cuts at: 1734 1734
Size: 4709
BanI G'GyrC_C
Cuts at: 1725 2139 2387 2422 1725
Size: 414 248 35 4012
Fragments arranged by size:
4012 414 248 35
BanII G_rGCy'C
Cuts at: 1723 2753 1723
Size: 1030 3679
BbeI G_GCGC'C
Cuts at: 2143 2391 2143
Size: 248 4461
BbsI GAAGACnn'nnnn_
Cuts at: 1070 1674 1070
Size: 604 4105
BciVI GTATCCnnnnn_n'
Cuts at: 2607 3368 4709 2607
Size: 761 1341 2607
Fragments arranged by size:
2607 1341 761
BclI T'GATC_A
Cuts at: 933 1206 1353 2229 933
Size: 273 147 876 3413
Fragments arranged by size:
3413 876 273 147
BglII A'GATC_T
Cuts at: 2224 4261 2224
Size: 2037 2672
BlpI GC'TnA_GC
Cuts at: 1821 1821
Size: 4709
Bme1580I G_kGCm'C
Cuts at: 2334 2427 3483 2334
Size: 93 1056 3560
Fragments arranged by size:
3560 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1921 2002 2337 4481 928
Size: 993 81 335 2144 1156
Fragments arranged by size:
2144 1156 993 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 4709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 1077 1632 872
Size: 205 555 3949
Fragments arranged by size:
3949 555 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 1885 2764 3256 3554 1465
Size: 420 879 492 298 2620
Fragments arranged by size:
2620 879 492 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 1615 4413 1615
Size: 2798 1911
BsaAI yAC'GTr
Cuts at: 447 2062 447
Size: 1615 3094
BsaBI GATnn'nnATC
Cuts at: 6 692 1145 2223 4260 6
Size: 686 453 1078 2037 455
Fragments arranged by size:
2037 1078 686 455 453
BsaHI Gr'CG_yC
Cuts at: 2140 2388 3090 4586 2140
Size: 248 702 1496 2263
Fragments arranged by size:
2263 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1898 2419 3097 3371 3518 3929 1898
Size: 521 678 274 147 411 2678
Fragments arranged by size:
2678 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 609 618 621 859 1056 609
Size: 9 3 238 197 4262
Fragments arranged by size:
4262 238 197 9 3
BseYI C'CCAG_C
Cuts at: 2134 2892 3469 3965 2134
Size: 758 577 496 2878
Fragments arranged by size:
2878 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1272 2251 2297 3505 104
Size: 1168 979 46 1208 1308
Fragments arranged by size:
1308 1208 1168 979 46
BsiHKAI G_wGCw'C
Cuts at: 1383 1723 2501 2691 3483 1383
Size: 340 778 190 792 2609
Fragments arranged by size:
2609 792 778 340 190
BsmI GAATG_Cn'
Cuts at: 281 281
Size: 4709
BsmBI CGTCTCn'nnnn_
Cuts at: 713 713
Size: 4709
Bsp1286I G_dGCh'C
Cuts at: 1383 1723 2334 2427 2501 2691 2753 3483 1383
Size: 340 611 93 74 190 62 730 2609
Fragments arranged by size:
2609 730 611 340 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3929 3929
Size: 4709
BspHI T'CATG_A
Cuts at: 3885 3885
Size: 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1759 1973 2275 2656 1759
Size: 214 302 381 3812
Fragments arranged by size:
3812 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3001 3055 4539 4704 50
Size: 2951 54 1484 165 55
Fragments arranged by size:
2951 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 385 2621 385
Size: 2236 2473
BsrFI r'CCGG_y
Cuts at: 2707 2888 3097 2707
Size: 181 209 4319
Fragments arranged by size:
4319 209 181
BssSI C'ACGA_G
Cuts at: 2980 3338 2980
Size: 358 4351
BstAPI GCAn_nnn'nTGC
Cuts at: 2086 2086
Size: 4709
BstBI TT'CG_AA
Cuts at: 265 3070 265
Size: 2805 1904
BstYI r'GATC_y
Cuts at: 1 1140 1549 1734 2201 2224 2559 2805 3817
Size: 1139 409 185 467 23 335 246 1012
Cuts at: 3817 3903 3915 4261 1
Size: 86 12 346 449
Fragments arranged by size:
1139 1012 467 449 409 346 335 246
185 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 842 842
Size: 4709
BtgI C'CryG_G
Cuts at: 107 107
Size: 4709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1486 2659 2840 3003 1486
Size: 1173 181 163 3192
Fragments arranged by size:
3192 1173 181 163
BtsI GCAGTG_nn'
Cuts at: 528 1952 528
Size: 1424 3285
ClaI AT'CG_AT
Cuts at: 1268 1268
Size: 4709
DraI TTT'AAA
Cuts at: 1708 1708
Size: 4709
DrdI GACnn_nn'nnGTC
Cuts at: 2415 3273 2415
Size: 858 3851
EaeI y'GGCC_r
Cuts at: 831 1560 1653 2294 2468 2859 2886 4231 831
Size: 729 93 641 174 391 27 1345 1309
Fragments arranged by size:
1345 1309 729 641 391 174 93 27
EagI C'GGCC_G
Cuts at: 2294 2294
Size: 4709
EarI CTCTTCn'nnn_
Cuts at: 402 627 908 1247 2732 2942 402
Size: 225 281 339 1485 210 2169
Fragments arranged by size:
2169 1485 339 281 225 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1327 2917 3227 3373 4519 1327
Size: 1590 310 146 1146 1517
Fragments arranged by size:
1590 1517 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1721 1721
Size: 4709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 872 889 1064 1077 1199 1632 2534 2966
Size: 237 17 175 13 122 433 902 432
Cuts at: 2966 3713 3981 635
Size: 747 268 1363
Fragments arranged by size:
1363 902 747 433 432 268 237 175
122 17 13
EcoO109I rG'GnC_Cy
Cuts at: 1848 1848
Size: 4709
EcoRI G'AATT_C
Cuts at: 464 1713 464
Size: 1249 3460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4709
FspI TGC'GCA
Cuts at: 2490 2490
Size: 4709
HaeII r_GCGC'y
Cuts at: 2143 2391 3413 4392 2143
Size: 248 1022 979 2460
Fragments arranged by size:
2460 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 1051 1083 1482 1514 2551 2581 2583
Size: 32 352 32 399 32 1037 30 2
Cuts at: 2583 2613 667
Size: 30 2763
Fragments arranged by size:
2763 1037 399 352 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1641 1748 1641
Size: 107 4602
HindIII A'AGCT_T
Cuts at: 1160 1160
Size: 4709
KasI G'GCGC_C
Cuts at: 2139 2387 2139
Size: 248 4461
KpnI G_GTAC'C
Cuts at: 1729 1729
Size: 4709
MluI A'CGCG_T
Cuts at: 4142 4142
Size: 4709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3380 3564 3380
Size: 184 4525
MscI TGG'CCA
Cuts at: 833 1562 1655 2470 833
Size: 729 93 815 3072
Fragments arranged by size:
3072 815 729 93
MslI CAynn'nnrTG
Cuts at: 274 825 2698 2825 274
Size: 551 1873 127 2158
Fragments arranged by size:
2158 1873 551 127
MspA1I CmG'CkG
Cuts at: 1636 1820 2134 2494 3507 3752 1636
Size: 184 314 360 1013 245 2593
Fragments arranged by size:
2593 1013 360 314 245 184
NaeI GCC'GGC
Cuts at: 2890 2890
Size: 4709
NarI GG'CG_CC
Cuts at: 2140 2388 2140
Size: 248 4461
NcoI C'CATG_G
Cuts at: 107 107
Size: 4709
NgoMIV G'CCGG_C
Cuts at: 2888 2888
Size: 4709
NspI r_CATG'y
Cuts at: 739 1762 2262 3169 739
Size: 1023 500 907 2279
Fragments arranged by size:
2279 1023 907 500
PciI A'CATG_T
Cuts at: 735 3165 735
Size: 2430 2279
PflMI CCAn_nnn'nTGG
Cuts at: 829 1465 829
Size: 636 4073
PfoI T'CCnGG_A
Cuts at: 1585 1585
Size: 4709
PmeI GTTT'AAAC
Cuts at: 1708 1708
Size: 4709
PmlI CAC'GTG
Cuts at: 447 447
Size: 4709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4677
PshAI GACnn'nnGTC
Cuts at: 872 872
Size: 4709
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1461 1493 1461
Size: 32 4677
PstI C_TGCA'G
Cuts at: 1756 2441 1756
Size: 685 4024
PvuI CG_AT'CG
Cuts at: 104 1272 104
Size: 1168 3541
PvuII CAG'CTG
Cuts at: 2134 2494 2134
Size: 360 4349
RsrII CG'GwC_CG
Cuts at: 2904 2904
Size: 4709
SacI G_AGCT'C
Cuts at: 1723 1723
Size: 4709
SalI G'TCGA_C
Cuts at: 1746 1746
Size: 4709
SapI GCTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
SbfI CC_TGCA'GG
Cuts at: 1756 1756
Size: 4709
ScaI AGT'ACT
Cuts at: 816 4226 816
Size: 3410 1299
SfcI C'TryA_G
Cuts at: 33 1752 2437 3430 3621 33
Size: 1719 685 993 191 1121
Fragments arranged by size:
1719 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2141 2389 2141
Size: 248 4461
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
SmaI CCC'GGG
Cuts at: 1227 1731 1227
Size: 504 4205
SmlI C'TyrA_G
Cuts at: 1444 1864 2779 3271 3533 1444
Size: 420 915 492 262 2620
Fragments arranged by size:
2620 915 492 420 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4709
SphI G_CATG'C
Cuts at: 1762 2262 1762
Size: 500 4209
SspI AAT'ATT
Cuts at: 1018 1018
Size: 4709
StyI C'CwwG_G
Cuts at: 107 1843 2197 107
Size: 1736 354 2619
Fragments arranged by size:
2619 1736 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2292 2425 2884 3093 2292
Size: 133 459 209 3908
Fragments arranged by size:
3908 459 209 133
TatI w'GTAC_w
Cuts at: 814 1333 4224 814
Size: 519 2891 1299
Fragments arranged by size:
2891 1299 519
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1581 1613 4226 4258 1581
Size: 32 2613 32 2032
Fragments arranged by size:
2613 2032 32 32
Tth111I GACn'n_nGTC
Cuts at: 2506 2506
Size: 4709
XbaI T'CTAG_A
Cuts at: 1740 1740
Size: 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2204
Size: 4709
XmaI C'CCGG_G
Cuts at: 1225 1729 1225
Size: 504 4205
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsrGI BssHII
BstEII BstXI BstZ17I CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI