Restriction map for
pF1KA1428
(Circular) (Six-base) MAPSORT of: pF1KA1428.seq Check: 7296 from: 1 to: 5240
pF1KA1428 5240 bp
With 182 enzymes: SgfI *
October 16, 2009 16:19 ..
AccI GT'mk_AC
Cuts at: 1411 2130 2278 1411
Size: 719 148 4373
Fragments arranged by size:
4373 719 148
Acc65I G'GTAC_C
Cuts at: 2256 2256
Size: 5240
AclI AA'CG_TT
Cuts at: 5080 5080
Size: 5240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1607 3065 3497 4244 4512 655
Size: 952 1458 432 747 268 1383
Fragments arranged by size:
1458 1383 952 747 432 268
AflII C'TTAA_G
Cuts at: 1242 1242
Size: 5240
AflIII A'CryG_T
Cuts at: 2592 3696 4673 2592
Size: 1104 977 3159
Fragments arranged by size:
3159 1104 977
AgeI A'CCGG_T
Cuts at: 3628 3628
Size: 5240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 788 820 788
Size: 32 5208
AlwNI CAG_nnn'CTG
Cuts at: 2545 4112 2545
Size: 1567 3673
ApaLI G'TGCA_C
Cuts at: 4010 4010
Size: 5240
ApoI r'AATT_y
Cuts at: 778 916 933 1029 1416 2244 4593 4849 778
Size: 138 17 96 387 828 2349 256 1169
Fragments arranged by size:
2349 1169 828 387 256 138 96 17
AseI AT'TA_AT
Cuts at: 20 539 20
Size: 519 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5240
AvaI C'yCGr_G
Cuts at: 1700 2260 1700
Size: 560 4680
BamHI G'GATC_C
Cuts at: 1393 2265 1393
Size: 872 4368
BanI G'GyrC_C
Cuts at: 2256 2670 2918 2953 2256
Size: 414 248 35 4543
Fragments arranged by size:
4543 414 248 35
BanII G_rGCy'C
Cuts at: 358 2254 3284 358
Size: 1896 1030 2314
Fragments arranged by size:
2314 1896 1030
BbeI G_GCGC'C
Cuts at: 2674 2922 2674
Size: 248 4992
BbsI GAAGACnn'nnnn_
Cuts at: 1121 1121
Size: 5240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5206
BciVI GTATCCnnnnn_n'
Cuts at: 946 3138 3899 5240 946
Size: 2192 761 1341 946
Fragments arranged by size:
2192 1341 946 761
BclI T'GATC_A
Cuts at: 522 2760 522
Size: 2238 3002
BfrBI ATG'CAT
Cuts at: 240 645 240
Size: 405 4835
BglI GCCn_nnn'nGGC
Cuts at: 1532 1532
Size: 5240
BglII A'GATC_T
Cuts at: 471 2755 4792 471
Size: 2284 2037 919
Fragments arranged by size:
2284 2037 919
BlpI GC'TnA_GC
Cuts at: 394 2352 394
Size: 1958 3282
Bme1580I G_kGCm'C
Cuts at: 2865 2958 4014 2865
Size: 93 1056 4091
Fragments arranged by size:
4091 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 450 2452 2533 2868 5012 450
Size: 2002 81 335 2144 678
Fragments arranged by size:
2144 2002 678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 414 3607 414
Size: 3193 2047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1583 166
Size: 1417 3823
Bpu10I CC'TnA_GC
Cuts at: 2170 2170
Size: 5240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 3295 3787 4085 2416
Size: 879 492 298 3571
Fragments arranged by size:
3571 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 835 4944 835
Size: 4109 1131
BsaAI yAC'GTr
Cuts at: 2593 2593
Size: 5240
BsaBI GATnn'nnATC
Cuts at: 6 1655 2754 4791 6
Size: 1649 1099 2037 455
Fragments arranged by size:
2037 1649 1099 455
BsaHI Gr'CG_yC
Cuts at: 2671 2919 3621 5117 2671
Size: 248 702 1496 2794
Fragments arranged by size:
2794 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2429 2950 3628 3902 4049 4460 2429
Size: 521 678 274 147 411 3209
Fragments arranged by size:
3209 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 151
Size: 5240
BseYI C'CCAG_C
Cuts at: 2665 3423 4000 4496 2665
Size: 758 577 496 3409
Fragments arranged by size:
3409 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1253 293
Size: 960 4280
BsiEI CG_ry'CG
Cuts at: 104 2782 2828 4036 104
Size: 2678 46 1208 1308
Fragments arranged by size:
2678 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 358 2254 3032 3222 4014 358
Size: 1896 778 190 792 1584
Fragments arranged by size:
1896 1584 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 133 133
Size: 5240
Bsp1286I G_dGCh'C
Cuts at: 358 2254 2865 2958 3032 3222 3284 4014 358
Size: 1896 611 93 74 190 62 730 1584
Fragments arranged by size:
1896 1584 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4460 4460
Size: 5240
BspHI T'CATG_A
Cuts at: 525 1194 4416 525
Size: 669 3222 1349
Fragments arranged by size:
3222 1349 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2290 2504 2806 3187 2290
Size: 214 302 381 4343
Fragments arranged by size:
4343 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3532 3586 5070 5235 50
Size: 3482 54 1484 165 55
Fragments arranged by size:
3482 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 361 3152 361
Size: 2791 2449
BsrFI r'CCGG_y
Cuts at: 3238 3419 3628 3238
Size: 181 209 4850
Fragments arranged by size:
4850 209 181
BsrGI T'GTAC_A
Cuts at: 608 1210 608
Size: 602 4638
BssSI C'ACGA_G
Cuts at: 1271 1330 3511 3869 1271
Size: 59 2181 358 2642
Fragments arranged by size:
2642 2181 358 59
BstAPI GCAn_nnn'nTGC
Cuts at: 2617 2617
Size: 5240
BstBI TT'CG_AA
Cuts at: 3601 3601
Size: 5240
BstYI r'GATC_y
Cuts at: 1 471 1205 1393 1402 2265 2732 2755 3090
Size: 470 734 188 9 863 467 23 335
Cuts at: 3090 3336 4348 4434 4446 4792 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 863 734 470 467 449 346 335
246 188 86 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 1398 1398
Size: 5240
BtgI C'CryG_G
Cuts at: 1047 1073 1047
Size: 26 5214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3190 3371 3534 3190
Size: 181 163 4896
Fragments arranged by size:
4896 181 163
BtsI GCAGTG_nn'
Cuts at: 416 2483 416
Size: 2067 3173
DraI TTT'AAA
Cuts at: 302 462 1763 2239 302
Size: 160 1301 476 3303
Fragments arranged by size:
3303 1301 476 160
DrdI GACnn_nn'nnGTC
Cuts at: 2946 3804 2946
Size: 858 4382
EaeI y'GGCC_r
Cuts at: 1070 1342 1363 1506 1524 2825 2999 3390 3417
Size: 272 21 143 18 1301 174 391 27
Cuts at: 3417 4762 1070
Size: 1345 1548
Fragments arranged by size:
1548 1345 1301 391 272 174 143 27
21 18
EagI C'GGCC_G
Cuts at: 2825 2825
Size: 5240
EarI CTCTTCn'nnn_
Cuts at: 674 1193 2183 2212 3263 3473 674
Size: 519 990 29 1051 210 2441
Fragments arranged by size:
2441 1051 990 519 210 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3448 3758 3904 5050 3448
Size: 310 146 1146 3638
Fragments arranged by size:
3638 1146 310 146
EcoICRI GAG'CTC
Cuts at: 356 2252 356
Size: 1896 3344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 655 1583 1607 3065 3497 4244 4512 166
Size: 489 928 24 1458 432 747 268 894
Fragments arranged by size:
1458 928 894 747 489 432 268 24
EcoO109I rG'GnC_Cy
Cuts at: 2379 2379
Size: 5240
EcoRI G'AATT_C
Cuts at: 2244 2244
Size: 5240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3259 3259
Size: 5240
FspI TGC'GCA
Cuts at: 3021 3021
Size: 5240
HaeII r_GCGC'y
Cuts at: 1854 2674 2922 3944 4923 1854
Size: 820 248 1022 979 2171
Fragments arranged by size:
2171 1022 979 820 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1662 3082 3112 3114 3144 1630
Size: 32 1420 30 2 30 3726
Fragments arranged by size:
3726 1420 32 30 30 2
HincII GTy'rAC
Cuts at: 2279 2279
Size: 5240
KasI G'GCGC_C
Cuts at: 2670 2918 2670
Size: 248 4992
KpnI G_GTAC'C
Cuts at: 2260 2260
Size: 5240
MluI A'CGCG_T
Cuts at: 4673 4673
Size: 5240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 1944 3911 4095 233
Size: 1711 1967 184 1378
Fragments arranged by size:
1967 1711 1378 184
MscI TGG'CCA
Cuts at: 1072 1508 1526 3001 1072
Size: 436 18 1475 3311
Fragments arranged by size:
3311 1475 436 18
MslI CAynn'nnrTG
Cuts at: 1661 3229 3356 1661
Size: 1568 127 3545
Fragments arranged by size:
3545 1568 127
MspA1I CmG'CkG
Cuts at: 2351 2665 3025 4038 4283 2351
Size: 314 360 1013 245 3308
Fragments arranged by size:
3308 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3421 3421
Size: 5240
NarI GG'CG_CC
Cuts at: 2671 2919 2671
Size: 248 4992
NcoI C'CATG_G
Cuts at: 1073 1073
Size: 5240
NgoMIV G'CCGG_C
Cuts at: 3419 3419
Size: 5240
NsiI A_TGCA'T
Cuts at: 242 647 242
Size: 405 4835
NspI r_CATG'y
Cuts at: 720 1993 2293 2793 3700 720
Size: 1273 300 500 907 2260
Fragments arranged by size:
2260 1273 907 500 300
PciI A'CATG_T
Cuts at: 3696 3696
Size: 5240
PmeI GTTT'AAAC
Cuts at: 2239 2239
Size: 5240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4379 4411 4379
Size: 32 5208
PstI C_TGCA'G
Cuts at: 2287 2972 2287
Size: 685 4555
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5240
PvuII CAG'CTG
Cuts at: 2665 3025 2665
Size: 360 4880
RsrII CG'GwC_CG
Cuts at: 3435 3435
Size: 5240
SacI G_AGCT'C
Cuts at: 358 2254 358
Size: 1896 3344
SalI G'TCGA_C
Cuts at: 2277 2277
Size: 5240
SapI GCTCTTCn'nnn_
Cuts at: 3263 3473 3263
Size: 210 5030
SbfI CC_TGCA'GG
Cuts at: 2287 2287
Size: 5240
ScaI AGT'ACT
Cuts at: 4757 4757
Size: 5240
SfcI C'TryA_G
Cuts at: 33 2283 2968 3961 4152 33
Size: 2250 685 993 191 1121
Fragments arranged by size:
2250 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2672 2920 2672
Size: 248 4992
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5240
SmaI CCC'GGG
Cuts at: 1702 2262 1702
Size: 560 4680
SmlI C'TyrA_G
Cuts at: 1242 2395 3310 3802 4064 1242
Size: 1153 915 492 262 2418
Fragments arranged by size:
2418 1153 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5240
SphI G_CATG'C
Cuts at: 1993 2293 2793 1993
Size: 300 500 4440
Fragments arranged by size:
4440 500 300
SrfI GCCC'GGGC
Cuts at: 1702 1702
Size: 5240
SspI AAT'ATT
Cuts at: 792 792
Size: 5240
StuI AGG'CCT
Cuts at: 1067 1067
Size: 5240
StyI C'CwwG_G
Cuts at: 1073 1626 2374 2728 1073
Size: 553 748 354 3585
Fragments arranged by size:
3585 748 553 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2823 2956 3415 3624 2823
Size: 133 459 209 4439
Fragments arranged by size:
4439 459 209 133
TatI w'GTAC_w
Cuts at: 608 674 1210 1570 4755 608
Size: 66 536 360 3185 1093
Fragments arranged by size:
3185 1093 536 360 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 903 935 4757 4789 903
Size: 32 3822 32 1354
Fragments arranged by size:
3822 1354 32 32
Tth111I GACn'n_nGTC
Cuts at: 165 3037 165
Size: 2872 2368
XbaI T'CTAG_A
Cuts at: 1434 2271 1434
Size: 837 4403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2735 2735
Size: 5240
XmaI C'CCGG_G
Cuts at: 1700 2260 1700
Size: 560 4680
XmnI GAAnn'nnTTC
Cuts at: 503 503
Size: 5240
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BmgBI BmtI BsaXI BsiWI BsmI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SwaI XhoI ZraI