Restriction map for
pF1KA1404
(Circular) (Six-base) MAPSORT of: pF1KA1404.seq Check: 7532 from: 1 to: 8867
pF1KA1404 8867 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1388 5905 1388
Size: 4517 4350
Acc65I G'GTAC_C
Cuts at: 5883 5883
Size: 8867
AclI AA'CG_TT
Cuts at: 8707 8707
Size: 8867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 1784 1915 2194 5165 6692 7124 7477 8139
Size: 346 131 279 2971 1527 432 353 662
Cuts at: 8139 1438
Size: 2166
Fragments arranged by size:
2971 2166 1527 662 432 353 346 279
131
AfeI AGC'GCT
Cuts at: 5341 5341
Size: 8867
AflII C'TTAA_G
Cuts at: 3400 5098 3400
Size: 1698 7169
AflIII A'CryG_T
Cuts at: 3230 5500 5710 6219 8019 8300 3230
Size: 2270 210 509 1800 281 3797
Fragments arranged by size:
3797 2270 1800 509 281 210
AgeI A'CCGG_T
Cuts at: 4100 4344 4100
Size: 244 8623
AleI CACnn'nnGTG
Cuts at: 1069 4274 4527 1069
Size: 3205 253 5409
Fragments arranged by size:
5409 3205 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3634 4525 3634
Size: 891 7976
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3455 3487 4172 4204 3455
Size: 32 685 32 8118
Fragments arranged by size:
8118 685 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1305 1807 2103 2352 2512 2740 3070 3213 3378
Size: 502 296 249 160 228 330 143 165
Cuts at: 3378 3816 6172 7610 1305
Size: 438 2356 1438 2562
Fragments arranged by size:
2562 2356 1438 502 438 330 296 249
228 165 160 143
ApaLI G'TGCA_C
Cuts at: 1141 2319 4674 7705 1141
Size: 1178 2355 3031 2303
Fragments arranged by size:
3031 2355 2303 1178
ApoI r'AATT_y
Cuts at: 147 751 1437 1813 2212 5871 8220 8476 147
Size: 604 686 376 399 3659 2349 256 538
Fragments arranged by size:
3659 2349 686 604 538 399 376 256
AseI AT'TA_AT
Cuts at: 20 1853 20
Size: 1833 7034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8867
AvaI C'yCGr_G
Cuts at: 3773 5887 3773
Size: 2114 6753
AvrII C'CTAG_G
Cuts at: 268 268
Size: 8867
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2241 2274 2911 2944 2956 2989 3911 3944 2241
Size: 33 637 33 12 33 922 33 7164
Fragments arranged by size:
7164 922 637 33 33 33 33 12
BamHI G'GATC_C
Cuts at: 5892 5892
Size: 8867
BanI G'GyrC_C
Cuts at: 2876 3825 4097 4149 4977 5883 6297 6545 6580
Size: 949 272 52 828 906 414 248 35
Cuts at: 6580 2876
Size: 5163
Fragments arranged by size:
5163 949 906 828 414 272 248 52
35
BanII G_rGCy'C
Cuts at: 626 665 4697 5065 5796 5881 6911 626
Size: 39 4032 368 731 85 1030 2582
Fragments arranged by size:
4032 2582 1030 731 368 85 39
BbeI G_GCGC'C
Cuts at: 6301 6549 6301
Size: 248 8619
BbsI GAAGACnn'nnnn_
Cuts at: 126 553 870 1094 1301 2134 3023 3419 4880
Size: 427 317 224 207 833 889 396 1461
Cuts at: 4880 5153 126
Size: 273 3840
Fragments arranged by size:
3840 1461 889 833 427 396 317 273
224 207
BbvCI CC'TCA_GC
Cuts at: 2197 2956 2197
Size: 759 8108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1183 1217 4694 4728 1183
Size: 34 3477 34 5322
Fragments arranged by size:
5322 3477 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 836 1050 1209 3939 6765 7821 8867 836
Size: 214 159 2730 2826 1056 1046 836
Fragments arranged by size:
2826 2730 1056 1046 836 214 159
BclI T'GATC_A
Cuts at: 791 6387 791
Size: 5596 3271
BfrBI ATG'CAT
Cuts at: 1472 4061 4544 5274 1472
Size: 2589 483 730 5065
Fragments arranged by size:
5065 2589 730 483
BglI GCCn_nnn'nGGC
Cuts at: 2649 5660 2649
Size: 3011 5856
BglII A'GATC_T
Cuts at: 1533 6382 8419 1533
Size: 4849 2037 1981
Fragments arranged by size:
4849 2037 1981
BlpI GC'TnA_GC
Cuts at: 5064 5979 5064
Size: 915 7952
Bme1580I G_kGCm'C
Cuts at: 1045 1145 2323 3337 3830 4100 4154 4259 4337
Size: 100 1178 1014 493 270 54 105 78
Cuts at: 4337 4558 4678 4982 5710 6492 6585 7709 1045
Size: 221 120 304 728 782 93 1124 2203
Fragments arranged by size:
2203 1178 1124 1014 782 728 493 304
270 221 120 105 100 93 78 54
BmgBI CAC'GTC
Cuts at: 1659 1659
Size: 8867
BmrI ACTGGGnnnn_n'
Cuts at: 547 739 1817 2388 2738 3215 6079 6160 6495
Size: 192 1078 571 350 477 2864 81 335
Cuts at: 6495 8639 547
Size: 2144 775
Fragments arranged by size:
2864 2144 1078 775 571 477 350 335
192 81
BmtI G_CTAG'C
Cuts at: 1591 1591
Size: 8867
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 140 1743 4251 7234 140
Size: 1603 2508 2983 1773
Fragments arranged by size:
2983 2508 1773 1603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1657 2032 2335 2476 2486 2995 3400 5131
Size: 813 375 303 141 10 509 405 1731
Cuts at: 5131 844
Size: 4580
Fragments arranged by size:
4580 1731 813 509 405 375 303 141
10
Bpu10I CC'TnA_GC
Cuts at: 2197 2956 2197
Size: 759 8108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 3163 3280 4309 5364 5512 6043 6922 7636
Size: 2484 117 1029 1055 148 531 879 714
Cuts at: 7636 7934 679
Size: 298 1612
Fragments arranged by size:
2484 1612 1055 1029 879 714 531 298
148 117
BsaI GGTCTCn'nnnn_
Cuts at: 380 4768 8571 380
Size: 4388 3803 676
Fragments arranged by size:
4388 3803 676
BsaAI yAC'GTr
Cuts at: 5711 6220 5711
Size: 509 8358
BsaBI GATnn'nnATC
Cuts at: 6 2578 3571 5038 6381 8418 6
Size: 2572 993 1467 1343 2037 455
Fragments arranged by size:
2572 2037 1467 1343 993 455
BsaHI Gr'CG_yC
Cuts at: 3641 6298 6546 7248 8744 3641
Size: 2657 248 702 1496 3764
Fragments arranged by size:
3764 2657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4100 4344 6056 6577 7255 7666 7813 8087 4100
Size: 244 1712 521 678 411 147 274 4880
Fragments arranged by size:
4880 1712 678 521 411 274 244 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 1732 1762 2148 2178 835
Size: 30 867 30 386 30 7524
Fragments arranged by size:
7524 867 386 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 254 257 847 2557 2572 2575 2638 3685
Size: 127 3 590 1710 15 3 63 1047
Cuts at: 3685 5116 5857 127
Size: 1431 741 3137
Fragments arranged by size:
3137 1710 1431 1047 741 590 127 63
15 3 3
BseYI C'CCAG_C
Cuts at: 2372 2645 2774 5795 6292 7050 7715 8123 2372
Size: 273 129 3021 497 758 665 408 3116
Fragments arranged by size:
3116 3021 758 665 497 408 273 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1093 1417 2425 3007 3996 4312 4885 5554
Size: 177 324 1008 582 989 316 573 669
Cuts at: 5554 5626 916
Size: 72 4157
Fragments arranged by size:
4157 1008 989 669 582 573 324 316
177 72
BsiEI CG_ry'CG
Cuts at: 104 6409 6455 7685 104
Size: 6305 46 1230 1286
Fragments arranged by size:
6305 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 626 665 836 1145 2323 4678 5881 6659 6849
Size: 39 171 309 1178 2355 1203 778 190
Cuts at: 6849 7709 626
Size: 860 1784
Fragments arranged by size:
2355 1784 1203 1178 860 778 309 190
171 39
BsmI GAATG_Cn'
Cuts at: 2442 2442
Size: 8867
Bsp1286I G_dGCh'C
Cuts at: 626 665 836 1045 1145 2323 3337 3830 4100
Size: 39 171 209 100 1178 1014 493 270
Cuts at: 4100 4154 4259 4337 4558 4678 4697 4982 5065
Size: 54 105 78 221 120 19 285 83
Cuts at: 5065 5710 5796 5881 6492 6585 6659 6849 6911
Size: 645 86 85 611 93 74 190 62
Cuts at: 6911 7709 626
Size: 798 1784
Fragments arranged by size:
1784 1178 1014 798 645 611 493 285
270 221 209 190 171 120 105 100
93 86 85 83 78 74 62 54
39 19
BspHI T'CATG_A
Cuts at: 2261 2295 2390 7299 2261
Size: 34 95 4909 3829
Fragments arranged by size:
4909 3829 95 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2360 3029 3563 5917 6131 6433 6814 2360
Size: 669 534 2354 214 302 381 4413
Fragments arranged by size:
4413 2354 669 534 381 302 214
BsrBI CCG'CTC
Cuts at: 50 7159 7213 8697 8862 50
Size: 7109 54 1484 165 55
Fragments arranged by size:
7109 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3155 5664 6779 3155
Size: 2509 1115 5243
Fragments arranged by size:
5243 2509 1115
BsrFI r'CCGG_y
Cuts at: 4100 4344 6865 7046 4100
Size: 244 2521 181 5921
Fragments arranged by size:
5921 2521 244 181
BssSI C'ACGA_G
Cuts at: 5633 7138 7846 5633
Size: 1505 708 6654
Fragments arranged by size:
6654 1505 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3176 6244 3176
Size: 3068 5799
BstBI TT'CG_AA
Cuts at: 4466 7228 4466
Size: 2762 6105
BstEII G'GTnAC_C
Cuts at: 4311 4815 4311
Size: 504 8363
BstXI CCAn_nnnn'nTGG
Cuts at: 2313 2454 4276 2313
Size: 141 1822 6904
Fragments arranged by size:
6904 1822 141
BstYI r'GATC_y
Cuts at: 1 1533 1863 2951 3086 3375 4796 5054 5846
Size: 1532 330 1088 135 289 1421 258 792
Cuts at: 5846 5892 6359 6382 6717 6963 7269 7281 7367
Size: 46 467 23 335 246 306 12 86
Cuts at: 7367 8419 1
Size: 1052 449
Fragments arranged by size:
1532 1421 1088 1052 792 467 449 335
330 306 289 258 246 135 86 46
23 12
Bsu36I CC'TnA_GG
Cuts at: 1079 1306 2188 4996 1079
Size: 227 882 2808 4950
Fragments arranged by size:
4950 2808 882 227
BtgI C'CryG_G
Cuts at: 107 323 460 5696 107
Size: 216 137 5236 3278
Fragments arranged by size:
5236 3278 216 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6817 6998 7161 6817
Size: 181 163 8523
Fragments arranged by size:
8523 181 163
BtsI GCAGTG_nn'
Cuts at: 490 1199 6110 490
Size: 709 4911 3247
Fragments arranged by size:
4911 3247 709
DraI TTT'AAA
Cuts at: 5866 5866
Size: 8867
DraIII CAC_nnn'GTG
Cuts at: 419 3516 4409 419
Size: 3097 893 4877
Fragments arranged by size:
4877 3097 893
DrdI GACnn_nn'nnGTC
Cuts at: 6573 7917 6573
Size: 1344 7523
EaeI y'GGCC_r
Cuts at: 1869 2702 2996 3242 3818 4173 4746 5243 5330
Size: 833 294 246 576 355 573 497 87
Cuts at: 5330 5661 5819 6452 6626 7017 7044 8389 1869
Size: 331 158 633 174 391 27 1345 2347
Fragments arranged by size:
2347 1345 833 633 576 573 497 391
355 331 294 246 174 158 87 27
EagI C'GGCC_G
Cuts at: 6452 6452
Size: 8867
EarI CTCTTCn'nnn_
Cuts at: 302 965 1306 2624 2657 3604 3671 4044 5024
Size: 663 341 1318 33 947 67 373 980
Cuts at: 5024 5113 5279 5591 6890 7100 302
Size: 89 166 312 1299 210 2069
Fragments arranged by size:
2069 1318 1299 980 947 663 373 341
312 210 166 89 67 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 504 2206 2667 3979 7075 7817 7963 8677 504
Size: 1702 461 1312 3096 742 146 714 694
Fragments arranged by size:
3096 1702 1312 742 714 694 461 146
EcoICRI GAG'CTC
Cuts at: 624 663 5879 624
Size: 39 5216 3612
Fragments arranged by size:
5216 3612 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1438 1657 1784 1915 2032 2194 2335 2476
Size: 594 219 127 131 117 162 141 141
Cuts at: 2476 2486 2995 3400 5131 5165 6692 7124 7477
Size: 10 509 405 1731 34 1527 432 353
Cuts at: 7477 8139 844
Size: 662 1572
Fragments arranged by size:
1731 1572 1527 662 594 509 432 405
353 219 162 141 141 131 127 117
34 10
EcoNI CCTnn'n_nnAGG
Cuts at: 859 994 1704 1973 3094 3544 4442 859
Size: 135 710 269 1121 450 898 5284
Fragments arranged by size:
5284 1121 898 710 450 269 135
EcoO109I rG'GnC_Cy
Cuts at: 169 864 1157 1302 1963 2010 2246 2509 2642
Size: 695 293 145 661 47 236 263 133
Cuts at: 2642 3877 5103 5199 6006 169
Size: 1235 1226 96 807 3030
Fragments arranged by size:
3030 1235 1226 807 695 661 293 263
236 145 133 96 47
EcoRI G'AATT_C
Cuts at: 147 1437 2212 5871 147
Size: 1290 775 3659 3143
Fragments arranged by size:
3659 3143 1290 775
EcoRV GAT'ATC
Cuts at: 3746 3746
Size: 8867
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 643 1503 5358 5581 6886 643
Size: 860 3855 223 1305 2624
Fragments arranged by size:
3855 2624 1305 860 223
FspI TGC'GCA
Cuts at: 3610 6648 3610
Size: 3038 5829
HaeII r_GCGC'y
Cuts at: 4509 4659 5343 6301 6549 7779 8550 4509
Size: 150 684 958 248 1230 771 4826
Fragments arranged by size:
4826 1230 958 771 684 248 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1535 1567 4943 4975 5535 5567 6709 6739 6741
Size: 32 3376 32 560 32 1142 30 2
Cuts at: 6741 6771 1535
Size: 30 3631
Fragments arranged by size:
3631 3376 1142 560 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 963 4342 5906 963
Size: 3379 1564 3924
Fragments arranged by size:
3924 3379 1564
HindIII A'AGCT_T
Cuts at: 1287 3173 3935 1287
Size: 1886 762 6219
Fragments arranged by size:
6219 1886 762
KasI G'GCGC_C
Cuts at: 6297 6545 6297
Size: 248 8619
KpnI G_GTAC'C
Cuts at: 5887 5887
Size: 8867
MfeI C'AATT_G
Cuts at: 2118 2118
Size: 8867
MluI A'CGCG_T
Cuts at: 8300 8300
Size: 8867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3455 3773 7626 7810 3455
Size: 318 3853 184 4512
Fragments arranged by size:
4512 3853 318 184
MscI TGG'CCA
Cuts at: 1871 2704 3244 3820 4748 5245 5332 5663 6628
Size: 833 540 576 928 497 87 331 965
Cuts at: 6628 1871
Size: 4110
Fragments arranged by size:
4110 965 928 833 576 540 497 331
87
MslI CAynn'nnrTG
Cuts at: 131 1069 1148 1363 3760 3811 4031 4274 4527
Size: 938 79 215 2397 51 220 243 253
Cuts at: 4527 4808 5669 6856 6983 131
Size: 281 861 1187 127 2015
Fragments arranged by size:
2397 2015 1187 938 861 281 253 243
220 215 127 79 51
MspA1I CmG'CkG
Cuts at: 1584 2649 2740 3213 4460 4569 4586 4869 5022
Size: 1065 91 473 1247 109 17 283 153
Cuts at: 5022 5136 5978 6292 6652 7436 7681 1584
Size: 114 842 314 360 784 245 2770
Fragments arranged by size:
2770 1247 1065 842 784 473 360 314
283 245 153 114 109 91 17
NaeI GCC'GGC
Cuts at: 7048 7048
Size: 8867
NarI GG'CG_CC
Cuts at: 6298 6546 6298
Size: 248 8619
NcoI C'CATG_G
Cuts at: 107 323 460 5696 107
Size: 216 137 5236 3278
Fragments arranged by size:
5236 3278 216 137
NgoMIV G'CCGG_C
Cuts at: 7046 7046
Size: 8867
NheI G'CTAG_C
Cuts at: 1587 1587
Size: 8867
NsiI A_TGCA'T
Cuts at: 1474 4063 4546 5276 1474
Size: 2589 483 730 5065
Fragments arranged by size:
5065 2589 730 483
NspI r_CATG'y
Cuts at: 370 1476 3810 4009 5278 5504 5803 5920 6420
Size: 1106 2334 199 1269 226 299 117 500
Cuts at: 6420 8023 370
Size: 1603 1214
Fragments arranged by size:
2334 1603 1269 1214 1106 500 299 226
199 117
PciI A'CATG_T
Cuts at: 5500 8019 5500
Size: 2519 6348
PflMI CCAn_nnn'nTGG
Cuts at: 729 2103 2685 3378 3657 4546 5252 729
Size: 1374 582 693 279 889 706 4344
Fragments arranged by size:
4344 1374 889 706 693 582 279
PfoI T'CCnGG_A
Cuts at: 860 1661 1673 1682 1968 3011 4121 860
Size: 801 12 9 286 1043 1110 5606
Fragments arranged by size:
5606 1110 1043 801 286 12 9
PmeI GTTT'AAAC
Cuts at: 5866 5866
Size: 8867
PmlI CAC'GTG
Cuts at: 5711 5711
Size: 8867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7313 7345 7313
Size: 32 8835
PpuMI rG'GwC_Cy
Cuts at: 864 1963 2509 5199 864
Size: 1099 546 2690 4532
Fragments arranged by size:
4532 2690 1099 546
PshAI GACnn'nnGTC
Cuts at: 5204 5204
Size: 8867
PstI C_TGCA'G
Cuts at: 569 1025 2357 2847 3743 3973 4882 5914 6599
Size: 456 1332 490 896 230 909 1032 685
Cuts at: 6599 569
Size: 2837
Fragments arranged by size:
2837 1332 1032 909 896 685 490 456
230
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8867
PvuII CAG'CTG
Cuts at: 1584 2740 3213 4460 4869 5136 6292 6652 1584
Size: 1156 473 1247 409 267 1156 360 3799
Fragments arranged by size:
3799 1247 1156 1156 473 409 360 267
RsrII CG'GwC_CG
Cuts at: 7062 7062
Size: 8867
SacI G_AGCT'C
Cuts at: 626 665 5881 626
Size: 39 5216 3612
Fragments arranged by size:
5216 3612 39
SalI G'TCGA_C
Cuts at: 5904 5904
Size: 8867
SapI GCTCTTCn'nnn_
Cuts at: 3604 5113 5591 6890 7100 3604
Size: 1509 478 1299 210 5371
Fragments arranged by size:
5371 1509 1299 478 210
SbfI CC_TGCA'GG
Cuts at: 5914 5914
Size: 8867
ScaI AGT'ACT
Cuts at: 3535 8384 3535
Size: 4849 4018
SexAI A'CCwGG_T
Cuts at: 415 5402 415
Size: 4987 3880
SfcI C'TryA_G
Cuts at: 33 441 509 565 1021 1650 2353 2843 2920
Size: 408 68 56 456 629 703 490 77
Cuts at: 2920 3091 3739 3969 4878 5472 5910 6595 7563
Size: 171 648 230 909 594 438 685 968
Cuts at: 7563 7754 33
Size: 191 1146
Fragments arranged by size:
1146 968 909 703 685 648 629 594
490 456 438 408 230 191 171 77
68 56
SfoI GGC'GCC
Cuts at: 6299 6547 6299
Size: 248 8619
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8867
SgrAI Cr'CCGG_yG
Cuts at: 4100 4100
Size: 8867
SmaI CCC'GGG
Cuts at: 5889 5889
Size: 8867
SmlI C'TyrA_G
Cuts at: 658 3142 3259 3400 4324 5098 5343 5491 6022
Size: 2484 117 141 924 774 245 148 531
Cuts at: 6022 6937 7651 7913 658
Size: 915 714 262 1612
Fragments arranged by size:
2484 1612 924 915 774 714 531 262
245 148 141 117
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8867
SphI G_CATG'C
Cuts at: 370 5803 5920 6420 370
Size: 5433 117 500 2817
Fragments arranged by size:
5433 2817 500 117
SspI AAT'ATT
Cuts at: 4826 4826
Size: 8867
StuI AGG'CCT
Cuts at: 1648 4370 1648
Size: 2722 6145
StyI C'CwwG_G
Cuts at: 107 268 323 460 1478 1744 3669 3821 5696
Size: 161 55 137 1018 266 1925 152 1875
Cuts at: 5696 6001 6355 107
Size: 305 354 2619
Fragments arranged by size:
2619 1925 1875 1018 354 305 266 161
152 137 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2137 4286 4834 5527 6450 6583 7042 7251 2137
Size: 2149 548 693 923 133 459 209 3753
Fragments arranged by size:
3753 2149 923 693 548 459 209 133
TatI w'GTAC_w
Cuts at: 726 2360 3533 3791 4726 8382 726
Size: 1634 1173 258 935 3656 1211
Fragments arranged by size:
3656 1634 1211 1173 935 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2148 2180 8384 8416 2148
Size: 32 6204 32 2599
Fragments arranged by size:
6204 2599 32 32
Tth111I GACn'n_nGTC
Cuts at: 6664 6664
Size: 8867
XbaI T'CTAG_A
Cuts at: 5898 5898
Size: 8867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2725 4756 4828 6362 2725
Size: 2031 72 1534 5230
Fragments arranged by size:
5230 2031 1534 72
XmaI C'CCGG_G
Cuts at: 5887 5887
Size: 8867
XmnI GAAnn'nnTTC
Cuts at: 560 1694 2179 560
Size: 1134 485 7248
Fragments arranged by size:
7248 1134 485
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI ApaI AscI BsiWI BsmBI BspEI
BsrGI BssHII BstZ17I ClaI CspCI FseI FspAI HpaI
NdeI NotI NruI PacI PasI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SnaBI SrfI SwaI XhoI
ZraI