Restriction map for
pF1KA1390
(Circular) (Six-base) MAPSORT of: pF1KA1390.seq Check: 6569 from: 1 to: 4520
pF1KA1390 4520 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 916 916
Size: 4520
AccI GT'mk_AC
Cuts at: 772 1274 1558 772
Size: 502 284 3734
Fragments arranged by size:
3734 502 284
Acc65I G'GTAC_C
Cuts at: 1536 1536
Size: 4520
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 386 535 649 1348 2345 2777 3524 3792
Size: 45 149 114 699 997 432 747 268
Cuts at: 3792 341
Size: 1069
Fragments arranged by size:
1069 997 747 699 432 268 149 114
45
AflIII A'CryG_T
Cuts at: 1872 2976 3953 1872
Size: 1104 977 2439
Fragments arranged by size:
2439 1104 977
AgeI A'CCGG_T
Cuts at: 2908 2908
Size: 4520
AleI CACnn'nnGTG
Cuts at: 780 920 780
Size: 140 4380
AlwNI CAG_nnn'CTG
Cuts at: 139 966 1021 1825 3392 139
Size: 827 55 804 1567 1267
Fragments arranged by size:
1567 1267 827 804 55
ApaLI G'TGCA_C
Cuts at: 3290 3290
Size: 4520
ApoI r'AATT_y
Cuts at: 1524 3873 4129 1524
Size: 2349 256 1915
Fragments arranged by size:
2349 1915 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 778 1540 778
Size: 762 3758
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 799 832 799
Size: 33 4487
BamHI G'GATC_C
Cuts at: 129 461 1545 129
Size: 332 1084 3104
Fragments arranged by size:
3104 1084 332
BanI G'GyrC_C
Cuts at: 1536 1950 2198 2233 1536
Size: 414 248 35 3823
Fragments arranged by size:
3823 414 248 35
BanII G_rGCy'C
Cuts at: 605 1534 2564 605
Size: 929 1030 2561
Fragments arranged by size:
2561 1030 929
BbeI G_GCGC'C
Cuts at: 1954 2202 1954
Size: 248 4272
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3179 4520 2418
Size: 761 1341 2418
Fragments arranged by size:
2418 1341 761
BclI T'GATC_A
Cuts at: 587 2040 587
Size: 1453 3067
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 330 1632 330
Size: 1302 3218
Bme1580I G_kGCm'C
Cuts at: 2145 2238 3294 2145
Size: 93 1056 3371
Fragments arranged by size:
3371 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1732 1813 2148 4292 1732
Size: 81 335 2144 1960
Fragments arranged by size:
2144 1960 335 81
BmtI G_CTAG'C
Cuts at: 329 329
Size: 4520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 903 988 1250 903
Size: 85 262 4173
Fragments arranged by size:
4173 262 85
Bpu10I CC'TnA_GC
Cuts at: 434 1025 434
Size: 591 3929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1696 2575 3067 3365 325
Size: 1371 879 492 298 1480
Fragments arranged by size:
1480 1371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 964 4224 964
Size: 3260 1260
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 755 1458 1951 2199 2901 4397 755
Size: 703 493 248 702 1496 878
Fragments arranged by size:
1496 878 703 702 493 248
BsaWI w'CCGG_w
Cuts at: 1709 2230 2908 3182 3329 3740 1709
Size: 521 678 274 147 411 2489
Fragments arranged by size:
2489 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1147 1387 1457 1147
Size: 240 70 4210
Fragments arranged by size:
4210 240 70
BseYI C'CCAG_C
Cuts at: 983 1329 1355 1461 1945 2703 3280 3776 983
Size: 346 26 106 484 758 577 496 1727
Fragments arranged by size:
1727 758 577 496 484 346 106 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 881 1141 804
Size: 77 260 4183
Fragments arranged by size:
4183 260 77
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3316 104
Size: 1958 46 1208 1308
Fragments arranged by size:
1958 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 209 505 605 1534 2312 2502 3294 209
Size: 296 100 929 778 190 792 1435
Fragments arranged by size:
1435 929 792 778 296 190 100
BsmI GAATG_Cn'
Cuts at: 1505 1505
Size: 4520
BsmBI CGTCTCn'nnnn_
Cuts at: 762 762
Size: 4520
Bsp1286I G_dGCh'C
Cuts at: 209 505 605 1534 2145 2238 2312 2502 2564
Size: 296 100 929 611 93 74 190 62
Cuts at: 2564 3294 209
Size: 730 1435
Fragments arranged by size:
1435 929 730 611 296 190 100 93
74 62
BspEI T'CCGG_A
Cuts at: 3740 3740
Size: 4520
BspHI T'CATG_A
Cuts at: 533 3696 533
Size: 3163 1357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 806 916 1260 1570 1784 2086 2467 806
Size: 110 344 310 214 302 381 2859
Fragments arranged by size:
2859 381 344 310 302 214 110
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 704 2432 704
Size: 1728 2792
BsrFI r'CCGG_y
Cuts at: 2518 2699 2908 2518
Size: 181 209 4130
Fragments arranged by size:
4130 209 181
BssSI C'ACGA_G
Cuts at: 934 1319 1492 2791 3149 934
Size: 385 173 1299 358 2305
Fragments arranged by size:
2305 1299 385 358 173
BstAPI GCAn_nnn'nTGC
Cuts at: 232 1404 1897 232
Size: 1172 493 2855
Fragments arranged by size:
2855 1172 493
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 729 729
Size: 4520
BstYI r'GATC_y
Cuts at: 1 129 461 1545 2012 2035 2370 2616 3628
Size: 128 332 1084 467 23 335 246 1012
Cuts at: 3628 3714 3726 4072 1
Size: 86 12 346 449
Fragments arranged by size:
1084 1012 467 449 346 335 332 246
128 86 23 12
BstZ17I GTA'TAC
Cuts at: 773 773
Size: 4520
Bsu36I CC'TnA_GG
Cuts at: 125 212 848 125
Size: 87 636 3797
Fragments arranged by size:
3797 636 87
BtgI C'CryG_G
Cuts at: 107 819 977 996 107
Size: 712 158 19 3631
Fragments arranged by size:
3631 712 158 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2470 2651 2814 2470
Size: 181 163 4176
Fragments arranged by size:
4176 181 163
BtsI GCAGTG_nn'
Cuts at: 358 792 1429 1763 358
Size: 434 637 334 3115
Fragments arranged by size:
3115 637 434 334
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DraIII CAC_nnn'GTG
Cuts at: 1164 1164
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 2226 3084 2226
Size: 858 3662
EaeI y'GGCC_r
Cuts at: 719 2105 2279 2670 2697 4042 719
Size: 1386 174 391 27 1345 1197
Fragments arranged by size:
1386 1345 1197 391 174 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3038 3184 4330 2728
Size: 310 146 1146 2918
Fragments arranged by size:
2918 1146 310 146
EcoICRI GAG'CTC
Cuts at: 603 1532 603
Size: 929 3591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 386 535 649 903 988 1250 1348 2345
Size: 45 149 114 254 85 262 98 997
Cuts at: 2345 2777 3524 3792 341
Size: 432 747 268 1069
Fragments arranged by size:
1069 997 747 432 268 262 254 149
114 98 85 45
EcoNI CCTnn'n_nnAGG
Cuts at: 470 1219 470
Size: 749 3771
EcoO109I rG'GnC_Cy
Cuts at: 422 1225 1422 1659 422
Size: 803 197 237 3283
Fragments arranged by size:
3283 803 237 197
EcoRI G'AATT_C
Cuts at: 1524 1524
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1110 2539 1110
Size: 1429 3091
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 1954 2202 3224 4203 1954
Size: 248 1022 979 2271
Fragments arranged by size:
2271 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2362 2392 2394 2424 2362
Size: 30 2 30 4458
Fragments arranged by size:
4458 30 30 2
HincII GTy'rAC
Cuts at: 1559 1559
Size: 4520
HindIII A'AGCT_T
Cuts at: 272 272
Size: 4520
KasI G'GCGC_C
Cuts at: 1950 2198 1950
Size: 248 4272
KpnI G_GTAC'C
Cuts at: 1540 1540
Size: 4520
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3191 3375 3191
Size: 184 4336
MscI TGG'CCA
Cuts at: 721 2281 721
Size: 1560 2960
MslI CAynn'nnrTG
Cuts at: 780 920 2509 2636 780
Size: 140 1589 127 2664
Fragments arranged by size:
2664 1589 140 127
MspA1I CmG'CkG
Cuts at: 888 979 987 1021 1178 1266 1329 1631 1945
Size: 91 8 34 157 88 63 302 314
Cuts at: 1945 2305 3318 3563 888
Size: 360 1013 245 1845
Fragments arranged by size:
1845 1013 360 314 302 245 157 91
88 63 34 8
NaeI GCC'GGC
Cuts at: 2701 2701
Size: 4520
NarI GG'CG_CC
Cuts at: 1951 2199 1951
Size: 248 4272
NcoI C'CATG_G
Cuts at: 107 819 107
Size: 712 3808
NgoMIV G'CCGG_C
Cuts at: 2699 2699
Size: 4520
NheI G'CTAG_C
Cuts at: 325 325
Size: 4520
NspI r_CATG'y
Cuts at: 389 1573 2073 2980 389
Size: 1184 500 907 1929
Fragments arranged by size:
1929 1184 907 500
PasI CC'CwG_GG
Cuts at: 1215 1215
Size: 4520
PciI A'CATG_T
Cuts at: 2976 2976
Size: 4520
PflMI CCAn_nnn'nTGG
Cuts at: 139 352 891 139
Size: 213 539 3768
Fragments arranged by size:
3768 539 213
PfoI T'CCnGG_A
Cuts at: 965 965
Size: 4520
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4488
PpuMI rG'GwC_Cy
Cuts at: 422 1422 422
Size: 1000 3520
PshAI GACnn'nnGTC
Cuts at: 1420 1420
Size: 4520
PstI C_TGCA'G
Cuts at: 159 1271 1307 1436 1567 2252 159
Size: 1112 36 129 131 685 2427
Fragments arranged by size:
2427 1112 685 131 129 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 888 987 1021 1178 1266 1945 2305 888
Size: 99 34 157 88 679 360 3103
Fragments arranged by size:
3103 679 360 157 99 88 34
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 605 1534 605
Size: 929 3591
SacII CC_GC'GG
Cuts at: 980 980
Size: 4520
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 155 1126 1267 1303 1432 1563 2248 3241
Size: 122 971 141 36 129 131 685 993
Cuts at: 3241 3432 33
Size: 191 1121
Fragments arranged by size:
1121 993 971 685 191 141 131 129
122 36
SfoI GGC'GCC
Cuts at: 1952 2200 1952
Size: 248 4272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 304 1675 2590 3082 3344 304
Size: 1371 915 492 262 1480
Fragments arranged by size:
1480 1371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 389 1573 2073 389
Size: 1184 500 2836
Fragments arranged by size:
2836 1184 500
StuI AGG'CCT
Cuts at: 947 947
Size: 4520
StyI C'CwwG_G
Cuts at: 107 351 466 819 1654 2008 107
Size: 244 115 353 835 354 2619
Fragments arranged by size:
2619 835 354 353 244 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 501 511 2103 2236 2695 2904 501
Size: 10 1592 133 459 209 2117
Fragments arranged by size:
2117 1592 459 209 133 10
TatI w'GTAC_w
Cuts at: 4035 4035
Size: 4520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 4488
Tth111I GACn'n_nGTC
Cuts at: 2317 2317
Size: 4520
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2015 2015
Size: 4520
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BsaXI
BsiWI BsrGI BssHII BstEII ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI