Restriction map for
pF1KA1385
(Circular) (Six-base) MAPSORT of: pF1KA1385.seq Check: 9290 from: 1 to: 5321
pF1KA1385 5321 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1848 2017 1848
Size: 169 5152
AccI GT'mk_AC
Cuts at: 2136 2359 2136
Size: 223 5098
Acc65I G'GTAC_C
Cuts at: 2337 2337
Size: 5321
AclI AA'CG_TT
Cuts at: 5161 5161
Size: 5321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 893 1448 1667 3146 3578 4325 4593 171
Size: 722 555 219 1479 432 747 268 899
Fragments arranged by size:
1479 899 747 722 555 432 268 219
AflIII A'CryG_T
Cuts at: 2673 3777 4754 2673
Size: 1104 977 3240
Fragments arranged by size:
3240 1104 977
AgeI A'CCGG_T
Cuts at: 3709 3709
Size: 5321
AleI CACnn'nnGTG
Cuts at: 1039 1039
Size: 5321
AlwNI CAG_nnn'CTG
Cuts at: 1863 2626 4193 1863
Size: 763 1567 2991
Fragments arranged by size:
2991 1567 763
ApaI G_GGCC'C
Cuts at: 1543 1543
Size: 5321
ApaLI G'TGCA_C
Cuts at: 4091 4091
Size: 5321
ApoI r'AATT_y
Cuts at: 1445 2325 4674 4930 1445
Size: 880 2349 256 1836
Fragments arranged by size:
2349 1836 880 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5321
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
AvaI C'yCGr_G
Cuts at: 2341 2341
Size: 5321
BamHI G'GATC_C
Cuts at: 2058 2346 2058
Size: 288 5033
BanI G'GyrC_C
Cuts at: 2337 2751 2999 3034 2337
Size: 414 248 35 4624
Fragments arranged by size:
4624 414 248 35
BanII G_rGCy'C
Cuts at: 1543 2273 2335 3365 1543
Size: 730 62 1030 3499
Fragments arranged by size:
3499 1030 730 62
BbeI G_GCGC'C
Cuts at: 2755 3003 2755
Size: 248 5073
BbsI GAAGACnn'nnnn_
Cuts at: 209 731 1056 1549 209
Size: 522 325 493 3981
Fragments arranged by size:
3981 522 493 325
BbvCI CC'TCA_GC
Cuts at: 895 895
Size: 5321
BciVI GTATCCnnnnn_n'
Cuts at: 1839 3219 3980 5321 1839
Size: 1380 761 1341 1839
Fragments arranged by size:
1839 1380 1341 761
BclI T'GATC_A
Cuts at: 2841 2841
Size: 5321
BglII A'GATC_T
Cuts at: 228 2836 4873 228
Size: 2608 2037 676
Fragments arranged by size:
2608 2037 676
BlpI GC'TnA_GC
Cuts at: 2433 2433
Size: 5321
Bme1580I G_kGCm'C
Cuts at: 1543 2175 2946 3039 4095 1543
Size: 632 771 93 1056 2769
Fragments arranged by size:
2769 1056 771 632 93
BmrI ACTGGGnnnn_n'
Cuts at: 2533 2614 2949 5093 2533
Size: 81 335 2144 2761
Fragments arranged by size:
2761 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3688 3688
Size: 5321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 383 1129 380
Size: 3 746 4572
Fragments arranged by size:
4572 746 3
Bpu10I CC'TnA_GC
Cuts at: 895 926 895
Size: 31 5290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 1837 2497 3376 3868 4166 1160
Size: 677 660 879 492 298 2315
Fragments arranged by size:
2315 879 677 660 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5025 5025
Size: 5321
BsaAI yAC'GTr
Cuts at: 616 2265 2674 616
Size: 1649 409 3263
Fragments arranged by size:
3263 1649 409
BsaBI GATnn'nnATC
Cuts at: 6 2835 4872 6
Size: 2829 2037 455
Fragments arranged by size:
2829 2037 455
BsaHI Gr'CG_yC
Cuts at: 2752 3000 3702 5198 2752
Size: 248 702 1496 2875
Fragments arranged by size:
2875 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1119 2510 3031 3709 3983 4130 4541 1119
Size: 1391 521 678 274 147 411 1899
Fragments arranged by size:
1899 1391 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 1335 1365 874
Size: 30 431 30 4830
Fragments arranged by size:
4830 431 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 683 683
Size: 5321
BseYI C'CCAG_C
Cuts at: 1212 2746 3504 4081 4577 1212
Size: 1534 758 577 496 1956
Fragments arranged by size:
1956 1534 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 2015 1005
Size: 1010 4311
BsiEI CG_ry'CG
Cuts at: 104 116 2863 2909 4117 104
Size: 12 2747 46 1208 1308
Fragments arranged by size:
2747 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 875 927 1353 2273 2335 3113 3303 4095 875
Size: 52 426 920 62 778 190 792 2101
Fragments arranged by size:
2101 920 792 778 426 190 62 52
BsmI GAATG_Cn'
Cuts at: 571 571
Size: 5321
Bsp1286I G_dGCh'C
Cuts at: 875 927 1353 1543 2175 2273 2335 2946 3039
Size: 52 426 190 632 98 62 611 93
Cuts at: 3039 3113 3303 3365 4095 875
Size: 74 190 62 730 2101
Fragments arranged by size:
2101 730 632 611 426 190 190 98
93 74 62 62 52
BspEI T'CCGG_A
Cuts at: 4541 4541
Size: 5321
BspHI T'CATG_A
Cuts at: 4497 4497
Size: 5321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 1848 2017 2371 2585 2887 3268 545
Size: 1303 169 354 214 302 381 2598
Fragments arranged by size:
2598 1303 381 354 302 214 169
BsrBI CCG'CTC
Cuts at: 50 3613 3667 5151 5316 50
Size: 3563 54 1484 165 55
Fragments arranged by size:
3563 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 455 824 3233 455
Size: 369 2409 2543
Fragments arranged by size:
2543 2409 369
BsrFI r'CCGG_y
Cuts at: 3319 3500 3709 3319
Size: 181 209 4931
Fragments arranged by size:
4931 209 181
BssSI C'ACGA_G
Cuts at: 383 857 878 920 3592 3950 383
Size: 474 21 42 2672 358 1754
Fragments arranged by size:
2672 1754 474 358 42 21
BstAPI GCAn_nnn'nTGC
Cuts at: 826 2698 826
Size: 1872 3449
BstBI TT'CG_AA
Cuts at: 3682 3682
Size: 5321
BstEII G'GTnAC_C
Cuts at: 1720 2004 1720
Size: 284 5037
BstXI CCAn_nnnn'nTGG
Cuts at: 2168 2168
Size: 5321
BstYI r'GATC_y
Cuts at: 1 228 240 556 1886 2058 2346 2813 2836
Size: 227 12 316 1330 172 288 467 23
Cuts at: 2836 3171 3417 4429 4515 4527 4873 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1330 1012 467 449 346 335 316 288
246 227 172 86 23 12 12
BstZ17I GTA'TAC
Cuts at: 2137 2137
Size: 5321
BtgI C'CryG_G
Cuts at: 107 1832 107
Size: 1725 3596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1039 3271 3452 3615 1039
Size: 2232 181 163 2745
Fragments arranged by size:
2745 2232 181 163
BtsI GCAGTG_nn'
Cuts at: 216 2226 2564 216
Size: 2010 338 2973
Fragments arranged by size:
2973 2010 338
DraI TTT'AAA
Cuts at: 2320 2320
Size: 5321
DrdI GACnn_nn'nnGTC
Cuts at: 3027 3885 3027
Size: 858 4463
EaeI y'GGCC_r
Cuts at: 1462 1488 1520 2065 2906 3080 3471 3498 4843
Size: 26 32 545 841 174 391 27 1345
Cuts at: 4843 1462
Size: 1940
Fragments arranged by size:
1940 1345 841 545 391 174 32 27
26
EagI C'GGCC_G
Cuts at: 2906 2906
Size: 5321
EarI CTCTTCn'nnn_
Cuts at: 743 752 1700 3344 3554 743
Size: 9 948 1644 210 2510
Fragments arranged by size:
2510 1644 948 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3529 3839 3985 5131 3529
Size: 310 146 1146 3719
Fragments arranged by size:
3719 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2271 2333 2271
Size: 62 5259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 380 383 893 1129 1448 1667 3146 3578
Size: 209 3 510 236 319 219 1479 432
Cuts at: 3578 4325 4593 171
Size: 747 268 899
Fragments arranged by size:
1479 899 747 510 432 319 268 236
219 209 3
EcoO109I rG'GnC_Cy
Cuts at: 1540 2460 1540
Size: 920 4401
EcoRI G'AATT_C
Cuts at: 1445 2325 1445
Size: 880 4441
EcoRV GAT'ATC
Cuts at: 1032 1476 1032
Size: 444 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3340 3340
Size: 5321
FspI TGC'GCA
Cuts at: 2218 3102 2218
Size: 884 4437
HaeII r_GCGC'y
Cuts at: 2755 3003 4025 5004 2755
Size: 248 1022 979 3072
Fragments arranged by size:
3072 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 874 906 2187 2219 2229 2261 3163
Size: 32 58 32 1281 32 10 32 902
Cuts at: 3163 3193 3195 3225 784
Size: 30 2 30 2880
Fragments arranged by size:
2880 1281 902 58 32 32 32 32
30 30 10 2
HincII GTy'rAC
Cuts at: 1622 2360 1622
Size: 738 4583
KasI G'GCGC_C
Cuts at: 2751 2999 2751
Size: 248 5073
KpnI G_GTAC'C
Cuts at: 2341 2341
Size: 5321
MluI A'CGCG_T
Cuts at: 4754 4754
Size: 5321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 138 3992 4176 138
Size: 3854 184 1283
Fragments arranged by size:
3854 1283 184
MscI TGG'CCA
Cuts at: 1522 3082 1522
Size: 1560 3761
MslI CAynn'nnrTG
Cuts at: 1039 3310 3437 1039
Size: 2271 127 2923
Fragments arranged by size:
2923 2271 127
MspA1I CmG'CkG
Cuts at: 2432 2746 3106 4119 4364 2432
Size: 314 360 1013 245 3389
Fragments arranged by size:
3389 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3502 3502
Size: 5321
NarI GG'CG_CC
Cuts at: 2752 3000 2752
Size: 248 5073
NcoI C'CATG_G
Cuts at: 107 1832 107
Size: 1725 3596
NgoMIV G'CCGG_C
Cuts at: 3500 3500
Size: 5321
NspI r_CATG'y
Cuts at: 489 1062 2217 2374 2874 3781 489
Size: 573 1155 157 500 907 2029
Fragments arranged by size:
2029 1155 907 573 500 157
PciI A'CATG_T
Cuts at: 3777 3777
Size: 5321
PfoI T'CCnGG_A
Cuts at: 1106 1106
Size: 5321
PmeI GTTT'AAAC
Cuts at: 2320 2320
Size: 5321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 4460
Size: 32 5289
PspOMI G'GGCC_C
Cuts at: 1539 1539
Size: 5321
PstI C_TGCA'G
Cuts at: 819 2368 3053 819
Size: 1549 685 3087
Fragments arranged by size:
3087 1549 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5321
PvuII CAG'CTG
Cuts at: 2746 3106 2746
Size: 360 4961
RsrII CG'GwC_CG
Cuts at: 3516 3516
Size: 5321
SacI G_AGCT'C
Cuts at: 2273 2335 2273
Size: 62 5259
SalI G'TCGA_C
Cuts at: 2358 2358
Size: 5321
SapI GCTCTTCn'nnn_
Cuts at: 3344 3554 3344
Size: 210 5111
SbfI CC_TGCA'GG
Cuts at: 2368 2368
Size: 5321
ScaI AGT'ACT
Cuts at: 4838 4838
Size: 5321
SfcI C'TryA_G
Cuts at: 33 815 947 1568 2364 3049 4042 4233 33
Size: 782 132 621 796 685 993 191 1121
Fragments arranged by size:
1121 993 796 782 685 621 191 132
SfoI GGC'GCC
Cuts at: 2753 3001 2753
Size: 248 5073
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
SmaI CCC'GGG
Cuts at: 2343 2343
Size: 5321
SmlI C'TyrA_G
Cuts at: 1175 1852 2476 3391 3883 4145 1175
Size: 677 624 915 492 262 2351
Fragments arranged by size:
2351 915 677 624 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5321
SphI G_CATG'C
Cuts at: 489 2217 2374 2874 489
Size: 1728 157 500 2936
Fragments arranged by size:
2936 1728 500 157
StuI AGG'CCT
Cuts at: 498 498
Size: 5321
StyI C'CwwG_G
Cuts at: 107 1037 1832 2167 2455 2809 107
Size: 930 795 335 288 354 2619
Fragments arranged by size:
2619 930 795 354 335 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 244 2904 3037 3496 3705 130
Size: 114 2660 133 459 209 1746
Fragments arranged by size:
2660 1746 459 209 133 114
TatI w'GTAC_w
Cuts at: 1380 2179 4836 1380
Size: 799 2657 1865
Fragments arranged by size:
2657 1865 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 956 988 4838 4870 956
Size: 32 3850 32 1407
Fragments arranged by size:
3850 1407 32 32
Tth111I GACn'n_nGTC
Cuts at: 3118 3118
Size: 5321
XbaI T'CTAG_A
Cuts at: 2352 2352
Size: 5321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1734 2816 1734
Size: 1082 4239
XmaI C'CCGG_G
Cuts at: 2341 2341
Size: 5321
XmnI GAAnn'nnTTC
Cuts at: 569 569
Size: 5321
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI