Restriction map for
pF1KA1381
(Circular) (Six-base) MAPSORT of: pF1KA1381.seq Check: 348 from: 1 to: 6053
pF1KA1381 6053 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 633 1085 633
Size: 452 5601
AccI GT'mk_AC
Cuts at: 3091 3091
Size: 6053
Acc65I G'GTAC_C
Cuts at: 272 3069 272
Size: 2797 3256
AclI AA'CG_TT
Cuts at: 5893 5893
Size: 6053
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 925 1702 2011 3878 4310 4663 5325 157
Size: 768 777 309 1867 432 353 662 885
Fragments arranged by size:
1867 885 777 768 662 432 353 309
AflIII A'CryG_T
Cuts at: 1175 1517 2996 3405 5205 5486 1175
Size: 342 1479 409 1800 281 1742
Fragments arranged by size:
1800 1742 1479 409 342 281
AhdI GACnn_n'nnGTC
Cuts at: 2977 2997 2977
Size: 20 6033
AleI CACnn'nnGTG
Cuts at: 848 848
Size: 6053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 712 932 1925 712
Size: 220 993 4840
Fragments arranged by size:
4840 993 220
AlwNI CAG_nnn'CTG
Cuts at: 1368 1392 1958 1968 2287 2956 3358 4796 1368
Size: 24 566 10 319 669 402 1438 2625
Fragments arranged by size:
2625 1438 669 566 402 319 24 10
ApaI G_GGCC'C
Cuts at: 417 417
Size: 6053
ApaLI G'TGCA_C
Cuts at: 4891 4891
Size: 6053
ApoI r'AATT_y
Cuts at: 485 2267 3057 5406 5662 485
Size: 1782 790 2349 256 876
Fragments arranged by size:
2349 1782 876 790 256
AseI AT'TA_AT
Cuts at: 20 2375 20
Size: 2355 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6053
AvaI C'yCGr_G
Cuts at: 3073 3073
Size: 6053
BamHI G'GATC_C
Cuts at: 1169 3078 1169
Size: 1909 4144
BanI G'GyrC_C
Cuts at: 272 3069 3483 3731 3766 272
Size: 2797 414 248 35 2559
Fragments arranged by size:
2797 2559 414 248 35
BanII G_rGCy'C
Cuts at: 417 508 1439 2023 2052 3067 4097 417
Size: 91 931 584 29 1015 1030 2373
Fragments arranged by size:
2373 1030 1015 931 584 91 29
BbeI G_GCGC'C
Cuts at: 3487 3735 3487
Size: 248 5805
BbsI GAAGACnn'nnnn_
Cuts at: 1193 2996 1193
Size: 1803 4250
BciVI GTATCCnnnnn_n'
Cuts at: 3951 5007 6053 3951
Size: 1056 1046 3951
Fragments arranged by size:
3951 1056 1046
BclI T'GATC_A
Cuts at: 3573 3573
Size: 6053
BfrBI ATG'CAT
Cuts at: 1838 1838
Size: 6053
BglI GCCn_nnn'nGGC
Cuts at: 118 349 386 391 811 118
Size: 231 37 5 420 5360
Fragments arranged by size:
5360 420 231 37 5
BglII A'GATC_T
Cuts at: 497 3568 5605 497
Size: 3071 2037 945
Fragments arranged by size:
3071 2037 945
BlpI GC'TnA_GC
Cuts at: 3165 3165
Size: 6053
Bme1580I G_kGCm'C
Cuts at: 417 1030 1984 3678 3771 4895 417
Size: 613 954 1694 93 1124 1575
Fragments arranged by size:
1694 1575 1124 954 613 93
BmgBI CAC'GTC
Cuts at: 2999 2999
Size: 6053
BmrI ACTGGGnnnn_n'
Cuts at: 2196 3265 3346 3681 5825 2196
Size: 1069 81 335 2144 2424
Fragments arranged by size:
2424 2144 1069 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 791 2484 4420 791
Size: 1693 1936 2424
Fragments arranged by size:
2424 1936 1693
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 522 562 1012 1348 1404 2116 2181 2598
Size: 296 40 450 336 56 712 65 417
Cuts at: 2598 226
Size: 3681
Fragments arranged by size:
3681 712 450 417 336 296 65 56
40
Bpu10I CC'TnA_GC
Cuts at: 2909 2909
Size: 6053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3229 4108 4822 5120 3229
Size: 879 714 298 4162
Fragments arranged by size:
4162 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 988 1246 5757 988
Size: 258 4511 1284
Fragments arranged by size:
4511 1284 258
BsaAI yAC'GTr
Cuts at: 1224 3406 1224
Size: 2182 3871
BsaBI GATnn'nnATC
Cuts at: 6 3567 5604 6
Size: 3561 2037 455
Fragments arranged by size:
3561 2037 455
BsaHI Gr'CG_yC
Cuts at: 341 1259 3484 3732 4434 5930 341
Size: 918 2225 248 702 1496 464
Fragments arranged by size:
2225 1496 918 702 464 248
BsaWI w'CCGG_w
Cuts at: 489 665 3242 3763 4441 4852 4999 5273 489
Size: 176 2577 521 678 411 147 274 1269
Fragments arranged by size:
2577 1269 678 521 411 274 176 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1402 1432 2269 2299 1402
Size: 30 837 30 5156
Fragments arranged by size:
5156 837 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 259 764 2119 2290 205
Size: 54 505 1355 171 3968
Fragments arranged by size:
3968 1355 505 171 54
BseYI C'CCAG_C
Cuts at: 416 1301 2252 3478 4236 4901 5309 416
Size: 885 951 1226 758 665 408 1160
Fragments arranged by size:
1226 1160 951 885 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 1915 2189 2306 2734 2987 1825
Size: 90 274 117 428 253 4891
Fragments arranged by size:
4891 428 274 253 117 90
BsiEI CG_ry'CG
Cuts at: 104 296 395 3595 3641 4871 104
Size: 192 99 3200 46 1230 1286
Fragments arranged by size:
3200 1286 1230 192 99 46
BsiHKAI G_wGCw'C
Cuts at: 236 508 748 1439 2023 3067 3845 4035 4895
Size: 272 240 691 584 1044 778 190 860
Cuts at: 4895 236
Size: 1394
Fragments arranged by size:
1394 1044 860 778 691 584 272 240
190
BsmBI CGTCTCn'nnnn_
Cuts at: 169 169
Size: 6053
Bsp1286I G_dGCh'C
Cuts at: 236 417 508 748 1030 1439 1984 2023 2052
Size: 181 91 240 282 409 545 39 29
Cuts at: 2052 3067 3678 3771 3845 4035 4097 4895 236
Size: 1015 611 93 74 190 62 798 1394
Fragments arranged by size:
1394 1015 798 611 545 409 282 240
190 181 93 91 74 62 39 29
BspEI T'CCGG_A
Cuts at: 489 489
Size: 6053
BspHI T'CATG_A
Cuts at: 4485 4485
Size: 6053
BspMI ACCTGCnnnn'nnnn_
Cuts at: 557 633 1085 1220 1562 3103 3317 3619 4000
Size: 76 452 135 342 1541 214 302 381
Cuts at: 4000 557
Size: 2610
Fragments arranged by size:
2610 1541 452 381 342 302 214 135
76
BsrBI CCG'CTC
Cuts at: 50 187 1338 4345 4399 5883 6048 50
Size: 137 1151 3007 54 1484 165 55
Fragments arranged by size:
3007 1484 1151 165 137 55 54
BsrDI GCAATG_nn'
Cuts at: 3965 3965
Size: 6053
BsrFI r'CCGG_y
Cuts at: 385 4051 4232 385
Size: 3666 181 2206
Fragments arranged by size:
3666 2206 181
BsrGI T'GTAC_A
Cuts at: 1647 2340 1647
Size: 693 5360
BssSI C'ACGA_G
Cuts at: 4324 5032 4324
Size: 708 5345
BstAPI GCAn_nnn'nTGC
Cuts at: 1812 3430 1812
Size: 1618 4435
BstBI TT'CG_AA
Cuts at: 4414 4414
Size: 6053
BstEII G'GTnAC_C
Cuts at: 2941 2941
Size: 6053
BstXI CCAn_nnnn'nTGG
Cuts at: 853 853
Size: 6053
BstYI r'GATC_y
Cuts at: 1 147 194 497 954 1169 2309 3078 3545
Size: 146 47 303 457 215 1140 769 467
Cuts at: 3545 3568 3903 4149 4455 4467 4553 5605 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1140 1052 769 467 457 449 335 306
303 246 215 146 86 47 23 12
BtgI C'CryG_G
Cuts at: 107 116 198 326 409 846 1829 107
Size: 9 82 128 83 437 983 4331
Fragments arranged by size:
4331 983 437 128 83 82 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2689 4003 4184 4347 2689
Size: 1314 181 163 4395
Fragments arranged by size:
4395 1314 181 163
BtsI GCAGTG_nn'
Cuts at: 1732 2002 2172 2301 3296 1732
Size: 270 170 129 995 4489
Fragments arranged by size:
4489 995 270 170 129
DraI TTT'AAA
Cuts at: 3052 3052
Size: 6053
DraIII CAC_nnn'GTG
Cuts at: 1851 1851
Size: 6053
DrdI GACnn_nn'nnGTC
Cuts at: 3759 5103 3759
Size: 1344 4709
EaeI y'GGCC_r
Cuts at: 110 293 306 392 403 731 803 893 1949
Size: 183 13 86 11 328 72 90 1056
Cuts at: 1949 2237 2595 3638 3812 4203 4230 5575 110
Size: 288 358 1043 174 391 27 1345 588
Fragments arranged by size:
1345 1056 1043 588 391 358 328 288
183 174 90 86 72 27 13 11
EagI C'GGCC_G
Cuts at: 293 392 3638 293
Size: 99 3246 2708
Fragments arranged by size:
3246 2708 99
EarI CTCTTCn'nnn_
Cuts at: 758 985 1040 1222 1531 1880 2584 4076 4286
Size: 227 55 182 309 349 704 1492 210
Cuts at: 4286 758
Size: 2525
Fragments arranged by size:
2525 1492 704 349 309 227 210 182
55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 367 4261 5003 5149 5863 367
Size: 3894 742 146 714 557
Fragments arranged by size:
3894 742 714 557 146
EcoICRI GAG'CTC
Cuts at: 506 1437 2021 3065 506
Size: 931 584 1044 3494
Fragments arranged by size:
3494 1044 931 584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 226 522 562 925 1012 1348 1404 1702
Size: 69 296 40 363 87 336 56 298
Cuts at: 1702 2011 2116 2181 2598 3878 4310 4663 5325
Size: 309 105 65 417 1280 432 353 662
Cuts at: 5325 157
Size: 885
Fragments arranged by size:
1280 885 662 432 417 363 353 336
309 298 296 105 87 69 65 56
40
EcoNI CCTnn'n_nnAGG
Cuts at: 286 379 286
Size: 93 5960
EcoO109I rG'GnC_Cy
Cuts at: 738 3192 738
Size: 2454 3599
EcoRI G'AATT_C
Cuts at: 3057 3057
Size: 6053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 495 1356 1455 4072 495
Size: 861 99 2617 2476
Fragments arranged by size:
2617 2476 861 99
FspI TGC'GCA
Cuts at: 3834 3834
Size: 6053
HaeII r_GCGC'y
Cuts at: 213 2527 3487 3735 4965 5736 213
Size: 2314 960 248 1230 771 530
Fragments arranged by size:
2314 1230 960 771 530 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1402 1434 1714 1746 2212 2244 2269 2301 2853
Size: 32 280 32 466 32 25 32 552
Cuts at: 2853 2885 2963 2983 2995 3015 3895 3925 3927
Size: 32 78 20 12 20 880 30 2
Cuts at: 3927 3957 1402
Size: 30 3498
Fragments arranged by size:
3498 880 552 466 280 78 32 32
32 32 32 30 30 25 20 20
12 2
HincII GTy'rAC
Cuts at: 671 3092 671
Size: 2421 3632
KasI G'GCGC_C
Cuts at: 3483 3731 3483
Size: 248 5805
KpnI G_GTAC'C
Cuts at: 276 3073 276
Size: 2797 3256
MluI A'CGCG_T
Cuts at: 5486 5486
Size: 6053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2337 2364 4812 4996 2337
Size: 27 2448 184 3394
Fragments arranged by size:
3394 2448 184 27
MscI TGG'CCA
Cuts at: 112 805 895 1951 2239 3814 112
Size: 693 90 1056 288 1575 2351
Fragments arranged by size:
2351 1575 1056 693 288 90
MslI CAynn'nnrTG
Cuts at: 848 1222 4042 4169 848
Size: 374 2820 127 2732
Fragments arranged by size:
2820 2732 374 127
MspA1I CmG'CkG
Cuts at: 118 200 320 411 582 1064 1301 2252 2532
Size: 82 120 91 171 482 237 951 280
Cuts at: 2532 2938 3164 3478 3838 4622 4867 118
Size: 406 226 314 360 784 245 1304
Fragments arranged by size:
1304 951 784 482 406 360 314 280
245 237 226 171 120 91 82
NaeI GCC'GGC
Cuts at: 387 4234 387
Size: 3847 2206
NarI GG'CG_CC
Cuts at: 3484 3732 3484
Size: 248 5805
NcoI C'CATG_G
Cuts at: 107 846 107
Size: 739 5314
NgoMIV G'CCGG_C
Cuts at: 385 4232 385
Size: 3847 2206
NsiI A_TGCA'T
Cuts at: 1840 1840
Size: 6053
NspI r_CATG'y
Cuts at: 1179 1521 1838 3106 3606 5209 1179
Size: 342 317 1268 500 1603 2023
Fragments arranged by size:
2023 1603 1268 500 342 317
PasI CC'CwG_GG
Cuts at: 1968 1968
Size: 6053
PciI A'CATG_T
Cuts at: 1175 1517 5205 1175
Size: 342 3688 2023
Fragments arranged by size:
3688 2023 342
PflMI CCAn_nnn'nTGG
Cuts at: 729 847 1968 2252 729
Size: 118 1121 284 4530
Fragments arranged by size:
4530 1121 284 118
PfoI T'CCnGG_A
Cuts at: 1253 1253
Size: 6053
PmeI GTTT'AAAC
Cuts at: 3052 3052
Size: 6053
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 6021
PspOMI G'GGCC_C
Cuts at: 413 413
Size: 6053
PstI C_TGCA'G
Cuts at: 1043 1063 1163 2531 3100 3785 1043
Size: 20 100 1368 569 685 3311
Fragments arranged by size:
3311 1368 685 569 100 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6053
PvuII CAG'CTG
Cuts at: 582 1064 1301 2252 2532 2938 3478 3838 582
Size: 482 237 951 280 406 540 360 2797
Fragments arranged by size:
2797 951 540 482 406 360 280 237
RsrII CG'GwC_CG
Cuts at: 4248 4248
Size: 6053
SacI G_AGCT'C
Cuts at: 508 1439 2023 3067 508
Size: 931 584 1044 3494
Fragments arranged by size:
3494 1044 931 584
SacII CC_GC'GG
Cuts at: 119 201 412 119
Size: 82 211 5760
Fragments arranged by size:
5760 211 82
SalI G'TCGA_C
Cuts at: 3090 3090
Size: 6053
SapI GCTCTTCn'nnn_
Cuts at: 985 1222 1531 4076 4286 985
Size: 237 309 2545 210 2752
Fragments arranged by size:
2752 2545 309 237 210
SbfI CC_TGCA'GG
Cuts at: 1043 3100 1043
Size: 2057 3996
ScaI AGT'ACT
Cuts at: 364 2126 5570 364
Size: 1762 3444 847
Fragments arranged by size:
3444 1762 847
SexAI A'CCwGG_T
Cuts at: 2153 2153
Size: 6053
SfcI C'TryA_G
Cuts at: 33 450 1039 1059 1159 1873 2527 3096 3781
Size: 417 589 20 100 714 654 569 685
Cuts at: 3781 4749 4940 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 714 685 654 589 569 417
191 100 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 391 391
Size: 6053
SfoI GGC'GCC
Cuts at: 3485 3733 3485
Size: 248 5805
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6053
SmaI CCC'GGG
Cuts at: 3075 3075
Size: 6053
SmlI C'TyrA_G
Cuts at: 3208 4123 4837 5099 3208
Size: 915 714 262 4162
Fragments arranged by size:
4162 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6053
SphI G_CATG'C
Cuts at: 1838 3106 3606 1838
Size: 1268 500 4285
Fragments arranged by size:
4285 1268 500
StyI C'CwwG_G
Cuts at: 107 708 846 1430 1739 2573 3187 3541 107
Size: 601 138 584 309 834 614 354 2619
Fragments arranged by size:
2619 834 614 601 584 354 309 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1394 2215 2527 3636 3769 4228 4437 1394
Size: 821 312 1109 133 459 209 3010
Fragments arranged by size:
3010 1109 821 459 312 209 133
TatI w'GTAC_w
Cuts at: 362 1511 1647 2124 2340 5568 362
Size: 1149 136 477 216 3228 847
Fragments arranged by size:
3228 1149 847 477 216 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1249 1281 5570 5602 1249
Size: 32 4289 32 1700
Fragments arranged by size:
4289 1700 32 32
Tth111I GACn'n_nGTC
Cuts at: 1824 3850 1824
Size: 2026 4027
XbaI T'CTAG_A
Cuts at: 2214 3084 2214
Size: 870 5183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 844 1437 3548 844
Size: 593 2111 3349
Fragments arranged by size:
3349 2111 593
XmaI C'CCGG_G
Cuts at: 3073 3073
Size: 6053
XmnI GAAnn'nnTTC
Cuts at: 2087 2087
Size: 6053
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI AscI AvrII BaeI
BbvCI BcgI BmtI BsiWI BsmI BssHII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI