Restriction map for
pF1KA1375
(Circular) (Six-base) MAPSORT of: pF1KA1375.seq Check: 3160 from: 1 to: 5717
pF1KA1375 5717 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2289 2289
Size: 5717
AccI GT'mk_AC
Cuts at: 2755 2755
Size: 5717
Acc65I G'GTAC_C
Cuts at: 2733 2733
Size: 5717
AclI AA'CG_TT
Cuts at: 1530 5557 1530
Size: 4027 1690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1575 3542 3974 4327 4989 1575
Size: 1967 432 353 662 2303
Fragments arranged by size:
2303 1967 662 432 353
AflII C'TTAA_G
Cuts at: 618 2049 618
Size: 1431 4286
AflIII A'CryG_T
Cuts at: 3069 4869 5150 3069
Size: 1800 281 3636
Fragments arranged by size:
3636 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 668 668
Size: 5717
AleI CACnn'nnGTG
Cuts at: 1117 1666 1117
Size: 549 5168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 887 887
Size: 5717
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 335 367 335
Size: 32 5685
AlwNI CAG_nnn'CTG
Cuts at: 525 655 1690 3022 4460 525
Size: 130 1035 1332 1438 1782
Fragments arranged by size:
1782 1438 1332 1035 130
ApaLI G'TGCA_C
Cuts at: 4555 4555
Size: 5717
ApoI r'AATT_y
Cuts at: 349 913 1156 1519 1836 2011 2070 2721 5070
Size: 564 243 363 317 175 59 651 2349
Cuts at: 5070 5326 349
Size: 256 740
Fragments arranged by size:
2349 740 651 564 363 317 256 243
175 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 5717
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5717
AvaI C'yCGr_G
Cuts at: 301 2646 2737 301
Size: 2345 91 3281
Fragments arranged by size:
3281 2345 91
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2269 2302 2599 2632 2269
Size: 33 297 33 5354
Fragments arranged by size:
5354 297 33 33
BamHI G'GATC_C
Cuts at: 2742 2742
Size: 5717
BanI G'GyrC_C
Cuts at: 580 2733 3147 3395 3430 580
Size: 2153 414 248 35 2867
Fragments arranged by size:
2867 2153 414 248 35
BanII G_rGCy'C
Cuts at: 245 2412 2731 3761 245
Size: 2167 319 1030 2201
Fragments arranged by size:
2201 2167 1030 319
BbeI G_GCGC'C
Cuts at: 3151 3399 3151
Size: 248 5469
BbsI GAAGACnn'nnnn_
Cuts at: 2209 2209
Size: 5717
BbvCI CC'TCA_GC
Cuts at: 2678 2678
Size: 5717
BciVI GTATCCnnnnn_n'
Cuts at: 3615 4671 5717 3615
Size: 1056 1046 3615
Fragments arranged by size:
3615 1056 1046
BclI T'GATC_A
Cuts at: 324 1407 3237 324
Size: 1083 1830 2804
Fragments arranged by size:
2804 1830 1083
BglII A'GATC_T
Cuts at: 371 1089 1372 3232 5269 371
Size: 718 283 1860 2037 819
Fragments arranged by size:
2037 1860 819 718 283
BlpI GC'TnA_GC
Cuts at: 244 2481 2829 244
Size: 2237 348 3132
Fragments arranged by size:
3132 2237 348
Bme1580I G_kGCm'C
Cuts at: 1029 3342 3435 4559 1029
Size: 2313 93 1124 2187
Fragments arranged by size:
2313 2187 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 213 2929 3010 3345 5489 213
Size: 2716 81 335 2144 441
Fragments arranged by size:
2716 2144 441 335 81
BmtI G_CTAG'C
Cuts at: 503 1297 503
Size: 794 4923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 4084 2236
Size: 1848 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 1355 1834 2315 2417 632
Size: 723 479 481 102 3932
Fragments arranged by size:
3932 723 481 479 102
Bpu10I CC'TnA_GC
Cuts at: 453 495 1691 2678 453
Size: 42 1196 987 3492
Fragments arranged by size:
3492 1196 987 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 1859 2893 3772 4486 4784 677
Size: 1182 1034 879 714 298 1610
Fragments arranged by size:
1610 1182 1034 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2004 5421 2004
Size: 3417 2300
BsaAI yAC'GTr
Cuts at: 3070 3070
Size: 5717
BsaBI GATnn'nnATC
Cuts at: 6 1094 3231 5268 6
Size: 1088 2137 2037 455
Fragments arranged by size:
2137 2037 1088 455
BsaHI Gr'CG_yC
Cuts at: 3148 3396 4098 5594 3148
Size: 248 702 1496 3271
Fragments arranged by size:
3271 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2906 3427 4105 4516 4663 4937 2906
Size: 521 678 411 147 274 3686
Fragments arranged by size:
3686 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 416 446 1328 1358 416
Size: 30 882 30 4775
Fragments arranged by size:
4775 882 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 2312 2477 2480 253
Size: 2059 165 3 3490
Fragments arranged by size:
3490 2059 165 3
BseYI C'CCAG_C
Cuts at: 2363 2370 2443 2654 3142 3900 4565 4973 2363
Size: 7 73 211 488 758 665 408 3107
Fragments arranged by size:
3107 758 665 488 408 211 73 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 509 1636 148
Size: 361 1127 4229
Fragments arranged by size:
4229 1127 361
BsiEI CG_ry'CG
Cuts at: 104 273 635 3259 3305 4535 104
Size: 169 362 2624 46 1230 1286
Fragments arranged by size:
2624 1286 1230 362 169 46
BsiHKAI G_wGCw'C
Cuts at: 245 2265 2412 2731 3509 3699 4559 245
Size: 2020 147 319 778 190 860 1403
Fragments arranged by size:
2020 1403 860 778 319 190 147
BsmI GAATG_Cn'
Cuts at: 1704 1704
Size: 5717
BsmBI CGTCTCn'nnnn_
Cuts at: 298 595 298
Size: 297 5420
Bsp1286I G_dGCh'C
Cuts at: 245 1029 2265 2412 2731 3342 3435 3509 3699
Size: 784 1236 147 319 611 93 74 190
Cuts at: 3699 3761 4559 245
Size: 62 798 1403
Fragments arranged by size:
1403 1236 798 784 611 319 190 147
93 74 62
BspHI T'CATG_A
Cuts at: 1065 4149 1065
Size: 3084 2633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2289 2700 2767 2981 3283 3664 2289
Size: 411 67 214 302 381 4342
Fragments arranged by size:
4342 411 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 4009 4063 5547 5712 50
Size: 3959 54 1484 165 55
Fragments arranged by size:
3959 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 3629 2243
Size: 1386 4331
BsrFI r'CCGG_y
Cuts at: 3715 3896 3715
Size: 181 5536
BssHII G'CGCG_C
Cuts at: 296 296
Size: 5717
BssSI C'ACGA_G
Cuts at: 289 3988 4696 289
Size: 3699 708 1310
Fragments arranged by size:
3699 1310 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3094 3094
Size: 5717
BstBI TT'CG_AA
Cuts at: 4078 4078
Size: 5717
BstXI CCAn_nnnn'nTGG
Cuts at: 168 1278 2562 2629 168
Size: 1110 1284 67 3256
Fragments arranged by size:
3256 1284 1110 67
BstYI r'GATC_y
Cuts at: 1 371 1089 1095 1372 2742 3209 3232 3567
Size: 370 718 6 277 1370 467 23 335
Cuts at: 3567 3813 4119 4131 4217 5269 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1370 1052 718 467 449 370 335 306
277 246 86 23 12 6
BtgI C'CryG_G
Cuts at: 107 635 2561 107
Size: 528 1926 3263
Fragments arranged by size:
3263 1926 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3667 3848 4011 3667
Size: 181 163 5373
Fragments arranged by size:
5373 181 163
BtsI GCAGTG_nn'
Cuts at: 1749 2187 2960 1749
Size: 438 773 4506
Fragments arranged by size:
4506 773 438
DraI TTT'AAA
Cuts at: 710 2716 710
Size: 2006 3711
DrdI GACnn_nn'nnGTC
Cuts at: 3423 4767 3423
Size: 1344 4373
EaeI y'GGCC_r
Cuts at: 158 2586 3302 3476 3867 3894 5239 158
Size: 2428 716 174 391 27 1345 636
Fragments arranged by size:
2428 1345 716 636 391 174 27
EagI C'GGCC_G
Cuts at: 3302 3302
Size: 5717
EarI CTCTTCn'nnn_
Cuts at: 203 3740 3950 203
Size: 3537 210 1970
Fragments arranged by size:
3537 1970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 250 3925 4667 4813 5527 250
Size: 3675 742 146 714 440
Fragments arranged by size:
3675 742 714 440 146
EcoICRI GAG'CTC
Cuts at: 243 2410 2729 243
Size: 2167 319 3231
Fragments arranged by size:
3231 2167 319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 1355 1575 1834 2315 2417 3542 3974 4327
Size: 723 220 259 481 102 1125 432 353
Cuts at: 4327 4989 632
Size: 662 1360
Fragments arranged by size:
1360 1125 723 662 481 432 353 259
220 102
EcoO109I rG'GnC_Cy
Cuts at: 662 2650 2856 662
Size: 1988 206 3523
Fragments arranged by size:
3523 1988 206
EcoRI G'AATT_C
Cuts at: 2721 2721
Size: 5717
EcoRV GAT'ATC
Cuts at: 2533 2533
Size: 5717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 433 1132 1918 3736 433
Size: 699 786 1818 2414
Fragments arranged by size:
2414 1818 786 699
FspI TGC'GCA
Cuts at: 3498 3498
Size: 5717
HaeII r_GCGC'y
Cuts at: 2338 3151 3399 4629 5400 2338
Size: 813 248 1230 771 2655
Fragments arranged by size:
2655 1230 813 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 533 565 654 686 1392 1424 1813 1845 3559
Size: 32 89 32 706 32 389 32 1714
Cuts at: 3559 3589 3591 3621 533
Size: 30 2 30 2629
Fragments arranged by size:
2629 1714 706 389 89 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1242 2147 2756 1242
Size: 905 609 4203
Fragments arranged by size:
4203 905 609
HindIII A'AGCT_T
Cuts at: 450 450
Size: 5717
HpaI GTT'AAC
Cuts at: 1242 1242
Size: 5717
KasI G'GCGC_C
Cuts at: 3147 3395 3147
Size: 248 5469
KpnI G_GTAC'C
Cuts at: 2737 2737
Size: 5717
MfeI C'AATT_G
Cuts at: 483 1250 1319 483
Size: 767 69 4881
Fragments arranged by size:
4881 767 69
MluI A'CGCG_T
Cuts at: 5150 5150
Size: 5717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1460 2350 4476 4660 1460
Size: 890 2126 184 2517
Fragments arranged by size:
2517 2126 890 184
MscI TGG'CCA
Cuts at: 160 2588 3478 160
Size: 2428 890 2399
Fragments arranged by size:
2428 2399 890
MslI CAynn'nnrTG
Cuts at: 124 1117 1666 2560 3706 3833 124
Size: 993 549 894 1146 127 2008
Fragments arranged by size:
2008 1146 993 894 549 127
MspA1I CmG'CkG
Cuts at: 132 168 279 2828 3142 3502 4286 4531 132
Size: 36 111 2549 314 360 784 245 1318
Fragments arranged by size:
2549 1318 784 360 314 245 111 36
NaeI GCC'GGC
Cuts at: 3898 3898
Size: 5717
NarI GG'CG_CC
Cuts at: 3148 3396 3148
Size: 248 5469
NcoI C'CATG_G
Cuts at: 107 2561 107
Size: 2454 3263
NdeI CA'TA_TG
Cuts at: 2085 2085
Size: 5717
NgoMIV G'CCGG_C
Cuts at: 3896 3896
Size: 5717
NheI G'CTAG_C
Cuts at: 499 1293 499
Size: 794 4923
NspI r_CATG'y
Cuts at: 946 2314 2770 3270 4873 946
Size: 1368 456 500 1603 1790
Fragments arranged by size:
1790 1603 1368 500 456
PciI A'CATG_T
Cuts at: 4869 4869
Size: 5717
PfoI T'CCnGG_A
Cuts at: 2526 2628 2526
Size: 102 5615
PmeI GTTT'AAAC
Cuts at: 2716 2716
Size: 5717
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 4163
Size: 32 5685
PpuMI rG'GwC_Cy
Cuts at: 662 2650 662
Size: 1988 3729
PstI C_TGCA'G
Cuts at: 771 1038 2764 3449 771
Size: 267 1726 685 3039
Fragments arranged by size:
3039 1726 685 267
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5717
PvuII CAG'CTG
Cuts at: 132 3142 3502 132
Size: 3010 360 2347
Fragments arranged by size:
3010 2347 360
RsrII CG'GwC_CG
Cuts at: 3912 3912
Size: 5717
SacI G_AGCT'C
Cuts at: 245 2412 2731 245
Size: 2167 319 3231
Fragments arranged by size:
3231 2167 319
SalI G'TCGA_C
Cuts at: 2754 2754
Size: 5717
SanDI GG'GwC_CC
Cuts at: 662 2650 662
Size: 1988 3729
SapI GCTCTTCn'nnn_
Cuts at: 203 3740 3950 203
Size: 3537 210 1970
Fragments arranged by size:
3537 1970 210
SbfI CC_TGCA'GG
Cuts at: 2764 2764
Size: 5717
ScaI AGT'ACT
Cuts at: 223 5234 223
Size: 5011 706
SfcI C'TryA_G
Cuts at: 33 767 1034 2760 3445 4413 4604 33
Size: 734 267 1726 685 968 191 1146
Fragments arranged by size:
1726 1146 968 734 685 267 191
SfoI GGC'GCC
Cuts at: 3149 3397 3149
Size: 248 5469
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5717
SmaI CCC'GGG
Cuts at: 2648 2739 2648
Size: 91 5626
SmlI C'TyrA_G
Cuts at: 301 618 692 1874 2049 2872 3787 4501 4763
Size: 317 74 1182 175 823 915 714 262
Cuts at: 4763 301
Size: 1255
Fragments arranged by size:
1255 1182 915 823 714 317 262 175
74
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5717
SphI G_CATG'C
Cuts at: 946 2770 3270 946
Size: 1824 500 3393
Fragments arranged by size:
3393 1824 500
SspI AAT'ATT
Cuts at: 1992 1992
Size: 5717
StuI AGG'CCT
Cuts at: 2379 2379
Size: 5717
StyI C'CwwG_G
Cuts at: 107 821 2561 2851 3205 107
Size: 714 1740 290 354 2619
Fragments arranged by size:
2619 1740 714 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 259 3300 3433 3892 4101 259
Size: 3041 133 459 209 1875
Fragments arranged by size:
3041 1875 459 209 133
TatI w'GTAC_w
Cuts at: 221 1194 5232 221
Size: 973 4038 706
Fragments arranged by size:
4038 973 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5234 5266 5234
Size: 32 5685
Tth111I GACn'n_nGTC
Cuts at: 3514 3514
Size: 5717
XbaI T'CTAG_A
Cuts at: 1092 2748 1092
Size: 1656 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 574 3212 574
Size: 2638 3079
XhoI C'TCGA_G
Cuts at: 301 301
Size: 5717
XmaI C'CCGG_G
Cuts at: 2646 2737 2646
Size: 91 5626
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AgeI ApaI AscI AvrII BcgI BfrBI
BglI BmgBI BsiWI BspEI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XmnI ZraI