Restriction map for
pF1KA1373
(Circular) (Six-base) MAPSORT of: pF1KA1373.seq Check: 2844 from: 1 to: 4496
pF1KA1373 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1257 2321 2753 3500 3768 457
Size: 800 1064 432 747 268 1185
Fragments arranged by size:
1185 1064 800 747 432 268
AfeI AGC'GCT
Cuts at: 735 735
Size: 4496
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 409 639 1801 3368 409
Size: 230 1162 1567 1537
Fragments arranged by size:
1567 1537 1162 230
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 129 315 346 545 885 940 1021 1369 1500
Size: 186 31 199 340 55 81 348 131
Cuts at: 1500 3849 4105 129
Size: 2349 256 520
Fragments arranged by size:
2349 520 348 340 256 199 186 131
81 55 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 677 1516 677
Size: 839 3657
BamHI G'GATC_C
Cuts at: 1142 1521 1142
Size: 379 4117
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 1349 1415 1510 2540 1349
Size: 66 95 1030 3305
Fragments arranged by size:
3305 1030 95 66
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 245 245
Size: 4496
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglII A'GATC_T
Cuts at: 927 2011 4048 927
Size: 1084 2037 1375
Fragments arranged by size:
2037 1375 1084
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 204 2121 2214 3270 204
Size: 1917 93 1056 1430
Fragments arranged by size:
1917 1430 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BmtI G_CTAG'C
Cuts at: 827 827
Size: 4496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 290
Size: 4496
Bpu10I CC'TnA_GC
Cuts at: 178 205 178
Size: 27 4469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1318 1672 2551 3043 3341 643
Size: 675 354 879 492 298 1798
Fragments arranged by size:
1798 879 675 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 292 1927 2175 2877 4373 292
Size: 1635 248 702 1496 415
Fragments arranged by size:
1635 1496 702 415 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1109 1139 1109
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 824 1117 824
Size: 293 4203
BseYI C'CCAG_C
Cuts at: 1921 2679 3256 3752 1921
Size: 758 577 496 2665
Fragments arranged by size:
2665 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 422
Size: 4496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1510 2288 2478 3270 1510
Size: 778 190 792 2736
Fragments arranged by size:
2736 792 778 190
BsmI GAATG_Cn'
Cuts at: 448 448
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 204 1349 1415 1510 2121 2214 2288 2478 2540
Size: 1145 66 95 611 93 74 190 62
Cuts at: 2540 3270 204
Size: 730 1430
Fragments arranged by size:
1430 1145 730 611 190 95 93 74
66 62
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 403 1229 2408 403
Size: 826 1179 2491
Fragments arranged by size:
2491 1179 826
BsrFI r'CCGG_y
Cuts at: 2494 2675 2884 2494
Size: 181 209 4106
Fragments arranged by size:
4106 209 181
BssSI C'ACGA_G
Cuts at: 2767 3125 2767
Size: 358 4138
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 647 2857 647
Size: 2210 2286
BstYI r'GATC_y
Cuts at: 1 927 1142 1457 1521 1988 2011 2346 2592
Size: 926 215 315 64 467 23 335 246
Cuts at: 2592 3604 3690 3702 4048 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 926 467 449 346 335 315 246
215 86 64 23 12
Bsu36I CC'TnA_GG
Cuts at: 705 705
Size: 4496
BtgI C'CryG_G
Cuts at: 107 107
Size: 4496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 921 2446 2627 2790 921
Size: 1525 181 163 2627
Fragments arranged by size:
2627 1525 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1739 1394
Size: 345 4151
DraI TTT'AAA
Cuts at: 485 1495 485
Size: 1010 3486
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 2081 2255 2646 2673 4018 2081
Size: 174 391 27 1345 2559
Fragments arranged by size:
2559 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 182 2519 2729 182
Size: 2337 210 1949
Fragments arranged by size:
2337 1949 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 457 1257 2321 2753 3500 3768 290
Size: 167 800 1064 432 747 268 1018
Fragments arranged by size:
1064 1018 800 747 432 268 167
EcoO109I rG'GnC_Cy
Cuts at: 848 1635 848
Size: 787 3709
EcoRI G'AATT_C
Cuts at: 129 1500 129
Size: 1371 3125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 737 1930 2178 3200 4179 737
Size: 1193 248 1022 979 1054
Fragments arranged by size:
1193 1054 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1433 1465 2338 2368 2370 2400 1433
Size: 32 873 30 2 30 3529
Fragments arranged by size:
3529 873 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
HindIII A'AGCT_T
Cuts at: 211 370 211
Size: 159 4337
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 167 2485 2612 167
Size: 2318 127 2051
Fragments arranged by size:
2318 2051 127
MspA1I CmG'CkG
Cuts at: 862 1607 1921 2281 3294 3539 862
Size: 745 314 360 1013 245 1819
Fragments arranged by size:
1819 1013 745 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NheI G'CTAG_C
Cuts at: 823 823
Size: 4496
NspI r_CATG'y
Cuts at: 1296 1549 2049 2956 1296
Size: 253 500 907 2836
Fragments arranged by size:
2836 907 500 253
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PsiI TTA'TAA
Cuts at: 341 352 341
Size: 11 4485
PstI C_TGCA'G
Cuts at: 1543 2228 1543
Size: 685 3811
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 862 1921 2281 862
Size: 1059 360 3077
Fragments arranged by size:
3077 1059 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 182 2519 2729 182
Size: 2337 210 1949
Fragments arranged by size:
2337 1949 210
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 1539 2224 3217 3408 33
Size: 1506 685 993 191 1121
Fragments arranged by size:
1506 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 658 1297 1651 2566 3058 3320 658
Size: 639 354 915 492 262 1834
Fragments arranged by size:
1834 915 639 492 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1296 1549 2049 1296
Size: 253 500 3743
Fragments arranged by size:
3743 500 253
SspI AAT'ATT
Cuts at: 514 514
Size: 4496
StyI C'CwwG_G
Cuts at: 107 454 1340 1630 1984 107
Size: 347 886 290 354 2619
Fragments arranged by size:
2619 886 354 347 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2079 2212 2671 2880 2079
Size: 133 459 209 3695
Fragments arranged by size:
3695 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 371 371
Size: 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI