Restriction map for
pF1KA1370
(Circular) (Six-base) MAPSORT of: pF1KA1370.seq Check: 3278 from: 1 to: 6341
pF1KA1370 6341 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1290 1290
Size: 6341
AccI GT'mk_AC
Cuts at: 656 2419 3379 656
Size: 1763 960 3618
Fragments arranged by size:
3618 1763 960
Acc65I G'GTAC_C
Cuts at: 3357 3357
Size: 6341
AclI AA'CG_TT
Cuts at: 6181 6181
Size: 6341
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2110 2951 4166 4598 4951 5613 2110
Size: 841 1215 432 353 662 2838
Fragments arranged by size:
2838 1215 841 662 432 353
AflIII A'CryG_T
Cuts at: 1275 2245 2422 3693 5493 5774 1275
Size: 970 177 1271 1800 281 1842
Fragments arranged by size:
1842 1800 1271 970 281 177
AhdI GACnn_n'nnGTC
Cuts at: 2415 2415
Size: 6341
AlwNI CAG_nnn'CTG
Cuts at: 906 1018 3646 5084 906
Size: 112 2628 1438 2163
Fragments arranged by size:
2628 2163 1438 112
ApaI G_GGCC'C
Cuts at: 422 422
Size: 6341
ApaLI G'TGCA_C
Cuts at: 5179 5179
Size: 6341
ApoI r'AATT_y
Cuts at: 669 824 1439 1692 2110 2748 3345 5694 5950
Size: 155 615 253 418 638 597 2349 256
Cuts at: 5950 669
Size: 1060
Fragments arranged by size:
2349 1060 638 615 597 418 256 253
155
AseI AT'TA_AT
Cuts at: 20 20
Size: 6341
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6341
AvaI C'yCGr_G
Cuts at: 3361 3361
Size: 6341
AvrII C'CTAG_G
Cuts at: 2742 2742
Size: 6341
BamHI G'GATC_C
Cuts at: 3366 3366
Size: 6341
BanI G'GyrC_C
Cuts at: 933 3357 3771 4019 4054 933
Size: 2424 414 248 35 3220
Fragments arranged by size:
3220 2424 414 248 35
BanII G_rGCy'C
Cuts at: 422 3355 4385 422
Size: 2933 1030 2378
Fragments arranged by size:
2933 2378 1030
BbeI G_GCGC'C
Cuts at: 3775 4023 3775
Size: 248 6093
BbsI GAAGACnn'nnnn_
Cuts at: 544 1427 1691 544
Size: 883 264 5194
Fragments arranged by size:
5194 883 264
BciVI GTATCCnnnnn_n'
Cuts at: 4239 5295 6341 4239
Size: 1056 1046 4239
Fragments arranged by size:
4239 1056 1046
BclI T'GATC_A
Cuts at: 3861 3861
Size: 6341
BfrBI ATG'CAT
Cuts at: 469 1638 469
Size: 1169 5172
BglII A'GATC_T
Cuts at: 3209 3856 5893 3209
Size: 647 2037 3657
Fragments arranged by size:
3657 2037 647
BlpI GC'TnA_GC
Cuts at: 1577 3453 1577
Size: 1876 4465
Bme1580I G_kGCm'C
Cuts at: 422 3966 4059 5183 422
Size: 3544 93 1124 1580
Fragments arranged by size:
3544 1580 1124 93
BmgBI CAC'GTC
Cuts at: 785 785
Size: 6341
BmrI ACTGGGnnnn_n'
Cuts at: 262 300 3553 3634 3969 6113 262
Size: 38 3253 81 335 2144 490
Fragments arranged by size:
3253 2144 490 335 81 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 620 4708 620
Size: 4088 2253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 1528 1629 233
Size: 1295 101 4945
Fragments arranged by size:
4945 1295 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1286 3517 4396 5110 5408 1031
Size: 255 2231 879 714 298 1964
Fragments arranged by size:
2231 1964 879 714 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 174 6045 174
Size: 5871 470
BsaAI yAC'GTr
Cuts at: 1898 3694 1898
Size: 1796 4545
BsaBI GATnn'nnATC
Cuts at: 6 3855 5892 6
Size: 3849 2037 455
Fragments arranged by size:
3849 2037 455
BsaHI Gr'CG_yC
Cuts at: 1287 3772 4020 4722 6218 1287
Size: 2485 248 702 1496 1410
Fragments arranged by size:
2485 1496 1410 702 248
BsaWI w'CCGG_w
Cuts at: 3530 4051 4729 5140 5287 5561 3530
Size: 521 678 411 147 274 4310
Fragments arranged by size:
4310 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 1451 172
Size: 1279 5062
BseYI C'CCAG_C
Cuts at: 3766 4524 5189 5597 3766
Size: 758 665 408 4510
Fragments arranged by size:
4510 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 807 807
Size: 6341
BsiEI CG_ry'CG
Cuts at: 104 3883 3929 5159 104
Size: 3779 46 1230 1286
Fragments arranged by size:
3779 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1592 2718 3355 4133 4323 5183 1592
Size: 1126 637 778 190 860 2750
Fragments arranged by size:
2750 1126 860 778 637 190
BsiWI C'GTAC_G
Cuts at: 3228 3228
Size: 6341
BsmBI CGTCTCn'nnnn_
Cuts at: 3218 3218
Size: 6341
Bsp1286I G_dGCh'C
Cuts at: 422 1592 2718 3355 3966 4059 4133 4323 4385
Size: 1170 1126 637 611 93 74 190 62
Cuts at: 4385 5183 422
Size: 798 1580
Fragments arranged by size:
1580 1170 1126 798 637 611 190 93
74 62
BspHI T'CATG_A
Cuts at: 2688 4773 2688
Size: 2085 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3391 3605 3907 4288 3391
Size: 214 302 381 5444
Fragments arranged by size:
5444 381 302 214
BsrBI CCG'CTC
Cuts at: 50 560 1215 1551 4633 4687 6171 6336 50
Size: 510 655 336 3082 54 1484 165 55
Fragments arranged by size:
3082 1484 655 510 336 165 55 54
BsrDI GCAATG_nn'
Cuts at: 240 1226 2016 4253 240
Size: 986 790 2237 2328
Fragments arranged by size:
2328 2237 986 790
BsrFI r'CCGG_y
Cuts at: 4339 4520 4339
Size: 181 6160
BsrGI T'GTAC_A
Cuts at: 2062 2062
Size: 6341
BssSI C'ACGA_G
Cuts at: 4612 5320 4612
Size: 708 5633
BstAPI GCAn_nnn'nTGC
Cuts at: 1237 1573 2830 3718 1237
Size: 336 1257 888 3860
Fragments arranged by size:
3860 1257 888 336
BstBI TT'CG_AA
Cuts at: 4702 4702
Size: 6341
BstEII G'GTnAC_C
Cuts at: 517 517
Size: 6341
BstYI r'GATC_y
Cuts at: 1 488 2358 3209 3366 3833 3856 4191 4437
Size: 487 1870 851 157 467 23 335 246
Cuts at: 4437 4743 4755 4841 5893 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1870 1052 851 487 467 449 335 306
246 157 86 23 12
BtgI C'CryG_G
Cuts at: 929 1035 929
Size: 106 6235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 425 4291 4472 4635 425
Size: 3866 181 163 2131
Fragments arranged by size:
3866 2131 181 163
BtsI GCAGTG_nn'
Cuts at: 3584 3584
Size: 6341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 356 391 356
Size: 35 6306
DraI TTT'AAA
Cuts at: 1115 1812 3340 1115
Size: 697 1528 4116
Fragments arranged by size:
4116 1528 697
DrdI GACnn_nn'nnGTC
Cuts at: 4047 5391 4047
Size: 1344 4997
EaeI y'GGCC_r
Cuts at: 3926 4100 4491 4518 5863 3926
Size: 174 391 27 1345 4404
Fragments arranged by size:
4404 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3926 3926
Size: 6341
EarI CTCTTCn'nnn_
Cuts at: 527 2558 2861 3109 4364 4574 527
Size: 2031 303 248 1255 210 2294
Fragments arranged by size:
2294 2031 1255 303 248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4549 5291 5437 6151 4549
Size: 742 146 714 4739
Fragments arranged by size:
4739 742 714 146
EcoICRI GAG'CTC
Cuts at: 3353 3353
Size: 6341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 1528 1629 2110 2951 4166 4598 4951 5613
Size: 1295 101 481 841 1215 432 353 662
Cuts at: 5613 233
Size: 961
Fragments arranged by size:
1295 1215 961 841 662 481 432 353
101
EcoNI CCTnn'n_nnAGG
Cuts at: 1193 1529 2966 1193
Size: 336 1437 4568
Fragments arranged by size:
4568 1437 336
EcoO109I rG'GnC_Cy
Cuts at: 3480 3480
Size: 6341
EcoRI G'AATT_C
Cuts at: 824 3345 824
Size: 2521 3820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1490 2849 4360 1490
Size: 1359 1511 3471
Fragments arranged by size:
3471 1511 1359
FspI TGC'GCA
Cuts at: 4122 4122
Size: 6341
HaeII r_GCGC'y
Cuts at: 3775 4023 5253 6024 3775
Size: 248 1230 771 4092
Fragments arranged by size:
4092 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1072 1104 1705 1737 2401 2433 4183 4213 4215
Size: 32 601 32 664 32 1750 30 2
Cuts at: 4215 4245 1072
Size: 30 3168
Fragments arranged by size:
3168 1750 664 601 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3380 3380
Size: 6341
HindIII A'AGCT_T
Cuts at: 234 1376 2631 234
Size: 1142 1255 3944
Fragments arranged by size:
3944 1255 1142
KasI G'GCGC_C
Cuts at: 3771 4019 3771
Size: 248 6093
KpnI G_GTAC'C
Cuts at: 3361 3361
Size: 6341
MfeI C'AATT_G
Cuts at: 686 686
Size: 6341
MluI A'CGCG_T
Cuts at: 5774 5774
Size: 6341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1491 1624 2156 2386 3165 5100 5284 1491
Size: 133 532 230 779 1935 184 2548
Fragments arranged by size:
2548 1935 779 532 230 184 133
MscI TGG'CCA
Cuts at: 4102 4102
Size: 6341
MslI CAynn'nnrTG
Cuts at: 207 348 2474 3330 4330 4457 207
Size: 141 2126 856 1000 127 2091
Fragments arranged by size:
2126 2091 1000 856 141 127
MspA1I CmG'CkG
Cuts at: 988 1174 3452 3766 4126 4910 5155 988
Size: 186 2278 314 360 784 245 2174
Fragments arranged by size:
2278 2174 784 360 314 245 186
NaeI GCC'GGC
Cuts at: 4522 4522
Size: 6341
NarI GG'CG_CC
Cuts at: 3772 4020 3772
Size: 248 6093
NcoI C'CATG_G
Cuts at: 929 1035 929
Size: 106 6235
NdeI CA'TA_TG
Cuts at: 1502 1680 3279 1502
Size: 178 1599 4564
Fragments arranged by size:
4564 1599 178
NgoMIV G'CCGG_C
Cuts at: 4520 4520
Size: 6341
NsiI A_TGCA'T
Cuts at: 471 1640 471
Size: 1169 5172
NspI r_CATG'y
Cuts at: 697 955 1279 1638 2214 2249 2426 3394 3894
Size: 258 324 359 576 35 177 968 500
Cuts at: 3894 5497 697
Size: 1603 1541
Fragments arranged by size:
1603 1541 968 576 500 359 324 258
177 35
PciI A'CATG_T
Cuts at: 1275 2245 2422 5493 1275
Size: 970 177 3071 2123
Fragments arranged by size:
3071 2123 970 177
PflMI CCAn_nnn'nTGG
Cuts at: 1018 1018
Size: 6341
PfoI T'CCnGG_A
Cuts at: 1544 1544
Size: 6341
PmeI GTTT'AAAC
Cuts at: 3340 3340
Size: 6341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4787 4819 4787
Size: 32 6309
PspOMI G'GGCC_C
Cuts at: 418 418
Size: 6341
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 879 911 3146 3178 879
Size: 32 2235 32 4042
Fragments arranged by size:
4042 2235 32 32
PstI C_TGCA'G
Cuts at: 987 3388 4073 987
Size: 2401 685 3255
Fragments arranged by size:
3255 2401 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6341
PvuII CAG'CTG
Cuts at: 988 1174 3766 4126 988
Size: 186 2592 360 3203
Fragments arranged by size:
3203 2592 360 186
RsrII CG'GwC_CG
Cuts at: 4536 4536
Size: 6341
SacI G_AGCT'C
Cuts at: 3355 3355
Size: 6341
SalI G'TCGA_C
Cuts at: 3378 3378
Size: 6341
SapI GCTCTTCn'nnn_
Cuts at: 4364 4574 4364
Size: 210 6131
SbfI CC_TGCA'GG
Cuts at: 3388 3388
Size: 6341
ScaI AGT'ACT
Cuts at: 5858 5858
Size: 6341
SfcI C'TryA_G
Cuts at: 33 405 633 661 983 2877 3384 4069 5037
Size: 372 228 28 322 1894 507 685 968
Cuts at: 5037 5228 33
Size: 191 1146
Fragments arranged by size:
1894 1146 968 685 507 372 322 228
191 28
SfoI GGC'GCC
Cuts at: 3773 4021 3773
Size: 248 6093
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6341
SmaI CCC'GGG
Cuts at: 3363 3363
Size: 6341
SmlI C'TyrA_G
Cuts at: 1046 1301 3496 4411 5125 5387 1046
Size: 255 2195 915 714 262 2000
Fragments arranged by size:
2195 2000 915 714 262 255
SnaBI TAC'GTA
Cuts at: 1898 1898
Size: 6341
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6341
SphI G_CATG'C
Cuts at: 3394 3894 3394
Size: 500 5841
SspI AAT'ATT
Cuts at: 139 627 804 1056 2191 139
Size: 488 177 252 1135 4289
Fragments arranged by size:
4289 1135 488 252 177
StuI AGG'CCT
Cuts at: 2238 2238
Size: 6341
StyI C'CwwG_G
Cuts at: 929 1035 2504 2742 2937 3475 3829 929
Size: 106 1469 238 195 538 354 3441
Fragments arranged by size:
3441 1469 538 354 238 195 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 134 536 2905 3924 4057 4516 4725 134
Size: 402 2369 1019 133 459 209 1750
Fragments arranged by size:
2369 1750 1019 459 402 209 133
TatI w'GTAC_w
Cuts at: 2062 5856 2062
Size: 3794 2547
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5858 5890 5858
Size: 32 6309
Tth111I GACn'n_nGTC
Cuts at: 4138 4138
Size: 6341
XbaI T'CTAG_A
Cuts at: 2641 3244 3372 2641
Size: 603 128 5610
Fragments arranged by size:
5610 603 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3836 3836
Size: 6341
XmaI C'CCGG_G
Cuts at: 3361 3361
Size: 6341
XmnI GAAnn'nnTTC
Cuts at: 236 2632 3087 236
Size: 2396 455 3490
Fragments arranged by size:
3490 2396 455
ZraI GAC'GTC
Cuts at: 1288 1288
Size: 6341
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AlfI AloI AscI
BaeI BbvCI BcgI BglI BmtI Bpu10I BsaXI BsmI
BspEI BssHII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HpaI NheI NotI NruI PacI PasI
PmlI PpuMI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI