Restriction map for
pF1KA1364
(Circular) (Six-base) MAPSORT of: pF1KA1364.seq Check: 6408 from: 1 to: 5957
pF1KA1364 5957 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 187 187
Size: 5957
AatII G_ACGT'C
Cuts at: 2900 2900
Size: 5957
AccI GT'mk_AC
Cuts at: 2420 2995 2420
Size: 575 5382
Acc65I G'GTAC_C
Cuts at: 2832 2973 2832
Size: 141 5816
AclI AA'CG_TT
Cuts at: 5797 5797
Size: 5957
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1470 3782 4214 4567 5229 1470
Size: 2312 432 353 662 2198
Fragments arranged by size:
2312 2198 662 432 353
AflIII A'CryG_T
Cuts at: 3309 5109 5390 3309
Size: 1800 281 3876
Fragments arranged by size:
3876 1800 281
AgeI A'CCGG_T
Cuts at: 158 158
Size: 5957
AlwNI CAG_nnn'CTG
Cuts at: 939 1158 2295 2379 3262 4700 939
Size: 219 1137 84 883 1438 2196
Fragments arranged by size:
2196 1438 1137 883 219 84
ApaLI G'TGCA_C
Cuts at: 357 718 4795 357
Size: 361 4077 1519
Fragments arranged by size:
4077 1519 361
ApoI r'AATT_y
Cuts at: 652 751 814 849 925 1620 2302 2539 2961
Size: 99 63 35 76 695 682 237 422
Cuts at: 2961 5310 5566 652
Size: 2349 256 1043
Fragments arranged by size:
2349 1043 695 682 422 256 237 99
76 63 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 5957
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
AvaI C'yCGr_G
Cuts at: 1085 1343 2186 2977 1085
Size: 258 843 791 4065
Fragments arranged by size:
4065 843 791 258
AvrII C'CTAG_G
Cuts at: 624 624
Size: 5957
BamHI G'GATC_C
Cuts at: 2982 2982
Size: 5957
BanI G'GyrC_C
Cuts at: 529 1688 2650 2764 2832 2884 2930 2973 3387
Size: 1159 962 114 68 52 46 43 414
Cuts at: 3387 3635 3670 529
Size: 248 35 2816
Fragments arranged by size:
2816 1159 962 414 248 114 68 52
46 43 35
BanII G_rGCy'C
Cuts at: 212 1076 1408 2353 2741 2971 4001 212
Size: 864 332 945 388 230 1030 2168
Fragments arranged by size:
2168 1030 945 864 388 332 230
BbeI G_GCGC'C
Cuts at: 2654 2888 2934 3391 3639 2654
Size: 234 46 457 248 4972
Fragments arranged by size:
4972 457 248 234 46
BbvCI CC'TCA_GC
Cuts at: 2816 2816
Size: 5957
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2274 2308 2274
Size: 34 5923
BciVI GTATCCnnnnn_n'
Cuts at: 592 1529 3855 4911 5957 592
Size: 937 2326 1056 1046 592
Fragments arranged by size:
2326 1056 1046 937 592
BclI T'GATC_A
Cuts at: 761 3477 761
Size: 2716 3241
BglI GCCn_nnn'nGGC
Cuts at: 304 844 1129 1320 304
Size: 540 285 191 4941
Fragments arranged by size:
4941 540 285 191
BglII A'GATC_T
Cuts at: 1731 3472 5509 1731
Size: 1741 2037 2179
Fragments arranged by size:
2179 2037 1741
BlpI GC'TnA_GC
Cuts at: 1114 3069 1114
Size: 1955 4002
Bme1580I G_kGCm'C
Cuts at: 361 714 722 2767 3582 3675 4799 361
Size: 353 8 2045 815 93 1124 1519
Fragments arranged by size:
2045 1519 1124 815 353 93 8
BmgBI CAC'GTC
Cuts at: 642 642
Size: 5957
BmrI ACTGGGnnnn_n'
Cuts at: 439 3169 3250 3585 5729 439
Size: 2730 81 335 2144 667
Fragments arranged by size:
2730 2144 667 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4324 4324
Size: 5957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 2752 2806 581
Size: 2171 54 3732
Fragments arranged by size:
3732 2171 54
Bpu10I CC'TnA_GC
Cuts at: 2068 2816 2863 2068
Size: 748 47 5162
Fragments arranged by size:
5162 748 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 250 3133 4012 4726 5024 178
Size: 72 2883 879 714 298 1111
Fragments arranged by size:
2883 1111 879 714 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 121 406 1397 2589 5661 121
Size: 285 991 1192 3072 417
Fragments arranged by size:
3072 1192 991 417 285
BsaAI yAC'GTr
Cuts at: 2424 3310 2424
Size: 886 5071
BsaBI GATnn'nnATC
Cuts at: 6 3471 5508 6
Size: 3465 2037 455
Fragments arranged by size:
3465 2037 455
BsaHI Gr'CG_yC
Cuts at: 2651 2885 2897 2931 3388 3636 4338 5834 2651
Size: 234 12 34 457 248 702 1496 2774
Fragments arranged by size:
2774 1496 702 457 248 234 34 12
BsaWI w'CCGG_w
Cuts at: 158 2462 2835 3146 3667 4345 4756 4903 5177
Size: 2304 373 311 521 678 411 147 274
Cuts at: 5177 158
Size: 938
Fragments arranged by size:
2304 938 678 521 411 373 311 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 232
Size: 30 5927
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 2035 2163 2174 2185 2188 2213 2749 2854
Size: 1908 128 11 11 3 25 536 105
Cuts at: 2854 2905 127
Size: 51 3179
Fragments arranged by size:
3179 1908 536 128 105 51 25 11
11 3
BseYI C'CCAG_C
Cuts at: 138 1148 1680 2140 2288 2740 3382 4140 4805
Size: 1010 532 460 148 452 642 758 665
Cuts at: 4805 5213 138
Size: 408 882
Fragments arranged by size:
1010 882 758 665 642 532 460 452
408 148
BsiEI CG_ry'CG
Cuts at: 104 3499 3545 4775 104
Size: 3395 46 1230 1286
Fragments arranged by size:
3395 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 212 361 722 1076 2224 2858 2971 3749 3939
Size: 149 361 354 1148 634 113 778 190
Cuts at: 3939 4799 212
Size: 860 1370
Fragments arranged by size:
1370 1148 860 778 634 361 354 190
149 113
BsmI GAATG_Cn'
Cuts at: 2319 2319
Size: 5957
BsmBI CGTCTCn'nnnn_
Cuts at: 1169 1169
Size: 5957
Bsp1286I G_dGCh'C
Cuts at: 212 361 714 722 1076 1408 2224 2353 2741
Size: 149 353 8 354 332 816 129 388
Cuts at: 2741 2767 2858 2971 3582 3675 3749 3939 4001
Size: 26 91 113 611 93 74 190 62
Cuts at: 4001 4799 212
Size: 798 1370
Fragments arranged by size:
1370 816 798 611 388 354 353 332
190 149 129 113 93 91 74 62
26 8
BspHI T'CATG_A
Cuts at: 1892 4389 1892
Size: 2497 3460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 187 2897 3007 3221 3523 3904 187
Size: 2710 110 214 302 381 2240
Fragments arranged by size:
2710 2240 381 302 214 110
BsrBI CCG'CTC
Cuts at: 50 248 2775 4249 4303 5787 5952 50
Size: 198 2527 1474 54 1484 165 55
Fragments arranged by size:
2527 1484 1474 198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1656 1859 3869 1656
Size: 203 2010 3744
Fragments arranged by size:
3744 2010 203
BsrFI r'CCGG_y
Cuts at: 158 2598 3955 4136 158
Size: 2440 1357 181 1979
Fragments arranged by size:
2440 1979 1357 181
BssSI C'ACGA_G
Cuts at: 4228 4936 4228
Size: 708 5249
BstAPI GCAn_nnn'nTGC
Cuts at: 1029 1158 3334 1029
Size: 129 2176 3652
Fragments arranged by size:
3652 2176 129
BstBI TT'CG_AA
Cuts at: 4318 4318
Size: 5957
BstXI CCAn_nnnn'nTGG
Cuts at: 2685 2685
Size: 5957
BstYI r'GATC_y
Cuts at: 1 1731 2050 2982 3449 3472 3807 4053 4359
Size: 1730 319 932 467 23 335 246 306
Cuts at: 4359 4371 4457 5509 1
Size: 12 86 1052 449
Fragments arranged by size:
1730 1052 932 467 449 335 319 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 677 2806 677
Size: 2129 3828
BtgI C'CryG_G
Cuts at: 107 819 1916 1940 2684 107
Size: 712 1097 24 744 3380
Fragments arranged by size:
3380 1097 744 712 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 819 2730 3907 4088 4251 819
Size: 1911 1177 181 163 2525
Fragments arranged by size:
2525 1911 1177 181 163
BtsI GCAGTG_nn'
Cuts at: 2609 2821 3200 2609
Size: 212 379 5366
Fragments arranged by size:
5366 379 212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 939 974 939
Size: 35 5922
DraI TTT'AAA
Cuts at: 2956 2956
Size: 5957
DraIII CAC_nnn'GTG
Cuts at: 371 371
Size: 5957
DrdI GACnn_nn'nnGTC
Cuts at: 163 3663 5007 163
Size: 3500 1344 1113
Fragments arranged by size:
3500 1344 1113
EaeI y'GGCC_r
Cuts at: 496 830 1205 1321 1546 2079 2297 2687 3542
Size: 334 375 116 225 533 218 390 855
Cuts at: 3542 3716 4107 4134 5479 496
Size: 174 391 27 1345 974
Fragments arranged by size:
1345 974 855 533 391 390 375 334
225 218 174 116 27
EagI C'GGCC_G
Cuts at: 3542 3542
Size: 5957
EarI CTCTTCn'nnn_
Cuts at: 884 2140 2303 2587 3980 4190 884
Size: 1256 163 284 1393 210 2651
Fragments arranged by size:
2651 1393 1256 284 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4165 4907 5053 5767 4165
Size: 742 146 714 4355
Fragments arranged by size:
4355 742 714 146
EcoICRI GAG'CTC
Cuts at: 210 1074 2969 210
Size: 864 1895 3198
Fragments arranged by size:
3198 1895 864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 1470 2752 2806 3782 4214 4567 5229 581
Size: 889 1282 54 976 432 353 662 1309
Fragments arranged by size:
1309 1282 976 889 662 432 353 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2809 2809
Size: 5957
EcoO109I rG'GnC_Cy
Cuts at: 2635 3096 2635
Size: 461 5496
EcoRI G'AATT_C
Cuts at: 652 814 1620 2961 652
Size: 162 806 1341 3648
Fragments arranged by size:
3648 1341 806 162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3976 3976
Size: 5957
FspI TGC'GCA
Cuts at: 3738 3738
Size: 5957
HaeII r_GCGC'y
Cuts at: 2654 2888 2934 3391 3639 4869 5640 2654
Size: 234 46 457 248 1230 771 2971
Fragments arranged by size:
2971 1230 771 457 248 234 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 454 486 1921 1953 2042 2074 2076
Size: 32 190 32 1435 32 89 32 2
Cuts at: 2076 2108 3799 3829 3831 3861 232
Size: 32 1691 30 2 30 2328
Fragments arranged by size:
2328 1691 1435 190 89 32 32 32
32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 1536 2996 1536
Size: 1460 4497
HindIII A'AGCT_T
Cuts at: 274 274
Size: 5957
KasI G'GCGC_C
Cuts at: 2650 2884 2930 3387 3635 2650
Size: 234 46 457 248 4972
Fragments arranged by size:
4972 457 248 234 46
KpnI G_GTAC'C
Cuts at: 2836 2977 2836
Size: 141 5816
MluI A'CGCG_T
Cuts at: 5390 5390
Size: 5957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 618 760 787 4716 4900 618
Size: 142 27 3929 184 1675
Fragments arranged by size:
3929 1675 184 142 27
MscI TGG'CCA
Cuts at: 498 832 1207 1323 2081 2299 2689 3718 498
Size: 334 375 116 758 218 390 1029 2737
Fragments arranged by size:
2737 1029 758 390 375 334 218 116
MslI CAynn'nnrTG
Cuts at: 644 1225 1568 1945 3946 4073 644
Size: 581 343 377 2001 127 2528
Fragments arranged by size:
2528 2001 581 377 343 127
MspA1I CmG'CkG
Cuts at: 1158 2292 3068 3382 3742 4526 4771 1158
Size: 1134 776 314 360 784 245 2344
Fragments arranged by size:
2344 1134 784 776 360 314 245
NaeI GCC'GGC
Cuts at: 4138 4138
Size: 5957
NarI GG'CG_CC
Cuts at: 2651 2885 2931 3388 3636 2651
Size: 234 46 457 248 4972
Fragments arranged by size:
4972 457 248 234 46
NcoI C'CATG_G
Cuts at: 107 1940 107
Size: 1833 4124
NgoMIV G'CCGG_C
Cuts at: 4136 4136
Size: 5957
NspI r_CATG'y
Cuts at: 2399 3010 3510 5113 2399
Size: 611 500 1603 3243
Fragments arranged by size:
3243 1603 611 500
PciI A'CATG_T
Cuts at: 5109 5109
Size: 5957
PflMI CCAn_nnn'nTGG
Cuts at: 1745 2295 1745
Size: 550 5407
PfoI T'CCnGG_A
Cuts at: 203 932 1744 203
Size: 729 812 4416
Fragments arranged by size:
4416 812 729
PmeI GTTT'AAAC
Cuts at: 2956 2956
Size: 5957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2469 4403 4435 2437
Size: 32 1934 32 3959
Fragments arranged by size:
3959 1934 32 32
PpuMI rG'GwC_Cy
Cuts at: 2635 2635
Size: 5957
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 961 993 961
Size: 32 5925
PstI C_TGCA'G
Cuts at: 2645 2828 2928 3004 3689 2645
Size: 183 100 76 685 4913
Fragments arranged by size:
4913 685 183 100 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5957
PvuII CAG'CTG
Cuts at: 1158 2292 3382 3742 1158
Size: 1134 1090 360 3373
Fragments arranged by size:
3373 1134 1090 360
RsrII CG'GwC_CG
Cuts at: 4152 4152
Size: 5957
SacI G_AGCT'C
Cuts at: 212 1076 2971 212
Size: 864 1895 3198
Fragments arranged by size:
3198 1895 864
SalI G'TCGA_C
Cuts at: 2994 2994
Size: 5957
SapI GCTCTTCn'nnn_
Cuts at: 3980 4190 3980
Size: 210 5747
SbfI CC_TGCA'GG
Cuts at: 2645 3004 2645
Size: 359 5598
ScaI AGT'ACT
Cuts at: 2485 5474 2485
Size: 2989 2968
SfcI C'TryA_G
Cuts at: 33 1658 2641 2824 2924 3000 3685 4653 4844
Size: 1625 983 183 100 76 685 968 191
Cuts at: 4844 33
Size: 1146
Fragments arranged by size:
1625 1146 983 968 685 191 183 100
76
SfoI GGC'GCC
Cuts at: 2652 2886 2932 3389 3637 2652
Size: 234 46 457 248 4972
Fragments arranged by size:
4972 457 248 234 46
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
SmaI CCC'GGG
Cuts at: 1345 2979 1345
Size: 1634 4323
SmlI C'TyrA_G
Cuts at: 193 265 3112 4027 4741 5003 193
Size: 72 2847 915 714 262 1147
Fragments arranged by size:
2847 1147 915 714 262 72
SpeI A'CTAG_T
Cuts at: 67 1281 67
Size: 1214 4743
SphI G_CATG'C
Cuts at: 3010 3510 3010
Size: 500 5457
StyI C'CwwG_G
Cuts at: 107 437 624 657 791 1250 1940 2003 2108
Size: 330 187 33 134 459 690 63 105
Cuts at: 2108 3091 3445 107
Size: 983 354 2619
Fragments arranged by size:
2619 983 690 459 354 330 187 134
105 63 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 517 2223 3540 3673 4132 4341 517
Size: 1706 1317 133 459 209 2133
Fragments arranged by size:
2133 1706 1317 459 209 133
TatI w'GTAC_w
Cuts at: 1493 2276 2483 5472 1493
Size: 783 207 2989 1978
Fragments arranged by size:
2989 1978 783 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1503 1535 5474 5506 1503
Size: 32 3939 32 1954
Fragments arranged by size:
3939 1954 32 32
Tth111I GACn'n_nGTC
Cuts at: 1507 3754 1507
Size: 2247 3710
XbaI T'CTAG_A
Cuts at: 2988 2988
Size: 5957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3452 3452
Size: 5957
XmaI C'CCGG_G
Cuts at: 1343 2977 1343
Size: 1634 4323
XmnI GAAnn'nnTTC
Cuts at: 800 814 2365 800
Size: 14 1551 4392
Fragments arranged by size:
4392 1551 14
ZraI GAC'GTC
Cuts at: 2898 2898
Size: 5957
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
BaeI BbsI BfrBI BmtI BsgI BsiWI BspEI BsrGI
BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI