Restriction map for
pF1KA1333
(Circular) (Six-base) MAPSORT of: pF1KA1333.seq Check: 9092 from: 1 to: 5231
pF1KA1333 5231 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2269 2269
Size: 5231
Acc65I G'GTAC_C
Cuts at: 2247 2247
Size: 5231
AclI AA'CG_TT
Cuts at: 5071 5071
Size: 5231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 3056 3488 4235 4503 415
Size: 2641 432 747 268 1143
Fragments arranged by size:
2641 1143 747 432 268
AfeI AGC'GCT
Cuts at: 877 877
Size: 5231
AflIII A'CryG_T
Cuts at: 1008 2223 2583 3687 4664 1008
Size: 1215 360 1104 977 1575
Fragments arranged by size:
1575 1215 1104 977 360
AgeI A'CCGG_T
Cuts at: 3619 3619
Size: 5231
AhdI GACnn_n'nnGTC
Cuts at: 1638 1638
Size: 5231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2037 2037
Size: 5231
AlwNI CAG_nnn'CTG
Cuts at: 1794 2536 4103 1794
Size: 742 1567 2922
Fragments arranged by size:
2922 1567 742
ApaLI G'TGCA_C
Cuts at: 4001 4001
Size: 5231
ApoI r'AATT_y
Cuts at: 257 553 725 1129 1339 1416 1820 2235 4584
Size: 296 172 404 210 77 404 415 2349
Cuts at: 4584 4840 257
Size: 256 648
Fragments arranged by size:
2349 648 415 404 404 296 256 210
172 77
AseI AT'TA_AT
Cuts at: 20 1156 20
Size: 1136 4095
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
AvaI C'yCGr_G
Cuts at: 2251 2251
Size: 5231
BamHI G'GATC_C
Cuts at: 1093 2256 1093
Size: 1163 4068
BanI G'GyrC_C
Cuts at: 2247 2661 2909 2944 2247
Size: 414 248 35 4534
Fragments arranged by size:
4534 414 248 35
BanII G_rGCy'C
Cuts at: 2245 3275 2245
Size: 1030 4201
BbeI G_GCGC'C
Cuts at: 2665 2913 2665
Size: 248 4983
BbsI GAAGACnn'nnnn_
Cuts at: 2003 2003
Size: 5231
BciVI GTATCCnnnnn_n'
Cuts at: 3129 3890 5231 3129
Size: 761 1341 3129
Fragments arranged by size:
3129 1341 761
BclI T'GATC_A
Cuts at: 2751 2751
Size: 5231
BfrBI ATG'CAT
Cuts at: 1240 1240
Size: 5231
BglII A'GATC_T
Cuts at: 1107 2746 4783 1107
Size: 1639 2037 1555
Fragments arranged by size:
2037 1639 1555
BlpI GC'TnA_GC
Cuts at: 2343 2343
Size: 5231
Bme1580I G_kGCm'C
Cuts at: 2856 2949 4005 2856
Size: 93 1056 4082
Fragments arranged by size:
4082 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2443 2524 2859 5003 2443
Size: 81 335 2144 2671
Fragments arranged by size:
2671 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3598 3598
Size: 5231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2021
Size: 5231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 1406 1690 2407 3286 3778 4076 1387
Size: 19 284 717 879 492 298 2542
Fragments arranged by size:
2542 879 717 492 298 284 19
BsaI GGTCTCn'nnnn_
Cuts at: 4935 4935
Size: 5231
BsaAI yAC'GTr
Cuts at: 2584 2584
Size: 5231
BsaBI GATnn'nnATC
Cuts at: 6 1600 2745 4782 6
Size: 1594 1145 2037 455
Fragments arranged by size:
2037 1594 1145 455
BsaHI Gr'CG_yC
Cuts at: 2662 2910 3612 5108 2662
Size: 248 702 1496 2785
Fragments arranged by size:
2785 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2420 2941 3619 3893 4040 4451 2420
Size: 521 678 274 147 411 3200
Fragments arranged by size:
3200 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 909 909
Size: 5231
BseYI C'CCAG_C
Cuts at: 1553 2656 3414 3991 4487 1553
Size: 1103 758 577 496 2297
Fragments arranged by size:
2297 1103 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2773 2819 4027 104
Size: 2669 46 1208 1308
Fragments arranged by size:
2669 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2245 3023 3213 4005 2245
Size: 778 190 792 3471
Fragments arranged by size:
3471 792 778 190
BsmI GAATG_Cn'
Cuts at: 1240 1660 1240
Size: 420 4811
Bsp1286I G_dGCh'C
Cuts at: 2245 2856 2949 3023 3213 3275 4005 2245
Size: 611 93 74 190 62 730 3471
Fragments arranged by size:
3471 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4451 4451
Size: 5231
BspHI T'CATG_A
Cuts at: 4407 4407
Size: 5231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2281 2495 2797 3178 2281
Size: 214 302 381 4334
Fragments arranged by size:
4334 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3523 3577 5061 5226 50
Size: 3473 54 1484 165 55
Fragments arranged by size:
3473 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3143 3143
Size: 5231
BsrFI r'CCGG_y
Cuts at: 3229 3410 3619 3229
Size: 181 209 4841
Fragments arranged by size:
4841 209 181
BsrGI T'GTAC_A
Cuts at: 225 673 225
Size: 448 4783
BssSI C'ACGA_G
Cuts at: 3502 3860 3502
Size: 358 4873
BstAPI GCAn_nnn'nTGC
Cuts at: 545 649 2608 545
Size: 104 1959 3168
Fragments arranged by size:
3168 1959 104
BstBI TT'CG_AA
Cuts at: 813 3592 813
Size: 2779 2452
BstXI CCAn_nnnn'nTGG
Cuts at: 457 549 457
Size: 92 5139
BstYI r'GATC_y
Cuts at: 1 1093 1107 2256 2723 2746 3081 3327 4339
Size: 1092 14 1149 467 23 335 246 1012
Cuts at: 4339 4425 4437 4783 1
Size: 86 12 346 449
Fragments arranged by size:
1149 1092 1012 467 449 346 335 246
86 23 14 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3181 3362 3525 3181
Size: 181 163 4887
Fragments arranged by size:
4887 181 163
BtsI GCAGTG_nn'
Cuts at: 2474 2474
Size: 5231
DraI TTT'AAA
Cuts at: 2049 2230 2049
Size: 181 5050
DrdI GACnn_nn'nnGTC
Cuts at: 2937 3795 2937
Size: 858 4373
EaeI y'GGCC_r
Cuts at: 2816 2990 3381 3408 4753 2816
Size: 174 391 27 1345 3294
Fragments arranged by size:
3294 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2816 2816
Size: 5231
EarI CTCTTCn'nnn_
Cuts at: 1052 3254 3464 1052
Size: 2202 210 2819
Fragments arranged by size:
2819 2202 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3439 3749 3895 5041 3439
Size: 310 146 1146 3629
Fragments arranged by size:
3629 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2243 2243
Size: 5231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 2021 3056 3488 4235 4503 415
Size: 1606 1035 432 747 268 1143
Fragments arranged by size:
1606 1143 1035 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1391 2370 1391
Size: 979 4252
EcoRI G'AATT_C
Cuts at: 725 2235 725
Size: 1510 3721
EcoRV GAT'ATC
Cuts at: 312 312
Size: 5231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1456 1753 2020 3250 1456
Size: 297 267 1230 3437
Fragments arranged by size:
3437 1230 297 267
FspI TGC'GCA
Cuts at: 3012 3012
Size: 5231
HaeII r_GCGC'y
Cuts at: 879 2665 2913 3935 4914 879
Size: 1786 248 1022 979 1196
Fragments arranged by size:
1786 1196 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1092 1124 1187 1219 1360 1392 1624 1656 1963
Size: 32 63 32 141 32 232 32 307
Cuts at: 1963 1995 3073 3103 3105 3135 1092
Size: 32 1078 30 2 30 3188
Fragments arranged by size:
3188 1078 307 232 141 63 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2270 2270
Size: 5231
HindIII A'AGCT_T
Cuts at: 1931 1931
Size: 5231
KasI G'GCGC_C
Cuts at: 2661 2909 2661
Size: 248 4983
KpnI G_GTAC'C
Cuts at: 2251 2251
Size: 5231
MfeI C'AATT_G
Cuts at: 377 1274 2063 377
Size: 897 789 3545
Fragments arranged by size:
3545 897 789
MluI A'CGCG_T
Cuts at: 4664 4664
Size: 5231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1034 2073 3902 4086 1034
Size: 1039 1829 184 2179
Fragments arranged by size:
2179 1829 1039 184
MscI TGG'CCA
Cuts at: 2992 2992
Size: 5231
MslI CAynn'nnrTG
Cuts at: 396 3220 3347 396
Size: 2824 127 2280
Fragments arranged by size:
2824 2280 127
MspA1I CmG'CkG
Cuts at: 2041 2342 2656 3016 4029 4274 2041
Size: 301 314 360 1013 245 2998
Fragments arranged by size:
2998 1013 360 314 301 245
NaeI GCC'GGC
Cuts at: 3412 3412
Size: 5231
NarI GG'CG_CC
Cuts at: 2662 2910 2662
Size: 248 4983
NdeI CA'TA_TG
Cuts at: 1269 1287 1269
Size: 18 5213
NgoMIV G'CCGG_C
Cuts at: 3410 3410
Size: 5231
NsiI A_TGCA'T
Cuts at: 1242 1242
Size: 5231
NspI r_CATG'y
Cuts at: 1012 2080 2227 2284 2784 3691 1012
Size: 1068 147 57 500 907 2552
Fragments arranged by size:
2552 1068 907 500 147 57
PciI A'CATG_T
Cuts at: 1008 2223 3687 1008
Size: 1215 1464 2552
Fragments arranged by size:
2552 1464 1215
PflMI CCAn_nnn'nTGG
Cuts at: 1220 1220
Size: 5231
PfoI T'CCnGG_A
Cuts at: 1323 1323
Size: 5231
PmeI GTTT'AAAC
Cuts at: 2230 2230
Size: 5231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 4370
Size: 32 5199
PpuMI rG'GwC_Cy
Cuts at: 1391 1391
Size: 5231
PsiI TTA'TAA
Cuts at: 578 1600 1696 578
Size: 1022 96 4113
Fragments arranged by size:
4113 1022 96
PstI C_TGCA'G
Cuts at: 791 2278 2963 791
Size: 1487 685 3059
Fragments arranged by size:
3059 1487 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5231
PvuII CAG'CTG
Cuts at: 2041 2656 3016 2041
Size: 615 360 4256
Fragments arranged by size:
4256 615 360
RsrII CG'GwC_CG
Cuts at: 3426 3426
Size: 5231
SacI G_AGCT'C
Cuts at: 2245 2245
Size: 5231
SalI G'TCGA_C
Cuts at: 2268 2268
Size: 5231
SapI GCTCTTCn'nnn_
Cuts at: 3254 3464 3254
Size: 210 5021
SbfI CC_TGCA'GG
Cuts at: 2278 2278
Size: 5231
ScaI AGT'ACT
Cuts at: 4748 4748
Size: 5231
SexAI A'CCwGG_T
Cuts at: 123 1497 123
Size: 1374 3857
SfcI C'TryA_G
Cuts at: 33 787 1625 2274 2959 3952 4143 33
Size: 754 838 649 685 993 191 1121
Fragments arranged by size:
1121 993 838 754 685 649 191
SfoI GGC'GCC
Cuts at: 2663 2911 2663
Size: 248 4983
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5231
SmaI CCC'GGG
Cuts at: 2253 2253
Size: 5231
SmlI C'TyrA_G
Cuts at: 1366 1421 1669 2386 3301 3793 4055 1366
Size: 55 248 717 915 492 262 2542
Fragments arranged by size:
2542 915 717 492 262 248 55
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5231
SphI G_CATG'C
Cuts at: 2284 2784 2284
Size: 500 4731
StyI C'CwwG_G
Cuts at: 1803 2365 2719 1803
Size: 562 354 4315
Fragments arranged by size:
4315 562 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2814 2947 3406 3615 2814
Size: 133 459 209 4430
Fragments arranged by size:
4430 459 209 133
TatI w'GTAC_w
Cuts at: 225 673 1908 4746 225
Size: 448 1235 2838 710
Fragments arranged by size:
2838 1235 710 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4748 4780 4748
Size: 32 5199
Tth111I GACn'n_nGTC
Cuts at: 3028 3028
Size: 5231
XbaI T'CTAG_A
Cuts at: 2262 2262
Size: 5231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2726 2726
Size: 5231
XmaI C'CCGG_G
Cuts at: 2251 2251
Size: 5231
XmnI GAAnn'nnTTC
Cuts at: 2000 2000
Size: 5231
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI FseI FspAI HpaI NcoI
NheI NotI NruI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI