Restriction map for
pF1KA1315
(Circular) (Six-base) MAPSORT of: pF1KA1315.seq Check: 9361 from: 1 to: 9056
pF1KA1315 9056 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1112 4342 6094 1112
Size: 3230 1752 4074
Fragments arranged by size:
4074 3230 1752
Acc65I G'GTAC_C
Cuts at: 6072 6072
Size: 9056
AclI AA'CG_TT
Cuts at: 1996 8896 1996
Size: 6900 2156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1535 1718 2357 2539 4265 4477 4535 4991
Size: 1218 183 639 182 1726 212 58 456
Cuts at: 4991 5741 6881 7313 7666 8328 317
Size: 750 1140 432 353 662 1045
Fragments arranged by size:
1726 1218 1140 1045 750 662 639 456
432 353 212 183 182 58
AfeI AGC'GCT
Cuts at: 237 3862 237
Size: 3625 5431
AflII C'TTAA_G
Cuts at: 172 439 903 3427 3544 4153 172
Size: 267 464 2524 117 609 5075
Fragments arranged by size:
5075 2524 609 464 267 117
AflIII A'CryG_T
Cuts at: 2878 3066 3227 3249 6408 8208 8489 2878
Size: 188 161 22 3159 1800 281 3445
Fragments arranged by size:
3445 3159 1800 281 188 161 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 9056
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3835 3867 3835
Size: 32 9024
AlwNI CAG_nnn'CTG
Cuts at: 1039 2032 2239 5182 6361 7799 1039
Size: 993 207 2943 1179 1438 2296
Fragments arranged by size:
2943 2296 1438 1179 993 207
ApaLI G'TGCA_C
Cuts at: 7894 7894
Size: 9056
ApoI r'AATT_y
Cuts at: 408 2461 2784 2886 2967 3271 3283 3813 4990
Size: 2053 323 102 81 304 12 530 1177
Cuts at: 4990 5203 5912 6060 8409 8665 408
Size: 213 709 148 2349 256 799
Fragments arranged by size:
2349 2053 1177 799 709 530 323 304
256 213 148 102 81 12
AseI AT'TA_AT
Cuts at: 20 2216 3292 20
Size: 2196 1076 5784
Fragments arranged by size:
5784 2196 1076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9056
AvaI C'yCGr_G
Cuts at: 6076 6076
Size: 9056
BamHI G'GATC_C
Cuts at: 6081 6081
Size: 9056
BanI G'GyrC_C
Cuts at: 6072 6486 6734 6769 6072
Size: 414 248 35 8359
Fragments arranged by size:
8359 414 248 35
BanII G_rGCy'C
Cuts at: 2126 4957 6070 7100 2126
Size: 2831 1113 1030 4082
Fragments arranged by size:
4082 2831 1113 1030
BbeI G_GCGC'C
Cuts at: 6490 6738 6490
Size: 248 8808
BbsI GAAGACnn'nnnn_
Cuts at: 1609 1886 2075 3713 4271 5390 1609
Size: 277 189 1638 558 1119 5275
Fragments arranged by size:
5275 1638 1119 558 277 189
BciVI GTATCCnnnnn_n'
Cuts at: 2289 6954 8010 9056 2289
Size: 4665 1056 1046 2289
Fragments arranged by size:
4665 2289 1056 1046
BclI T'GATC_A
Cuts at: 639 1212 4722 5232 6576 639
Size: 573 3510 510 1344 3119
Fragments arranged by size:
3510 3119 1344 573 510
BfrBI ATG'CAT
Cuts at: 1470 2112 4570 4695 5468 1470
Size: 642 2458 125 773 5058
Fragments arranged by size:
5058 2458 773 642 125
BglII A'GATC_T
Cuts at: 594 607 1258 3124 4422 5160 6571 8608 594
Size: 13 651 1866 1298 738 1411 2037 1042
Fragments arranged by size:
2037 1866 1411 1298 1042 738 651 13
BlpI GC'TnA_GC
Cuts at: 6168 6168
Size: 9056
Bme1580I G_kGCm'C
Cuts at: 6681 6774 7898 6681
Size: 93 1124 7839
Fragments arranged by size:
7839 1124 93
BmgBI CAC'GTC
Cuts at: 3022 4747 3022
Size: 1725 7331
BmrI ACTGGGnnnn_n'
Cuts at: 5504 6268 6349 6684 8828 5504
Size: 764 81 335 2144 5732
Fragments arranged by size:
5732 2144 764 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 837 7423 837
Size: 6586 2470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1272 2536 3762 4112 4618 4646 5270 1109
Size: 163 1264 1226 350 506 28 624 4895
Fragments arranged by size:
4895 1264 1226 624 506 350 163 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 4576 6232 7111 7825 8123 2723
Size: 1853 1656 879 714 298 3656
Fragments arranged by size:
3656 1853 1656 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1075 2652 2694 6003 8760 1075
Size: 1577 42 3309 2757 1371
Fragments arranged by size:
3309 2757 1577 1371 42
BsaAI yAC'GTr
Cuts at: 5157 6409 5157
Size: 1252 7804
BsaBI GATnn'nnATC
Cuts at: 6 674 1407 2047 4469 5630 5984 6570 8607
Size: 668 733 640 2422 1161 354 586 2037
Cuts at: 8607 6
Size: 455
Fragments arranged by size:
2422 2037 1161 733 668 640 586 455
354
BsaHI Gr'CG_yC
Cuts at: 6487 6735 7437 8933 6487
Size: 248 702 1496 6610
Fragments arranged by size:
6610 1496 702 248
BsaWI w'CCGG_w
Cuts at: 6245 6766 7444 7855 8002 8276 6245
Size: 521 678 411 147 274 7025
Fragments arranged by size:
7025 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1791 1821 5490 5520 1791
Size: 30 3669 30 5327
Fragments arranged by size:
5327 3669 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 158 158
Size: 9056
BseYI C'CCAG_C
Cuts at: 208 3941 6481 7239 7904 8312 208
Size: 3733 2540 758 665 408 952
Fragments arranged by size:
3733 2540 952 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 756
Size: 9056
BsiEI CG_ry'CG
Cuts at: 104 6598 6644 7874 104
Size: 6494 46 1230 1286
Fragments arranged by size:
6494 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3163 4533 4957 5274 6070 6848 7038 7898 3163
Size: 1370 424 317 796 778 190 860 4321
Fragments arranged by size:
4321 1370 860 796 778 424 317 190
BsmI GAATG_Cn'
Cuts at: 3630 3630
Size: 9056
Bsp1286I G_dGCh'C
Cuts at: 2126 3163 4533 4957 5274 6070 6681 6774 6848
Size: 1037 1370 424 317 796 611 93 74
Cuts at: 6848 7038 7100 7898 2126
Size: 190 62 798 3284
Fragments arranged by size:
3284 1370 1037 798 796 611 424 317
190 93 74 62
BspHI T'CATG_A
Cuts at: 2613 4941 7488 2613
Size: 2328 2547 4181
Fragments arranged by size:
4181 2547 2328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 6106 6320 6622 7003 6106
Size: 214 302 381 8159
Fragments arranged by size:
8159 381 302 214
BsrBI CCG'CTC
Cuts at: 50 7348 7402 8886 9051 50
Size: 7298 54 1484 165 55
Fragments arranged by size:
7298 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 4291 6968 1730
Size: 2561 2677 3818
Fragments arranged by size:
3818 2677 2561
BsrFI r'CCGG_y
Cuts at: 4296 7054 7235 4296
Size: 2758 181 6117
Fragments arranged by size:
6117 2758 181
BsrGI T'GTAC_A
Cuts at: 672 2863 4177 672
Size: 2191 1314 5551
Fragments arranged by size:
5551 2191 1314
BssSI C'ACGA_G
Cuts at: 7327 8035 7327
Size: 708 8348
BstAPI GCAn_nnn'nTGC
Cuts at: 4397 5824 6433 4397
Size: 1427 609 7020
Fragments arranged by size:
7020 1427 609
BstBI TT'CG_AA
Cuts at: 546 1015 7417 546
Size: 469 6402 2185
Fragments arranged by size:
6402 2185 469
BstXI CCAn_nnnn'nTGG
Cuts at: 5196 5196
Size: 9056
BstYI r'GATC_y
Cuts at: 1 287 594 607 1258 3124 4422 5160 5172
Size: 286 307 13 651 1866 1298 738 12
Cuts at: 5172 5772 6081 6548 6571 6906 7152 7458 7470
Size: 600 309 467 23 335 246 306 12
Cuts at: 7470 7556 8608 1
Size: 86 1052 449
Fragments arranged by size:
1866 1298 1052 738 651 600 467 449
335 309 307 306 286 246 86 23
13 12 12
BtgI C'CryG_G
Cuts at: 107 1976 107
Size: 1869 7187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4433 7006 7187 7350 4433
Size: 2573 181 163 6139
Fragments arranged by size:
6139 2573 181 163
BtsI GCAGTG_nn'
Cuts at: 688 768 2033 4092 6299 688
Size: 80 1265 2059 2207 3445
Fragments arranged by size:
3445 2207 2059 1265 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1854 1889 1854
Size: 35 9021
DraI TTT'AAA
Cuts at: 390 1023 1493 1640 4050 4484 4884 5261 5462
Size: 633 470 147 2410 434 400 377 201
Cuts at: 5462 5949 6055 390
Size: 487 106 3391
Fragments arranged by size:
3391 2410 633 487 470 434 400 377
201 147 106
DraIII CAC_nnn'GTG
Cuts at: 3022 3022
Size: 9056
DrdI GACnn_nn'nnGTC
Cuts at: 6762 8106 6762
Size: 1344 7712
EaeI y'GGCC_r
Cuts at: 6641 6815 7206 7233 8578 6641
Size: 174 391 27 1345 7119
Fragments arranged by size:
7119 1345 391 174 27
EagI C'GGCC_G
Cuts at: 6641 6641
Size: 9056
EarI CTCTTCn'nnn_
Cuts at: 278 834 1071 1328 1335 2024 2114 3210 3576
Size: 556 237 257 7 689 90 1096 366
Cuts at: 3576 3851 4181 4556 4770 7079 7289 278
Size: 275 330 375 214 2309 210 2045
Fragments arranged by size:
2309 2045 1096 689 556 375 366 330
275 257 237 214 210 90 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7264 8006 8152 8866 7264
Size: 742 146 714 7454
Fragments arranged by size:
7454 742 714 146
EcoICRI GAG'CTC
Cuts at: 4955 6068 4955
Size: 1113 7943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1109 1272 1535 1718 2357 2536 2539 3762
Size: 792 163 263 183 639 179 3 1223
Cuts at: 3762 4112 4265 4477 4535 4618 4646 4991 5270
Size: 350 153 212 58 83 28 345 279
Cuts at: 5270 5741 6881 7313 7666 8328 317
Size: 471 1140 432 353 662 1045
Fragments arranged by size:
1223 1140 1045 792 662 639 471 432
353 350 345 279 263 212 183 179
163 153 83 58 28 3
EcoO109I rG'GnC_Cy
Cuts at: 1764 5754 6195 1764
Size: 3990 441 4625
Fragments arranged by size:
4625 3990 441
EcoRI G'AATT_C
Cuts at: 2886 5203 6060 2886
Size: 2317 857 5882
Fragments arranged by size:
5882 2317 857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 755 3570 3619 4649 7075 755
Size: 2815 49 1030 2426 2736
Fragments arranged by size:
2815 2736 2426 1030 49
FspI TGC'GCA
Cuts at: 6837 6837
Size: 9056
HaeII r_GCGC'y
Cuts at: 239 3864 6490 6738 7968 8739 239
Size: 3625 2626 248 1230 771 556
Fragments arranged by size:
3625 2626 1230 771 556 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 586 618 1876 1908 2488 2520 2611
Size: 32 379 32 1258 32 580 32 91
Cuts at: 2611 2643 4228 4260 4483 4515 4555 4587 5527
Size: 32 1585 32 223 32 40 32 940
Cuts at: 5527 5539 5559 5571 5757 5764 5789 5796 5864
Size: 12 20 12 186 7 25 7 68
Cuts at: 5864 5896 6898 6928 6930 6960 175
Size: 32 1002 30 2 30 2271
Fragments arranged by size:
2271 1585 1258 1002 940 580 379 223
186 91 68 40 32 32 32 32
32 32 32 32 32 30 30 25
20 12 12 7 7 2
HincII GTy'rAC
Cuts at: 358 1994 2100 4092 4937 6095 358
Size: 1636 106 1992 845 1158 3319
Fragments arranged by size:
3319 1992 1636 1158 845 106
HindIII A'AGCT_T
Cuts at: 3541 3573 3541
Size: 32 9024
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 9056
KasI G'GCGC_C
Cuts at: 6486 6734 6486
Size: 248 8808
KpnI G_GTAC'C
Cuts at: 6076 6076
Size: 9056
MfeI C'AATT_G
Cuts at: 2408 2408
Size: 9056
MluI A'CGCG_T
Cuts at: 8489 8489
Size: 9056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3692 4295 4934 5957 7815 7999 194
Size: 3498 603 639 1023 1858 184 1251
Fragments arranged by size:
3498 1858 1251 1023 639 603 184
MscI TGG'CCA
Cuts at: 6817 6817
Size: 9056
MslI CAynn'nnrTG
Cuts at: 620 2618 2768 3226 4307 4538 5027 7045 7172
Size: 1998 150 458 1081 231 489 2018 127
Cuts at: 7172 620
Size: 2504
Fragments arranged by size:
2504 2018 1998 1081 489 458 231 150
127
MspA1I CmG'CkG
Cuts at: 1960 2989 4140 6167 6481 6841 7625 7870 1960
Size: 1029 1151 2027 314 360 784 245 3146
Fragments arranged by size:
3146 2027 1151 1029 784 360 314 245
NaeI GCC'GGC
Cuts at: 7237 7237
Size: 9056
NarI GG'CG_CC
Cuts at: 6487 6735 6487
Size: 248 8808
NcoI C'CATG_G
Cuts at: 107 1976 107
Size: 1869 7187
NdeI CA'TA_TG
Cuts at: 1920 1920
Size: 9056
NgoMIV G'CCGG_C
Cuts at: 7235 7235
Size: 9056
NsiI A_TGCA'T
Cuts at: 1472 2114 4572 4697 5470 1472
Size: 642 2458 125 773 5058
Fragments arranged by size:
5058 2458 773 642 125
NspI r_CATG'y
Cuts at: 2882 3070 3231 3253 3949 4699 6109 6609 8212
Size: 188 161 22 696 750 1410 500 1603
Cuts at: 8212 2882
Size: 3726
Fragments arranged by size:
3726 1603 1410 750 696 500 188 161
22
PacI TTA_AT'TAA
Cuts at: 3347 3347
Size: 9056
PasI CC'CwG_GG
Cuts at: 5797 5797
Size: 9056
PciI A'CATG_T
Cuts at: 2878 3066 3227 3249 8208 2878
Size: 188 161 22 4959 3726
Fragments arranged by size:
4959 3726 188 161 22
PflMI CCAn_nnn'nTGG
Cuts at: 2583 3022 2583
Size: 439 8617
PfoI T'CCnGG_A
Cuts at: 4639 4639
Size: 9056
PmeI GTTT'AAAC
Cuts at: 6055 6055
Size: 9056
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7502 7534 7502
Size: 32 9024
PpuMI rG'GwC_Cy
Cuts at: 1764 1764
Size: 9056
PsiI TTA'TAA
Cuts at: 3722 3722
Size: 9056
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2699 2731 3461 3493 2699
Size: 32 730 32 8262
Fragments arranged by size:
8262 730 32 32
PstI C_TGCA'G
Cuts at: 6103 6788 6103
Size: 685 8371
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9056
PvuII CAG'CTG
Cuts at: 1960 2989 4140 6481 6841 1960
Size: 1029 1151 2341 360 4175
Fragments arranged by size:
4175 2341 1151 1029 360
RsrII CG'GwC_CG
Cuts at: 7251 7251
Size: 9056
SacI G_AGCT'C
Cuts at: 4957 6070 4957
Size: 1113 7943
SalI G'TCGA_C
Cuts at: 6093 6093
Size: 9056
SapI GCTCTTCn'nnn_
Cuts at: 2024 2114 3851 4181 7079 7289 2024
Size: 90 1737 330 2898 210 3791
Fragments arranged by size:
3791 2898 1737 330 210 90
SbfI CC_TGCA'GG
Cuts at: 6103 6103
Size: 9056
ScaI AGT'ACT
Cuts at: 738 909 3033 8573 738
Size: 171 2124 5540 1221
Fragments arranged by size:
5540 2124 1221 171
SfcI C'TryA_G
Cuts at: 33 1586 2705 5306 6099 6784 7752 7943 33
Size: 1553 1119 2601 793 685 968 191 1146
Fragments arranged by size:
2601 1553 1146 1119 968 793 685 191
SfoI GGC'GCC
Cuts at: 6488 6736 6488
Size: 248 8808
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9056
SmaI CCC'GGG
Cuts at: 6078 6078
Size: 9056
SmlI C'TyrA_G
Cuts at: 172 439 903 2738 3427 3544 4153 4555 6211
Size: 267 464 1835 689 117 609 402 1656
Cuts at: 6211 7126 7840 8102 172
Size: 915 714 262 1126
Fragments arranged by size:
1835 1656 1126 915 714 689 609 464
402 267 262 117
SnaBI TAC'GTA
Cuts at: 5157 5157
Size: 9056
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9056
SphI G_CATG'C
Cuts at: 3949 4699 6109 6609 3949
Size: 750 1410 500 6396
Fragments arranged by size:
6396 1410 750 500
SspI AAT'ATT
Cuts at: 450 4046 5279 450
Size: 3596 1233 4227
Fragments arranged by size:
4227 3596 1233
StuI AGG'CCT
Cuts at: 5457 5457
Size: 9056
StyI C'CwwG_G
Cuts at: 107 931 1976 3866 6190 6544 107
Size: 824 1045 1890 2324 354 2619
Fragments arranged by size:
2619 2324 1890 1045 824 354
SwaI ATTT'AAAT
Cuts at: 5261 5261
Size: 9056
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4027 6639 6772 7231 7440 4027
Size: 2612 133 459 209 5643
Fragments arranged by size:
5643 2612 459 209 133
TatI w'GTAC_w
Cuts at: 672 736 907 2863 3031 3873 4096 4177 8571
Size: 64 171 1956 168 842 223 81 4394
Cuts at: 8571 672
Size: 1157
Fragments arranged by size:
4394 1956 1157 842 223 171 168 81
64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5384 5416 5490 5522 8573 8605 5384
Size: 32 74 32 3051 32 5835
Fragments arranged by size:
5835 3051 74 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6853 6853
Size: 9056
XbaI T'CTAG_A
Cuts at: 604 1329 2541 6087 604
Size: 725 1212 3546 3573
Fragments arranged by size:
3573 3546 1212 725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6551 6551
Size: 9056
XmaI C'CCGG_G
Cuts at: 6076 6076
Size: 9056
XmnI GAAnn'nnTTC
Cuts at: 5141 5619 5141
Size: 478 8578
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI BsmBI
BspEI BssHII BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV
FseI FspAI NheI NotI NruI PmlI PshAI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SrfI XhoI
ZraI